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1

Williams, Reg Arthur, Bonnie M. Hagerty, and Geneal Brooks. "Trier Social Stress Test." Nursing Research 53, no. 4 (2004): 277–80. http://dx.doi.org/10.1097/00006199-200407000-00011.

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Vors, Olivier, Tanguy Marqueste, and Nicolas Mascret. "The Trier Social Stress Test and the Trier Social Stress Test for groups: Qualitative investigations." PLOS ONE 13, no. 4 (2018): e0195722. http://dx.doi.org/10.1371/journal.pone.0195722.

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3

Krishnaveni, G. V., S. R. Veena, A. Jones, et al. "Trier social stress test in Indian adolescents." Indian Pediatrics 51, no. 6 (2014): 463–67. http://dx.doi.org/10.1007/s13312-014-0437-5.

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4

Dahlman, Anna Sjors, Jenny Hadrévi, and Ingibjorg Jonsdottir. "Metabolic responses to the Trier Social Stress Test." Psychoneuroendocrinology 100 (February 2019): S16—S17. http://dx.doi.org/10.1016/j.psyneuen.2018.12.068.

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5

Allen, Andrew P., Paul J. Kennedy, Samantha Dockray, John F. Cryan, Timothy G. Dinan, and Gerard Clarke. "The Trier Social Stress Test: Principles and practice." Neurobiology of Stress 6 (February 2017): 113–26. http://dx.doi.org/10.1016/j.ynstr.2016.11.001.

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6

Fallon, Monica A., Jesus Serrano Careaga, David A. Sbarra, and Mary-Frances OʼConnor. "Utility of a Virtual Trier Social Stress Test." Psychosomatic Medicine 78, no. 7 (2016): 835–40. http://dx.doi.org/10.1097/psy.0000000000000338.

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7

Hellhammer⁎, J., and M. Schubert. "Instantaneous stress perception (ISP) during the Trier Social Stress Test (TSST)." European Journal of Pharmacology 668 (September 2011): e45. http://dx.doi.org/10.1016/j.ejphar.2011.09.303.

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8

Miller, Melissa, Madeline Divine, Ciara McAfee, et al. "A Semi‐Virtual Trier Social Stress Test (SV-TSST)." Psychoneuroendocrinology 172 (February 2025): 107267. https://doi.org/10.1016/j.psyneuen.2024.107267.

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9

Tyrka, A. R., L. M. Wier, G. M. Anderson, C. W. Wilkinson, L. H. Price, and L. L. Carpenter. "Temperament and response to the Trier Social Stress Test." Acta Psychiatrica Scandinavica 115, no. 5 (2007): 395–402. http://dx.doi.org/10.1111/j.1600-0447.2006.00941.x.

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10

Karlsson, Kåre. "Stress Response in Swedish Ambulance Personnel Evaluated by Trier Social Stress Test." Journal of Health and Environmental Research 5, no. 1 (2019): 14. http://dx.doi.org/10.11648/j.jher.20190501.13.

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11

Herhaus, Benedict, and Katja Petrowski. "Cortisol Stress Reactivity to the Trier Social Stress Test in Obese Adults." Obesity Facts 11, no. 6 (2018): 491–500. http://dx.doi.org/10.1159/000493533.

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12

Urban-Wojcik, Emily J., Susan T. Charles, and Linda J. Levine. "Modifying the Trier Social Stress Test to Induce Positive Affect." Affective Science 2, no. 4 (2021): 427–37. http://dx.doi.org/10.1007/s42761-021-00074-6.

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13

Wolf, Oliver T. "Memories of and influenced by the Trier Social Stress Test." Psychoneuroendocrinology 105 (July 2019): 98–104. http://dx.doi.org/10.1016/j.psyneuen.2018.10.031.

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14

Labuschagne, Izelle, Caitlin Grace, Peter Rendell, Gill Terrett, and Markus Heinrichs. "An introductory guide to conducting the Trier Social Stress Test." Neuroscience & Biobehavioral Reviews 107 (December 2019): 686–95. http://dx.doi.org/10.1016/j.neubiorev.2019.09.032.

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15

Maniaci, G., A. E. Goudriaan, C. Cannizzaro, and R. J. van Holst. "Impulsivity and Stress Response in Pathological Gamblers During the Trier Social Stress Test." Journal of Gambling Studies 34, no. 1 (2017): 147–60. http://dx.doi.org/10.1007/s10899-017-9685-3.

