Academic literature on the topic 'Serum starvation'

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Journal articles on the topic "Serum starvation"

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Wang, Yi, Juan Gao, Bojun Fan, et al. "Effects of Long-Term Serum Starvation on Autophagy, Metabolism, and Differentiation of Porcine Skeletal Muscle Satellite Cells." Veterinary Sciences 12, no. 1 (2024): 11. https://doi.org/10.3390/vetsci12010011.

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This study investigated the effects of long-term serum starvation on autophagy, metabolism, and differentiation of porcine skeletal muscle satellite cells (SMSCs) and elucidated the role of autophagy in skeletal muscle development. Our findings provide a theoretical basis for improving meat production in domestic pigs. The SMSCs isolated and preserved in our laboratory were revived and divided into six groups based on the culture medium serum concentration to simulate varying levels of serum starvation: 20% serum (control group), 15% serum (mild serum starvation group), 5% serum (severe serum
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Pirkmajer, Sergej, and Alexander V. Chibalin. "Serum starvation: caveat emptor." American Journal of Physiology-Cell Physiology 301, no. 2 (2011): C272—C279. http://dx.doi.org/10.1152/ajpcell.00091.2011.

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Serum starvation is one of the most frequently performed procedures in molecular biology and there are literally thousands of research papers reporting its use. In fact, this method has become so ingrained in certain areas of research that reports often simply state that cells were serum starved without providing any factual details as to how the procedure was carried out. Even so, we quite obviously lack unequivocal terminology, standard protocols, and perhaps most surprisingly, a common conceptual basis when performing serum starvation. Such inconsistencies not only hinder interstudy compara
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Ching, James Kain, Pooja Rajguru, Nandhini Marupudi, Sankha Banerjee, and Jonathan S. Fisher. "A role for AMPK in increased insulin action after serum starvation." American Journal of Physiology-Cell Physiology 299, no. 5 (2010): C1171—C1179. http://dx.doi.org/10.1152/ajpcell.00514.2009.

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Serum starvation is a common cell culture procedure for increasing cellular response to insulin, though the mechanism for the serum starvation effect is not understood. We hypothesized that factors known to potentiate insulin action [e.g., AMP-activated protein kinase (AMPK) and p38] or to be involved in insulin signaling leading to glucose transport [e.g., Akt, PKCζ, AS160, and ataxia telangiectasia mutated (ATM)] would be phosphorylated during serum starvation and would be responsible for increased insulin action after serum starvation. L6 myotubes were incubated in serum-containing or serum
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Dong, Su, Andrew Khoo, Jianxin Wei, et al. "Serum starvation regulates E-cadherin upregulation via activation of c-Src in non-small-cell lung cancer A549 cells." American Journal of Physiology-Cell Physiology 307, no. 9 (2014): C893—C899. http://dx.doi.org/10.1152/ajpcell.00132.2014.

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E-cadherin is essential for the integrity of adherens junctions between lung epithelial cells, and the loss of E-cadherin allows cell motility and is thought to promote lung cancer metastasis. While the downregulation of E-cadherin expression has been well characterized and is seen with transforming growth factor-β1 (TGF-β1) exposure, few studies have focused on E-cadherin upregulation. Here, we show that serum starvation causes increased E-cadherin expression via the activation of c-Src kinase in non-small-cell lung cancer A549 cells. Serum starvation increased E-cadherin protein levels in a
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Concepcion, Marc B., and David R. Nelson. "Expression of spoT in Borrelia burgdorferi during Serum Starvation." Journal of Bacteriology 185, no. 2 (2003): 444–52. http://dx.doi.org/10.1128/jb.185.2.444-452.2003.

