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Journal articles on the topic 'Total viable bacteria'

Author: Grafiati

Published: 3 June 2025

Last updated: 31 July 2025

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1

NAKAMURA, TOSHIKO, and TARO YOSHIKURA. "Total bacteria and viable bacteria in Lake Biwa and Yodo River." Bulletin of Japanese Society of Microbial Ecology 5, no. 1 (1990): 13–20. http://dx.doi.org/10.1264/microbes1986.5.13.

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Muñoz-Elías, Ernesto J., Juliano Timm, Tania Botha, Wai-Tsing Chan, James E. Gomez, and John D. McKinney. "Replication Dynamics of Mycobacterium tuberculosis in Chronically Infected Mice." Infection and Immunity 73, no. 1 (2005): 546–51. http://dx.doi.org/10.1128/iai.73.1.546-551.2005.

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ABSTRACT The dynamics of host-pathogen interactions have important implications for the design of new antimicrobial agents to treat chronic infections such as tuberculosis (TB), which is notoriously refractory to conventional drug therapy. In the mouse model of TB, an acute phase of exponential bacterial growth in the lungs is followed by a chronic phase characterized by relatively stable numbers of bacteria. This equilibrium could be static, with little ongoing replication, or dynamic, with continuous bacterial multiplication balanced by bacterial killing. A static model predicts a close corr

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3

Ding, Yun, and Yong Guang Yin. "A close to Real-Time Intelligent System for Enumerating Total Viable Bacteria Based on Pattern Recognition Technology." Applied Mechanics and Materials 741 (March 2015): 344–49. http://dx.doi.org/10.4028/www.scientific.net/amm.741.344.

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In this paper, a novel close to real-time artificial intelligent system for enumerating Total Viable Bacteria (TVB) in drinking water was developed by using pattern recognition and machine vision technology. In order to identify the viable bacteria accurately, four shape features including circularity ratio, eccentricity, rectangularity, and compact degree, and four color features (GRsd, BRsd, HRsd, SRsd) of the stained viable bacteria image were extracted. An optimal artificial neural network was used as the bacterial recognition classifier, whose inputs were the extracted feature parameters

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Sawaya, K., N. Kaneko, K. Fukushi, and J. Yaguchi. "Behaviors of physiologically active bacteria in water environment and chlorine disinfection." Water Science and Technology 58, no. 7 (2008): 1343–48. http://dx.doi.org/10.2166/wst.2008.445.

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Direct microscopic methods using several fluorescent staining were applied to estimate the proportion of physiologically active bacteria in the water environment and evaluate the efficacy of disinfection with chlorine. 4′,6-diamidino-2-phenylindole (DAPI) was used to determine total bacterial numbers, and 5-cyano-2,3-ditolyl tetrazolium chloride (CTC) was chosen for direct detection of respiring bacteria. BacLightTM kit was used to assess bacterial membrane integrity. Bacteria with growth potential were enumerated using the DVC method and microcolony technique. The total bacterial number in ri

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Stinson, Lisa F., Michelle L. Trevenen, and Donna T. Geddes. "The Viable Microbiome of Human Milk Differs from the Metataxonomic Profile." Nutrients 13, no. 12 (2021): 4445. http://dx.doi.org/10.3390/nu13124445.

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Bacteria in human milk contribute to the establishment of the infant gut microbiome. As such, numerous studies have characterized the human milk microbiome using DNA sequencing technologies, particularly 16S rRNA gene sequencing. However, such methods are not able to differentiate between DNA from viable and non-viable bacteria. The extent to which bacterial DNA detected in human milk represents living, biologically active cells is therefore unclear. Here, we characterized both the viable bacterial content and the total bacterial DNA content (derived from viable and non-viable cells) of fresh

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Leon, Ingrid M., Brent W. Auvermann, K. Jack Bush, et al. "Comprehensive Analysis of E. coli, Enterococcus spp., Salmonella enterica, and Antimicrobial Resistance Determinants in Fugitive Bioaerosols from Cattle Feedyards." Applied Microbiology 5, no. 3 (2025): 63. https://doi.org/10.3390/applmicrobiol5030063.

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Antimicrobial use in food animals selects for antimicrobial-resistant (AMR) bacteria, which most commonly reach humans via the food chain. However, AMR bacteria can also escape the feedyard via agricultural runoff, manure used as crop fertilizer, and even dust. A study published in 2015 reported AMR genes in dust from cattle feedyards; however, one of the study’s major limitations was the failure to investigate gene presence in viable bacteria, or more importantly, viable bacteria of importance to human health. Our main objective was to investigate the presence and quantity of viable bacteria

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Chen, Lihua, Xuan Zhu, Menglu Zhang, et al. "Profiling Total Viable Bacteria in a Hemodialysis Water Treatment System." Journal of Microbiology and Biotechnology 27, no. 5 (2017): 995–1004. http://dx.doi.org/10.4014/jmb.1612.12002.

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8

Hyvärinen, A. M., P. J. Martikainen, and A. I. Nevalainen. "Suitability of poor medium in counting total viable airborne bacteria." Grana 30, no. 2 (1991): 414–17. http://dx.doi.org/10.1080/00173139109432000.

