Relevant bibliographies by topics / Unnatural amino acid mutagenesis / Dissertations / Theses
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Dissertations / Theses on the topic 'Unnatural amino acid mutagenesis'

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Published: 4 June 2021
Last updated: 12 February 2022

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1

Mendes, Kimberly Rose Marie. "Catalysis and Regulation of the Allosteric Enzyme Aspartate Transcarbamoylase." Thesis, Boston College, 2010. http://hdl.handle.net/2345/2975.

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Thesis advisor: Evan R. Kantrowitz<br>The understanding of how cells regulate and control all aspects of their function is vital for our ability to intervene when these control mechanisms break down. Almost all modes of cellular regulation can be related in some manner to protein conformational changes such as the quaternary conformational changes of allosteric enzymes that alter enzyme activity to regulate metabolism. The control of metabolic pathways by allosteric enzymes is analogous to a molecular valve with "on" and "off" positions. In the "off" position, flow through the pathway is sever
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2

Poshtiban, Anahita [Verfasser]. "Gating modules of the AMPA receptor pore domain revealed by unnatural amino acid mutagenesis / Anahita Poshtiban." Berlin : Medizinische Fakultät Charité - Universitätsmedizin Berlin, 2020. http://d-nb.info/1218075902/34.

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3

Crane, Peter. "Protein based molecular probes by unnatural amino acid incorporation." Thesis, University of Oxford, 2018. http://ora.ox.ac.uk/objects/uuid:772076fc-00f2-4ca7-bfa9-3da1ce7093cb.

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The "tag & modify" strategy for protein modification relies upon the genetic incorporation of an uncommon or unnatural amino acid into a protein backbone, followed by a chemo-selective modification to yield differentially modified proteins. This thesis describes the creation of a protein-based glycoconjugate tool for interrogating biological function. In Chapter 2, the unnatural amino acid, azidohomoalanine was genetically incorporated into a library of distance defined Np276 proteins via a selective pressure incorporation. Methods to prevent the common post translational modification N-termin
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4

Zheng, Yunan. "Expanding the Scope of Multisite Noncanonical Amino Acid Mutagenesis." Thesis, Boston College, 2018. http://hdl.handle.net/2345/bc-ir:108150.

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Thesis advisor: Abhishek Chatterjee<br>Noncanonical amino acid (ncAA) mutagenesis provides powerful new ways to probe and manipulate protein function both in vitro and in living cells. Increasing the number of ncAAs that can be site-specifically encoded can greatly expand the scope of this promising technology. We aimed to address the challenges that limit the multisite ncAA incorporation technology in both Escherichia coli and mammalian cells. Our work has significantly expanded the scope of this technology through the development of mutually compatible suppression systems and the optimizatio
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5

Al, Saleem Evan. "Improving unnatural amino acid mutagensis efficiency and selectivity in mammalian cell." Thesis, KTH, Skolan för bioteknologi (BIO), 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-190765.

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Genetically encoded, site-specific incorporation of unnatural amino acids (UAA)into proteins through selective recoding of an amber stop codon provides apowerful route for expressing synthetic proteins in living cells. Recoding of theamber stop codon is achieved by introducing an amber suppressortRNA/synthetase pair orthogonal to the endogenous tRNA complement intocells. Methanosarcina is a methane producing archaea with the unusualcapability of suppressing the stop codon (specifically the amber codon). Bysuppressing the amber codon Methanosarcina facilitate the incorporation of thenon-canonic
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6

Nguyen, Duy Phuoc. "Unnatural amino acid incorporation via the orthogonal pyrrolysyl-tRNA synthetase/tRNACUA pair." Thesis, University of Cambridge, 2012. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.610160.

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7

Erickson, Sarah. "Using Unnatural Amino Acid Incorporation to Modify and Manipulate Adeno-Associated Virus:." Thesis, Boston College, 2020. http://hdl.handle.net/2345/bc-ir:108955.

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Thesis advisor: Eranthie Weerapana<br>Adeno-Associated Virus (AAV) has been developed into a powerful therapeutic tool - in the last ten years it has acted as a gene-delivery vehicle in several approved therapeutics and many more therapeutics on trial. Despite extensive research, gaps in our understanding of AAV’s infectious cycle still exist, and further development is needed for the creation of improved gene therapy vectors. Technology to incorporate Unnatural Amino Acids (UAAs) into the AAV capsid has recently been developed, and could aid in both furthering our understanding of AAV’s biolo
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8

Qi, Xin Dervan Peter B. "Unnatural amino acid incorporation to rewrite the genetic code and RNA-peptide interactions /." Diss., Pasadena, Calif. : California Institute of Technology, 2005. http://resolver.caltech.edu/CaltechETD:etd-05272005-133323.

