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1

Chen, Z. Y., W. M. N. Ratnayake, L. Fortier, R. Ross, and S. C. Cunnane. "Similar distribution of trans fatty acid isomers in partially hydrogenated vegetable oils and adipose tissue of Canadians." Canadian Journal of Physiology and Pharmacology 73, no. 6 (1995): 718–23. http://dx.doi.org/10.1139/y95-093.

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The objective of this study was to evaluate the composition of trans fatty acids in the subcutaneous fat of Canadians relative to the composition of dietary sources of trans fatty acids. The fatty acid composition, total trans fatty acid content, and the geometric and positional isomer distribution of unsaturated fatty acids of subcutaneous adipose tissue of Canadians were determined using a combination of capillary gas–liquid chromatography and silver nitrate thin-layer chromatography. The mean total trans fatty acid content was 6.80% at the abdominal site and 5.80% at the lateral thigh site.
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2

Vetica, Fabrizio, Anna Sansone, Carla Ferreri, and Chryssostomos Chatgilialoglu. "A convenient route to mono-trans polyunsaturated free fatty acids." Journal of Chemical Research 46, no. 2 (2022): 174751982210909. http://dx.doi.org/10.1177/17475198221090908.

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Trans unsaturated fatty acids in humans may be originated both from dietary supplementation and from an endogenous free-radical-catalyzed cis−trans isomerization of fatty acid residues in naturally occurring cis lipids. The latter process affords geometrical isomers and the polyunsaturated fatty acid mono-trans isomers were demonstrated to be connected with stress conditions in living organisms. Synthesis of mono-trans polyunsaturated fatty acid is useful for analytical and biological research, and in this case, the availability of free fatty acids is needed as well as the possibility of mg sc
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3

Paszczyk, B., Z. Żegarska, and Z. Borejszo. "the contents of trans fatty acids and cla in cow colostrum and milk fat in the early lactation period." Czech Journal of Food Sciences 23, No. 4 (2011): 159–65. http://dx.doi.org/10.17221/3386-cjfs.

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The trans fatty acids as well as cis-9 trans-11 C18:2 (CLA) were determined in the colostrum and milk fat of individual cows during up to 82 days of lactation. The analyses were performed using gas chromatography (GLC) on a capillary column (CP Sil 88) combined with the method of argentation thin-layer chromatography (Ag-TLC). The results obtained in the study indicated that the milk fat of individual cows, kept under identical living and feeding conditions, was characterised by an extremely high variability of trans isomers concentration, especially of those of C18:1 acid. The differences ref
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4

Paszczyk, Beata, Magdalena Polak-Śliwińska, and Joanna Łuczyńska. "Fatty Acids Profile, Trans Isomers, and Lipid Quality Indices in Smoked and Unsmoked Cheeses and Cheese-Like Products." International Journal of Environmental Research and Public Health 17, no. 1 (2019): 71. http://dx.doi.org/10.3390/ijerph17010071.

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The purpose of this study was to evaluate the fatty acid composition, including trans C18:1 and C18:2 isomers and the content of conjugated linoleic acid cis9trans11 C18:2 (CLA), in commercial smoked and unsmoked cheeses and cheese-like products available on the Polish market as well as to compare lipid quality indices in these products. The composition of fatty acids was determined with the gas chromatography method. The conducted study demonstrated that smoked and unsmoked cheeses as well as smoked and unsmoked cheese-like products were characterized by various contents of fatty acids and va
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5

Stepanov, Aleksey, Ol'ga Bykova, O. Kostyunina, and S. D. Pil'nikova. "Determination of the relationship between SNP genotypes and the content of fatty acids of various spatial configurations in cow milk." Agrarian Bulletin of the 24, no. 01 (2024): 108–18. http://dx.doi.org/10.32417/1997-4868-2024-24-01-108-118.

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Abstract. Milk fat is the third main source of lipids for human nutrition. Fatty acids in milk fat have a heterogeneous composition due to differences in chain length, degree of saturation, etc. It has been scientifically proven that long-term consumption of trans-fatty acids causes various diseases of the human body; according to WHO recommendations, their maximum consumption should not exceed 1 % of total energy. A comprehensive study of the composition of fatty acids in cattle milk gives scientists the opportunity to use this trait in breeding work by selecting genotypes with a low content
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6

Stepanov, Aleksey, Ol'ga Bykova, and Ol'ga Kostyunina. "FATTY ACID COMPOSITION OF BLACK-MOTTLED BREED COWS MILK OF THE URAL REGION." Bulletin of KSAU, no. 12 (January 27, 2025): 181–88. https://doi.org/10.36718/1819-4036-2022-12-181-188.

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The purpose of research is to study the content of fatty acids, trans-isomers of unsaturated fatty acids in cow's milk. Tasks: to establish the concentration of saturated and unsaturated fatty acids (%, g/100) in cows' milk, to determine the quantitative content of trans-fatty acids in the composition of milk fat (%, g/100), to identify the daily dynamics of the fatty acid composition of milk. The studies were carried out in JSC Kamenskoye of the Sverdlovsk Region on breeding cows from the first to the third lactation. The studied indicators: the amount of saturated, monounsaturated, polyunsat
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7

McKain, Nest, Kevin J. Shingfield, and R. John Wallace. "Metabolism of conjugated linoleic acids and 18 : 1 fatty acids by ruminal bacteria: products and mechanisms." Microbiology 156, no. 2 (2010): 579–88. http://dx.doi.org/10.1099/mic.0.036442-0.