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9

Baena-Ruano, S., C. Jiménez-Ot, I. M. Santos-Dueñas, D. Cantero-Moreno, F. Barja, and I. García-García. "Rapid method for total, viable and non-viable acetic acid bacteria determination during acetification process." Process Biochemistry 41, no. 5 (2006): 1160–64. http://dx.doi.org/10.1016/j.procbio.2005.12.016.

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Kyoui, Daisuke, Yuka Saito, Akifumi Takahashi, et al. "Antibacterial Activity of Hexanol Vapor In Vitro and on the Surface of Vegetables." Foods 12, no. 16 (2023): 3097. http://dx.doi.org/10.3390/foods12163097.

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Hexanol is a volatile alcohol and a major component of plant essential oils (EOs). However, the antibacterial activity of hexanol vapor has not been well studied. This study aimed to evaluate the antibacterial activity of hexanol. In this study, seven food-related bacteria were exposed to 1-, 2- or 3-hexanol vapor on agar media to evaluate their growth. Additionally, the total viable counts in three vegetables when exposed to 1-hexanol vapor were measured. The results showed that 1-hexanol exhibited antibacterial effects against Gram-negative bacteria but did not affect Gram-positive bacteria.

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11

Wang, Xiaoyang, Ruohan Liang, Xiaodan Pu, et al. "Application of the Electrical Microbial Growth Analyzer Method for Efficiently Quantifying Viable Bacteria in Ready-to-Eat Sea Cucumber Products." Microorganisms 12, no. 11 (2024): 2301. http://dx.doi.org/10.3390/microorganisms12112301.

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Accurate and efficient quantification of viable bacteria in ready-to-eat food products is crucial for food safety and public health. The rapid and accurate assessment of foodborne bacteria in complex food matrices remains a significant challenge. Herein a culture-based approach was established for easily quantifying viable bacteria in ready-to-eat sea cucumber (RSC) products. Samples of the liquid companion within the package were directly transferred into test tubes to determine bacterial growth curves and growth rate curves, utilizing the electrical microbial growth analyzer. Viable bacteria

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Tong, Yingjia, Zeqing Wang, Qunyi Tong, and Yutong Liu. "Effects of Lactic Acid Bacteria Fermentation and In Vitro Simulated Digestion on the Bioactivities of Purple Sweet Potato Juice." Foods 13, no. 24 (2024): 4094. https://doi.org/10.3390/foods13244094.

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The effects of lactic acid bacteria fermentation and in vitro simulated digestion on phenolic bioavailability, phenolic bioavailability, and antioxidant activity of purple sweet potato juice (PSPJ) were investigated. The PSPJ was fermented by Lactobacillus rhamnosus and Streptococcus thermophilus. The viable bacterial count, phenolic components, antioxidant activity, phenolic bioaccessibility, and phenolic bioavailability of PSPJ were analyzed during the simulated digestion process in vitro. The data displayed that lactic acid bacteria fermentation increased total α-glucosidase inhibition, tot

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Oladele, Paul, and Timothy A. Johnson. "PSV-12 Evaluating the effect of lyoprotectants in preserving community structure and bacterial viability in the lyophilized fecal community of pigs." Journal of Animal Science 102, Supplement_3 (2024): 511. http://dx.doi.org/10.1093/jas/skae234.578.

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Abstract Fecal microbiota transplantation (FMT) stands as a potential method for enhancing pig health. FMT has progressed from the basic delivery of fresh stool, to lyophilized preparations which is beneficial for sample storage, but can damage bacterial cells. This has necessitated the need for lyoprotectants to preserve bacterial viability. However, the ideal lyoprotectant for retaining community structure and viability remains unclear. Furthermore, DNA-based microbial profiling methods such as 16S rRNA and metagenomics sequencing do not distinguish live and dead bacteria, prompting the need

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Sjollema, Jelmer, Minie Rustema-Abbing, Henny C. van der Mei, and Henk J. Busscher. "Generalized Relationship between Numbers of Bacteria and Their Viability in Biofilms." Applied and Environmental Microbiology 77, no. 14 (2011): 5027–29. http://dx.doi.org/10.1128/aem.00178-11.

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ABSTRACTBacterial biofilms are confined communities that are encapsulated in protective layers of extracellular polymeric substances. Microscopic evaluation of biofilms of diverse bacterial strains on various substrata reveals that, in general, the percentage of viable bacteria decreases with the total number of bacteria in a biofilm.

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Asiya, P., Manjula Menon, Girigan Gopi, and Vipin Das. "Impact of Cropping Systems on Soil Microbial Load: Evidence from Wetland Ecosystems of Wayanad District, Kerala." Mapana - Journal of Sciences 18, no. 4 (2019): 1–8. http://dx.doi.org/10.12723/mjs.51.1.

