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Relevant bibliographies by topics / Reverse phase HPLC / Dissertations / Theses

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Dissertations / Theses on the topic 'Reverse phase HPLC'

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Published: 3 June 2025

Last updated: 31 July 2025

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1

Vo, Duyen Thuy. "Detection and Attempted Quantification of Allelopathic Chemicals in Buffelgrass (Pennisetum ciliare) Root Leachates Using Reverse Phase-HPLC." Thesis, The University of Arizona, 2013. http://hdl.handle.net/10150/297777.

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Pennisetum ciliare, commonly known as buffelgrass, is an invasive grass that has led to noticeable declines in perennial diversity and abundance. Allelopathy, which describes the exudation of secondary metabolites that result in detrimental effects upon neighboring species, has been implicated as a possible mechanism for buffelgrass' success due to observations that its root leachates inhibit the growth of native plants. Since past researchers have only revealed the identity of the acids present within the leachates (Table 1), I have proposed methods in which to extract and quantify these chem

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2

Favarin, Fernanda Reis. "PREPARAÇÃO, CARACTERIZAÇÃO E ATIVIDADE ANTIOXIDANTE DE LIPOSSOMAS CONTENDO ÁCIDO ASCÓRBICO." Centro Universitário Franciscano, 2017. http://www.tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/561.

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Submitted by MARCIA ROVADOSCHI (marciar@unifra.br) on 2018-08-17T19:52:35Z No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Dissertacao_FernandaReisFavarin.pdf: 2215176 bytes, checksum: f94c01cb7f3e20eec81ca0cf6c5bf3bd (MD5)<br>Made available in DSpace on 2018-08-17T19:52:35Z (GMT). No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Dissertacao_FernandaReisFavarin.pdf: 2215176 bytes, checksum: f94c01cb7f3e20eec81ca0cf6c5bf3bd (MD5) Previous issue date: 2017-05-11<br>Ascorbic acid (AA) is a water soluble vitam

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3

Watson, Richard Charles. "Studies of reversed phase high performance liquid chromatography (RP-HPLC) stationary phases." Thesis, University of Nottingham, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.338492.

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4

Chlup, Markus. "Methodenentwicklungen in der zweidimensionalen Reversed-Phase Hochleistungsflüssigkeitschromatographie." [S.l. : s.n.], 2008. http://nbn-resolving.de/urn:nbn:de:bsz:289-vts-66329.

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5

Essaka, David Christian. "Reversed-Phase HPLC Determination of Cholesterol in Food Items." Digital Commons @ East Tennessee State University, 2007. https://dc.etsu.edu/etd/2034.

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Cholesterol is a fat-like molecule found among lipids in animal (including human) tissues. It is needed for maintaining good health. However, health issues have been raised because of the strong correlation between high levels of cholesterol in the body and cardiovascular disease. An HPLC method for quantitative determination of cholesterol in foods is presented. This involves a C-18 stationary phase using a 70:30 methanol: 2-propanol mobile phase with an UV detector set at 212 nm. The method showed linearity in the range 5.0 to 100.0 μg/mL and also good reproducibility with relative standard

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6

Aries, R. E. "Some technological aspects of reversed phase narrow bore HPLC columns." Thesis, University of Reading, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.373123.

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7

Bartuma, Ninorta. "Optimizing purification of oligonucleotides with reversed phase trityl-on solid phase extraction." Thesis, Örebro universitet, Institutionen för naturvetenskap och teknik, 2019. http://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-76844.

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Oligonucleotides are synthetic strings of DNA or RNA used mostly for biochemical analysis and diagnostics. For them to be useful in these fields, a purity over 90% is most often required. However, when synthesizing these sequences, many “failures” (shorter sequences) are made in the step-wise process. The synthesized oligonucleotides need to therefore be purified. This is most often done with gel electrophoresis or liquid chromatography. These methods are, on the other hand, very time-consuming and laborious. Solid phase extraction (SPE) is a much faster purification method if optimized and it

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8

Patel, Hasmukh B. "Reversed-phase high-performance liquid chromatography : some basic studies and application in microbial transformation." Thesis, University of Bath, 1987. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.379158.