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Cultures of ruminal bacteria known to metabolize unsaturated fatty acids were grown in medium containing 50 μg ml−1 of geometric and positional isomers of conjugated linoleic acid (CLA) or 18 : 1 fatty acids and 37.4 % deuterium oxide to investigate the mechanisms responsible for fatty acid metabolism. Butyrivibrio fibrisolvens JW11 converted cis-9,trans-11-18 : 2 and trans-9,trans-11-18 : 2 to trans-11-18 : 1 as the main product, labelled at C-9, and metabolized trans-10,cis-12-18 : 2 to trans-10-18 : 1, labelled at C-13, and smaller amounts of trans-12-18 : 1 and cis-12-18 : 1. Butyrivibrio
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8

Toshtay, K., and A. B. Auyezov. "NEW STEREOSELECTIVE CATALYSTS FOR THE HYDROGENATION OF SUNFLOWER OIL." Chemical Journal of Kazakhstan, no. 4 (December 15, 2023): 131–41. http://dx.doi.org/10.51580/2023-4.2710-1185.46.

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The work is devoted to the creation of new stereoselective catalysts for the hydrogenation of sunflower oil, which can significantly reduce the trans-isomers of fatty acids. Thus, on nickel catalysts used in industry, the content of trans-isomers of fatty acids reaches up to 30%. Platinum deposited on diatomite (processed natural clay) 0.2 to 1% is offered as a catalyst. Methodology. The hydrogenation was carried out in an autoclave, excluding external diffusion inhibition, at hydrogen pressures ranging from 0.25 to 1.0 MPa and temperatures ranging from 90 to 110°C. Results and discussion. Chr
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9

Watkins, Bruce A. "Influences of biotin deficiency and dietary trans-fatty acids on tissue lipids in chickens." British Journal of Nutrition 61, no. 1 (1989): 99–111. http://dx.doi.org/10.1079/bjn19890096.

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1. The combined effects of feeding hydrogenated fats and varying the levels of biotin and linoleate (18:2ω6) on polyunsaturated fatty acids were studied in the chicken.2. Biotin deficiency signs were not exacerbated by feeding hydrogenated fats or by diets low in linoleate for 21 d.3. Biotin deficiency resulted in proportionately higher levels of 18:2ω6 and γ-linolenate (18:3ω6) in liver triglycerides, and lower levels of dihomo-γ-linolenate (20:3ω6) in liver and heart phospholipids irrespective of the 18:2ω6 level in the diet.4. Biotin deficiency did not alter arachidonate (20:4ω6) levels in
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10

Auhadieva, El'vira, Rustem Daukaev, Tat'yana Larionova, Dmitriy Musabirov, Evgeniya Zelenkovskaya, and Guzel' Allayarova. "TRANSFATTY ACIDS PROFILE IN FOOD SAMPLES." Bulletin of KSAU, no. 3 (March 5, 2024): 179–85. http://dx.doi.org/10.36718/1819-4036-2024-3-179-185.

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The purpose of the study is to monitor the content of trans isomers of fatty acids in fat-containing foods. Objectives: to determine the fatty acid composition of the fat phase of food products of various categories; to calculate the content of trans isomers of fatty acids; to give a comparative description of food products based on trans fat content; to give recommendations for reducing the load of trans fats on the human body. The study was carried out in the chemical-analytical department of the Ufa Research Institute of Occupational Medicine and Human Ecology. The objects of research are f
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11

Baumgard, Lance H., Benjamin A. Corl, Debra A. Dwyer, A. Saebø, and Dale E. Bauman. "Identification of the conjugated linoleic acid isomer that inhibits milk fat synthesis." American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 278, no. 1 (2000): R179—R184. http://dx.doi.org/10.1152/ajpregu.2000.278.1.r179.

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Conjugated linoleic acids (CLA) are octadecadienoic fatty acids that have profound effects on lipid metabolism. Our previous work showed that CLA (mixture of isomers) markedly reduced milk fat synthesis. In this study, our objective was to evaluate the effects of specific CLA isomers. Multiparous Holstein cows were used in a 3 × 3 Latin square design, and treatments were 4-day abomasal infusions of 1) skim milk (control), 2) 9,11 CLA supplement, and 3) 10,12 CLA supplement. CLA supplements provided 10 g/day of the specific CLA isomer ( cis-9, trans-11 or trans-10, cis-12). Treatments had no ef
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12

Kuhnt, Katrin, Jana Kraft, Heinz Vogelsang, Klaus Eder, Jürgen Kratzsch, and Gerhard Jahreis. "Dietary supplementation with trans-11- and trans-12-18 : 1 increases cis-9, trans-11-conjugated linoleic acid in human immune cells, but without effects on biomarkers of immune function and inflammation." British Journal of Nutrition 97, no. 6 (2007): 1196–205. http://dx.doi.org/10.1017/s0007114507685183.

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Trans-fatty acid intake is associated with an increased risk of CHD and diabetes. The effects of single trans-fatty acid isomers are largely unexplored. The present study examined the effects of a 6-week supplementation with two trans-18 : 1 isomers (trans-11 and trans-12) in human subjects on immune cells, several inflammatory and immunological biomarkers (for example, IL, TNFα, C-reactive protein, adiponectin, intercellular adhesion molecule-1, prostacyclin, phagocytic process). Following a 2-week adaptation period without supplements, the test group (n 12) received vaccenic acid (trans-11-1
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13

Cook, H. W., and E. A. Emken. "Geometric and positional fatty acid isomers interact differently with desaturation and elongation of linoleic and linolenic acids in cultured glioma cells." Biochemistry and Cell Biology 68, no. 3 (1990): 653–60. http://dx.doi.org/10.1139/o90-094.