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9

Liu, Zhihua. "THE DESIGN AND SYNTHESIS OF NOVEL UNNATURAL AMINO ACIDS AND THE DESIGN AND SYNTHESIS OF PEPTIDES & PEPTIDOMIMETICS CONTAINING UNNATURAL AMINO ACIDS FOR THE STUDY OF G-PROTEIN COUPLED RECEPTORS." Diss., The University of Arizona, 2010. http://hdl.handle.net/10150/204274.

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Nature has gifted peptides as important modulators in the human body, but these types of molecules often have not been favored when we were looking for therapeutic agents. The poor bioavailability, fast degradation and until recent high manufacturing costs of some bioactive peptides lowered their potential usage in the health industry. Under these circumstances, unnatural amino acids were developed as indispensible tools providing enormous support to peptide science. By incorporating proper unnatural amino acids into a peptide or protein, we now can significantly improve peptide's or protein's
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10

Lee, Meng Huee. "Studies on ketoacid-dependent dioxygenases involved in amino acid metabolism." Thesis, University of Oxford, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.362049.

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11

Mitchell, Amanda. "Development of a Novel Genetically Encoded FRET System Using the Unnatural Amino Acid Anap." Thesis, Boston College, 2016. http://hdl.handle.net/2345/bc-ir:107177.

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Thesis advisor: Abhishek Chatterjee<br>Förster Resonance Energy Transfer (FRET) offers a powerful approach to study biomolecular dynamics in vitro as well as in vivo. The ability to apply FRET imaging to proteins in living cells provides an excellent tool to monitor important dynamic events such as protein conformational changes, protein-protein interactions, and proteolysis reactions. However, selectively incorporating two distinct fluorophores into the target protein(s) that are capable of FRET interaction within the complex cellular milieu is challenging. Consequently, terminal fusion to ge
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12

Bhushan, Bhaskar. "Unnatural amino acids as metal-mediated probes of biological function." Thesis, University of Oxford, 2014. http://ora.ox.ac.uk/objects/uuid:4b1cbed6-1151-4b9f-ad97-aa5765cc9384.

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Conjugation reactions on proteins have been used to access various post-translational modifications, for targeted delivery of drugs, for microscopy, and in studying receptor-ligand interactions. However, the ability to modify native proteins is constrained by the reactive functionalities of naturally occurring amino acids. This has driven research into the incorporation of unnatural amino acids (UAAs) into proteins. Research in this area has been motivated both by the possibility of increasing the breadth of chemical techniques for protein modification by introducing novel 'bio-orthogonal' rea
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13

Duodu, Portia. "Site-specific photo-proteolysis of proteins and peptides by incorporation of unnatural amino acid : synthesis and characterization of photo-activatable α-amino acid". Strasbourg, 2010. http://www.theses.fr/2010STRA6228.

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Les structures protéiques impliquées dans les phénomènes physiologiques comme la coagulation sanguine, l’apoptose ou la signalisation membranaire, nécessitent souvent une activation préliminaire de leurs formes pro-protéines inactives. Le présent travail de thèse s’inscrit dans le domaine de la biochimie, et porte sur le développement d’une photo-protéase comme un outil moléculaire original et efficace pour l’étude dynamique de protéines par photorégulation de leur activité. Les photo-protéases adoptent un concept identique à celui des précurseurs photolabile de biomolécules; En effet, l’irrad
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14

Gartland, Martin John. "Site-directed mutagenesis of the aromatic amino acid aminotransferase of Escherichia coli." Thesis, University of Nottingham, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.293014.

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15

Paine, Lisa Jane. "Chemical modification, mutagenesis and characterisation of the glycerol dehydrogenase from Bacillus stearothermophilus." Thesis, University of Southampton, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.316404.

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16

Johnson, Alexander M. "Building Platforms to Genetically Encode New Chemistry." Thesis, Boston College, 2017. http://hdl.handle.net/2345/bc-ir:107292.