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9

Sexton, Danessa Leann. "Determination of lactose by reversed-phase high performance liquid chromatography." [Johnson City, Tenn. : East Tennessee State University], 2004. http://etd-submit.etsu.edu/etd/theses/available/etd-0328104-212023/unrestricted/SextonD041504f.pdf.

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Thesis (M.S.)--East Tennessee State University, 2004.<br>Title from electronic submission form. ETSU ETD database URN: etd-0328104-212023. Includes bibliographical references. Also available via Internet at the UMI web site.

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10

AL-Shaer, Mahmoud. "Reversed-Phase HPLC Determination of Citral in Locally Grown Lemon Grass Harvested at Different Season." Digital Commons @ East Tennessee State University, 2006. https://dc.etsu.edu/etd/2229.

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A simple HPLC procedure for the quantitative determination of citral, the major fragrant component in the lemon grass, has been developed. The procedure involves a C-8 stationary phase using a 90:10 methanol: water pH 5 mobile phase containing 0.25% 1-octanesulfonic acid and an UV detector (set at 233 nm). The lemon grass leaves were harvested fresh at different times of the year and were soaked in methanol for 48 hours without any mechanical assistance to extract the citral and other methanol soluble components. The method showed good reproducibility with relative standard deviation of 2.8% a

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11

Davies, Nicola Helen. "Effects of pH and column chemistry on the overload of basic compounds in reversed-phase HPLC." Thesis, University of the West of England, Bristol, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.444502.

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12

Apawu, Aaron Kwaku. "Reversed-Phase HPLC Determination of Alliin in Diverse Varieties of Fresh Garlic and Commercial Garlic Products." Digital Commons @ East Tennessee State University, 2009. https://dc.etsu.edu/etd/1803.

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Alliin is a predominant flavor precursor in garlic cloves. It interacts with the enzyme alliinase when garlic cloves are crushed, cut, or chewed to produce allicin, an unstable thiosulfinate that is the main biologically active component of fresh crushed garlic. Biological functions and health benefits of garlic include reduction of cancer risk in humans, improving immune system, and anti-microbial, anti-oxidant, and anti-hypertensive activities. The quality of fresh garlic and garlic products is usually related to its alliin content and allicin release potential. This research presents a s

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13

Nome, Renata Cristiano 1975. "Preparação de fases estacionárias para cromatografia líquida de alta eficiência em fase reversa a partir da imobilização térmica do poli(metiltetradecilsiloxano) sobre sílica aluminizada." [s.n.], 2014. http://repositorio.unicamp.br/jspui/handle/REPOSIP/249932.

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Orientador: Carol Hollingworth Collins<br>Tese (doutorado) - Universidade Estadual de Campinas, Instituto de Química<br>Made available in DSpace on 2018-08-26T17:56:06Z (GMT). No. of bitstreams: 1 Nome_RenataCristiano_D.pdf: 3193413 bytes, checksum: 499dd8e787afa50c91a2295212001ca4 (MD5) Previous issue date: 2014<br>Resumo: Este trabalho apresenta o desenvolvimento de fases estacionárias (FE) para utilização em Cromatografia Líquida em Alta Eficiência em Fase Reversa (CLAE-FR) a partir da sorção e imobilização de poli(metiltetradecilsiloxano) (PMTDS) sobre suportes de sílica metalizada com a

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14

Murphy, Kellyann M. "Analysis of Biodiesel Quality Using Reversed Phase High-Performance Liquid Chromatography." Scholarship @ Claremont, 2012. http://scholarship.claremont.edu/pomona_theses/45.

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The alternative fuel biodiesel is produced from the transesterification of vegetable oils or animal fat to fatty acid methyl esters. Pomona has a reactor on campus that can be used to run this reaction and produce biodiesel. The use of biodiesel has been found to lower air pollutant and greenhouse gas emissions, but can be potentially harmful to the engines if it contains impurities. This paper proposes a method using high-performance liquid chromatography to test the quality of biodiesel. This method utilizes instrumentation and materials that are available in Pomona College's Chemistry Depar

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15

Shao, Peimin. "SFE Fractionation and RP-HPLC Characterization of Aquatic Fulvic Acid." Thesis, University of North Texas, 1994. https://digital.library.unt.edu/ark:/67531/metadc500352/.