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A range of geometrical and positional isomers of 18-carbon acyl chains are potential components of diets containing fats from processed vegetable oils or ruminant animals. We have examined seventeen 18-carbon fatty acids, with cis or trans double bonds in positions 8–15, for their effects on desaturation and chain elongation of [1-14C]18:2(n − 6) or [1-14C]18:3(n − 3), primarily to 20:4(n − 6) or 20:5(n − 3) respectively, in cultured glioma cells. All trans monoenoic positional isomers inhibited Δ5 desaturation by approximately 60% when 18:2(n − 6) was the substrate, with no positional discrim
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14

Newbold, J. R., K. L. Robertshaw, and H. W. Morris. "Associations between concentrations of fat and intermediates of ruminal biohydrogenation in milk of dairy cows." Proceedings of the British Society of Animal Science 1998 (1998): 224. http://dx.doi.org/10.1017/s1752756200598767.

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Rumen bacteria convert polyunsaturated C18 fatty acids to stearic acid (CI8:0) by biohydrogenation. Intermediates include a family of cis/trans isomers of linoleic acid known as conjugated linoleic acid (CLA) and a family of trans isomers of oleic acid (C18:1), chiefly trans-11 C18:1. Trans fatty acids inhibit milk fat synthesis in cows, an effect which Griinari et al. (1997) attributed specifically to trans-10 C18:1. Biohydrogenation intermediates can be incorporated directly into milk. In humans, trans C18:1 in hydrogenated vegetable oil (chiefly trans-9 C18:1) mimic saturated fatty acids as
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15

Newbold, J. R., K. L. Robertshaw, and H. W. Morris. "Associations between concentrations of fat and intermediates of ruminal biohydrogenation in milk of dairy cows." Proceedings of the British Society of Animal Science 1998 (1998): 224. http://dx.doi.org/10.1017/s0308229600034371.

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Rumen bacteria convert polyunsaturated C18 fatty acids to stearic acid (CI8:0) by biohydrogenation. Intermediates include a family of cis/trans isomers of linoleic acid known as conjugated linoleic acid (CLA) and a family of trans isomers of oleic acid (C18:1), chiefly trans-11 C18:1. Trans fatty acids inhibit milk fat synthesis in cows, an effect which Griinari et al. (1997) attributed specifically to trans-10 C18:1. Biohydrogenation intermediates can be incorporated directly into milk. In humans, trans C18:1 in hydrogenated vegetable oil (chiefly trans-9 C18:1) mimic saturated fatty acids as
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16

Delmonte, Pierluigi, Martin P. Yurawecz, Magdi M. Mossoba, Cristina Cruz-Hernandez, and John K. G. Kramer. "Improved Identification of Conjugated Linoleic Acid Isomers Using Silver-Ion HPLC Separations." Journal of AOAC INTERNATIONAL 87, no. 2 (2004): 563–68. http://dx.doi.org/10.1093/jaoac/87.2.563.

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Abstract Silver-ion high-performance liquid chromatography (Ag+-HPLC) has been shown to be effective in the resolution of most of the isomers of conjugated octadecadienoic acids (18:2), also known as conjugated linoleic acid (CLA). The CLA isomers identified in natural fats from ruminants are a mixture of numerous positional and geometric isomers from 7,9- to 12,14–18:2. Ag+-HPLC separates both geometric (trans,trans < cis/trans < cis,cis) and positional CLA isomers using the mobile phase hexane/acetonitrile (99.9:0.1). The elution volumes for the CLA isomers were not only affect
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17

Guckert, James B., David B. Ringelberg, and David C. White. "Biosynthesis of trans fatty acids from acetate in the bacterium Pseudomonas atlantica." Canadian Journal of Microbiology 33, no. 9 (1987): 748–54. http://dx.doi.org/10.1139/m87-129.

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The cis configuration in fatty acids is the only known product of bacterial biosynthetic pathways of monounsaturated membrane fatty acids. The trans configuration is considered "nonphysiologic" or "unnatural." This research shows that in vivo synthesis of trans-monounsaturated membrane fatty acids from acetate occurs in the bacterium Pseudomonas atlantica. The saturated, trans, and cis phospholipid fatty acids (PLFA) of P. atlantica grown in the presence of [14C]acetate were physically separated as the corresponding fatty acid methyl esters (FAME) and the presence of trans FAME was verified by
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18

Ratnayake, W. M. N., J. M. Chardigny, R. L. Wolff, C. C. Bayard, J. L. Sébédio, and L. Martine. "Essential Fatty Acids and Their Trans Geometrical Isomers in Powdered and Liquid Infant Formulas Sold in Canada." Journal of Pediatric Gastroenterology and Nutrition 25, no. 4 (1997): 400–407. http://dx.doi.org/10.1002/j.1536-4801.1997.tb01777.x.

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BackgroundAnimal and human studies have suggested that trans fatty acids might alter some physiological functions and adversely affect the growth and essential fatty acid balance of infants. In this context it is important to know the fatty acid composition, including the levels of trans isomers of oleic, linoleic and α‐linolenic acids in infant formulas.MethodsTen liquid and fourteen powdered formulas for term infants were purchased from retail stores in Canada. The fatty acid composition of each formula was determined by capillary gas‐liquid chromatography.ResultsAll the formulas met the mim
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19

Cruz-Hernandez, Cristina, Zeyuan Deng, Jianqiang Zhou, et al. "Methods for Analysis of Conjugated Linoleic Acids and trans-18:1 Isomers in Dairy Fats by Using a Combination of Gas Chromatography, Silver-Ion Thin-Layer Chromatography/Gas Chromatography, and Silver-Ion Liquid Chromatography." Journal of AOAC INTERNATIONAL 87, no. 2 (2004): 545–62. http://dx.doi.org/10.1093/jaoac/87.2.545.