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Thesis advisor: Abhishek Chatterjee<br>Abstract Unnatural amino acid (UAA) incorporation is a powerful tool used by biochemists to discover the nature of protein structure and function. The evolution of orthogonal aminoacyl-tRNA synthetase (aaRS)/tRNA pairs enables site-specific incorporation of UAAs proteins inside of living cells. The goal of this study was to further expand the repertoire of genetically encoded unnatural amino acids in E. coli as well as eukaryotes. We first attempted to engineer an aaRS, previously evolved for p-borono-phenylalanine (pBoF), to specifically charge 3-acetyl-
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17

Harvey, David J. "Functionalisation of spider silk protein 4RepCT using un-natural amino acid mutagenesis and click chemistry." Thesis, University of Nottingham, 2017. http://eprints.nottingham.ac.uk/40142/.

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Interest in spider silk as a biomaterial has recently increased owing to its strength, biocompatibility, biodegradability, lack of immunogenicity and pyrogenicity. Unfortunately large scale production from spiders is highly impractical, as their cannibalistic nature prevents them from being farmed. As a consequence of this, scalable production of recombinant spider silk sequences in E.coli has emerged. There is a demand for functionalised silk materials tailored for specific novel applications. Previously this has been achieved through genetic fusion, which can prove difficult when using highl
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18

Plona, Kathleen Lynn. "ROLE OF A CONSERVED AMINO ACID MOTIF IN LOCALIZATION OF HUMAN CLIC5 TO MICROVILLI." Ohio University Art and Sciences Honors Theses / OhioLINK, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=ouashonors1340830402.

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19

Italia, James Sebastian. "Development and Applications of Universal Genetic Code Expansion Platforms:." Thesis, Boston College, 2019. http://hdl.handle.net/2345/bc-ir:108354.

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Thesis advisor: Abhishek Chatterjee<br>The emergence of genetic code expansion (GCE) technology, which enables sitespecific incorporation of unnatural amino acids (UAAs) into proteins, has facilitated powerful new ways to probe and engineer protein structure and function. Using engineered orthogonal tRNA/aminoacyl-tRNA synthetase (aaRS) pairs that suppress repurposed nonsense codons, a variety of structurally diverse UAAs have been incorporated into proteins in living cells. This technology offers tremendous potential for deciphering the complex biology of eukaryotes, but its scope in eukaryot
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20

Schumann, Silvia, Mineko Terao, Enrico Garattini, et al. "Site directed mutagenesis of amino acid residues at the active site of mouse aldehyde oxidase AOX1." Universität Potsdam, 2009. http://opus.kobv.de/ubp/volltexte/2010/4503/.

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Mouse aldehyde oxidase (mAOX1) forms a homodimer and belongs to the xanthine oxidase family of molybdoenzymes which are characterized by an essential equatorial sulfur ligand coordinated to the molybdenum atom. In general, mammalian AOs are characterized by broad substrate specificity and an yet obscure physiological function. To define the physiological substrates and the enzymatic characteristics of mAOX1, we established a system for the heterologous expression of the enzyme in Eschericia coli. The recombinant protein showed spectral features and a range of substrate specificity similar to t
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21

Smith, Addison Kyle. "Molecular Dynamic Simulation of Protein Devices and the Parameterization of Azides and Alkynes for Use in Unnatural Amino Acid Models." BYU ScholarsArchive, 2021. https://scholarsarchive.byu.edu/etd/8783.

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Proteins that have been modified by attaching them to a surface or to a polyethylene glycol (PEG) molecule can see many uses in therapeutics and diagnostics -- these unique proteins are called protein devices. Current techniques can perform these functionalizations at a specific residue on the protein, but what remains is identifying what happens to protein structure when mutated, and where to perform the attachment. Both of these issues can be examined using molecular dynamic (MD) simulations. Currently, simulations of the unnatural amino acid (uAA) mutations necessary for protein device func
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22

Tyagi, Rajiv. "Crystal structure determination and site-directed mutagenesis of active site residues in Escherichia coli ketol-acid reductoisomerase /." [St. Lucia, Qld.], 2005. http://www.library.uq.edu.au/pdfserve.php?image=thesisabs/absthe18926.pdf.

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23

Levinson, Samantha D. "Enhancing Platforms at the Interface of Viruses and Directed Evolution:." Thesis, Boston College, 2021. http://hdl.handle.net/2345/bc-ir:109192.