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The Supercritical Fluid Extraction (SFE) technique was used to fractionate Suwannee River reference fulvic acid (FA). The fractions were characterized by gas chromatography (GC) and reversed-phase high performance liquid chromatography (RP-HPLC). A SFE fractionation method was developed using stepwise gradient of supercritical CO₂ and methanol. Three FA fractions were separated. The average mass recovery was 102% with the coefficient of variation of 2.8%. The fractionation dynamics and the difference in the ratios of UV absorption to fluorescence emission indicate the real fractionation of the

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16

Figueiredo, Junior José Wilson. "Avaliação da utilidade de um software de simulação cromatográfica na separação de Flavonóides por CLAE-DAD de fase reversa." Universidade Federal da Paraíba, 2008. http://tede.biblioteca.ufpb.br:8080/handle/tede/7253.

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Made available in DSpace on 2015-05-14T13:24:23Z (GMT). No. of bitstreams: 1 arquivototal.pdf: 2292715 bytes, checksum: cb27e9ba3f75b8d32163a1ae715a147c (MD5) Previous issue date: 2008-05-27<br>Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES<br>High Performance Liquid Chromatography (HPLC) allows both qualitative and quantitative chemical analysis, separating and identifying substances in mixtures with speed and efficiency. However, factors governing chromatographic retention are complex and the method development phase consumes a lot of resources. In our work we eva

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17

Liang, Hongxi. "An investigation into the use of #beta#-cyclodextrins as additives to effect enantiometric separation by reversed phase HPLC." Thesis, Robert Gordon University, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.260042.

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18

Collins, Matthew P. "Reversed Phase Liquid Chromatography Using the Ionic Liquid Isopropylammonium Formate and Comparison of Indirect Spectrophotometric Methods for Phosphate." Miami University / OhioLINK, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=miami1312342055.

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19

Henriksson, Emma, and Martin Holm. "Kvantitativ analys av organiska syror i kylvätska." Thesis, Högskolan i Borås, Akademin för textil, teknik och ekonomi, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:hb:diva-13499.

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Volvo Cars är ett välkänt företag som säljer bilar över hela världen. Idag skickar Volvo Cars sin kylvätska till leverantörer för analys. Processen är komplicerad och tar mycket tid. Det finns ett intresse hos Volvo Cars att själva kunna analysera kylvätskan. För att kunna göra detta krävs det att en metod som de kan använda för att analysera kylvätskan utvecklas. Examensarbetet syftar till att utveckla en metod som gör det möjligt att analysera koncentrationen av organiska syror i Volvos kylvätska. Analysen ska göras med hjälp av en High Performance Liquid Chromatography - HPLC. De organiska

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20

Iglebaek, Herceglija Ena. "Stability testing of all-trans-retinol in an experimental cosmetic formulation and in methanol and methanol containing butylhydroxytoluene (BHT) using reversed-phase HPLC." Thesis, KTH, Kemi, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-302695.

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All-trans-retinol (vitamin A) används ofta som en aktiv ingrediens i kosmetiska produkter på grund av dess kliniskt validerade anti-age-egenskaper. Däremot är användningen av all-trans-retinol i kosmetiska formuleringar begränsad till följd av den kemiska stabiliteten av all-trans-retinol som påverkas av värme, syre, ljus och syror. Både kompositionen av en kosmetisk formulering, samt lagringsförhållanden och förpackningen av produkten, kan påverka nedbrytningen av all-trans-retinol till mindre aktiva former. I denna studie har stabilitetstestning genomförts under två månader i syfte att utvä

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21

Pedada, Kiran K. Mr. "GRADIENT CHROMATOFOCUSING AND REVERSED PHASE HPLC IN PROTEIN ANALYSIS AND LC-MS/MS DETERMINATION OF THIAZOLIDINEDIONE NL-1 IN MOUSE SERUM AND BRAIN." Cleveland State University / OhioLINK, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=csu1390322545.