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Abstract Conjugated linoleic acids (CLA) are octadecadienoic acids (18:2) that have a conjugated double-bond system. Interest in these compounds has expanded since CLA were found to be associated with a number of physiological and pathological responses such as cancer, metastases, atherosclerosis, diabetes, immunity, and body fat/protein composition. The main sources of these conjugated fatty acids are dairy fats. Rumen bacteria convert polyunsaturated fatty acids, especially linoleic and linolenic acids, to CLA and numerous trans- containing mono- and diunsaturated fatty acids. It has been es
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20

Watanabe, Akihiro, and Yasushi Numata. "Determination of the Activation Energy of the Thermal Isomerization of Oleic Acid with Raman Spectroscopy and Partial Least Squares Regression." Spectroscopy Journal 3, no. 2 (2025): 17. https://doi.org/10.3390/spectroscj3020017.

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Unsaturated fatty acids have cis and trans isomers. The naturally stable isomer is the cis isomer, which is changed to the trans isomer by a thermal reaction. The reaction order, reaction constant, and activation energy are required to confirm the reaction mechanism. Therefore, the concentrations of the cis and trans isomers must be determined simultaneously. In the present study, oleic acid (cis isomer) and elaidic acid (trans isomer) were measured using Raman spectroscopy and partial least squares regression. The thermal reaction of oleic acid was performed at several temperatures. The react
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21

Aldai, N., M. E. R. Dugan, D. C. Rolland, and J. K. G. Kramer. "Survey of the fatty acid composition of Canadian beef: Backfat and longissimus lumborum muscle." Canadian Journal of Animal Science 89, no. 3 (2009): 315–29. http://dx.doi.org/10.4141/cjas08126.

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A survey of Canadian retail beef was undertaken with emphasis on the trans fatty acid (TFA) and conjugated linoleic acid (CLA) isomers, and compared with current health recommendations. Thirty striploin steaks were collected in the winter and summer from major grocery stores in Calgary (Alberta, Canada). Steak fatty acid compositions (backfat and longissimus lumborum muscle analysed separately) showed minor seasonal differences with lower total saturates (P < 0.05) and higher total monounsaturates (P < 0.01) in winter, but no differences in total polyunsaturated fatty acids. The ratio of
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22

KU, KUO-LONG, JUI-LONG CHIOU, FANG-CHI LIU, and ROBIN Y. Y. CHIOU. "Advanced Gas Chromatographic–Mass Spectrometric Studies for Identification of the Cellular Octadecenoate Isomers and Changes of Fatty Acid Composition Induced by Ethanol Stress in Escherichia coli and Escherichia coli O157:H7." Journal of Food Protection 70, no. 3 (2007): 616–22. http://dx.doi.org/10.4315/0362-028x-70.3.616.

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Ethanol can be introduced to foods of various origins and is commonly used for surface disinfection. Low concentrations of residual ethanol may provide an opportunity for pathogens to adapt and grow. Change of cellular fatty acid composition is one of adaptation mechanisms enabling bacteria to grow under varied stresses. Since instrumental analyses of bacterial octadecenoate isomers are sophisticated, gas chromatographic analyses of the isomers, namely trans-9-octadecenoate, trans-11-octadecenoate, cis-9-octadecenoate, and cis-11-octadecenoate, and ethanol-induced formation of trans-9-octadece
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23

Murray Skeaff, C., and Sonya Gowans. "Home use of margarine is an important determinant of plasma trans fatty acid status: a biomarker study." British Journal of Nutrition 96, no. 2 (2006): 377–83. http://dx.doi.org/10.1079/bjn20061737.

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The contribution of the home use of margarines, made with partially hydrogenated vegetables oils, to total trans fatty acid intake is difficult to determine using dietary assessment because food composition databases are incomplete for trans fatty acids; moreover, hidden fats in manufactured foods may be the predominant sources of trans fatty acids. The objective of our study was to determine, using plasma phospholipid trans fatty acid composition as a surrogate measure of exposure, whether the home use of margarine or butter is an important determinant of trans fatty acid status. We conducted
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24

Polidori, P., G. L. Maggi, V. M. Moretti, F. Valfrè, and P. Navarotto. "A note on the effect of use of bovine somatotropin on the fatty acid composition of the milk fat in dairy cows." Animal Science 57, no. 2 (1993): 319–22. http://dx.doi.org/10.1017/s0003356100006942.

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Commencing on day 100 ± 10 of lactation, 10 Italian Friesian cows averaging 32·6 kg milk per day were injected biweekly with recombinant bovine somatotropin (BST), while 10 other cows averaging 33-4 kg milk per day were injected with a placebo. Cows were offered ad libitum a total mixed diet throughout the lactation. The experiment lasted 16 weeks; milk samples were taken before and 16, 62 and 91 days after the commencement of BST injection. The objective was to determine the impact of biweekly injections of BST on the synthesis and proportion of milk fatty acids, particularly on cis and trans
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25

Tsuzuki, Wakako. "Inconsistencies in a Highly Polar Capillary Gas Chromatography Column and Necessity of Column Performance Checks for trans Fatty Acid Measurement." Journal of AOAC INTERNATIONAL 95, no. 6 (2012): 1740–43. http://dx.doi.org/10.5740/jaoacint.11-352.