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16

YANG, Juan, Yan HOU, Yu YANG, and Qing-Lin ZHANG. "Impact of Trier Social Stress Test (TSST) on Salivary Cortisol Secretion." Acta Psychologica Sinica 43, no. 4 (2012): 403–9. http://dx.doi.org/10.3724/sp.j.1041.2011.00403.

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17

Mihara, Kengo, Hisayoshi Okamura, Yoshihisa Shoji, Kyoko Tashiro, Yukie Kinoshita, and Akira Tsuda. "Personal Growth and Psychobiological Stress Responsiveness to the Trier Social Stress Test in Students." Sustainability 12, no. 11 (2020): 4497. http://dx.doi.org/10.3390/su12114497.

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The current study aimed to examine the effects of personal growth (PG) on psychobiological responses at baseline and responsiveness to laboratory acute stress in students. Twenty-four healthy students were recruited as participants. Participants were screened from 203 candidates according to levels of PG using Ryff’s scale and classified into high and low PG groups. During the laboratory session, 13 high and 11 low PG participants underwent the Trier Social Stress Test. Heart rate and high-frequency (HF) heart rate variability were monitored throughout the experiment. Salivary free-3-methoxy-4
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18

Liu, Qing, and Wenjuan Zhang. "Sex Differences in Stress Reactivity to the Trier Social Stress Test in Virtual Reality." Psychology Research and Behavior Management Volume 13 (October 2020): 859–69. http://dx.doi.org/10.2147/prbm.s268039.

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19

Kirschbaum, Clemens. "Why we need an online version of the Trier Social Stress Test." Psychoneuroendocrinology 125 (March 2021): 105129. http://dx.doi.org/10.1016/j.psyneuen.2021.105129.

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20

Vileisis, Julia, Benjamin Ely, Seunghee Kim-Schulze, et al. "Trier Social Stress Test Does Not Produce a Cytokine Reaction in Adolescence." Biological Psychiatry 89, no. 9 (2021): S378. http://dx.doi.org/10.1016/j.biopsych.2021.02.939.

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21

Rosenbaum, David, Paula Hilsendegen, Mara Thomas, et al. "Cortical hemodynamic changes during the Trier Social Stress Test: An fNIRS study." NeuroImage 171 (May 2018): 107–15. http://dx.doi.org/10.1016/j.neuroimage.2017.12.061.

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22

Hanlin, L. C., J. Price, G. Zhang, N. Assaf, J. R. Mitchell, and N. Rohleder. "Fasting reduces plasma interleukin-6 responses to the trier social stress test." Brain, Behavior, and Immunity 49 (October 2015): e22. http://dx.doi.org/10.1016/j.bbi.2015.06.093.

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23

Hellhammer, J. "The Trier Social Stress Test (TSST) — A valid tool for clinical studies." European Journal of Pharmacology 668 (September 2011): e45-e46. http://dx.doi.org/10.1016/j.ejphar.2011.09.304.

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24

Wallergård, Mattias, Peter Jönsson, Gerd Johansson, and Björn Karlson. "A Virtual Reality Version of the Trier Social Stress Test: A Pilot Study." Presence: Teleoperators and Virtual Environments 20, no. 4 (2011): 325–36. http://dx.doi.org/10.1162/pres_a_00052.

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One of the most common methods of inducing stress in the laboratory in order to examine the stress response in healthy and clinical populations is the Trier Social Stress Test (TSST). Briefly, the participant is asked to deliver a speech and to perform an arithmetic task in front of an evaluating committee. The committee, consisting of three trained actors, does not respond emotionally during the test, which makes the situation very stressful for the participant. One disadvantage of the TSST is that it can be difficult to hold the experimental conditions constant. In particular, it may be diff
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25

Allen, Andrew P., Paul J. Kennedy, John F. Cryan, Timothy G. Dinan, and Gerard Clarke. "Biological and psychological markers of stress in humans: Focus on the Trier Social Stress Test." Neuroscience & Biobehavioral Reviews 38 (January 2014): 94–124. http://dx.doi.org/10.1016/j.neubiorev.2013.11.005.

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26

Kirschbaum, Clemens, Karl-Martin Pirke, and Dirk H. Hellhammer. "The ‘Trier Social Stress Test’ – A Tool for Investigating Psychobiological Stress Responses in a Laboratory Setting." Neuropsychobiology 28, no. 1-2 (1993): 76–81. http://dx.doi.org/10.1159/000119004.