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Thesis advisor: Abhishek Chatterjee<br>Directed evolution is a powerful technique to expand chemical space in biological systems. In particular, this method has been used to develop cellular machinery to enable genetic code expansion (GCE), the incorporation of unnatural amino acids (UAAs) into proteins during the translation process. GCE relies on evolving an aminoacyl tRNA synthetase (aaRS) and tRNA pair from a different domain of life to incorporate a UAA into proteins in their new host, as these evolutionarily distant pairs are less likely to be cross-reactive with host pairs. The aaRS and
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24

Delcorde, Julie. "Investigating Host-Viral Interactions in Liver Lipid Homeostasis and HCV Pathology." Thesis, Université d'Ottawa / University of Ottawa, 2014. http://hdl.handle.net/10393/31184.

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Hepatitis C virus (HCV) infects an estimated 170 million people worldwide and is a major cause of chronic hepatitis and hepatocellular carcinoma. As there are limited treatment options, the elucidation of novel host-viral interactions during HCV pathogenesis will be critical for the development of new therapeutics. My thesis work has identified cell death-inducing DFF45-like effector B (CIDEB) as a host factor that is disregulated during HCV infection, and has delineated the relevance of CIDEB’s dual roles in apoptosis and lipid metabolism in the context of the HCV lifecycle. Moreover, additi
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25

Cristea, Mirela. "Expression of Manganese Lipoxygenase and Site-Directed Mutagenesis of Catalytically Important Amino Acids : Studies on Fatty Acid Dioxygenases." Doctoral thesis, Uppsala : Acta Universitatis Upsaliensis : Universitetsbiblioteket [distributör], 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-6625.

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26

Evans, Steven John. "Structure, function and mechanism of action of bovine pancreatic deoxyribonuclease I : role of amino acid residues involved in phosphate contacts." Thesis, University of Newcastle Upon Tyne, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.321857.

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27

Banerjee, Koushik. "Investigations in amine chemistry: Mn-Mediated radical addition approach toward gamma amino esters and synthetic studies of the tubulysins." Diss., University of Iowa, 2011. https://ir.uiowa.edu/etd/1121.

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Mn-Mediated radical addition has been developed within the Friestad laboratory as a versatile method toward addition to C=N bonds. N-Acylhydrazones generated by condensation between an aldehyde and an N-acylamine serves as the substrate toward radical addition. A bulky directed group attached with the N-acyl moiety and restricted rotation around N-N bond due to a three point chelation with a Lewis acid differentiates the faces of the C=N bond of the N-acylhydrazones. Radical generation initiated by photolysis of Mn2(CO)10 causing homolysis of C-X bond in alkyl halide serves as the radical dono
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28

Broadbent, Andrew. "Aminoacyl-tRNA Synthetase Production for Unnatural Amino Acid Incorporation and Preservation of Linear Expression Templates in Cell-Free Protein Synthesis Reactions." BYU ScholarsArchive, 2016. https://scholarsarchive.byu.edu/etd/5703.

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Proteins—polymers of amino acids—are a major class of biomolecules whose myriad functions facilitate many crucial biological processes. Accordingly, human control over these biological processes depends upon the ability to study, produce, and modify proteins. One innovative tool for accomplishing these aims is cell-free protein synthesis (CFPS). This technique, rather than using living cells to make protein, simply extracts the cells' natural protein-making machinery and then uses it to produce protein in vitro. Because living cells are no longer involved, scientists can freely adapt the prote
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Schinn, Song Min. "Cell-Free Synthesis of Proteins with Unnatural Amino Acids: Exploring Fitness Landscapes, Engineering Membrane Proteins and Expanding the Genetic Code." BYU ScholarsArchive, 2017. https://scholarsarchive.byu.edu/etd/6496.

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Unnatural amino acids (uAA) expand the structural and functional possibilities of proteins. Numerous previous studies have demonstrated uAA as a powerful tool for protein engineering, but challenges also remain. Three notable such challenges include: (1) the fitness of uAA-incorporated proteins are difficult to predict and time-consuming to screen with conventional methods, (2) uAA incorporation in difficult-to-express proteins (e.g. membrane proteins such as G-protein coupled receptors) remain challenging, and (3) the incorporation of multiple types of uAA are still limited. In response, we p
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Roberts, Sarah Elizabeth. "Synthesis of 2,4,5-Triaminocyclohexane Carboxylic Acid as a Novel 2-Deoxystreptamine Mimetic." Diss., CLICK HERE for online access, 2009. http://contentdm.lib.byu.edu/ETD/image/etd2890.pdf.