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This study enumerated the microbial communities in soil samples from rice, banana and arecanut from the wet lands of Wayanad district Kerala. The total viable bacterial count in the paddy field was 120x10-6 cfu, 121x10-6 cfu and 147x10-6 cfu in Nenmeni, Kaniyambatta and Pozhuthana Gramapanchayat and that of fungi was 30x10-3 cfu, 32 x10-3 cfu and 37 x10-3 cfu. Likewise, the total viable count of bacteria in areca nut at Nenmeni, Kaniyambatta and Pozhuthana Gramapanchayat was 66 x10-6 cfu, 80 x10-6 cfu, 118 x10-6 cfu and that of fungi was 14 x10-3 cfu, 18 x10-3 cfu, and 30 x10-3 cfu. The total

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Newbold, C. J., F. M. McIntosh, and R. J. Wallace. "Changes in the microbial population of a rumen-simulating fermenter in response to yeast culture." Canadian Journal of Animal Science 78, no. 2 (1998): 241–44. http://dx.doi.org/10.4141/a97-086.

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Yeast increased the total viable count of bacteria on a medium containing rumen fluid and soluble sugars by 38% (P < 0.05) and the cellulolytic population increased by 49%, although the increase was not statistically significant (P > 0.05). Microscopic counts indicated a 25% increase in total bacteria (P < 0.05). Bacterial counts with mannitol as sole energy source also increased (P < 0.05), indicating an increased Selenomonas population; that was not in itself sufficient to account for the increase in the total bacterial count. The protozoal population was significantly lower in y

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Kowalski, Michał, Jozef S. Pastuszka, Agnieszka Brąszewska, Josef Cyrys, and Ewa Brągoszewska. "Airborne Bacteria in Gliwice—The Industrialized City in Poland." Atmosphere 13, no. 10 (2022): 1721. http://dx.doi.org/10.3390/atmos13101721.

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The results of the study on the characteristics of the viable (culturable) and total bacterial particles in the ambient air in Gliwice, Poland, are presented. The concentration of viable bacteria in the air ranged from 57 CFU m−3 (Colony Forming Units per cubic meter) during winter to 305 CFU m−3 in spring, while the concentration of all bacteria (live and dead) in the air, measured in selected days, ranged from 298 cells m−3 in winter to over 25 thousand per m3 in autumn. A field study was also carried out to find out the level of the sterilization rate (k) for airborne bacteria. The obtained

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18

Sansupa, Chakriya, Sara Fareed Mohamed Wahdan, Terd Disayathanoowat, and Witoon Purahong. "Identifying Hidden Viable Bacterial Taxa in Tropical Forest Soils Using Amplicon Sequencing of Enrichment Cultures." Biology 10, no. 7 (2021): 569. http://dx.doi.org/10.3390/biology10070569.

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This study aims to estimate the proportion and diversity of soil bacteria derived from eDNA-based and culture-based methods. Specifically, we used Illumina Miseq to sequence and characterize the bacterial communities from (i) DNA extracted directly from forest soil and (ii) DNA extracted from a mixture of bacterial colonies obtained by enrichment cultures on agar plates of the same forest soil samples. The amplicon sequencing of enrichment cultures allowed us to rapidly screen a culturable community in an environmental sample. In comparison with an eDNA community (based on a 97% sequence simil

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Mahmoud, M. Abo-El-Saad, E. I. Badawy Mohamed, and M. M. Mohammed Youssef. "ANALYSIS AND IDENTIFICATION OF MICROBIAL SPECIES ASSOCIATED WITH THE EGYPTIAN DATE FRUITS DURING POSTHARVEST STORAGE." Journal of Applied Biological Sciences 17, no. 1 (2023): 138–54. https://doi.org/10.5281/zenodo.7579923.

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Date fruits are consumed in many countries globally and are mostly loaded with a mixture of microbes. As a result, various bacterial and fungal species attack them, thus causing their spoilage at the ripening, storage, and processing stages. The present study investigates the current spoilage bacteria and fungi of twelve postharvest date fruits collected from different Egyptian geographical regions (Aswan, Siwa, and El-Wady El-Gadeed). Isolation and identification of date-borne microbes based on morphological and molecular identification were studied. The microscopic examination of infected da

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Haque, Md Mansurul, MG Sorrowar, and Harun Ur Rashid. "Effects of frozen storage, radiation and their combined Treatments on microorganisms of freshwater mola fish Amblypharyngodon mola." Journal of Bangladesh Academy of Sciences 37, no. 1 (2013): 21–31. http://dx.doi.org/10.3329/jbas.v37i1.15677.

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The study revealed that the total viable bacterial count, staphylococcal count, coliform count, faecal coliform count, Aeromonas count and total fungal counts varied from 8.8×107 to 1.27×108, 2.7×106 to 4.2×106, 9.0×104 to 1.03×105, 4.8×105 to 7.3×105, 2.7×105 to 5.0×105 and 1.6×103 to 3.5×103 cfu/gm, respectively. Seventy six bacterial strains were isolated and identified, where Staphylococcus aureus, Micrococcus varians, Aeromonas hydrophila, Klebsiella ozaenae, Bacillus subtilis, Escherichia coli, Bacillus megaterium, Klebsiella edwardsii, Pseudomonas aerugenosa and Micrococcus radiodurans

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21

Umeanaeto, P. U., E. G. Ani, V. I. Anyaoha, J. U. Anumba, S. C. Afulukwe, and C. C. Okoli. "Parasitic and bacterial contamination of sachets water sold at Ngwo, Enugu State, Nigeria." Nigerian Journal of Parasitology 43, no. 2 (2022): 303–10. http://dx.doi.org/10.4314/njpar.v43i2.13.