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ABSTRACT Borrelia burgdorferi, the causative agent of Lyme disease, is transmitted by the tick Ixodes scapularis. A 2.9-kb fragment containing a putative spoT gene was isolated from B. burgdorferi genomic DNA by PCR amplification and cloned into a pBAD24 vector. The cloned gene complemented Escherichia coli mutant strain CF1693, which contains deletions of both the relA and spoT genes. The spoT gene in E. coli encodes a bifunctional enzyme capable of synthesizing and degrading (p)ppGpp, which mediates the stringent response during carbon source starvation. B. burgdorferi has been reported to h
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Sadeghian-Nodoushan, Fatemeh, Poopak Eftekhari-Yazdi, Azam Dalman, Hossein Eimani, and Houri Sepehri. "Mimosine As Well As Serum Starvation Can Be Used for Cell Cycle Synchronization of Sheep Granulosa Cells." Chinese Journal of Biology 2014 (January 2, 2014): 1–7. http://dx.doi.org/10.1155/2014/851736.

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This study was evaluated the effect of different synchronization protocols such as serum starvation for 1–3 days, confluency and chemical inhibitors on synchronization accuracy at G0/G1, apoptosis, and DNA synthesis in sheep granulosa cells. The cells were obtained from ovarian antral follicles of slaughtered sheep and used at first and fifth passages. Flow cytometry analysis showed that confluent cells, serum starvation for 24, 48, and 72 hours, and mimosine treatment significantly increased G0/G1 phase cells when compared to normally growing cells (P<0.05). Nocodazole treatment increased
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Jungers, Courtney F., Jonah M. Elliff, Daniela S. Masson-Meyers, Christopher J. Phiel, and Sofia Origanti. "Regulation of eukaryotic translation initiation factor 6 dynamics through multisite phosphorylation by GSK3." Journal of Biological Chemistry 295, no. 36 (2020): 12796–813. http://dx.doi.org/10.1074/jbc.ra120.013324.

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Eukaryotic translation initiation factor 6 (eIF6) is essential for the synthesis of 60S ribosomal subunits and for regulating the association of 60S and 40S subunits. A mechanistic understanding of how eIF6 modulates translation in response to stress, specifically starvation-induced stress, is lacking. We here show a novel mode of eIF6 regulation by glycogen synthase kinase 3 (GSK3) that is predominantly active in response to serum starvation. Both GSK3α and GSK3β phosphorylate human eIF6. Multiple residues in the C terminus of eIF6 are phosphorylated by GSK3 in a sequential manner. In respons
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Wittenstein, Amnon, Michal Caspi, Yifat David, Yamit Shorer, Prathamesh T. Nadar-Ponniah, and Rina Rosin-Arbesfeld. "Serum starvation enhances nonsense mutation readthrough." Journal of Molecular Medicine 97, no. 12 (2019): 1695–710. http://dx.doi.org/10.1007/s00109-019-01847-0.

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An, So-Young, Hyun-Kyoung Yoon, Kyoung-Sook Kim, et al. "Upregulation of human GD3 synthase (hST8Sia I) gene expression during serum starvation-induced osteoblastic differentiation of MG-63 cells." PLOS ONE 18, no. 11 (2023): e0293321. http://dx.doi.org/10.1371/journal.pone.0293321.

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In this study, we have firstly elucidated that serum starvation augmented the levels of human GD3 synthase (hST8Sia I) gene and ganglioside GD3 expression as well as bone morphogenic protein-2 and osteocalcin expression during MG-63 cell differentiation using RT-PCR, qPCR, Western blot and immunofluorescence microscopy. To evaluate upregulation of hST8Sia I gene during MG-63 cell differentiation by serum starvation, promoter area of the hST8Sia I gene was functionally analyzed. Promoter analysis using luciferase reporter assay system harboring various constructs of the hST8Sia I gene proved th
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Kim, Hak-Ryul, Ju-Ah Jeong, Chan-Hee Park, Suk-Kyeong Lee, Won-Keun Lee, and Yong-Suk Jang. "A role for cell cycle proteins in the serum-starvation resistance of Epstein–Barr virus immortalized B lymphocytes." Biochemistry and Cell Biology 80, no. 4 (2002): 407–13. http://dx.doi.org/10.1139/o02-085.

