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Dissertations / Theses on the topic 'Vibrio. luxR'

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Published: 4 June 2021

Last updated: 1 February 2022

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1

Faini, Marie Annette. "Transcriptional Control during Quorum Sensing by LuxR and LuxR Homologues." Thesis, Virginia Tech, 2003. http://hdl.handle.net/10919/31994.

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Quorum sensing is a mechanism used by many proteobacteria to regulate expression of target genes in a population-dependent manner. The quorum sensing system of Vibrio fischeri activates the luminescence (lux) operon when the autoinducer signaling molecule (N-3-oxohexanoyl homoserine lactone) is recognized and bound by the activator protein LuxR. LuxR subsequently binds to the lux box centered at Ã 42.5 bp upstream of the transcription initiation site and activates transcription from the lux operon promoter, resulting in the emission of light at high cell densities. LuxR consists of 250 amino

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2

Qin, Nan. "Analysis of the Regulons Controlled by Transcriptional Regulators LuxR and LitR in Vibrio fischeri." Diss., Virginia Tech, 2008. http://hdl.handle.net/10919/28433.

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Quorum sensing is a bacterial signaling system that controls gene expression in a population density-dependent manner. In Gram-negative proteobacteria, the cell density control of luminescence was first observed in the symbiotic marine bacterium Vibrio fischeri and this system is one of the best studied quorum sensing systems. Two-dimensional sodium dodecyl sulfate-polyacrylamide (2D-SDS) gel electrophoresis analysis previously identified several non-Lux proteins in V. fischeri MJ-100 whose expression was dependent on LuxR and 3-oxo-hexanoyl-L-homoserine lactone (3-oxo-C6-HSL). A lacZ reporter

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3

Callahan, Sean M. (Sean Michael) 1966. "The quorum-sensing regulation of Vibrio fischeri : novel components of the autoinduce/LuxR regulatory circuit." Thesis, Massachusetts Institute of Technology, 1999. http://hdl.handle.net/1721.1/85290.

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4

Williams, Joshua W. "Multi-tiered Regulation of luxR Provides Precise Timing and Maintenance of the Quorum Sensing Response of Vibrio fischeri." Diss., Virginia Tech, 2009. http://hdl.handle.net/10919/38580.

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The quorum-sensing response of Vibrio fischeri involves a complex network of genes (encoding regulatory proteins as well as sRNAs), that govern host-association and production of bioluminescence. A key regulator of this system is LuxR, which is the transcriptional activator of the lux operon as well as several other genes in. LuxR also autoregulates its own transcription, which we have shown causes bistability and hysteresis in the quorum-sensing response. This behavior allows the system to maintain a stable and robust response in the face of environmental fluctuation or decreases in extern

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5

Chatterjee, Jaidip. "The luminescence induction point of Vibrio harveyi is an integration of multiple regulatory controls : LuxR, MetR, and CRP." Thesis, McGill University, 2000. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=36889.

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Luminescence is a secondary metabolic process that can be detected and assayed in a non-disruptive, in vivo, and real-time manner. As such the Vibrio harveyi luminescence (lux) system constitutes a model system that can be used to delineate the relatively complex transcriptional control mechanisms that are inherent to secondary metabolic processes. Molecular insights into the control of luminescence induction in V. harveyi is limited to the two-component quorum-sensing system and LuxR. While the quorum-sensing system is thought to facilitate luminescence induction through a mechanism of derepr

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6

Trott, Amy Elizabeth. "Amino Acid Residues in LuxR Critical for its Mechanism of Transcriptional Activation during Quorum Sensing." Thesis, Virginia Tech, 2000. http://hdl.handle.net/10919/34070.

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<I>Vibrio fischeri</I>, a symbiotic bioluminescent bacterium, serves as one of the best understood model systems for a mechanism of cell-density dependent bacterial gene regulation known as quorum sensing. During quorum sensing in <I>V. fischeri</I>, an acylated homoserine chemical signal (autoinducer) is synthesized by the bacteria and used to sense their own species in a given environment. As the autoinducer levels rise, complexes form between the autoinducer and the N-terminal domain of a regulatory protein, LuxR. In response to autoinducer binding, LuxR is believed to undergo a conformatio

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7

McDougald, S. Diane School of Microbiology &amp Immunology UNSW. "Regulation of starvation and nonculturability in the marine pathogen, Vibrio vulnificus." Awarded by:University of New South Wales. School of Microbiology and Immunology, 2000. http://handle.unsw.edu.au/1959.4/19118.