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27

Henze, Gina-Isabelle, Sandra Zänkert, David F. Urschler, et al. "Testing the ecological validity of the Trier Social Stress Test: Association with real-life exam stress." Psychoneuroendocrinology 75 (January 2017): 52–55. http://dx.doi.org/10.1016/j.psyneuen.2016.10.002.

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28

Eagle, David E., Joshua A. Rash, Logan Tice, and Rae Jean Proeschold-Bell. "Evaluation of a remote, internet-delivered version of the Trier Social Stress Test." International Journal of Psychophysiology 165 (July 2021): 137–44. http://dx.doi.org/10.1016/j.ijpsycho.2021.03.009.

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29

Nitsch, Felix Jan, Manuela Sellitto, and Tobias Kalenscher. "Trier social stress test and food-choice: Behavioral, self-report & hormonal data." Data in Brief 37 (August 2021): 107245. http://dx.doi.org/10.1016/j.dib.2021.107245.

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30

Childs, Emma, Lisa Marie Vicini, and Harriet De Wit. "Responses to the Trier Social Stress Test (TSST) in single versus grouped participants." Psychophysiology 43, no. 4 (2006): 366–71. http://dx.doi.org/10.1111/j.1469-8986.2006.00414.x.

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31

Hanlin, Luke, Jonah Price, Ge Zhang, Naomi Assaf, James Mitchell, and Nicolas Rohleder. "Fasting modulates interleukin-6 and cortisol reactivity to the Trier Social Stress Test." Psychoneuroendocrinology 61 (November 2015): 69. http://dx.doi.org/10.1016/j.psyneuen.2015.07.581.

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32

Kelly, Megan M., Audrey R. Tyrka, George M. Anderson, Lawrence H. Price, and Linda L. Carpenter. "Sex differences in emotional and physiological responses to the Trier Social Stress Test." Journal of Behavior Therapy and Experimental Psychiatry 39, no. 1 (2008): 87–98. http://dx.doi.org/10.1016/j.jbtep.2007.02.003.

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33

Bershad, Anya K., Melissa A. Miller, and Harriet de Wit. "MDMA does not alter responses to the Trier Social Stress Test in humans." Psychopharmacology 234, no. 14 (2017): 2159–66. http://dx.doi.org/10.1007/s00213-017-4621-x.

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34

Birkett, Melissa, Lauren Johnson, and Christopher Gelety. "Investigation of Sex Differences In sIgA Response to the Trier Social Stress Test." Stress and Health 33, no. 2 (2016): 158–63. http://dx.doi.org/10.1002/smi.2680.

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35

Preston, D. C., B. J. Sander, and J. L. Gordon. "Comparing In-Person and Internet-Delivered versions of the trier social stress test." Psychoneuroendocrinology 172 (February 2025): 107317. https://doi.org/10.1016/j.psyneuen.2025.107317.

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36

Helminen, Emily C., Melissa L. Morton, Qiu Wang, and Joshua C. Felver. "Stress Reactivity to the Trier Social Stress Test in Traditional and Virtual Environments: A Meta-Analytic Comparison." Psychosomatic Medicine 83, no. 3 (2021): 200–211. http://dx.doi.org/10.1097/psy.0000000000000918.

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37

Pisanski, Katarzyna, Aleksander Kobylarek, Luba Jakubowska, et al. "Multimodal stress detection: Testing for covariation in vocal, hormonal and physiological responses to Trier Social Stress Test." Hormones and Behavior 106 (November 2018): 52–61. http://dx.doi.org/10.1016/j.yhbeh.2018.08.014.

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38

Wolf, Oliver T., and Serkan Het. "Using the Trier Social Stress Test (TSST) in pre-post designs: Two clinical examples." Psychoneuroendocrinology 119 (September 2020): 105019. http://dx.doi.org/10.1016/j.psyneuen.2020.105019.

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39

Fiocco, Alexandra J., Ridha Joober, and Sonia J. Lupien. "Education modulates cortisol reactivity to the Trier Social Stress Test in middle-aged adults." Psychoneuroendocrinology 32, no. 8-10 (2007): 1158–63. http://dx.doi.org/10.1016/j.psyneuen.2007.08.008.

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40

Het, S., N. Rohleder, D. Schoofs, C. Kirschbaum, and O. T. Wolf. "Neuroendocrine and psychometric evaluation of a placebo version of the ‘Trier Social Stress Test’." Psychoneuroendocrinology 34, no. 7 (2009): 1075–86. http://dx.doi.org/10.1016/j.psyneuen.2009.02.008.