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Abstract GC with a capillary column (60–100 m length) is widely used to measure trans fatty acids in dietary fats and biological tissues. Recently, we have occasionally observed that isothermal operation of an SP-2560 column at 180°C results in incomplete separation of gondoic acid (11c-20:1) and one of the geometric isomers of α-linolenic acid (9t,12c,15c-18:3), although it has been known to produce their baseline separation in American Oil Chemists' Society Official Method Ce 1h-05, as well as in previous studies. Thus, trans isomer (9t,12c,15c-18:3) is one of the main components of trans fa
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Shantha, Nalur Chandrasekaran, Eric Andrew Decker, and Bernhard Hennig. "Comparison of Methylation Methods for the Quantitation of Conjugated Linoleic Acid Isomers." Journal of AOAC INTERNATIONAL 76, no. 3 (1993): 644–49. http://dx.doi.org/10.1093/jaoac/76.3.644.

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Abstract Four methylation methods were evaluated for use in the gas chromatographic (GC) quantitation of conjugated linoleic acid (CLA) isomers, which are potential anticarcinogen. The methods were (1) sodium methoxide in methanol (NaOMe-MeOH), (2) American Oil Chemists' Society (AOCS) procedure Ce 2-66, which involves methanolic sodium hydroxide followed by boron trifluoride in methanol, (3) tetramethylguanidine in methanol (TMG-MeOH), and (4) direct transesterification with methanolbenzene- acetyl chloride (DAC). Purified methyl esters of isomerized linoleic acid containing 86% CLA isomers w
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27

Miklavčič, Milena Bučar, Fouad Taous, Vasilij Valenčič, et al. "Fatty Acid Composition of Cosmetic Argan Oil: Provenience and Authenticity Criteria." Molecules 25, no. 18 (2020): 4080. http://dx.doi.org/10.3390/molecules25184080.

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In this work, fatty-acid profiles, including trans fatty acids, in combination with chemometric tools, were applied as a determinant of purity (i.e., adulteration) and provenance (i.e., geographical origin) of cosmetic grade argan oil collected from different regions of Morocco in 2017. The fatty acid profiles obtained by gas chromatography (GC) showed that oleic acid (C18:1) is the most abundant fatty acid, followed by linoleic acid (C18:2) and palmitic acid (C16:0). The content of trans-oleic and trans-linoleic isomers was between 0.02% and 0.03%, while trans-linolenic isomers were between 0
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Serikov, Maksat, Meruyet Nurgaliyeva, Karima Myrzabek, Maxat Toishimanov, and Farida Baktybayeva. "Defining the Composition of Fat Phase in Foods." Food Processing: Techniques and Technology 52, no. 4 (2022): 685–93. http://dx.doi.org/10.21603/2074-9414-2022-4-2398.

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Trans-fatty acids enter the human body with hydrogenated fats. The oil and fat industry must inform consumers about the fatty acid composition of food products, including the content of saturated fatty acids and trans-isomers. This study used the method of instrumental analysis to determine the fatty-acid profile and the content of trans-fatty acids in margarine.
 The research involved ten commercial samples of margarine. The fatty acid composition was studied by gas chromatography and mass spectrometry. The calibration graphs were based on standard mixes of methyl esters of individual fa
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Luna, Pilar, Javier Fontecha, Manuela Juárez, and Miguel Angel de la Fuente. "Conjugated linoleic acid in ewe milk fat." Journal of Dairy Research 72, no. 4 (2005): 415–24. http://dx.doi.org/10.1017/s0022029905001032.

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Ewe milk fat from five different herds was studied to determine the content of conjugated linoleic acid (CLA) isomers. Research was carried out by combining gas chromatography-mass spectrometry (GC-MS) of fatty acid methyl esters (FAME) and 4,4-dimethyloxazolyne derivatives (DMOX) with silver ion-high performance liquid chromatography (Ag+-HPLC). Reconstructed mass spectral profiles of CLA characteristic ions from DMOX were used to identify positional isomers and Ag+-HPLC to quantify them. Total CLA content varied from 0·57 to 0·97 g/100 g of total fatty acids. FAME and DMOX were separated int
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30

Smith, Brennan K., Lindsay E. Robinson, Robert Nam, and David W. L. Ma. "Trans-fatty acids and cancer: a mini-review." British Journal of Nutrition 102, no. 9 (2009): 1254–66. http://dx.doi.org/10.1017/s0007114509991437.

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The association between trans-fatty acids (TFA) and cancer risk is poorly understood and remains controversial. It is recognised that unique biological effects are associated with specific isoforms within families of fatty acids such as those belonging to the n-3 fatty acids. Furthermore, the interactions between diet and genetic polymorphisms are increasingly recognised for their potential risk-modifying effects on human health and disease. Therefore, the aim of the present review is to evaluate whether specific TFA isomers and genetic polymorphisms differentially modify cancer risk in prosta
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Mossoba, Magdi M., Martin P. Yurawecz, Pierluigi Delmonte, and John K. G. Kramer. "Overview of Infrared Methodologies for trans Fat Determination." Journal of AOAC INTERNATIONAL 87, no. 2 (2004): 540–44. http://dx.doi.org/10.1093/jaoac/87.2.540.

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Abstract trans Fatty acids are present in a variety of foods like dairy products, but the major sources are products that contain commercially hydrogenated fats. Some studies have shown that trans fatty acids elevate levels of serum low-density lipoprotein (LDL)-cholesterol and lower high-density lipoprotein (HDL)-cholesterol. The quantitation and identification of trans fatty acid isomers is difficult because of the wide range of positional monoene, diene, and triene fatty acid isomers present in hydrogenated oils. This is complicated by the cis positional isomers that are also present, as we
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Tappia, Paramjit S., Rabban Mangat, Cindy Gabriel, Melissa R. Dent, Nina Aroutiounova, and Hope Weiler. "Gender differences in the cardiac response to dietary conjugated linoleic acid isomers." Canadian Journal of Physiology and Pharmacology 84, no. 2 (2006): 257–64. http://dx.doi.org/10.1139/y05-149.