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Water is an essential part of human nutrition. It is readily available in sachets with little or no interest in the quality by the producers. This study focused on the isolation and identification of parasites and bacteria contaminating sachet water sold at Ngwo, Enugu State, Nigeria. Twenty-one sachets of water were collected randomly from seven different brands sold within the community. The parasites were identified using sedimentation technique while bacteria were isolated from water cultured in Nutrient, MacConkey, and Eosin Methylene Blue Agar media. The physicochemical parameters analys

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Ahmed, Egbal Osman, and Mohamed Eltaher Ali. "Effect of the Seasons and Salt Concentrations on Microbial Load of Wet-Salted Fermented Product (Fassiekh)." Al-Mukhtar Journal of Sciences 32, no. 1 (2017): 46–53. http://dx.doi.org/10.54172/mjsc.v32i1.93.

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The study aims at investigating the microbial load of wet-salted fermented product (a traditional fermented product in Sudan named locally; fassiekh) and determination of total viable bacterial count, according to salt concentrations (20%, 25% and 30% of the fish weight) and seasons (summer, autumn and winter) variations. Fessiekh processed from popular fessiekh fish species (Hydrocynus spp, local name: Kass).The isolation and identification of bacteria and mould were examined for the microbial quality of fassiekh. The total viable count of bacteria in fresh fish used as raw materials in fessi

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Helmi, K., F. Barthod, G. Méheut, et al. "Methods for microbiological quality assessment in drinking water: a comparative study." Journal of Water and Health 13, no. 1 (2014): 34–41. http://dx.doi.org/10.2166/wh.2014.056.

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The present study aimed to compare several methods for quantifying and discriminating between the different physiological states of a bacterial population present in drinking water. Flow cytometry (FCM), solid-phase cytometry (SPC), epifluorescence microscopy (MSP) and culture method performances were assessed by comparing the results obtained for different water samples. These samples, including chlorinated and non-chlorinated water, were collected in a drinking water treatment plant. Total bacteria were quantified by using SYBR Green II (for FCM) and 4′,6′-diamino-2-phenylindole (DAPI) (for

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HUTCHISON, M. L., L. D. WALTERS, G. C. MEAD, M. HOWELL, and V. M. ALLEN. "An Assessment of Sampling Methods and Microbiological Hygiene Indicators for Process Verification in Poultry Slaughterhouses." Journal of Food Protection 69, no. 1 (2006): 145–53. http://dx.doi.org/10.4315/0362-028x-69.1.145.

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Studies to determine the appropriateness of the use of populations of indicator bacteria on poultry carcasses for process verification were undertaken in commercial slaughterhouses. Samples were collected from neck skin by excision or from whole carcass rinses and were examined for a range of presumptive process hygiene indicator bacteria. Coefficients of variation were calculated for each bacterial indicator and were significantly lower in excised samples, indicating more reproducible bacterial recovery by this sampling method. Total viable counts of aerobic bacteria, Enterobacteriaceae, and

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Stinson, Lisa F., Michelle L. Trevenen, and Donna T. Geddes. "Effect of Cold Storage on the Viable and Total Bacterial Populations in Human Milk." Nutrients 14, no. 9 (2022): 1875. http://dx.doi.org/10.3390/nu14091875.

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Expression and cold storage of human milk is a common practice. Current guidelines for cold storage of expressed milk do not take into account the impact on the milk microbiome. Here, we investigated the impact of cold storage on viable bacterial populations in human milk. Freshly expressed milk samples (n = 10) were collected and analysed immediately, stored at 4 °C for four days, −20 °C for 2.25 months and 6 months, and −80 °C for 6 months. Samples were analysed using propidium monoazide (PMA; a cell viability dye) coupled with full-length 16S rRNA gene. An aliquot of each sample was additio

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FERNANDEZ-PIQUER, JUDITH, JOHN P. BOWMAN, TOM ROSS, SILVIA ESTRADA-FLORES, and MARK L. TAMPLIN. "Preliminary Stochastic Model for Managing Vibrio parahaemolyticus and Total Viable Bacterial Counts in a Pacific Oyster (Crassostrea gigas) Supply Chain." Journal of Food Protection 76, no. 7 (2013): 1168–78. http://dx.doi.org/10.4315/0362-028x.jfp-12-401.

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Vibrio parahaemolyticus can accumulate and grow in oysters stored without refrigeration, representing a potential food safety risk. High temperatures during oyster storage can lead to an increase in total viable bacteria counts, decreasing product shelf life. Therefore, a predictive tool that allows the estimation of both V. parahaemolyticus populations and total viable bacteria counts in parallel is needed. A stochastic model was developed to quantitatively assess the populations of V. parahaemolyticus and total viable bacteria in Pacific oysters for six different supply chain scenarios. The

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Šuster, Katja, and Andrej Cör. "Induction of Viable But Non-Culturable State in Clinically Relevant Staphylococci and Their Detection with Bacteriophage K." Antibiotics 12, no. 2 (2023): 311. http://dx.doi.org/10.3390/antibiotics12020311.