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22

Alshana, Usama Ahmed. "Separation And Quantitation Of Some Platinum Group Metals By Rp-hplc." Master's thesis, METU, 2005. http://etd.lib.metu.edu.tr/upload/3/12605760/index.pdf.

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In this study, a reversed-phase high performance liquid chromatography (RP-HPLC) method has been developed to separate and determine Pt and Pd after formation of their chelates with N,N-diethyl-N&#039<br>-benzoylthiourea (DEBT). With the aim of reducing the number of steps in treating the samples, the method developed does not require the elimination of excess chelating reagent before the analysis of metal chelates. The different physical and chemical parameters affecting separation were examined in details. The whole analysis was completed on a C18 column in 16 min at 280 nm, with the mobile

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23

Undin, Torgny. "Development and improvement of methods for characterization of HPLC stationary phases." Licentiate thesis, Uppsala universitet, Analytisk kemi, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-166978.

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High Performance Liquid Chromatography (HPLC) is a widely used tech-nique both for detecting and purifying substances in academy and in the industry. In order to facilitate the use of, and knowledge in HPLC, character-ization of stationary phases is of utmost importance. Tailor made characteri-zation methods and workflows are steadily increasing the speed and accura-cy in which new separation systems and methods are developed. In the field fundamental separation science and of preparative chromatography there is always the need for faster and more accurate methods of adsorption isotherm determ

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24

Chiaradia, Mariza Campagnolli. "Fases estacionarias reversas a base de silica aluminizada, com poli(metiloctilsiloxano) imobilizado termicamente." [s.n.], 2005. http://repositorio.unicamp.br/jspui/handle/REPOSIP/249913.

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Orientador: Carol Hollingworth Collins<br>Dissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Quimica<br>Made available in DSpace on 2018-08-05T10:44:35Z (GMT). No. of bitstreams: 1 Chiaradia_MarizaCampagnolli_M.pdf: 950339 bytes, checksum: 7214a72f3a4f6801202145112446bc30 (MD5) Previous issue date: 2005<br>Resumo: Neste trabalho foram preparadas e avaliadas fases estacionárias (FE) utilizando sílica modificada com alumina como suporte e poli(metiloctilsiloxano) (PMOS) imobilizado por tratamento térmico em sua superfície, com o objetivo de se obter fases estáveis e efici

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25

Magalhães, Daniel Rodrigues. "Desenvolvimento de fases estacionarias de polaridade intermediaria para cromatografia liquida de alta eficiencia em fase reversa." [s.n.], 2005. http://repositorio.unicamp.br/jspui/handle/REPOSIP/249930.

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Orientador: Carol Hollingworth Collins<br>Tese (doutorado) - Universidade Estadual de Campinas, Instituto de Quimica<br>Made available in DSpace on 2018-08-05T16:30:01Z (GMT). No. of bitstreams: 1 Magalhaes_DanielRodrigues_D.pdf: 3095259 bytes, checksum: 34caab6a50b8a2f3071fca69103ab15f (MD5) Previous issue date: 2005<br>Doutorado<br>Quimica Analitica<br>Doutor em Ciências

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Garcia, Ausberta Jesús Cabezas. "Metodologia analítica para determinação de triclosan e clorofenois por cromatografia a líquido de alta eficiência (HPLC) e cromatografia por injeção seqüencial (SIC) com uso de coluna monolítica e empacotada." Universidade de São Paulo, 2011. http://www.teses.usp.br/teses/disponiveis/46/46136/tde-28032012-112652/.

