Relevant bibliographies by topics / Reverse phase HPLC

Contents

  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers
  6. Reports

Academic literature on the topic 'Reverse phase HPLC'

Author: Grafiati
Published: 3 June 2025
Last updated: 31 July 2025

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Journal articles on the topic "Reverse phase HPLC"

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Kumar, Deepak, Amrendra Kumar, Vinay Kumar, et al. "A Comprehensive Review on Analytical Method Development using RP-HPLC and Recent Advances in Pharmaceutical Applications." Journal for Research in Applied Sciences and Biotechnology 2, no. 2 (2023): 53–60. http://dx.doi.org/10.55544/jrasb.2.2.9.

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The analytical technique of choice for separating, identifying, and quantifying complex mixtures is high-performance liquid chromatography (HPLC). Reverse-phase liquid chromatography (RP-HPLC) is the preferred separation mode for high-performance liquid chromatography (HPLC) due to its adaptability and higher selectivity for hydrophobic compounds. This review article discusses the fundamentals of reversed-phase high-performance liquid chromatography (RP-HPLC). This covers the separation principle, various stationary and mobile phase types, and separation-affecting variables. This article highl
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Hooper, NM. "How to use reverse-phase HPLC." Biochemical Education 20, no. 4 (1992): 243. http://dx.doi.org/10.1016/0307-4412(92)90215-8.

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Abbott, R. W. "How to use reverse-phase HPLC." Analytica Chimica Acta 284, no. 1 (1993): 243. http://dx.doi.org/10.1016/0003-2670(93)80034-i.

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Parmar, Raghuveersinh, Dr Priyanka Patil, Dr Chainesh Shah, and Dr Umesh Upadhyay. "A Review Article on Method Development and Validation of Verapamil by RP-HPLC Method." International Journal of Pharmaceutical Research and Applications 09, no. 05 (2024): 977–88. https://doi.org/10.35629/4494-0905977988.

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High performance liquid chromatography, one of the most powerful analytical techniques utilized in the separation, identification, and quantification of complex mixtures. Reverse-phase or RP-HPLC is commonly practiced in high performance liquid chromatography because this technique has the advantages of versatility and appropriateness for more hydrophobic compounds. Fundamentals and Practices of Reversed-Phase HPLC: Part II -- RPHPLC column configurations. It consists of the concept of separation, types of stationary and mobile phases with the variables concerned with the separation. This revi
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Zhang, Ping, Yuhan He, Sheng Wang, et al. "Chiral Separation and Determination of Etoxazole Enantiomers in Vegetables by Normal-Phase and Reverse-Phase High Performance Liquid Chromatography." Molecules 25, no. 14 (2020): 3134. http://dx.doi.org/10.3390/molecules25143134.

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The chiral separation of etoxazole enantiomers on Lux Cellulose-1, Lux Cellulose-3, Chiralpak IC, and Chiralpak AD chiral columns was carefully investigated by normal-phase high performance liquid chromatography and reverse-phase high performance liquid chromatography (HPLC). Hexane/isopropanol, hexane/n-butanol, methanol/water, and acetonitrile/water were used as mobile phase at a flow rate of 0.8 mL/min. The effects of chiral stationary phase, mobile phase component, mobile phase ratio, and temperature on etoxazole separation were also studied. Etoxazole enantiomers were baseline separated o
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Fogarty, Andrew M., Samuel J. Traina, and Olli H. Tuovinen. "Determination of Dicamba by Reverse-Phase HPLC." Journal of Liquid Chromatography 17, no. 12 (1994): 2667–74. http://dx.doi.org/10.1080/10826079408013406.

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Indira, S. Indira, K. Devi, and V. Kalvimoorthi. "Method Development and Validation of Luliconazole Bulk Drug Using Reverse Phase - HPLC Technique." Indian Journal Of Science And Technology 17, no. 39 (2024): 4094–100. http://dx.doi.org/10.17485/ijst/v17i39.2787.

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Objective: To develop and validate the RP- HPLC method for determining Luliconazole in bulk and pharmaceutical formulations. Methods: Following the International Conference on Harmonization (ICH) guidelines, a sensitive, accurate, and specific reversed-phase HPLC technique was created and validated for the detection of Luliconazole. Using high-performance liquid chromatography, this drug was examined. A better separation of the drug was achieved by using Agilent Eclipse XDB C (4.6mm x 250mm, 5µm) with a mobile phase consisting of a mixture of Acetonitrile and Buffer in HPLC water. The ratio of
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Pund, Swati, Sapnarani Nalawade, Vikas Rajurkar, Suresh Jayatpal, Nitin Deshmukh, and Harshal Tare. "A brief review on recent advances in reverse phase HPLC." Multidisciplinary Reviews 7, no. 4 (2024): 2024072. http://dx.doi.org/10.31893/multirev.2024072.

