Relevant bibliographies by topics / Shigella flexneri biochemical reactions

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Academic literature on the topic 'Shigella flexneri biochemical reactions'

Author: Grafiati
Published: 3 June 2025
Last updated: 31 July 2025

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Journal articles on the topic "Shigella flexneri biochemical reactions"

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Padilla, Carlos, Verónica Carrasco-Sánchez, Andrés Padilla, and Olga Lobos. "Partial Characterization of Novel Bacteriocin SF1 Produced by Shigella flexneri and Their Lethal Activity on Members of Gut Microbiota." International Journal of Microbiology 2019 (May 6, 2019): 1–6. http://dx.doi.org/10.1155/2019/6747190.

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A strain of Shigella flexneri producing bacteriocin was isolated from a patient with diarrhea. The main objective of this study was to isolate and partially characterize the bacteriocin. The producing microorganism was identified using biochemical, serological, and molecular methods. The lethal activity of the S. flexneri strain was studied using the drop method. This bacterial strain showed activity against different strains of E. coli and B. fragilis. Using immunological techniques, it was determined that S. flexneri belongs to serotype 2a, and by PCR, the presence of the ipaH plasmid was de
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Sarker, Shafiuzzaman, Reeashat Muhit Neeloy, Marnusa Binte Habib, et al. "Mobile Colistin-Resistant Genes mcr-1, mcr-2, and mcr-3 Identified in Diarrheal Pathogens among Infants, Children, and Adults in Bangladesh: Implications for the Future." Antibiotics 13, no. 6 (2024): 534. http://dx.doi.org/10.3390/antibiotics13060534.

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Colistin is a last-resort antimicrobial for treating multidrug-resistant Gram-negative bacteria. Phenotypic colistin resistance is highly associated with plasmid-mediated mobile colistin resistance (mcr) genes. mcr-bearing Enterobacteriaceae have been detected in many countries, with the emergence of colistin-resistant pathogens a global concern. This study assessed the distribution of mcr-1, mcr-2, mcr-3, mcr-4, and mcr-5 genes with phenotypic colistin resistance in isolates from diarrheal infants and children in Bangladesh. Bacteria were identified using the API-20E biochemical panel and 16s
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Schroeder, Gunnar N., and Hubert Hilbi. "Molecular Pathogenesis of Shigella spp.: Controlling Host Cell Signaling, Invasion, and Death by Type III Secretion." Clinical Microbiology Reviews 21, no. 1 (2008): 134–56. http://dx.doi.org/10.1128/cmr.00032-07.

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SUMMARY Shigella spp. are gram-negative pathogenic bacteria that evolved from harmless enterobacterial relatives and may cause devastating diarrhea upon ingestion. Research performed over the last 25 years revealed that a type III secretion system (T3SS) encoded on a large plasmid is a key virulence factor of Shigella flexneri. The T3SS determines the interactions of S. flexneri with intestinal cells by consecutively translocating two sets of effector proteins into the target cells. Thus, S. flexneri controls invasion into EC, intra- and intercellular spread, macrophage cell death, as well as
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Aliprantis, Antonios O., David S. Weiss, Justin D. Radolf, and Arturo Zychlinsky. "Release of Toll-Like Receptor-2-Activating Bacterial Lipoproteins in Shigella flexneri Culture Supernatants." Infection and Immunity 69, no. 10 (2001): 6248–55. http://dx.doi.org/10.1128/iai.69.10.6248-6255.2001.

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ABSTRACT Shigella spp. cause dysentery, a severe form of bloody diarrhea. Apoptosis, or programmed cell death, is induced during Shigella infections and has been proposed to be a key event in the pathogenesis of dysentery. Here, we describe a novel cytotoxic activity in the sterile-culture supernatants of Shigella flexneri. An identical activity was identified in purified S. flexneri endotoxin, defined here as a mixture of lipopolysaccharide (LPS) and endotoxin-associated proteins (EP). Separation of endotoxin into EP and LPS revealed the activity to partition exclusively to the EP fraction. B
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Zakir Hossain, A. K. M., Md Zahid Hasan, Sohana Akter Mina, Nahid Sultana, and A. M. Masudul Azad Chowdhury. "Occurrence of shigellosis in pediatric diarrheal patients in Chattogram, Bangladesh: A molecular based approach." PLOS ONE 18, no. 6 (2023): e0275353. http://dx.doi.org/10.1371/journal.pone.0275353.

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Shigellaa Gram-negative, non-motile bacillus, is the primary causative agent of the infectious disease shigellosis, which kills 1.1 million people worldwideevery year. The children under the age of five are primarily the victims of this disease. This study has been conducted to assess the prevalence of shigellosis through selective plating, biochemical test and conventional PCR assays, where the samples were collected from suspected diarrheoal patients. Invasive plasmid antigen H (ipaH) and O-antigenic rfc gene were used to identify Shigella spp. and S. flexneri respectively. For validation of
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Ledov, Vladimir A., Marina E. Golovina, Biana I. Alkhazova, Vyacheslav L. Lvov, Alexander L. Kovalchuk, and Petr G. Aparin. "A Pentavalent Shigella flexneri LPS-Based Vaccine Candidate Is Safe and Immunogenic in Animal Models." Vaccines 11, no. 2 (2023): 345. http://dx.doi.org/10.3390/vaccines11020345.