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Foram desenvolvidas metodologias de cromatografia a líquido de fase reversa baseadas em injeção sequencial (SIC) e em cromatografia a líquido de alta eficiência (HPLC) para determinação de triclosan em amostras de produtos de higiene pessoal e em estudos de adsorção em argilominerais naturais e modificados, visando determinar parâmetros de adsorção de triclosan frente a alguns de seus metabólitos. A determinação de triclosan em enxaguadores bucais foi realizada por SIC com eluição isocrática usando fase móvel constituída por acetonitrila: tampão fosfato de trietilamina 70 mM pH 3,5 na proporçã

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27

Mautjana, Nare Alpheus. "Determination of platinum, palladium and rhodium, in aqueous media by means of reversed phase-high performance liquid chromatography (RP-HPLC) after complexation with N,N-dialkyl-N'-acylthioureas." Master's thesis, University of Cape Town, 2000. http://hdl.handle.net/11427/11809.

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Includes bibliographical references.<br>The complexes were successfully separated using conventional Reversed Phase-High Performance Liquid Chromatography (RP-HPLC) and a UV detector. A simple method of complexation of platinum(II) and palladium(II) in acidic, aqueous samples was developed. This method involves the mixing of excess ligand (HL") in acetonitrile with aqueous solutions containing traces of platinum(II) and palladium(II) at room temperature followed by salt-induced phase separation prior to RP-HPLC analysis. In the case of rhodium(III), however, complexation was extremely slow and

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Maldaner, Liane. "Fases estacionarias de poli(metil-3,3,3-trifluorpropilsiloxano) imobilizadas sobre suportes de silica para cromatografia liquida de alta eficiencia em fase reversa = preparo e caracterização." [s.n.], 2010. http://repositorio.unicamp.br/jspui/handle/REPOSIP/250536.

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Orientador: Isabel Cristina Sales Fontes Jardim<br>Tese (doutorado) - Universidade Estadual de Campinas, Instituto de Quimica<br>Made available in DSpace on 2018-08-15T06:33:14Z (GMT). No. of bitstreams: 1 Maldaner_Liane_D.pdf: 4323260 bytes, checksum: 773bada382fd206c910361612918f566 (MD5) Previous issue date: 2010<br>Resumo: Novas fases estacionárias (FE) fluoradas foram preparadas a partir da imobilização do poli(metil-3,3,3-trifluorpropilsiloxano) (PMTFS) em partículas de sílica Kromasil de 5 mm, por tratamento termico (TT) ou por radiação micro-ondas (RM). As condições de imobilização o

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29

Wijekoon, Asanka. "Preparation and Characterization of Multifunctional Stationary Phases for Multimode Separations." Kent State University / OhioLINK, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=kent1263945054.

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Loureiro, Renan Fernandes. "Caracterização físico-química da foliculotrofina humana (hFSH) recombinante e de suas subunidades, por cromatografia líquida de alta eficiência (HPLC) em fase reversa: comparação com a preparação de referência de hFSH de origem hipofisária do \"National Hormone and Pituitary Program\" dos EUA." Universidade de São Paulo, 2006. http://www.teses.usp.br/teses/disponiveis/85/85131/tde-30032012-100440/.

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Um método de cromatografia líquida de alta eficiência por fase reversa (RP-HPLC) para análise qualitativa e quantitativa do hormônio folículo estimulante humano íntegro (hFSH), foi estabelecido e validado quanto à exatidão, precisão e sensibilidade. O FSH humano é um hormônio glicoprotéico dimérico largamente utilizado em medicina reprodutiva tanto para diagnóstico quanto para terapia. A metodologia desenvolvida preserva a integridade da proteína, permitindo a análise da forma heterodimérica intacta, e não somente de suas subunidades, como é normalmente obtida na maioria das condições geralmen

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Delrieu, Pascal. "Utilisation d'acides perfluores en clhp de phase inverse : application a la purification semi-preparative de peptides et d'antibiotiques." Toulouse 3, 1988. http://www.theses.fr/1988TOU30039.

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Van, Meter David S. III. "Synthesis and Characterization of Surface-Confined Ionic Liquid Stationary Phases for High Performance Liquid Chromatography." University of Cincinnati / OhioLINK, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1227275073.

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Nkabyo, Henry Ane. "A study on the reversible photo-induced isomerisation of platinum(II) and palladium(II) complexes of the N,N-dialkyl-N’-acyl(aroyl)thioureas with reversed-phase HPLC separation from related rhodium(III), ruthenium(III) and iridium(III) complexes." Thesis, Stellenbosch : Stellenbosch University, 2014. http://hdl.handle.net/10019.1/86773.