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Modern chromatography is mostly used for analytical purposes. Modern pharmaceutical and biological analysis uses HPLC. It separates molecules from heterogeneous liquids and characterizes them. Mass spectrometry identifies peptides, proteins, and other compounds after high-performance liquid chromatography. HPLC's flexibility and dependability (pressure-driven liquid support) make it popular. However, fast separation often creates high operating pressure, which stresses HPLC equipment. Thus, core-shell silica microspheres and zirconia packing have been produced recently. Fused-core or superfici
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Hauville, C., S. Rémita, P. Thérond, D. Jore, and M. Gardès-Albert. "Radiation induced peroxidation of polyunsaturated fatty acids: Recent results on formation of hydroperoxides." Canadian Journal of Physiology and Pharmacology 79, no. 2 (2001): 176–79. http://dx.doi.org/10.1139/y00-086.

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Aqueous solutions of linoleic acid were irradiated in air with γ-rays of 137Cs. High pressure liquid chromatography (HPLC) was been used to separate and measure the production of hydroperoxides. The results obtained after reverse phase chromatography, associated with a microperoxydase for hydroperoxide detection, indicate the presence of two different hydroperoxides. One type of hydroperoxide was the major product obtained when the initial linoleic concentrations were below the critical micellar concentration (2 mM), and the second type was produced when the concentrations were above 2 mM. A f
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Swamy, M. S., та E. C. Abraham. "Reverse-phase HPLC analysis of human α crystallin". Current Eye Research 10, № 3 (1991): 213–20. http://dx.doi.org/10.3109/02713689109003443.

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Dissertations / Theses on the topic "Reverse phase HPLC"

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Vo, Duyen Thuy. "Detection and Attempted Quantification of Allelopathic Chemicals in Buffelgrass (Pennisetum ciliare) Root Leachates Using Reverse Phase-HPLC." Thesis, The University of Arizona, 2013. http://hdl.handle.net/10150/297777.

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Pennisetum ciliare, commonly known as buffelgrass, is an invasive grass that has led to noticeable declines in perennial diversity and abundance. Allelopathy, which describes the exudation of secondary metabolites that result in detrimental effects upon neighboring species, has been implicated as a possible mechanism for buffelgrass' success due to observations that its root leachates inhibit the growth of native plants. Since past researchers have only revealed the identity of the acids present within the leachates (Table 1), I have proposed methods in which to extract and quantify these chem
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Favarin, Fernanda Reis. "PREPARAÇÃO, CARACTERIZAÇÃO E ATIVIDADE ANTIOXIDANTE DE LIPOSSOMAS CONTENDO ÁCIDO ASCÓRBICO." Centro Universitário Franciscano, 2017. http://www.tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/561.

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Submitted by MARCIA ROVADOSCHI (marciar@unifra.br) on 2018-08-17T19:52:35Z No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Dissertacao_FernandaReisFavarin.pdf: 2215176 bytes, checksum: f94c01cb7f3e20eec81ca0cf6c5bf3bd (MD5)<br>Made available in DSpace on 2018-08-17T19:52:35Z (GMT). No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Dissertacao_FernandaReisFavarin.pdf: 2215176 bytes, checksum: f94c01cb7f3e20eec81ca0cf6c5bf3bd (MD5) Previous issue date: 2017-05-11<br>Ascorbic acid (AA) is a water soluble vitam
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Watson, Richard Charles. "Studies of reversed phase high performance liquid chromatography (RP-HPLC) stationary phases." Thesis, University of Nottingham, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.338492.

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Chlup, Markus. "Methodenentwicklungen in der zweidimensionalen Reversed-Phase Hochleistungsflüssigkeitschromatographie." [S.l. : s.n.], 2008. http://nbn-resolving.de/urn:nbn:de:bsz:289-vts-66329.

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Essaka, David Christian. "Reversed-Phase HPLC Determination of Cholesterol in Food Items." Digital Commons @ East Tennessee State University, 2007. https://dc.etsu.edu/etd/2034.

