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Relevant bibliographies by topics / Spot-on lawn assay

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Journal articles

Academic literature on the topic 'Spot-on lawn assay'

Author: Grafiati

Published: 3 June 2025

Last updated: 31 July 2025

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Journal articles on the topic "Spot-on lawn assay"

1

Esra, Demir, and Başbülbül Gamze. "Screening of Bacteriocin Production in Lactic Acid Bacteria Isolated From Fermented Dairy Products." Biotechnology Journal International 18, no. 2 (2017): 1–9. https://doi.org/10.9734/BJI/2017/33504.

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<strong>Aims: </strong>In this study we aimed to screen LAB strains from different fermented dairy products by phenotypic and genotypic methods. <strong>Methodology:</strong> 138 LAB isolates belonging to <em>Enterococcus</em> (13,76%), <em>Lactococcus</em> (18,84%) and <em>Lactobacillus</em> (67,39%) genera from fermented dairy products were used to search for their bacteriocin production and bacteriocin encoding genes were searched. LAB isolates were screened by spot-on lawn assay method against indicator microorganisms. <em>Geobacillus stearothermophilus</em> DSMZ 22, <em>Escherichia coli</

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Zwarycz, Allison S., and David E. Whitworth. "Myxobacterial Predation: A Standardised Lawn Predation Assay Highlights Strains with Unusually Efficient Predatory Activity." Microorganisms 11, no. 2 (2023): 398. http://dx.doi.org/10.3390/microorganisms11020398.

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Myxobacteria prey upon a broad range of microorganisms. Lawn assays are commonly used to quantify myxobacterial predation—myxobacterial suspensions are spotted onto prey lawns, and monitored via spot expansion. The diversity in motility behaviours of myxobacterial strains and differing assay protocols in myxobacteriology laboratories led us to develop a highly-specified assay, which was applied to 28 myxobacterial strains preying on seven phytopathogenic prey species. Generally, prey organisms showed no qualitative differences in their susceptibility/resistance to myxobacterial predation. For

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3

Poimenidou, Sofia V., Athina Skarveli, Georgia Saxami, Evdokia K. Mitsou, Maria Kotsou, and Adamantini Kyriacou. "Inhibition of Listeria monocytogenes Growth, Adherence and Invasion in Caco-2 Cells by Potential Probiotic Lactic Acid Bacteria Isolated from Fecal Samples of Healthy Neonates." Microorganisms 11, no. 2 (2023): 363. http://dx.doi.org/10.3390/microorganisms11020363.

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Lactic acid bacteria (LAB) isolated from healthy humans may prove an effective tool against pathogen growth, adherence and invasion in intestinal epithelial cells. This study aimed to evaluate the antilisterial properties of LAB isolated from fecal samples of healthy neonates. Forty-five LAB strains were tested for their antimicrobial activity against ten Listeria monocytogenes strains with spot-on-lawn and agar-well diffusion assays, and ten lactobacilli strains were further assessed for their inhibitory effect against adherence and invasion of Caco-2 cells by L. monocytogenes EGDe. Inhibitio

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4

Aruta, Kathleen Joy C., and Christopher Marlowe A. Caipang. "Local aquatic microflora as a potential source of probionts in biofloc technology for whiteleg shrimp, <i>Penaeus vannamei</i>." Environmental and Experimental Biology 23, no. 1 (2025): 1–8. https://doi.org/10.22364/eeb.23.01.

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The Philippine shrimp aquaculture industry, a key supplier of Penaeus vannamei to domestic and international markets, faces significant disease challenges, particularly from pathogenic Vibrio species. Current disease management strategies often employ terrestrially-sourced probiotics, whose efficacy can be limited by environmental incompatibility with the aquatic host. This study aimed to address this limitation by isolating and characterizing putative probiotic microorganisms from the local aquatic environment of P. vannamei. Water samples, collected from a shrimp farm outlet pond in Negros O

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5

Mussi, Jamili Maria Suhet, Guilherme Campos Tavares, Ethiene Luiza de Souza Santos, et al. "Antagonistic activity of lactic acid bacteria isolated from Minas artisanal cheeses against Brucella abortus." Brazilian Journal of Veterinary Research and Animal Science 60 (August 21, 2023): e204539. http://dx.doi.org/10.11606/issn.1678-4456.bjvras.2023.204539.