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Prosthetic joint infections are frequently associated with biofilm formation and the presence of viable but non-culturable (VBNC) bacteria. Conventional sample culturing remains the gold standard for microbiological diagnosis. However, VBNC bacteria lack the ability to grow on routine culture medium, leading to culture-negative results. Bacteriophages are viruses that specifically recognize and infect bacteria. In this study, we wanted to determine if bacteriophages could be used to detect VBNC bacteria. Four staphylococcal strains were cultured for biofilm formation and transferred to low-nut

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Lee, Sulhee, and Young-Seo Park. "Analysis of Microflora Profile in the Manufacturing Process of Commercial Tofu." Food Engineering Progress 16, no. 3 (2012): 270–77. http://dx.doi.org/10.13050/foodengprog.2012.16.3.270.

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Microflora profiles including total bacteria, psychrophiles, and spore-forming bacteria in manufacturing process steps of tofu were analyzed to elucidate the cause of microbial contamination. The viable cell numbers of total bacteria in soybean and packed tofu were 4.75 and 2.53 log CFU/g, respectively. The viable cell number of psychrophiles in soybean was calculated to 3.55 log CFU/g, and reduced to 0.78 log CFU/g by heating during the soymilk preparation step, and was not detected in the final product. The viable cell numbers of spore-forming bacteria, the major bacteria in soymilk, in soyb

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Cyprowski, Marcin, Anna Ławniczek-Wałczyk, Agata Stobnicka-Kupiec, Małgorzata Gołofit-Szymczak, and Rafał L. Górny. "Across-Shift Changes in Viable Nasal Bacteria among Waste-Incineration Plant Workers—A Pilot Study." International Journal of Environmental Research and Public Health 19, no. 15 (2022): 8984. http://dx.doi.org/10.3390/ijerph19158984.

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The aim of this pilot study was to assess the time-related changes in viable nasal bacteria concentrations among waste-incineration plant (WIP) workers compared to a group of office building (OB) workers outside the plant. In total, 20 volunteers participated in the study, including 14 WIP and 6 OB workers. WIP workers were divided into two sub-groups: supervisory staff (SVS) and maintenance and repair workers (MRW). Nasal swabs were collected before and after the morning work shift. Airborne bacteria were sampled with a six-stage impactor to assess the bioaerosol size distribution. The analys

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Newbold, C. J., R. J. Wallace, and F. M. Mcintosh. "Mode of action of the yeastSaccharomyces cerevisiaeas a feed additive for ruminants." British Journal of Nutrition 76, no. 2 (1996): 249–61. http://dx.doi.org/10.1079/bjn19960029.

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Two suggested modes of action of yeast in stimulating rumen fermentation were investigated. The first, that yeast respiratory activity protects anaerobic rumen bacteria from damage by O2, was tested using different strains of yeast that had previously been shown to have differing abilities to increase the viable count of rumen bacteria.Saccharomyces cerevisiaeNCYC 240, NCYC 1026, and the commercial product Yea-Sacc®, added to rumen fluidin vitroat 1·3 mg/ml, increased the rate of O2disappearance by between 46 and 89%. The same three preparations also stimulated bacterial numbers in anin vitrof

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Chidia, Ugwuowo Leonard, Umeakuana Doris Chidinma, Ofokansi Chidimma Gloria, Okoh Favour Chinenye, and Adum Chioma Precious. "EFFECT OF DIETS CONTAINING BIOCHAR AND CLAY ON THE SERUM BIOCHEMISTRY, HEMATOLOGICAL INDICES AND TOTAL BACTERIA VIABLE COUNT IN BROILER." Reviews In Food And Agriculture 5, no. 2 (2024): 76–81. https://doi.org/10.26480/rfna.02.2024.76.81.

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The study was conducted to determine the effect of diets containing biochar and clay on the hematological indices, serum biochemistry and total bacterial viable count of broiler. Ninety six broilers were allocated to four treatments diets having three replicates of eight broilers in a completely randomized design. Data were collected on red blood cell count (RBC), white blood cell count (WBC), hemoglobin concentration (Hb), hematocrit (HCT), platelets count, lymphocytes, mean corpuscular hemoglobin (MCH), mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration (MCHC) levels, c

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Ahmed Mohamed Moawad Mabrouk, Ahmed Mohamed Moawad Mabrouk. "Assessment of the Microbial Occupational Work Environment in One Faculty of King Abdulaziz University in Jeddah." journal of King Abdulaziz University - Meteorology, Environment and Arid Land Agriculture Sciences 26, no. 2 (2015): 75–82. http://dx.doi.org/10.4197/met.26-2.8.

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The presence of microorganisms in indoor air can pose serious health problems so; the purpose of the present work was to monitor the different groups of microorganisms including total viable bacteria, Staphylococci, total coliforms, Salmonella and fungi in different sites of the occupational environment (laboratories, offices, corridors and toilets) inside the Faculty of Metrology, Environment and Arid Land Agriculture, King Abdulaziz University, Jeddah, Saudi Arabia. The results showed that, the average of total viable bacterial counts in different work laboratories was ranged from 9500 to 23

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Dupray, E., M. Pommepuy, A. Derrien, M. P. Caprais, and M. Cormier. "Use of the Direct Viable Count (D.V.C.) For the Assessment of Survival of E. Coli in Marine Environments." Water Science and Technology 27, no. 3-4 (1993): 395–99. http://dx.doi.org/10.2166/wst.1993.0380.