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Cholesterol is a fat-like molecule found among lipids in animal (including human) tissues. It is needed for maintaining good health. However, health issues have been raised because of the strong correlation between high levels of cholesterol in the body and cardiovascular disease. An HPLC method for quantitative determination of cholesterol in foods is presented. This involves a C-18 stationary phase using a 70:30 methanol: 2-propanol mobile phase with an UV detector set at 212 nm. The method showed linearity in the range 5.0 to 100.0 μg/mL and also good reproducibility with relative standard
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Aries, R. E. "Some technological aspects of reversed phase narrow bore HPLC columns." Thesis, University of Reading, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.373123.

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Bartuma, Ninorta. "Optimizing purification of oligonucleotides with reversed phase trityl-on solid phase extraction." Thesis, Örebro universitet, Institutionen för naturvetenskap och teknik, 2019. http://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-76844.

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Oligonucleotides are synthetic strings of DNA or RNA used mostly for biochemical analysis and diagnostics. For them to be useful in these fields, a purity over 90% is most often required. However, when synthesizing these sequences, many “failures” (shorter sequences) are made in the step-wise process. The synthesized oligonucleotides need to therefore be purified. This is most often done with gel electrophoresis or liquid chromatography. These methods are, on the other hand, very time-consuming and laborious. Solid phase extraction (SPE) is a much faster purification method if optimized and it
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Patel, Hasmukh B. "Reversed-phase high-performance liquid chromatography : some basic studies and application in microbial transformation." Thesis, University of Bath, 1987. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.379158.

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Sexton, Danessa Leann. "Determination of lactose by reversed-phase high performance liquid chromatography." [Johnson City, Tenn. : East Tennessee State University], 2004. http://etd-submit.etsu.edu/etd/theses/available/etd-0328104-212023/unrestricted/SextonD041504f.pdf.

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Thesis (M.S.)--East Tennessee State University, 2004.<br>Title from electronic submission form. ETSU ETD database URN: etd-0328104-212023. Includes bibliographical references. Also available via Internet at the UMI web site.
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AL-Shaer, Mahmoud. "Reversed-Phase HPLC Determination of Citral in Locally Grown Lemon Grass Harvested at Different Season." Digital Commons @ East Tennessee State University, 2006. https://dc.etsu.edu/etd/2229.

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A simple HPLC procedure for the quantitative determination of citral, the major fragrant component in the lemon grass, has been developed. The procedure involves a C-8 stationary phase using a 90:10 methanol: water pH 5 mobile phase containing 0.25% 1-octanesulfonic acid and an UV detector (set at 233 nm). The lemon grass leaves were harvested fresh at different times of the year and were soaked in methanol for 48 hours without any mechanical assistance to extract the citral and other methanol soluble components. The method showed good reproducibility with relative standard deviation of 2.8% a
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Books on the topic "Reverse phase HPLC"

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Szepesi, Gábor. How to use reverse-phase HPLC. VCH Publishers, 1992.

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F, Jenkins T., and Cold Regions Research and Engineering Laboratory (U.S.), eds. Reverse phase HPLC method for analysis of TNT, RDX, HMX and 2,4-DNT in munitions wastewater. US Army Corps of Engineers, Cold Regions Research & Engineering Laboratory, 1985.

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FEDERATION, INTERNATIONAL DAIRY. IDF Standard 178A: 1999 Milk and heat-treated milk: determination of acis soluble b-lactoglobulin content: reversed-phase hplc method. International Dairy Federation, 1997.

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Szepesi, Gabor. How to Reverse-phase HPLC. VCH Verlagsgesellschaft,Germany, 1992.

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Szepesi, G. How to Use Reverse-Phase HPLC. Wiley & Sons, Incorporated, John, 1992.

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Adam Ashton Kinion M S. Reverse Phase HPFC Protocols & Notes: High Performance Liquid Chromatography. Independently Published, 2019.

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Lork, Klaus-Dieter Heinz. Einfluss der Eigenschaften von Reversed Phase Trägern auf ihr Retentionsverhalten in der HPLC. 1988.

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IDF standard 178: 1996. Milk and heat-treated milk, determination of acid soluble B-Lactoglobulin content, reversed-phase HPLC method. International Dairy Federation, 1996.

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Book chapters on the topic "Reverse phase HPLC"

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Shively, John E. "Reverse-Phase HPLC Isolation and Microsequence Analysis." In Methods of Protein Microcharacterization. Humana Press, 1986. http://dx.doi.org/10.1007/978-1-59259-436-8_2.