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The present study was undertaken to assess the heart function, by the in vivo catheterization technique, of healthy male and female Sprague–Dawley rats fed different conjugated linoleic acid (CLA) isomers, (cis-9, trans-11 (c9,t11) and trans-10, cis-12 (t10,c12)) individually and in combination (50:50 mix as triglyceride or fatty acids) from 4 to 20 weeks of age. Whereas the triglyceride form of the CLA isomer mix lowered the heart rate, the rate of contraction (+dP/dt) and rate of relaxation (–dP/dt), systolic and diastolic pressures, mean arterial pressure, and the left ventricular systolic
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Mir, P. S., M. Ivan, M. L. He, et al. "Dietary manipulation to increase conjugated linoleic acids and other desirable fatty acids in beef: A review." Canadian Journal of Animal Science 83, no. 4 (2003): 673–85. http://dx.doi.org/10.4141/a03-002.

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The diet is the source of many essential fatty acids such as linoleic and linolenic acids for all mammals. These fatty acids either, as altered isomers or as other elongated products, have been found to provide unique advantages to human health. Currently two conjugated linoleic acids (CLA) isomers (cis-9, trans-11 C18:2; trans-10, cis-12 C18:2) and two elongated products of linolenic acid [eicosapentaenoic acid (EPA, C20:5 n-3), docosahexaenoic acid (DHA, C22:6 n-3)] have been recognized for their roles in maintaining human health. Consumers can obtain these functional fatty acids from beef i
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Chubarova, A. D., M. S. Turchaninova, N. V. Gogadze, E. A. Vilms, and E. V. Shcherba. "Hygienic assessment of the consumption of trans fatty acids by the population of the Omsk region." Fundamental and Clinical Medicine 8, no. 4 (2024): 65–72. http://dx.doi.org/10.23946/2500-0764-2023-8-4-65-72.

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Aim. Assessment of the content of trans-fatty acids in foods consumed by the population of the Omsk region and the values of their actual consumption.Materials and Methods. The results of laboratory studies of food products from the following groups were analyzed: fast food (hamburgers, french fries, chips); confectionery; oils, fats; meat products; dairy products (n=438). In product samples, the mass fraction of trans-fatty acid isomers was determined by gas chromatography. An assessment was made of the actual nutrition and consumption of trans fats by the inhabitants of the region using the
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Mossoba, Magdi M., Julie Moss, and John K. G. Kramer. "Trans Fat Labeling and Levels in U.S. Foods: Assessment of Gas Chromatographic and Infrared Spectroscopic Techniques for Regulatory Compliance." Journal of AOAC INTERNATIONAL 92, no. 5 (2009): 1284–300. http://dx.doi.org/10.1093/jaoac/92.5.1284.

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Abstract Trans fatty acids are found in a variety of foods like dairy and meat products, but the major dietary sources are products that contain commercially hydrogenated fats. There has been a renewed need for accurate analytical methods for the quantitation of total Trans fat since mandatory requirements to declare the amount of Trans fat present in food products and dietary supplements were issued in many countries. Official capillary GC and IR methodologies are the two most common validated methods used to identify and quantify Trans fatty acids for regulatory compliance. The present artic
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Osipov, M. V. "Impact of raw materials on the nutritional value of confections." Tovaroved prodovolstvennykh tovarov (Commodity specialist of food products), no. 12 (December 5, 2024): 741–44. https://doi.org/10.33920/igt-01-2412-09.

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The techniques for determining the nutritional value of confectionery products, namely, determining the mass fraction of protein, fat, water-soluble vitamins B1 and B2, omega-3 fatty acids, and cholesterol in fat-containing confectionery products, have been developed. Data on the content of protein, fat, saturated fatty acids and trans isomers of unsaturated fatty acids, B vitamins, omega-3 fatty acids,and cholesterol in fat-containing confectionery products have been obtained. A methodology to monitor the content of vitamins, omega-3 fatty acids, and cholesterol in fat-containing confectioner
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Ratnayake, W. M. Nimal, Louise J. Plouffe, Elodie Pasquier, and Claude Gagnon. "Temperature-Sensitive Resolution of cis- and trans-Fatty Acid Isomers of Partially Hydrogenated Vegetable Oils on SP-2560 and CP-Sil 88 Capillary Columns." Journal of AOAC INTERNATIONAL 85, no. 5 (2002): 1112–18. http://dx.doi.org/10.1093/jaoac/85.5.1112.

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Abstract This study examines the effect of the column operating temperature of 100 m SP-2560 and CP-Sil 88 capillary gas chromatographic (GC) columns on the separation of cis- and trans-octadecenoic (18:1) isomers in partially hydrogenated vegetable oils. The overlapping GC peaks were measured at column isothermal temperatures of 170, 175, 180, 185, and 190°C. With both columns, isothermal operation at 180°C produced the fewest overlapping peaks of the cis and trans isomers. At this temperature, all trans-18:1 isomers, except 13t-18:1 (t = trans), 14t-18:1, and 15t-18:1 isomers were resolved f
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38

Artiga, Aracely, Xavier Ortiz Almirall, Francesc Broto Puig, and Jordi Díaz-Ferrero. "Development of a GC-FID method for the analysis of fatty acids in palm olein to study the formation of C18:1 trans during deep discontinuous frying processes in El Salvador food industry." Afinidad. Journal of Chemical Engineering Theoretical and Applied Chemistry 81, no. 603 (2024): 218–28. http://dx.doi.org/10.55815/430521.