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The survival of the enterotoxigenic Escherichia coli H 10407 was observed in different marine seawaters, both in situ and in vitro by use of dialysis chambers and microcosms, exposed and not exposed to light. Survival was monitored during several days by measurement of plate counts (culturable bacteria), direct viable counts with yeast extract and nalidixic acid (DVC : viable bacteria), and acridine orange direct counts (total bacteria). Without light, culturable E. coli counts decreased slowly (1.5 log unit), whereas viable and total bacteria counts remained almost the same through 8 days. Wh

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Aksoy, Alev, Nizami Duran, Serdar Toroglu, and Fatih Koksal. "Short-term Effect of Mastic Gum on Salivary Concentrations of Cariogenic Bacteria in Orthodontic Patients." Angle Orthodontist 77, no. 1 (2007): 124–28. http://dx.doi.org/10.2319/122205-455r.1.

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Abstract Objective: To determine antibacterial activity of chewing mastic gum against the salivary levels of Streptococcus mutans, the total number of viable bacteria, and lactobacilli in patients undergoing therapy with fixed orthodontic appliances. Materials and Methods: In this study, the levels of S mutans, lactobacilli, and total cultivated bacteria were measured before and after chewing mastic gum. The antibacterial effects of chewing mastic gum against these microorganisms in saliva were compared with a placebo gum. The counts for orthodontically treated patients were evaluated before c

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Horve, Patrick F., Leslie G. Dietz, Suzanne L. Ishaq, Jeff Kline, Mark Fretz, and Kevin G. Van Den Wymelenberg. "Viable bacterial communities on hospital window components in patient rooms." PeerJ 8 (July 27, 2020): e9580. http://dx.doi.org/10.7717/peerj.9580.

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Previous studies demonstrate an exchange of bacteria between hospital room surfaces and patients, and a reduction in survival of microorganisms in dust inside buildings from sunlight exposure. While the transmission of microorganisms between humans and their local environment is a continuous exchange which generally does not raise cause for alarm, in a hospital setting with immunocompromised patients, these building-source microbial reservoirs may pose a risk. Window glass is often neglected during hospital disinfection protocols, and the microbial communities found there have not previously b

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Nur, I. T., M. Talukder, T. R. Das, M. Asaduzzaman, F. Feroz, and S. K. Munshi. "Microbiological status of some street iftar items collected from chalk bazar in Dhaka city, Bangladesh." Food Research 5, no. 3 (2021): 236–40. http://dx.doi.org/10.26656/fr.2017.5(3).617.

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In Dhaka, the capital of Bangladesh and one of the most densely populated cities of the world, different categories of street foods are widely consumed by all classes of people, especially for iftar during the holy month of Ramadan. The objective of this research was to assess the microbiological quality of street iftar food items collected from a street in Chalk Bazar locality of Dhaka along with the antibiogram profile of the bacterial isolates. A total of 74 samples belong to ten different categories of street food items and 8 different types of street vended juices were collected aseptical

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Buchanan-Mappin, J. M., P. M. Wallis, and A. G. Buchanan. "Enumeration and identification of heterotrophic bacteria in groundwater and in a mountain stream." Canadian Journal of Microbiology 32, no. 2 (1986): 93–98. http://dx.doi.org/10.1139/m86-020.

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Populations of heterotrophic bacteria were enumerated from stream and groundwater samples taken from an undisturbed catchment basin in southwestern Alberta. Direct counts using epifluorescence microscopy were compared with total viable counts using standard plate count methods, the iodonitrotetrazolium formazan method (reduction of 2-(p-iodophenyl)-3-(p-nitro phenyl)-5-phenyl tetrazolium chloride to iodonitrotetrazolium formazan), the nalidixic acid method, and the slide culture method. The nalidixic acid method gave the highest results, with total viable counts as high as 34.6% of the total d

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Odo, Joel Inya, Ebah EE, and Eberechukwu AM. "Bacterial Contamination of Drinking Wells in Makurdi Metropolis." International Journal of Oceanography & Aquaculture 8, no. 2 (2024): 1–9. http://dx.doi.org/10.23880/ijoac-16000307.

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This study was carried out to assess the bacterial contamination of drinking wells in Makurdi metropolis. Fifteen (15) untreated water samples, five (5) each were collected from wells in High Level, Wurukum and Wadata areas for physicochemical and bacteriological analysis was done using pour plating and the bacteriological quality was assessed by measuring the total viable, total coliform and fecal coliform counts of bacteria in the samples using Mac Conkey agar and Nutrient agar. The results of the analysis showed that the pH ranged from 6.70 ± 0.16 to 7.40± 0.87, the temperature levels range

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JI, Odo. "Bacterial Contamination of Drinking Wells in Makurdi Metropolis." Open Access Journal of Microbiology & Biotechnology 9, no. 2 (2024): 1–9. http://dx.doi.org/10.23880/oajmb-16000295.