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Stone, Kathryn L., and Kenneth R. Williams. "Reverse-Phase HPLC Separation of Enzymatic Digests of Proteins." In Springer Protocols Handbooks. Humana Press, 1996. http://dx.doi.org/10.1007/978-1-60327-259-9_72.

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Stone, Kathryn L., and Kenneth R. Williams. "Reverse-Phase HPLC Separation of Enzymatic Digests of Proteins." In Springer Protocols Handbooks. Humana Press, 2009. http://dx.doi.org/10.1007/978-1-59745-198-7_102.

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Amantea, Gerry F., Vesna N. Furtula, Ho Yeon Choi, Louis C. Laleye, and Shuryo Nakai. "Assessment of Accelerated Cheese Ripening by Reverse-Phase HPLC." In Chemistry of Structure-Function Relationships in Cheese. Springer US, 1995. http://dx.doi.org/10.1007/978-1-4615-1913-3_8.

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Dagenais, Pierre, and Pascale Legault. "Preparative Separation of Ribonucleoside Monophosphates by Ion-Pair Reverse-Phase HPLC." In Recombinant and In Vitro RNA Synthesis. Humana Press, 2012. http://dx.doi.org/10.1007/978-1-62703-113-4_18.

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Jones, Barry Nelson. "Amino Acid Analysis by o-Phthaldialdehyde Precolumn Derivatization and Reverse-Phase HPLC." In Methods of Protein Microcharacterization. Humana Press, 1986. http://dx.doi.org/10.1007/978-1-59259-436-8_5.

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Gruber, Stephen, Noel M. Meltzer, Stanley Stein, and Guillermo I. Tous. "An Improved Reverse Phase HPLC Separation and Postcolumn Detection of Amino Acids." In Proteins. Springer US, 1987. http://dx.doi.org/10.1007/978-1-4613-1787-6_23.

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Spoto, Giuseppe, Sandro Berardi, Giuseppe Ajerba, and Vittorio De Laurentiis. "A Reverse-Phase HPLC Method for Cyclic Nucleotide Phosphodiesterases Activity and Classification." In Purine and Pyrimidine Metabolism in Man VIII. Springer US, 1995. http://dx.doi.org/10.1007/978-1-4615-2584-4_171.

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Yan, Chong Chao, and Ryan J. Huxtable. "Determination of Cysteinyl-Containing Peptides and Associated Enzyme Activities in Rat Tissues by Reverse Phase HPLC." In Advances in Experimental Medicine and Biology. Springer US, 1998. http://dx.doi.org/10.1007/978-1-4899-0117-0_6.

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LoBrutto, Rosario, and Yuri Kazakevich. "Reversed-Phase HPLC." In HPLC for Pharmaceutical Scientists. John Wiley & Sons, Inc., 2006. http://dx.doi.org/10.1002/9780470087954.ch4.

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Conference papers on the topic "Reverse phase HPLC"

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Balamurugan, P., K. Anver Basha, and P. Parthiban. "Short reverse phase HPLC method for 5-methoxy-2-mercaptobenzimidazole related substance estimation." In 2014 International Conference on Science Engineering and Management Research (ICSEMR). IEEE, 2014. http://dx.doi.org/10.1109/icsemr.2014.7043615.

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Zhou, Ran, Ming Chang, Fei Wang, and Ruisheng Hu. "Determination of related substance of cefotiam hydrochloride for injection using reverse-phase HPLC methods." In International Conference on Medical Engineering and Bioinformatics. WIT Press, 2014. http://dx.doi.org/10.2495/meb140571.

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AlOudah, Yahya. "Waste Management Control by Applying Novel Method for HPLC Technique to Replace SKALAR Cadmium Reduction Method for Nitrate and Nitrite in Water Analysis." In International Petroleum Technology Conference. IPTC, 2022. http://dx.doi.org/10.2523/iptc-22291-ea.

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Abstract A new method was developed on High Performance Liquid Chromatography (HPLC) instrument equipped with a Photodiode Array UV detector to determine the Nitrite-Nitrogen and Nitrate-Nitrogen in seawater, industrial wastewater, and groundwater at very low concentrations. This HPLC used a reverse phase system with polar mobile phase and nonpolar C-18 Column. The split mechanism of the HPLC Chromatography was accomplished by employing acetonitrile and acidified water with pH 2.5, at 55:45 ACN: H2O v/v as a mobile phase, long HPLC Column (C-18, 0.5 μm, 250 × 4.6 mm) and at 1 mL/min flow rate.
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Bauer, K. A., B. L. Kass, M. Bednarek, M. Kloczewiak, J. Hawiger, and R. D. Rosenberg. "DETECTION OF FACTOR X ACTIVATION IN HUMANS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643828.