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A multivalent vaccine is much needed to achieve protection against predominant Shigella serotypes. Recently, we demonstrated the clinical applicability and immunogenic potential of tri-acylated S. flexneri 2a lipopolysaccharide (Ac3-S-LPS). Using a similar approach, we designed a pentavalent LPS candidate vaccine against S. flexneri 1b, 2a, 3a, 6, and Y (PLVF). In this study, we performed molecular and antigenic characterization of the vaccine candidate and its preclinical evaluation. There were no signs of acute toxicity after subcutaneous administration of PLVF in rabbits at a proposed human
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Gentle, Amy, Philip M. Ashton, Timothy J. Dallman, and Claire Jenkins. "Evaluation of Molecular Methods for Serotyping Shigella flexneri." Journal of Clinical Microbiology 54, no. 6 (2016): 1456–61. http://dx.doi.org/10.1128/jcm.03386-15.

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Shigella flexnerican be phenotypically serotyped using antisera raised to type-specific somatic antigens and group factor antigens and genotypically serotyped using PCR targeting O-antigen synthesis or modification genes. The aim of this study was to evaluate a real-time PCR for serotypingS. flexneriand to use whole-genome sequencing (WGS) to investigate the phenotypic and genotypic serotype identifications. Of the 244 cultures tested retrospectively, 226 (92.6%) had concordant results between phenotypic serotyping and PCR. Seventy of the 244 isolates (including 15 of the 18 isolates where a s
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Sen, Tanuka, and Naresh K. Verma. "YfiB: An Outer Membrane Protein Involved in the Virulence of Shigella flexneri." Microorganisms 10, no. 3 (2022): 653. http://dx.doi.org/10.3390/microorganisms10030653.

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The intracellular pathogen Shigella flexneri, which is the causative agent of bacillary dysentery, significantly influences the worldwide implication of diarrheal infections, consequentially causing about 1.1 million deaths each year. Due to a nonavailability of an authorized vaccine and the upsurge of multidrug resistance amongst Shigella strains, there has been a huge demand for further genetic analyses which could help in the advancement of new/improved drugs, and finding vaccine candidates against the pathogen. The present study aims to illustrate the role of the yfiB gene in Shigella viru
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Fries, Louis F., Andrew D. Montemarano, Corey P. Mallett, David N. Taylor, Thomas L. Hale, and George H. Lowell. "Safety and Immunogenicity of a Proteosome-Shigella flexneri 2a Lipopolysaccharide Vaccine Administered Intranasally to Healthy Adults." Infection and Immunity 69, no. 7 (2001): 4545–53. http://dx.doi.org/10.1128/iai.69.7.4545-4553.2001.

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ABSTRACT We studied the safety and immunogenicity of a Shigella flexneri 2a vaccine comprising native S. flexneri 2a lipopolysaccharide (LPS) complexed to meningococcal outer membrane proteins—proteosomes—in normal, healthy adults. A two-dose series of immunizations was given by intranasal spray, and doses of 0.1, 0.4, 1.0, and 1.5 mg (based on protein) were studied in a dose-escalating design. The vaccine was generally well tolerated. The most common reactions included rhinorrhea and nasal stuffiness, which were clearly dose related (P ≤ 0.05). These reactions were self-limited and generally
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Belhu, Tegegn, Kinfe Fissehatsion, Abraham Tesfaye, Dr Yohannes Woldekidan, and Kassu Desta. "Prevalence of Intestinal Parasites and Gastrointestinal Carriage of Pathogenic Gram Negative Enteric Bacteria among Apparently Healthy Food Handlers of Public Hospitals, Addis Ababa, Ethiopia." International Journal of Microbiology 2020 (November 17, 2020): 1–9. http://dx.doi.org/10.1155/2020/8867033.

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Background. Foodborne diseases are major public health problems in developing countries like Ethiopia. Food handlers with poor personal hygiene working in hospitals could be infected with different intestinal parasites and pathogenic enteric bacteria. Therefore, they could pose a potential risk of foodborne infection to patients and the community. Methods. An institutional based cross-sectional study conducted from March to June 2017. Besides, sociodemographic data were collected using a structured questionnaire, freshly passed stool specimens for direct wet mount smear examination, and formal
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Conference papers on the topic "Shigella flexneri biochemical reactions"

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Floares, Doris, Diana Obistioiu, Anca Hulea, Ersilia Alexa, and Isidora Radulov. "THUJA OCCIDENTALIS AND PLATYCLADUS ORIENTALIS ANTIMICROBIAL ACTIVITY." In 23rd SGEM International Multidisciplinary Scientific GeoConference 2023. STEF92 Technology, 2023. http://dx.doi.org/10.5593/sgem2023v/6.2/s25.57.

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The rise of bacterial resistance to currently employed antibiotics is causing growing concerns for public health. The emergence of highly resistant bacterial strains results in the ineffectiveness of antibiotic treatments against many bacterial infections. As a result, there is an ongoing quest for new antimicrobial agents. This pursuit can take two main directions: one involves the design and synthesis of novel agents, while the other involves exploring natural sources to uncover previously undiscovered antimicrobial compounds. Herbal medications, particularly, have garnered renewed interest
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