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This study was carried out to assess the bacterial contamination of drinking wells in Makurdi metropolis. Fifteen (15) untreated water samples, five (5) each were collected from wells in High Level, Wurukum and Wadata areas for physicochemical and bacteriological analysis was done using pour plating and the bacteriological quality was assessed by measuring the total viable, total coliform and fecal coliform counts of bacteria in the samples using Mac Conkey agar and Nutrient agar. The results of the analysis showed that the pH ranged from 6.70 ± 0.16 to 7.40± 0.87, the temperature levels range

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Nsereko, V. L., K. A. Beauchemin, D. P. Morgavi, et al. "Effect of a fibrolytic enzyme preparation from Trichoderma longibrachiatum on the rumen microbial population of dairy cows." Canadian Journal of Microbiology 48, no. 1 (2002): 14–20. http://dx.doi.org/10.1139/w01-131.

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The effects of supplementing a dairy cow diet with incremental levels of a fibrolytic enzyme preparation (preparation B) from Trichoderma longibrachiatum on the rumen microbial population were investigated. Two cows fitted with rumen cannulae were each fed a diet containing barley-based concentrate (52%), maize silage (29%), and chopped alfalfa hay (19%), supplemented with 0, 1, 2, 5, or 10 L of preparation B per tonne of dry matter (DM). Preparation B stimulated numbers of total viable bacteria in a quadratic manner (P < 0.05), to approximately 230, 330, 390, and 250% at 1, 2, 5, and 10 L·

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Alam, Nur E., Md Rayhan Ali, Md Tarek Molla, Shahin Mahmud, Kaisar Ali Talukder, and AKM Mohiuddin. "Therapeutic Potential of Plant Extracts Against Multidrug Resistance Poultry Bacteria." Plant Tissue Culture and Biotechnology 30, no. 1 (2020): 119–30. http://dx.doi.org/10.3329/ptcb.v30i1.47797.

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Plant extracts were evaluated on bacteria isolated from poultry farm for developing substitutive therapeutic agent of antibiotics. A diverse range of bacterial load observed both in total viable count (TVC) and in total coliform count (TCC) in 30 samples randomly collected from poultry feeds, drinking water and faeces. A total of six bacterial isolates e.g. Pseudomonas spp., Aeromonas spp., Citrobacter spp., Vibrio spp., Escherichia coli and Plesiomonas spp. were found in the samples cultured in MacConkey Agar medium. Fifteen antibiotics were studied against bacterial susceptibility. All the b

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Muhammad, Ekhlas Uddin, Akter Tanzina, Anowar Khasru Parvez Md., et al. "Microbial Safety of Street Vended Fruit Juices in Dhaka City of Bangladesh." Journal of Advances in Microbiology 3, no. 2 (2017): 1–7. https://doi.org/10.9734/JAMB/2017/33651.

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Freshly squeezed fruit juice is a nutritious drink. Its appealing look and attractive taste make it a popular drink among many different aged people. But the risk due to fresh juice consumption is very important. <strong>Aims: </strong>The present study was undertaken to investigate the microbiological quality of freshly squeezed juices and to determine their safety for human consumption. <strong>Place and Duration of Study:</strong> This study was conducted in Microbiology Department of Primeasia University, Banani, Dhaka-1213, Bangladesh during February, 2015 to April, 2015. <strong>Methodol

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Timoracká, Ivana, Simona Kunová, Viktória Zachar Lovászová, et al. "MICROBIOLOGICAL QUALITY ASSESSMENT OF RAINBOW TROUT (ONCORHYNCHUS MYKISS) MEAT TREATED WITH ROSEMARY AND THYME DRIED HERBS AND ESSENTIAL OILS." Journal of microbiology, biotechnology and food sciences 14, no. 2 (2024): e11157. http://dx.doi.org/10.55251/jmbfs.11157.

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This study investigates the effects of thyme and rosemary dried herbs, as well as thyme and rosemary essential oils (1%, w/v), on the microbiological quality of vacuum-packed rainbow trout meat stored under refrigerated conditions (4 ± 1 °C) for 7 days. The microbiological quality of meat was monitored based on total viable counts, coliform bacteria, and lactic acid bacteria counts over a designated storage period (on the 0th, 1st, 3rd, 5th and 7th day of storage). As expected, the highest total viable counts were observed on the 7th day of storage in the untreated aerobically packed control g

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Luna, G. M., E. Manini, and R. Danovaro. "Large Fraction of Dead and Inactive Bacteria in Coastal Marine Sediments: Comparison of Protocols for Determination and Ecological Significance." Applied and Environmental Microbiology 68, no. 7 (2002): 3509–13. http://dx.doi.org/10.1128/aem.68.7.3509-3513.2002.

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ABSTRACT It is now universally recognized that only a portion of aquatic bacteria is actively growing, but quantitative information on the fraction of living versus dormant or dead bacteria in marine sediments is completely lacking. We compared different protocols for the determination of the dead, dormant, and active bacterial fractions in two different marine sediments and at different depths into the sediment core. Bacterial counts ranged between (1.5 ± 0.2) × 108 cells g−1 and (53.1 ± 16.0) × 108 cells g−1 in sandy and muddy sediments, respectively. Bacteria displaying intact membrane (liv

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Haidinger, W., M. P. Szostak, W. Jechlinger, and W. Lubitz. "Online Monitoring of Escherichia coli Ghost Production." Applied and Environmental Microbiology 69, no. 1 (2003): 468–74. http://dx.doi.org/10.1128/aem.69.1.468-474.2003.