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The activation of factor X by factor VII/VIIa-tissue factor or factor IXa plays a pivotal role in the hemostatic mechanism. This reaction results in the liberation of a peptide from the zymogen for which we have developed a sensitive and specific radioimmunoassay (RIA), The native peptide was purified from activated human factor X by hydroxylapatite chromatography and reverse-phase high pressure liquid chromatography (HPLC). Gel filtration experiments demonstrated that the peptide was not physically associated with the enzyme. A 15 amino acid peptide with the COOH-terminal sequence of the acti
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Lund-Hansen, T., and L. C. Peterson. "COMPARISON OF ENZYMATIC PROPERTIES OF HUMAN PLASMA FVIIa AND HUMAN RECOMBINANT FVIIa." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643787.

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Human plasma FVIIa (pFVIIa) and human recombinant FVIIa (rFVIIa) were both purified by immune adsorption chromatography using a calcium dependent monoclonal antibody. The FVII obtained is highly purified and contains only trace contaminants as revealed by SDS-PAGE and reverse phase HPLC chromatography. FVII was fully activated during the purification procedure. A FVIIa activity assay has been developed in microplates using human FX as a substrate and methoxycarbonyl-D-cyclohexal-alanyl-glycyl-arginine-pNA as a chromogenic substrate for the FXa generated. The assay was linear at FVIIa concentra
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Varela, D., R. O’Hara, and A. C. Neves. "BY-PRODUCTS OF THE WHELK PROCESSING INDUSTRY AS VALUABLE SOURCE OF ANTIOXIDANT PEPTIDES." In World Conference on Waste Management. The International Institute of Knowledge Management, 2021. http://dx.doi.org/10.17501/26510251.2021.1103.

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The fish and shellfish industry processes 851,984 tonnes of fish per year worldwide. However, only 43% of that is consumed, and valuable proteins are processed as waste. Protein hydrolysates are widely used in food technology for their nutritional and functional properties. The goal of this project is to extract protein from whelk by-products derived from the shellfish processing industry and create protein hydrolysates that have marketable value. The by-products were divided into two types: raw (R) and cooked byproduct (C). The proteins were extracted using the pH shift method and quantified
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Stanić, Petar B., Biljana Šmit, Jovana Muškinja, et al. "Normal and Reversed Phases Thin-Layer Chromatography of Arylidene 2- Thiohydantoin Derivatives." In 2nd International Conference on Chemo and Bioinformatics. Institute for Information Technologies, University of Kragujevac, 2023. http://dx.doi.org/10.46793/iccbi23.531s.

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In this paper, the retention behavior of thirteen newly synthesized arylidene derivatives of 2-hydamthione was investigated by normal and reversed phases thin-layer chromatography. In normal phase chromatography, thin layers of silica gel and cyano-propyl (CN) silica gel with fluorescent indicator F254 were used as the stationary phase. As the mobile phase, binary systems of non-aqueous solvents benzene-ethyl acetate and hexane-ethyl acetate were used, 8:2 (v/v). Reversed phase chromatography was performed using a thin layer of octadecyl (C-18) silica gel with fluorescent indicator F254 as the
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Rabiet, M. J., B. C. Furie, and B. Furie. "MOLECULAR DEFECT IN PROTHROMBIN MADRID: SUBSTITUTION OF ARGININE 273 BY CYSTEINE PRECLUDES ACTIVATION." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643936.

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Prothrombin Madrid, a mutant prothrombin, was detected in a patient with a excessive bleeding history. The defect was characterized by a low coagulant activity contrasting with a normal level of prothrombin antigen in plasma. Activation of the purified protein was impaired by the absence of one of the two factor Xa catalyzed cleavages, generating meizothrombin which expressed a thrombin-like activity but was inactive on fibrinogen (Guillin et al., Ann. N.Y. Acad. Sci. 370:414, 1981). Prothrombin and prothrombin Madrid were isolated directly from plasma, with high yield, by immunoaffinity chrom
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Terukina, S., M. Matsuda, N. Yoshida, K. Yamazumi, Y. Takeda та T. Takano. "TWO ABNORMAL FIBRINOGENS DESIGNATED AS OSAKA II AND MORIOKA WITH A HITHERTO UNIDENTIFIED AMINO ACID SUBSTITUTION; γARG-275 BY CYS". У XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644701.