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Epstein–Barr virus (EBV) is a B-lymphotropic human herpes virus that infects B lymphocytes and is associated with a broad spectrum of benign and malignant diseases. B cell infection by EBV causes indefinite cell proliferation that results in the development of immortalized lymphoblastoid cell lines (LCLs). We found that SNU-1103, a latency type III EBV-transformed LCL developed from a Korean cancer patient, resisted the G1 arrest that was normally caused by serum starvation. Western blot analyses revealed several alterations in the expression of key regulatory cell cycle proteins involved in t
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Dissertations / Theses on the topic "Serum starvation"

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Pham, Chinh Nghia. "Le métabolisme de l'inflammation dans les maladies microcristallines." Electronic Thesis or Diss., Université Paris Cité, 2024. http://www.theses.fr/2024UNIP5246.

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La goutte, due aux dépôts de cristaux d'urate monosodique (UMS), et la maladie liée aux dépôts des cristaux de pyrophosphate de calcium (PPC) sont les deux rhumatismes inflammatoires les plus fréquents de l'adulte. Les cristaux sont responsables de crises inflammatoires aiguës et récurrentes qui dépendent de la production de l'interleukine (IL)-1 beta via l'activation de l'inflammasome NLRP3. Ces crises inflammatoires peuvent être modulées par des facteurs environnementaux tels que les régimes alimentaires dont la restriction calorique (RC) ou le jeûne intermittent (JI). Les objectifs de ma th
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Chang, Han-Ming, and 張漢銘. "Induction of apoptosis by insulin in glioma after serum starvation." Thesis, 1995. http://ndltd.ncl.edu.tw/handle/72656589837956574928.

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碩士<br>國立成功大學<br>微生物及免役學研究所<br>83<br>Glioblastoma is the most common and malignant brain tumors in the human central nervous system (CNS). As yet, the mechanism of tumor progression is not well understood. In the CNS, insulin promotes the growth and development of neuron and acts as a neuronmodulator. The objective of this study was to examine the influence on the early growth phase of gliomas after serum starvation. In our culture system, glioma U-373MG cells grew as reported more faster in
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Wen-FengLiu and 劉玟妦. "Serum Starvation Induces VEGF-C Expression in Human Intrahepatic Cholangiocarcinoma Cells." Thesis, 2018. http://ndltd.ncl.edu.tw/handle/wpk2jn.

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"Identification of native protein of a novel peroxisome proliferator-activated receptor alpha (PPAR[alpha]) target gene-PPAR[alpha]-regulated and starvation inducible gene (PPSIG) by production of polyclonal antisera." 2007. http://library.cuhk.edu.hk/record=b5893094.

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Yau Wing Yiu, Winifred.<br>On t.p. "alpha"s appear as the Greek letter.<br>Thesis submitted in: October 2006.<br>Thesis (M.Phil.)--Chinese University of Hong Kong, 2007.<br>Includes bibliographical references (leaves 91-98).<br>Abstracts in English and Chinese.<br>Abstract --- p.i<br>Abstract (Chinese version) --- p.iv<br>Acknowledgements --- p.vi<br>Table of Contents --- p.vii<br>List of Abbreviations --- p.xii<br>List of Figures --- p.xiv<br>List of Tables --- p.xvi<br>Chapter Chapter 1 --- Introduction --- p.1<br>Chapter 1.1 --- Peroxisome proliferator-activated receptors (PPARs) --
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Books on the topic "Serum starvation"

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Neligan, Patrick J., and Clifford S. Deutschman. Pathophysiology and causes of metabolic acidosis in the critically ill. Oxford University Press, 2016. http://dx.doi.org/10.1093/med/9780199600830.003.0255.

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Critical illness is typically characterized by changes in the balance of water and electrolytes in the extracellular space, resulting in the accumulation of anionic compounds that manifests as metabolic acidosis. Metabolic acidosis manifests with tachypnoea, tachycardia, vasodilatation, headache and a variety of other non-specific symptoms and signs. It is caused by a reduction in the strong ion difference (SID) or an increase in weak acid concentration (albumin or phosphate). Increased SID results from hyperchloraemia, haemodilution or accumulation of metabolic by-products. A reduction in SID
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Neligan, Patrick J., and Clifford S. Deutschman. Management of metabolic acidosis in the critically ill. Oxford University Press, 2016. http://dx.doi.org/10.1093/med/9780199600830.003.0256.