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An analytical method for the derivatization of fatty acids to methyl esters using basic catalysis with special emphasis on the identification of the different C18:1 trans isomers was developed and validated, showing excellent accuracy, precision and linearity. This method was employed to evaluate the degradation of palm olein during the deep discontinuous frying process of potatoes and bananas over 60 hours at different temperatures, simulating most habitual food processing methods used in El Salvador. It was observed that untreated oils already showed low levels of trans fatty acids (around 0
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Kravic, Snezana, Zvonimir Suturovic, Jaroslava Svarc-Gajic, Zorica Stojanovic, Mira Pucarevic, and Ivana Nikolic. "Fatty acid composition including trans isomers of Serbian biscuits." Chemical Industry 65, no. 2 (2011): 139–46. http://dx.doi.org/10.2298/hemind101001078k.

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An experimental study was carried out with the aim of evaluating the quality of the lipid fraction of Serbian biscuits. Total fat contents of the biscuit samples ranged between 10.2% and 24.5%. The saturated, cis-monounsaturated and cis-polyunsaturated fatty acid contents were within the ranges of 18.585.6%, 10.649.9% and 2.713.3% of total fatty acids, respectively. The content of trans fatty acids (TFA) ranged from 0.0% to 42.5% and the mean was 10.2%. In a total of 34 investigated samples, 10 of them were found to be trans - free, 8 contained low level of TFA (under 2%), 4 samples contained
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40

N, Erdenechimeg, Odonchimeg M, Oyundari M, and Bayarmaa B. "Determination of trans-isomers of unsaturated Fatty acids in foods by FTIR." Bulletin of the Institute of Chemistry and Chemical Technology, Mongolian Academy of Sciences, no. 7 (February 6, 2020): 49–53. http://dx.doi.org/10.5564/bicct.v0i7.1273.

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It has been proven that the trans-isomers of unsaturated fatty acids that are formed during the hydrogenation of vegetable oils have negative effects on human health. Therefore, it is important to control the content of trans fatty acids in foodstuffs. At present in Mongolia, there are no national policies on controlling and regulating the content of trans fatty acids in food. Our country imports 20 - 25 tones of animals and vegetable oils, annually. Since we have not had regulations for trans fatty acids content in the foodstuffs, their content is regulated or limited by the legislation of th
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Miwa, Hiroshi, and Magobei Yamamoto. "Rapid Liquid Chromatographic Determination of Fatty Acids as 2-Nitrophenylhydrazine Derivatives." Journal of AOAC INTERNATIONAL 79, no. 2 (1996): 493–97. http://dx.doi.org/10.1093/jaoac/79.2.493.

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Abstract Direct derivatization of saponified fats and oils with 2-nitrophenylhydrazine hydrochloride produced corresponding fatty acid hydrazides without timeconsuming sample cleanup and/or extraction steps. Hydrazides were injected directly into a liquid chromatograph. Improved isocratic separation was achieved within only 22 min for 29 saturated and mono- and polyunsaturated fatty acids (C8:0- C22:6), including cis-trans isomers and doublebond positional isomers. The technique has good quantitative accuracy while reducing analysis time, cost, and labor.
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42

Roy, Uzzal, Robert Joshua, Russell L. Stark, and Michael Balazy. "Cytochrome P450/NADPH-dependent biosynthesis of 5,6-trans-epoxyeicosatrienoic acid from 5,6-trans-arachidonic acid." Biochemical Journal 390, no. 3 (2005): 719–27. http://dx.doi.org/10.1042/bj20050681.

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5,6-trans-AA (5,6-TAA, where TAA stands for trans-arachidonic acid) is a recently identified trans fatty acid that originates from the cis–trans isomerization of AA initiated by the NO2 radical. This trans fatty acid has been detected in blood circulation and we suggested that it functions as a lipid mediator of the toxic effects of NO2. To understand its role as a lipid mediator, we studied the metabolism of 5,6-TAA by liver microsomes stimulated with NADPH. Profiling of metabolites by liquid chromatography/MS revealed a complex mixture of oxidized products among which were four epoxides, the
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43

Przybylski, Roman, and Felix A. Aladedunye. "Formation of Trans Fats: During Food Preparation." Canadian Journal of Dietetic Practice and Research 73, no. 2 (2012): 98–101. http://dx.doi.org/10.3148/73.2.2012.98.

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Purpose: An investigation was completed to determine how typical cooking procedures used in food preparation, such as baking and stir-frying, affect trans fats formation. Methods: Canola oil was used as the main fat ingredient. Zucchini cake and gingersnap cookies were baked at 180° C and 200° C, while stir-fried chicken was prepared at 200° C and 275° C. The lipids from the food were extracted following the Folch procedure, and analyzed for trans fatty acids according to ISO official method 15304. Results: Minimal changes were observed in the amount of trans fats during baking. Application of
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44

Paszczyk, Beata, and Joanna Łuczyńska. "The Comparison of Fatty Acid Composition and Lipid Quality Indices in Hard Cow, Sheep, and Goat Cheeses." Foods 9, no. 11 (2020): 1667. http://dx.doi.org/10.3390/foods9111667.