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41

Bernhard, Anka, Anne Martinelli, Katharina Ackermann, Inga D. Neumann, Clemens Kirschbaum, and Christine M. Freitag. "Reactivity of oxytocin in the Trier Social Stress Test: A proof of concept study." Psychoneuroendocrinology 61 (November 2015): 73. http://dx.doi.org/10.1016/j.psyneuen.2015.07.592.

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42

Therrien, Fanny, Vicky Drapeau, Josée Lalonde, et al. "Cortisol response to the Trier Social Stress Test in obese and reduced obese individuals." Biological Psychology 84, no. 2 (2010): 325–29. http://dx.doi.org/10.1016/j.biopsycho.2010.03.013.

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43

Shiban, Youssef, Julia Diemer, Simone Brandl, Rebecca Zack, Andreas Mühlberger, and Stefan Wüst. "Trier Social Stress Test in vivo and in virtual reality: Dissociation of response domains." International Journal of Psychophysiology 110 (December 2016): 47–55. http://dx.doi.org/10.1016/j.ijpsycho.2016.10.008.

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44

Viola, Thiago Wendt, Luis Eduardo Wearick-Silva, Saulo Gantes Tractenberg, and Rodrigo Grassi-Oliveira. "Translation and adaptation of the Trier social Stress Test for children into Portuguese language." Temas em Psicologia 22, no. 3 (2014): 655–62. http://dx.doi.org/10.9788/tp2014.3-10.

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45

Montero-López, Eva, Ana Santos-Ruiz, M. Carmen García-Ríos, Raúl Rodríguez-Blázquez, Miguel Pérez-García, and María Isabel Peralta-Ramírez. "A virtual reality approach to the Trier Social Stress Test: Contrasting two distinct protocols." Behavior Research Methods 48, no. 1 (2015): 223–32. http://dx.doi.org/10.3758/s13428-015-0565-4.

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46

Mazurka, Raegan, Katherine E. Wynne-Edwards, and Kate L. Harkness. "Sex Differences in the Cortisol Response to the Trier Social Stress Test in Depressed and Nondepressed Adolescents." Clinical Psychological Science 6, no. 3 (2017): 301–14. http://dx.doi.org/10.1177/2167702617739973.

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Two of the most robust findings in depression research are (a) that women are twice as likely to become depressed than men and (b) that stress is an important risk factor for depression. Although sex differences in stress reactivity may be an important determinant of differential risk for depression, few studies have examined sex differences in neurobiological reactivity to stress. The purpose of the current study was to assess sex differences in the HPA axis response to stress in depressed versus healthy controls by comparing the cortisol response to the Trier Social Stress Test in a communit
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47

Reschke-Hernández, Alaine E., Katrina L. Okerstrom, Angela Bowles Edwards, and Daniel Tranel. "Sex and stress: Men and women show different cortisol responses to psychological stress induced by the Trier social stress test and the Iowa singing social stress test." Journal of Neuroscience Research 95, no. 1-2 (2016): 106–14. http://dx.doi.org/10.1002/jnr.23851.

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48

Hellhammer, Juliane, and Melanie Schubert. "The physiological response to Trier Social Stress Test relates to subjective measures of stress during but not before or after the test." Psychoneuroendocrinology 37, no. 1 (2012): 119–24. http://dx.doi.org/10.1016/j.psyneuen.2011.05.012.

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49

Petrowski, Katja, Benedict Herhaus, Rupert Conrad, and Stefan R. Bornstein. "Hormonal responsiveness in the Trier Social Stress Test and the dexamethasone‐corticotropin releasing hormone test in healthy individuals." PsyCh Journal 10, no. 2 (2021): 305–17. http://dx.doi.org/10.1002/pchj.424.

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50

Sequeira, Isabelle K., Addie S. Longmire, and Naomi J. McKay. "Trier Social Stress Test Elevates Blood Pressure, Heart Rate, and Anxiety, But a Singing Test or Unsolvable Anagrams Only Elevates Heart Rate, among Healthy Young Adults." Psych 3, no. 2 (2021): 171–83. http://dx.doi.org/10.3390/psych3020015.

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The Trier Social Stress Test (TSST) is a psychosocial stressor that effectively stimulates the stress response but is labor and time intensive. Although other psychological stressors are often used experimentally, none are known to comparably elevate stress. Two stressors that may potentially elevate stress are a singing task (ST) and unsolvable anagrams, but there are not enough data to support their effectiveness. In the current experiment, 53 undergraduate males and females (mean age = 21.9 years) were brought into the laboratory, and baseline blood pressure, heart rate, self-rated anxiety,
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