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Metabolic acidosis (MA) commonly complicates critical illness, usually manifesting as a fall in arterial pH (&lt;7.4) accompanied by a concomitant fall in serum bicarbonate concentration. Acidosis caused by unmeasured anions (UMA), can be distinguished from Hyperchloraemic acidosis by demonstrating a widening of the anion gap (AG). AG should be corrected for albumin and lactate. The base deficit (BD) calculates degree of metabolic acidosis and represents the amount of strong cation required to restore the pH to 7.4. Neither the AG nor the BD specify the cause of acidosis, and are unhelpful in
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Book chapters on the topic "Serum starvation"

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Woo, Yeon I., Hyun Joo Son, Hye Ryeon Lim, et al. "Effects Of (1→3), (1→6)-β-D-Glucan Behavior in Human Dermal Fibroblast Cells under Serum Starvation." In Advanced Biomaterials VII. Trans Tech Publications Ltd., 2007. http://dx.doi.org/10.4028/0-87849-436-7.401.

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Peeters, Robin P., and Anita Boelen. "Non-Thyroidal Illness (NTI)." In Oxford Textbook of Endocrinology and Diabetes 3e, edited by John A. H. Wass, Wiebke Arlt, and Robert K. Semple. Oxford University Press, 2021. http://dx.doi.org/10.1093/med/9780198870197.003.0042.

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Already a few hours after the onset of acute illness, marked changes in serum thyroid hormone levels occur. This is referred to as non-thyroidal illness (NTI) also known as the low T<sub>3</sub> syndrome and the euthyroid sick syndrome. The most characteristic and persistent abnormality is a low serum T<sub>3</sub>. Nevertheless, patients usually have no clinical signs of thyroid dysfunction. A low T<sub>3</sub> in euthyroid patients is also seen during caloric deprivation. Both in NTI and in fasting there is a negative energy balance in the majority of cases. Therefore, the low levels of T<su
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Peeters, R. P. "Nonthyroidal illness." In Oxford Textbook of Endocrinology and Diabetes. Oxford University Press, 2011. http://dx.doi.org/10.1093/med/9780199235292.003.3044.

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A few hours after the onset of acute illness, marked changes in serum thyroid hormone levels occur. This is referred to as nonthyroidal illness (NTI). The most characteristic and persistent abnormality is a low level of serum triiodothyronine (T<sub>3</sub>). Despite these low levels of serum T<sub>3</sub>, patients usually have no clinical signs of thyroid disease. Other terms for this disease state have been used, e.g. the low T<sub>3</sub> syndrome and the euthyroid sick syndrome. In addition to nonthyroidal illness, a low T<sub>3</sub> in euthyroid patients is seen during caloric deprivati
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Vu, Binh Thanh, Hanh Thi Le, Khanh Nha Nguyen, and Phuc Van Pham. "Hypoxia, Serum Starvation, and TNF-α Can Modify the Immunomodulation Potency of Human Adipose-Derived Stem Cells." In Advances in Experimental Medicine and Biology. Springer International Publishing, 2021. http://dx.doi.org/10.1007/5584_2021_672.

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Conference papers on the topic "Serum starvation"

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Venkatadri, Rajkumar, Juan Sebastian Yakisich, Neelam Azad, and Anand Krishnan V. Iyer. "Abstract 254: Lung cancer cells growing under prolonged period of serum starvation display increased stemness." In Proceedings: AACR 107th Annual Meeting 2016; April 16-20, 2016; New Orleans, LA. American Association for Cancer Research, 2016. http://dx.doi.org/10.1158/1538-7445.am2016-254.

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Lee, Sun-Hye, Youn-Sang Jung, Ji-Yun Chung, Ah-Young Oh, Su-Jin Lee, and Bum-Joon Park. "Abstract B3: Induction of Smad4 in response to serum starvation promotes cell death through PUMA stabilization." In Abstracts: Second AACR International Conference on Frontiers in Basic Cancer Research--Sep 14-18, 2011; San Francisco, CA. American Association for Cancer Research, 2011. http://dx.doi.org/10.1158/1538-7445.fbcr11-b3.

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