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ABSTRACT Controlled expression of cloned φX174 gene E in gram-negative bacteria results in lysis of the bacteria by the formation of a transmembrane tunnel structure built through the cell envelope complex. Production of bacterial ghosts is routinely monitored by classical microbiological procedures. These include determination of the turbidity of the culture and the total number of cells and the number of reproductive cells present during the time course of growth and lysis. Although conceptually simple, these methods are labor intensive and time consuming, providing a complete set of results

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Абдуллаева, Асият Мухтаровна, Лариса Петровна Блинкова, Борис Вениаминович Уша, Румия Камилевна Валитова, Юрий Дмитриевич Пахомов, and Дарья Борисовна Митрофанова. "Detection of Viable but Nonculturable Microbial Cells in Chicken Mince." Health, Food & Biotechnology 1, no. 4 (2019): 26–38. http://dx.doi.org/10.36107/hfb.2019.i4.s281.

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The relevance of the study and the presence of gaps in the relevant knowledge on the topic. Potential existence of hazardous viable but nonculturable (VBNC) cells of pathogenic microorganisms in foodstuffs that can be formed under the influence of various factors, their detection and determination of conditions for formation of VBNC cells of various contaminant bacteria are relevant for preventing contamination of meats.Methods. In the study, a search was conducted for VBNC cells in chicken mince in real time and during experimental infection of it by Staphylococcus aureus 209P. In order to de

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La Duc, Myron T., Anne Dekas, Shariff Osman, Christine Moissl, David Newcombe, and Kasthuri Venkateswaran. "Isolation and Characterization of Bacteria Capable of Tolerating the Extreme Conditions of Clean Room Environments." Applied and Environmental Microbiology 73, no. 8 (2007): 2600–2611. http://dx.doi.org/10.1128/aem.03007-06.

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ABSTRACT In assessing the bacterial populations present in spacecraft assembly, spacecraft test, and launch preparation facilities, extremophilic bacteria (requiring severe conditions for growth) and extremotolerant bacteria (tolerant to extreme conditions) were isolated. Several cultivation approaches were employed to select for and identify bacteria that not only survive the nutrient-limiting conditions of clean room environments but can also withstand even more inhospitable environmental stresses. Due to their proximity to spacefaring objects, these bacteria pose a considerable risk for for

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Shu, Guowei, Mengqi Tian, Binyun Cao, Changfeng Wang, and Ni Xin. "Effects of Stachyose on Synbiotic Yogurt Obtained from Goat Milk with Lactobacillus acidophilus and Lactobacillus casei." Acta Universitatis Cibiniensis. Series E: Food Technology 22, no. 2 (2018): 43–50. http://dx.doi.org/10.2478/aucft-2018-0011.

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Abstract Development of synbiotic yogurt having multiple health benefits has become a new trend. The purpose of this study was to obtain the optimum stachyose concentration of the goat yogurt fermented by probiotics including Lactobacillus acidophilus (LA) or Lactobacillus casei (LC) besides Streptococcus thermophilus and Lactobacillus bulgaricus by measuring pH, acidity, viable counts and the change of stachyose content. The optimal stachyose concentration of goat LA-yogurt and goat LC-yogurt were 0.4% and 0.8%. Under the above concentrations, the total bacteria number and the viable counts o

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Banin, E., T. Israely, A. Kushmaro, Y. Loya, E. Orr, and E. Rosenberg. "Penetration of the Coral-Bleaching BacteriumVibrio shiloi into Oculina patagonica." Applied and Environmental Microbiology 66, no. 7 (2000): 3031–36. http://dx.doi.org/10.1128/aem.66.7.3031-3036.2000.

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ABSTRACT Inoculation of the coral-bleaching bacterium Vibrio shiloi into seawater containing its host Oculina patagonica led to adhesion of the bacteria to the coral surface via a β-d-galactose receptor, followed by penetration of the bacteria into the coral tissue. The internalized V. shiloi cells were observed inside the exodermal layer of the coral by electron microscopy and fluorescence microscopy using specific anti-V. shiloi antibodies to stain the intracellular bacteria. At 29Ã¯Â¿Â½C, 80% of the bacteria bound to the coral within 8 h. Penetration, measured by the viable count (gentamici

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Blinkova, Larisa, Danik M. Martirosyan, Yury Pakhomov, Olga Dmitrieva, Rachel Vaughan, and Michael Altshuler. "Nonculturable forms of bacteria in lyophilized probiotic preparations." Functional Foods in Health and Disease 4, no. 2 (2014): 66. http://dx.doi.org/10.31989/ffhd.v4i2.29.

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Background: Nonculturable cells are formed under stress. These viable but nonculturable (VBNC) cells retain the ability to revert to active growth and division when conditions become favorable, or after treatment with resuscitating factors. Information about the possible presence of VBNC in bacterial lyophilized probiotic preparations, foodstuffs, live vaccines, etc., indicates that human as well as animal intestines are a significant area for research.Methods: Samples were stored for different periods of time (up to 30 years) according to the manufacturers’ manuals. Total counts were conducte

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