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31

Adepoju, Olusegun A., Devaiah K. Shiva, and Cecelia A. McIntosh. "Using Site-Directed Mutagenesis to Determine Impact of Amino Acid Substitution on Substrate and Regiospecificity of Grapefruit Flavonol 3-O-Glucosyltransferase." Digital Commons @ East Tennessee State University, 2014. https://dc.etsu.edu/etsu-works/346.

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Flavonoids are secondary metabolites that are important in plant defense, protection and human health. Most naturally-occurring flavonoids are found in glucosylated form. Glucosyltransferases (GTs) are enzymes that catalyze the transfer of glucose from a high energy sugar donor to an acceptor molecule. A flavonol-specific 3-O-GT enzyme has been identified and cloned from leaf tissues of grapefruit. The enzyme shows rigid substrate specificity and regiospecificity. F3-O-GTs from grape (Vitis vinifera) and grapefruit (Citrus paradisi) were modeled against F7-O-GTs from Crocus sativus and Scrutel
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32

Ghaddar, Kassem. "Structural analysis of yeast amino acid transporters: substrate binding and substrate-induced endocytosis." Doctoral thesis, Universite Libre de Bruxelles, 2014. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/209318.

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Plasma membrane transport proteins play a crucial role in all cells by conferring to the cell surface a selective permeability to a wide range of ions and small molecules. The activity of these transporters is often regulated by controlling their amount at the plasma membrane, via intracellular trafficking. The recent boom in the numbers of crystallized transporters shows that many of them that belong to different functional families with little sequence similarity adopt the same structural fold implying a conserved transport mechanism. These proteins belong to the APC (Amino acid-Polyamine-or
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Adepoju, Olusegun Adeboye. "Using Site-Directed Mutagenesis to Determine Impact of Amino Acid Substitution on Substrate and Regiospecificity of Grapefruit Flavonol Specific 3-O-Glucosyltransferase." Digital Commons @ East Tennessee State University, 2014. https://dc.etsu.edu/etd/2404.

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Flavonoids are secondary metabolites that are important in plant defense, protection, and human health. Most naturally-occurring flavonoids are found in glucosylated forms. Glucosyltransferases catalyze the transfer of glucose from high-energy sugar donors to an acceptor molecule. The grapefruit flavonol-specific 3-O-glucosyltransferase (F3-O-GT) is highly substrate and regio-specific. The goal of this research is to unravel the amino acid residues responsible for the grapefruit enzyme’s rigid specificity, while attempting to alter the regiospecific glucosylation pattern through site-directed
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Davenport, Eric Parker. "Fluorescent Probes to Investigate Homologous Recombination Dynamics." DigitalCommons@USU, 2016. https://digitalcommons.usu.edu/etd/5007.

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There are multiple mechanisms by which DNA can become damaged. Such damage must be repaired for the cell to avoid ill-health consequences. Homologous recombination (HR) is a means of repairing one specific type of damage, a double-strand break (DSB). This complex pathway includes the Rad51-DNA nucleoprotein filament as its primary machinery. Current methodology for studying HR proteins includes the use of fluorescently labeled DNA to probe for HR dynamics. This technique limits the number of proteins that can be involved in experimentation, and often only works as an end reporter. The work her
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35

Mayo, Daniel J. "Synthetic methodologies for labeling membrane proteins and studies utilizing electron paramagnetic resonance in biologically relevant lipid architectures." Miami University / OhioLINK, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=miami1343434201.

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Roscoe, Benjamin P. "Analyses of All Possible Point Mutations within a Protein Reveals Relationships between Function and Experimental Fitness: A Dissertation." eScholarship@UMMS, 2003. http://escholarship.umassmed.edu/gsbs_diss/716.

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The primary amino acid sequence of a protein governs its specific cellular functions. Since the cracking of the genetic code in the late 1950’s, it has been possible to predict the amino acid sequence of a given protein from the DNA sequence of a gene. Nevertheless, the ability to predict a protein’s function from its primary sequence remains a great challenge in biology. In order to address this problem, we combined recent advances in next generation sequencing technologies with systematic mutagenesis strategies to assess the function of thousands of protein variants in a single experiment. U
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Roscoe, Benjamin P. "Analyses of All Possible Point Mutations within a Protein Reveals Relationships between Function and Experimental Fitness: A Dissertation." eScholarship@UMMS, 2014. https://escholarship.umassmed.edu/gsbs_diss/716.