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A hitherto unidentified amino acid substitution of γ Arg-275 by Cys has been found in two abnormal fibrinogens, Osaka II and Morioka. The propositi are both asymptomatic heterozygotes for the abnormality characterized by altered polymerization of fibrin monomers. Reducing SDS-PAGE revealed that fibrinogens derived from thé propositi both consist of two populations; one with a normal and the other with an abnormal longer γ-chain by 0.5 Kd.The γ-γ cross-linking took place nearly normally, however. Analyzing plasmic digests of fibrinogen by SDS-PAGE, we located the abnormality residing in the γ-c
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Purdon, A. D., and J. B. Smith. "RELEASE AND TRANSACYLATION OF ARACHIDONATE FROM A COMMON POOL OF 1-ACYL-2-ARACHIDONOYL GLYCEROPHOSPHOCHOLINE IN HUMAN PLATELETS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643391.

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We have previously shown that the main source of arachidonate in thrombin-stimulated human platelets is 1-acyl-2-arachidonoyl (AA) glycerophosphocholine (GPC) and release of 3H-AA from this phospholipid also was correlated with increased 3H-AA in ether phospholipid. This ATP independent transfer of 3H-AA from 1,2 diacyl GPC to ether phospholipid (transacylation) also occurs in resting cells. Human platelets in 1/10 volume of plasma (ACD anticoagulant, pH 6.5) were radiolabelled with 3H-AA for 60 min at 37°C and then exogenous 3H-AA was removed by gel filtration into Tyrode's buffer, pH 7.4, 0.
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Reports on the topic "Reverse phase HPLC"

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Annunziato, Dom. High-Performance Liquid Chromatography Analysis of Psilocybin-Containing Mushrooms: Key Considerations and Insights. GenTech Scientific LLC, 2023. http://dx.doi.org/10.61073/gentech.hplc.top.5.

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HPLC analysis of psilocybin-containing mushrooms is a complex process that requires careful attention to detail. In this scientific summary, Doma Nunzio1, MagicMyco C.S.O., offers insight and new answers to some old questions about these magic fungi. Based on his dedicated research and progress in the field, here are his top five things to keep in mind when conducting HPLC-UV analysis in reverse phase ‘RP Mode’ for psilocybin-containing mushrooms.
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พงษ์สวัสดิ์, เปี่ยมสุข, та ทิพาพร ลิมปเสนีย์. ลักษณะโครงสร้างของไซโคลเดกซ์ทรินไกลโคซิลทรานสเฟอเรสไอโซไซม์จาก Bacillus circulans A11 : รายงานผลการวิจัย. จุฬาลงกรณ์มหาวิทยาลัย, 2002. https://doi.org/10.58837/chula.res.2002.35.

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ศึกษาลักษณะโครงสร้างของไซโคลเดกซ์ทรินไกลโคซิลทรานสเฟอเรส (CGTase) ไอโซไซม์ จาก Bacillus circulans A11 โดยวิเคราะห์บริเวณเร่งตรวจสอบกรดอะมิโนและเพปไทด์ที่เป็นองค์ประกอบ รวมทั้งการศึกษาผลของไกลโคซิเลชันต่อแอคติวิตีและโครงสร้างของไอโซไซม์ เพื่อนำข้อมูลมาประกอบการวิเคราะห์การเกิดหลายรูปแบบของเอนไซม์ ในขั้นตอนแรกได้แยกไอโซไซม์ทั้ง 4 ของ CGTase ด้วยเทคนิคเจลอิเล็กโทรโฟริซิสแบบ preparative แล้วบ่มแต่ละไอโซไซม์ด้วยสารเคมีที่จำเพาะต่อหมู่ฟังก์ชันเพื่อดัดแปรกรดอะมิโน และใช้เทคนิคการป้องกันบริเวณเร่งด้วยสับสเตรทเมทธิล-บีด้าไซโคลเดกซ์ทรินก่อนการดัดแปร ผลการทดลองพบว่า กรดอะมิโนที่อยู่ในบริเวณเร่ง และมีความ
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Peter W. Carr, K.M. Fuller, D.R. Stoll, L.D. Steinkraus, M.S. Pasha, and Glenn G. Hardin. Fast Gradient Elution Reversed-Phase HPLC with Diode-Array Detection as a High Throughput Screening Method for Drugs of Abuse. Office of Scientific and Technical Information (OSTI), 2005. http://dx.doi.org/10.2172/892807.

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