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This study aimed to evaluate methods for studying the in vitro antimicrobial activity of lactic acid bacteria (LAB) against Brucella abortus and to evaluate the antagonistic effect of LAB on the viability of this pathogen. A total of 18 LAB strains (Lactobacillus plantarum, n = 11; Pediococcus acidilactici, n = 1; Lactobacillus rhamnosus, n = 4; and Lactobacillus brevis, n = 2), isolated from Minas artisanal cheeses produced in three regions (Canastra, Campos das Vertentes, and Araxá) of Minas Gerais State, Brazil, were tested for their antimicrobial activity against B. abortus using three met

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Ali, Zainab. "Screening of marcescin producing isolates from Serratia marcescens." Academic Science Journal 2, no. 4 (2024): 207–16. http://dx.doi.org/10.24237/asj.02.04.811d.

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The current study intends to investigate the production of bacteriocin from S. marcescens, a Gram-negative bacterium, is a human pathogen and the reason why hospital medical equipment becomes contaminated. Various samples from patients about 220 samples which include blood,urine,abscess and sputum, twenty -three isolates of S.marcescens was performed as per standard laboratory procedure and confirmed by using VITEK-2 systems. The isolates of S.marcescens which produced marcescin detected by four methods : spot –on-the –lawn method , Cup-assay method ,Well-diffusion method and Agar cross streak

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7

Seccareccia, Ivana, Christian Kost, and Markus Nett. "Quantitative Analysis of Lysobacter Predation." Applied and Environmental Microbiology 81, no. 20 (2015): 7098–105. http://dx.doi.org/10.1128/aem.01781-15.

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ABSTRACTBacteria of the genusLysobacterare considered to be facultative predators that use a feeding strategy similar to that of myxobacteria. Experimental data supporting this assumption, however, are scarce. Therefore, the predatory activities of threeLysobacterspecies were tested in the prey spot plate assay and in the lawn predation assay, which are commonly used to analyze myxobacterial predation. Surprisingly, only one of the testedLysobacterspecies showed predatory behavior in the two assays. This result suggested that not allLysobacterstrains are predatory or, alternatively, that the a

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8

Venegas-Ortega, María Georgina, Adriana Carolina Flores-Gallegos, Cristóbal Noé Aguilar, Raúl Rodríguez-Herrera, José Luis Martínez-Hernández, and Guadalupe Virginia Nevárez-Moorillón. "Multi-Functional Potential of Presumptive Lactic Acid Bacteria Isolated from Chihuahua Cheese." Foods 9, no. 3 (2020): 276. http://dx.doi.org/10.3390/foods9030276.

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The multifunctional properties of autochthonous lactic acid bacteria can be of use for enhancing the sensorial properties of food, as well as in food preservation. An initial screening for antimicrobial, proteolytic, and lipolytic capacities was done in 214 presumptive lactic acid bacteria isolates obtained from Chihuahua cheese manufacturing and during a ripening period of nine months. The antimicrobial screening was done by spot-on-the-lawn tests, using Listeria monocytogenes and Escherichia coli as indicator microorganisms; proteolysis was tested in casein-peptone agar and lipolysis in Mann

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9

Wan, Allison, Cherry Ibarra-Romero, John Vederas, and Sorina Chiorean. "Learning the Science Behind Bacteriocins Through Lacticin 3147; a Promising Lantibiotic." Alberta Academic Review 2, no. 2 (2019): 79–80. http://dx.doi.org/10.29173/aar75.

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As microorganisms continue to develop resistance and survive against many different forms of antimicrobial solutions, such as antibiotics, the threat that antimicrobial resistance poses grows considerably. One solution to this persistent issue could be bacteriocins: ribosomally synthesized antimicrobial peptides that are synthesized by bacteria. In this study, the specific lantibiotic—a subclass of bacteriocin—used was lacticin 3147, which is comprised of two components: A1 and A2. Lacticin 3147 was first purified and isolated in order to properly analyze its antimicrobial effects, which show

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10

Rozoy, Elodie, Laurent Bazinet, Monica Araya-Farias, Anthony Guernec, and Linda Saucier. "Inhibitory Effects of Commercial and Enriched Green Tea Extracts on the Growth of Brochothrix thermosphacta, Pseudomonas putida and Escherichia coli." Journal of Food Research 2, no. 1 (2012): 1. http://dx.doi.org/10.5539/jfr.v2n1p1.

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<p>The major catechin found in green tea, called epigallocatechin gallate (EGCG), have been reported to have antimicrobial properties. In this study, we examined <em>in vitro </em>the antimicrobial effects of a commercial green tea extract sold in a capsule form, and two prepared green tea extracts enriched in catechins against <em>Brochothrix thermosphacta</em>, <em>Pseudomonsas putida</em> and <em>Escherichia coli</em> which have been associated with meat spoilage. The antimicrobial activity of the different tea extracts was evaluated by

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