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The primary amino acid sequence of a protein governs its specific cellular functions. Since the cracking of the genetic code in the late 1950’s, it has been possible to predict the amino acid sequence of a given protein from the DNA sequence of a gene. Nevertheless, the ability to predict a protein’s function from its primary sequence remains a great challenge in biology. In order to address this problem, we combined recent advances in next generation sequencing technologies with systematic mutagenesis strategies to assess the function of thousands of protein variants in a single experiment. U
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Kirov, Miroslav [Verfasser], Lutz [Akademischer Betreuer] Schmitt, and Ulrich [Akademischer Betreuer] Schulte. "The incorporation of an unnatural amino acid to study the nucleotide binding domain of the ABC transporter HlyB from Escherichia coli / Miroslav Kirov. Gutachter: Ulrich Schulte. Betreuer: Lutz Schmitt." Düsseldorf : Universitäts- und Landesbibliothek der Heinrich-Heine-Universität Düsseldorf, 2014. http://d-nb.info/1051076803/34.

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Wilding, Kristen Michelle. "Engineering Cell-Free Biosystems for On-Site Production and Rapid Design of Next-Generation Therapeutics." BYU ScholarsArchive, 2018. https://scholarsarchive.byu.edu/etd/7713.

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While protein therapeutics are indispensable in the treatment of a variety of diseases, including cancer, rheumatoid arthritis, and diabetes, key limitations including short half-lives, high immunogenicity, protein instability, and centralized production complicate long-term use and on-demand production. Site-specific polymer conjugation provides a method for mitigating these challenges while minimizing negative impacts on protein activity. However, the location-dependent effects of polymer conjugation are not well understood. Cell-free protein synthesis provides direct access to the synthesis
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Ring, Christine. "Optimization of in vitro transcription/translation conditions for in vitro compartmentalization studies and synthesis of 4-fluorohistidine." VCU Scholars Compass, 2017. http://scholarscompass.vcu.edu/etd/4807.

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Genetic code expansion allows the incorporation of non-canonical amino acids with a variety of new functional groups: fluorescent amino acids,1-3 azides,4-6 alkynes,5-10 and photocrosslinkers.4,11,12 This incorporation requires the evolution of new tRNA/aminoacyl tRNA sythetase pairs. Traditionally screenings of novel tRNA/aminoacyl tRNA synthetase pairs have been done in vivo. While these in vivo screenings have proven robust, they are limited in multiple ways: non-canonical amino acids (ncAAs) must be nontoxic and bioavailable. Furthermore, library size is limited by transformation eff
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Saliba, Elie. "A NOVEL TORC1 ACTIVATION PATHWAY STIMULATED BY THE PLASMA MEMBRANE H+-ATPASE UNRAVELED FROM THE STUDY OF SUBSTRATE-INDUCED ENDOCYTOSIS OF AMINO ACID TRANSPORTERS IN YEAST SACCHAROMYCES CEREVISIAE." Doctoral thesis, Universite Libre de Bruxelles, 2017. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/262158.

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Chez les eucaryotes, le complexe kinase TORC1 (Target Of Rapamycin Complex 1) joue un rôle central dans le contrôle de la croissance cellulaire. Il intègre de nombreux signaux et agit en modulant l’état de phosphorylation de différents effecteurs, principalement des protéines impliquées dans des processus anaboliques ou cataboliques. Parmi ces signaux, on distingue notamment les acides aminés. Ces derniers agissent sur TORC1 via l’action de protéines de la famille des GTPases Rag, elles-mêmes régulées par des facteurs GEF et GAP. Des études récentes sur des cellules mammifères ont mis en évide
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Knappenberger, Andrew John. "MOLECULAR DRIVERS OF SPECIFICITY IN HUMAN RIBONUCLEOTIDE REDUCTASE." Case Western Reserve University School of Graduate Studies / OhioLINK, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=case1490722285574706.

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43

Legault, Marc. "From Probes to Cell Surface Labelling: Towards the Development of New Chemical Biology Compounds and Methods." Thèse, Université d'Ottawa / University of Ottawa, 2011. http://hdl.handle.net/10393/20084.