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Edström, Lena. "Adsorption Studies with Liquid Chromatography : Experimental Preparations for Thorough Determination of Adsorption Data." Doctoral thesis, Uppsala universitet, Analytisk kemi, 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-216235.

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Analytical chemistry is a field with a vast variety of applications. A robust companion in the field is liquid chromatography, the method used in this thesis, which is an established workhorse and a versatile tool in many different disciplines. It can be used for identification and quantification of interesting compounds generally present in low concentrations, called analytical scale chromatography. It can also be used for isolation and purification of high value compounds, called preparative chromatography. The latter is usually conducted in large scale with high concentrations. With high co

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Lampert, Berhard Michael. "Reverse phase HPLC of basic, neutral and acidic drugs on underivatized silica /." 1989. http://www.gbv.de/dms/bs/toc/128383976.pdf.

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Chen, Hai-Feng, and 陳海峰. "Application of Reverse Phase HPLC to Measure Progesterone Concentrations in Serum and Whole Milk of Goats after Breeding." Thesis, 1991. http://ndltd.ncl.edu.tw/handle/42054156908056049657.

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碩士<br>國立中興大學<br>畜牧學系<br>79<br>The purpose of this experiment was to established the reverse phase high performance liquid chromatography (HPLC) system to measure progesterone simply in serum and whole milk without the procedure of liquid-liquid fraction and preparing derivative. The key point of this rapid assay method was emphasised on the extraction method, the conditions and sensetivity of reverse phase HPLC and application this method in the early pregnancy diagnosis of goat. Progesterone in serum and whole milk was extracted by RP-18 cartridge or by both Si aand HP-18 cartridges. Pro

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Hung, Tsui-Hwa Tracy. "Determination of L-ascorbyl 6-palmitate in breadmaking using reverse-phase high-performance liquid chromatography (HPLC) with electrochemical (EC) detection." 1986. http://hdl.handle.net/2097/22083.

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"Reversed-Phase HPLC Determination of Cholesterol in Food Items." East Tennessee State University, 2007. http://etd-submit.etsu.edu/etd/theses/available/etd-0111107-170955/.

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"Reversed-Phase HPLC Determination of Citral in Locally Grown Lemon Grass Harvested at Different Season." East Tennessee State University, 2006. http://etd-submit.etsu.edu/etd/theses/available/etd-0705106-190437/.

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"Reversed â Phase HPLC Determination of Alliin in Diverse Varieties of Fresh Garlic and Commercial Garlic Products." East Tennessee State University, 2009. http://etd-submit.etsu.edu/etd/theses/available/etd-0707109-204459/.

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Hauenstein, Evelyn [Verfasser]. "Feinkartierung eines Intervalls auf Chromosom 11p bei sporadischen Ovarialkarzinomen : ein Vergleich von Ion-Pair Reversed-Phase HPLC und Kapillarelektrophorese / Evelyn Hauenstein." 2005. http://d-nb.info/979067006/34.

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Schaeffer, Luther Sterling. "Determination of norepinephrine in rat tissue by reversed-phase HPLC separation and amperometric detection using a boron doped nanocrystalline thin film electrode." Diss., 2006.

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(5929574), Yuan Chen. "PHOTOLYTIC LABELING TO PROBE PEPTIDE-MATRIX INTERACTIONS IN LYOPHILIZED SOLIDS." Thesis, 2020.

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<p>Therapeutic proteins are often lyophilized with excipients such as sucrose or trehalose to protect them during manufacturing and achieve a longer shelf-life. Formulation design for therapeutic proteins has been a trial-and-error process, and the mechanisms responsible for the stabilizing effects of excipients are not fully understood. Two proposed theories have been widely accepted: the water replacement theory and the vitrification theory.<sup>1,2</sup>The water replacement theory suggests that excipients stabilize protein molecules in the solid state by forming hydrogen bonds that “replac

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