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This study aimed to evaluate the fatty acid composition, including the contents, of conjugated linoleic acid cis9trans11 C18:2 (CLA) and trans C18:1 and C18:2 isomers in hard cow, sheep, and goat cheeses found on the Polish market and to compare lipid quality indices in these cheeses. The gas chromatography method was used to determine the fatty acid profile. The study demonstrated various contents of cis9trans 11 C18:2 (CLA), trans C18:1, and C18:2 isomers and the values of lipid quality indices in the cheeses. Sheep and goat cheeses were richer sources of short-chain fatty acids (SCFA) (14.7
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45

Martínez Marín, Andrés L., Pilar Gómez-Cortés, Nieves Núñez Sánchez, et al. "Associations between major fatty acids in plant oils fed to dairy goats and C18 isomers in milk fat." Journal of Dairy Research 82, no. 2 (2015): 152–60. http://dx.doi.org/10.1017/s002202991500014x.

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Relationships between fatty acids (FAs) in plant oils included in goat diets and milk fat C18 isomers were determined by Principal Factor Analysis (PFA). The three first principal factors (PF1, PF2 and PF3) accounted for 64·5% of the total variation in milk FAs contents. Fatty acids with a double bond at carbons 13, 14, 15 or 16 had high (>0·6) and positive loadings for PF1, trans-4 to trans-8 C18:1 for PF2, whereas trans-10 C18:1, trans-11 C18:1 and cis-9 trans-11 C18:2 showed high and positive loadings for PF3. Pearson's correlations supported that PF1, PF2 and PF3 were related to α-linol
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Терещук, Любовь, Lyubovʼ Tereshchuk, Ксения Старовойтова, and Ksenia Starovoytova. "Hghly Effective Methods of Modification of Fats in Milk-Containing Products." Food Processing: Techniques and Technology 48, no. 3 (2019): 115–23. http://dx.doi.org/10.21603/2074-9414-2018-3-115-123.

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The resources of natural solid fats that used in various branches of the food industry are limited. As a result, they are often substituted by chemically and physically modified fats. Until recently, hydrogenation was the main method of modification of fats. Modification allows for products with a high hardness and sufficient resilience to oxidation due to a large amount of trans-isomers. Since the legislation in the sphere of food industry is changing, producers have to look for new solutions for fat-containing products that would correspond with the new restrictions for trans-isomers of fatt
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47

Kravchuk, O. P., G. M. Balan, A. V. Koval, T. V. Adamchuk, T. V. Yevtushenko, and M. V. Hreben. "Current status of legislative regulation of the content of trans-isomers of fatty acids in food products in Ukraine and worldwide (review)." One Health and Nutrition Problems of Ukraine 60, no. 1 (2024): 23–36. http://dx.doi.org/10.33273/2663-9726-2024-60-1-23-36.

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Abstract. The influence of fats and oils on human health is in constant attention of leading experts in the field of public health, of both national and international institutions. This is especially true concerning industrially obtained trans fats and their regulation. Currently, in Ukraine, the requirements for the content of trans fatty acids in food products are regulated by documents of two levels. Firstly, the requirements for the content of trans fatty acids in certain types of oil and fat products are set out in the national standards (DSTU), which are do not cover the entire range of
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48

Dunshea, F. R., G. P. Walker, E. Ostrowska, and P. T. Doyle. "Seasonal variation in the concentrations of conjugated linoleic and trans fatty acids in milk fat from commercial dairy farms is associated with pasture and grazing management and supplementary feeding practices." Australian Journal of Experimental Agriculture 48, no. 8 (2008): 1062. http://dx.doi.org/10.1071/ea07286.

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A study of irrigated pasture-based dairy farms that used split calving (autumn and spring) was undertaken in northern Victoria, Australia, to examine associations between nutrition, time of year and season of calving on the concentrations of isomers of trans 18 : 1 fatty acids and conjugated linoleic acids (CLA) in milk fat. Factors associated with time of year explained most of the variation, with the highest concentrations observed in spring and summer when pasture intake by herds was high. However, there was substantial variation observed between herds and time of year. The mean total CLA c
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49

Jalc, D., M. Certik, K. Kundrikova, and P. Namestkova. "Effect of unsaturated C18 fatty acids (oleic, linoleic and α-linolenic acids) on ruminal fermentation and production of fatty acids isomers in artificial rumen." Veterinární Medicína 52, No. 3 (2008): 87–94. http://dx.doi.org/10.17221/2012-vetmed.

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The objective of this study was to examine the effect of oleic (OA), linoleic (LA) and α-linolenic (ALA) acid used as supplements (3.5% wt/wt) to a diet containing 80% lucerne and 20% barley on rumen fermentation and lipid metabolism in an artificial rumen (Rusitec). The experiment lasted 12 days with 6 days of stabilization period. The fatty acid (FA) supplementation to a mixed diet did not affect any parameters of rumen fermentation (degradation of DM, NDF, ADF, total VFA production, production of acetate, propionate and butyrate). The methane production was decreased numerically (
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50

Sobhi, Hany F., Xinjie Zhao, Peter Plomgaard, et al. "Identification and regulation of the xenometabolite derivatives cis- and trans-3,4-methylene-heptanoylcarnitine in plasma and skeletal muscle of exercising humans." American Journal of Physiology-Endocrinology and Metabolism 318, no. 5 (2020): E701—E709. http://dx.doi.org/10.1152/ajpendo.00510.2019.

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Little is known about xenometabolites in human metabolism, particularly under exercising conditions. Previously, an exercise-modifiable, likely xenometabolite derivative, cis-3,4-methylene-heptanoylcarnitine, was reported in human plasma. Here, we identified trans-3,4-methylene-heptanoylcarnitine, and its cis-isomer, in plasma and skeletal muscle by liquid chromatography-mass spectrometry. We analyzed the regulation by exercise and the arterial-to-venous differences of these cyclopropane ring-containing carnitine esters over the hepatosplanchnic bed and the exercising leg in plasma samples obt
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