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Chemical biology encompasses the study and manipulation of biological system using chemistry, often by virtue of small molecules or unnatural amino acids. Much insight has been gained into the mechanisms of biological processes with regards to protein structure and function, metabolic processes and changes between healthy and diseased states. As an ever expanding field, developing new tools to interact with and impact biological systems is an extremely valuable goal. Herein, work is described towards the synthesis of a small library of heterocyclic-containing small molecules and the mechanisti
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44

Hegazy, Usama M. "Structure-Function Relationships of Pi Class Glutathione Transferase Studied by Protein Engineering." Doctoral thesis, Uppsala : Acta Universitatis Upsaliensis, 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-7146.

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45

Nick, Thomas Udo. "Hydrogen Bonds and Electrostatic Environment of Radical Intermediates in Ribonucleotide Reductase Ia." Doctoral thesis, Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2015. http://hdl.handle.net/11858/00-1735-0000-0028-877F-7.

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46

Modén, Olof. "Mutational Analysis and Redesign of Alpha-class Glutathione Transferases for Enhanced Azathioprine Activity." Doctoral thesis, Uppsala universitet, Biokemi, 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-167332.

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Glutathione transferase (GST) A2-2 is the human enzyme most efficient in catalyzing azathioprine activation. Structure-function relationships were sought explaining the higher catalytic efficiency compared to other alpha class GSTs. By screening a DNA shuffling library, five recombined segments were identified that were conserved among the most active mutants. Mutational analysis confirmed the importance of these short segments as their insertion into low-active GSTs introduced higher azathioprine activity. Besides, H-site mutagenesis led to decreased azathioprine activity when the targeted po
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47

Zhang, Wei. "Directed Evolution of Glutathione Transferases with Altered Substrate Selectivity Profiles : A Laboratory Evolution Study Shedding Light on the Multidimensional Nature of Epistasis." Doctoral thesis, Uppsala universitet, Biokemi, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-158400.

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Directed evolution is generally regarded as a useful approach in protein engineering. By subjecting members of a mutant library to the power of Darwinian evolution, desired protein properties are obtained. Numerous reports have appeared in the literature showing the success of tailoring proteins for various applications by this method. Is it a one-way track that protein practitioners can only learn from nature to enable more efficient protein engineering? A structure-and-mechanism-based approach, supplemented with the use of reduced amino acid alphabets, was proposed as a general means for sem
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48

Keränen, Henrik. "Advances in Ligand Binding Predictions using Molecular Dynamics Simulations." Doctoral thesis, Uppsala universitet, Beräknings- och systembiologi, 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-230777.

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Biochemical processes all involve associations and dissociations of chemical entities. Understanding these is of substantial importance for many modern pharmaceutical applications. In this thesis, longstanding problems with regard to ligand binding are treated with computational methods, applied to proteins of key pharmaceutical importance. Homology modeling, docking, molecular dynamics simulations and free-energy calculations are used here for quantitative characterization of ligand binding to proteins. By combining computational tools, valuable contributions have been made for pharmaceutical
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49

Makki, Arwa. "From Unnatural Amino Acid Incorporation to Artificial Metalloenzymes." Diss., 2016. http://hdl.handle.net/10754/621994.

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Studies and development of artificial metalloenzymes have developed into vibrant areas of research. It is expected that artificial metalloenzymes will be able to combine the best of enzymatic and homogenous catalysis, that is, a broad catalytic scope, high selectivity and activity under mild, aqueous conditions. Artificial metalloenzyme consist of a host protein and a newly introduced artificial metal center. The host protein merely functions as ligand controlling selectivity and augmenting reactivity, while the metal center determines the reactivity. Potential applications range from catal
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50

Singh, Amrita active 2012. "Protein evolution in the presence of an unnatural amino acid." Thesis, 2012. http://hdl.handle.net/2152/23413.

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The field of protein engineering has been greatly augmented by the expansion of the genetic code using unnatural amino acids as well as the development of cell-free synthesis systems with high protein yield. Cell-free synthesis systems have improved considerably since they were first described almost 40 years ago. Residue specific incorporation of non-canonical amino acids into proteins is usually performed in vivo using amino acid auxotrophic strains and replacing the natural amino acid with an unnatural amino acid analog. Herein, we present an amino acid depleted cell-free protein synthesis
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