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Vibrio vulnificus is a model environmental organism exhibiting a classical starvation response during nutrient limitation as well as a non-culturable state when exposed to low temperatures. In addition to these classic global responses, this organism is an opportunistic pathogen that exhibits numerous virulence factors. This organism was chosen as the model organism for the identification of regulators of the viable but nonculturable response (VBNC) and the starvation-induced maintenance of culturability (SIMC) that occurs when cells are starved prior to low temperature incubation. In order

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8

Johnson, Deborah Cumaraswamy. "Role of region 4 of the sigma 70 subunit of RNA polymerase in transcriptional activation of the lux operon during quorum sensing." Thesis, Virginia Tech, 2002. http://hdl.handle.net/10919/31680.

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The mechanism of gene regulation used by Gram-negative bacteria during quorum sensing is well understood in the bioluminescent marine bacterium Vibrio fischeri. The cell-density dependent activation of the luminescence (lux) genes of V. fischeri relies on the formation of a complex between the autoinducer molecule, N-(3-oxohexanoyl) homoserine lactone, and the autoinducer-dependent transcriptional activator LuxR. LuxR, a 250 amino acid polypeptide, binds to a site known as the lux box centered at position -42.5 relative to the luxI transcriptional start site. During transcriptional activati

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9

Finney, Angela H. "Role of the C-terminal domain of the a subunit of RNA polymerase in transcriptional activation of the lux operon during quorum sensing." Thesis, Virginia Tech, 2000. http://hdl.handle.net/10919/36285.

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Quorum sensing in Gram-negative bacteria is best understood in the bioluminescent marine microorganism, <i>Vibrio fischeri</i>. In <i>V. fischeri</i>, the luminescence or <i>lux</i> genes are regulated in a cell density-dependent manner by the activator LuxR in the presence of an acylated homoserine lactone autoinducer molecule (3-oxo-hexanoyl homoserine lactone). LuxR, which binds to the <i>lux</i> operon promoter at position -42.5, is thought to function as an ambidextrous activator making multiple contacts with RNA polymerase (RNAP). The specific role of the <font face = "symbol">a</fon

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10

Odenbach, Tina. "Charakterisierung der Hybridsensorkinase LuxN und des Antwortregulators LuxO des Quorum sensing-Systems in Vibrio harveyi." Diss., lmu, 2009. http://nbn-resolving.de/urn:nbn:de:bvb:19-100461.

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11

Lin, Yi Hsing 1969. "Purification, cloning, and functional studies of a new transcriptional factor luxt from Vibrio harveyi." Thesis, McGill University, 2000. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=37765.

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A luxO DNA binding protein (LuxT) was purified to homogeneity from V. harveyi after five major chromatography steps including a highly effective DNA affinity chromatography step and reverse phase HPLC. The sequences of three tryptic peptides obtained on digestion of the purified protein did not match any sequences in the protein data bank indicating that LuxT is a new V. harveyi protein. Inverse PCR was conducted sequentially to obtain the complete gene (luxT) coding for a protein of 153 amino acids which shares homology with the AcrR/TetR family of transcriptional regulators. Gene disruption

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12

Srinivasa, Sandeep S. "Testing the Hypothesis of Quorum Sensing in Vibrio fischeri : Luminescence, Motility, and Biofilm." Thesis, 2017. http://hdl.handle.net/2005/3270.

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The individual behaviour of prokaryotic organisms such as bacteria often gives rise to complexity that is commonly associated with multicellular behaviour. The transition from unicellular to multicellular behaviour occurs in response to chemical signals, called autoinducers, which bacteria generate and receive internally within a given population. These autoinducers control the gene expression necessary for the emergence of group-behaviour-phenotype. This phenomenon is called quorum sensing (QS). An example of the quorum sensing control of gene regulation has been the luminescence (lux) operon

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13

Wu, Sin-Syuan, and 吳昕玹. "Studies on the Effect of Capsular Polysaccharides on Formation of Biofilm and Expression of Quorum-sensing Regulators LuxR/SmcR in Vibrio vulnificus." Thesis, 2013. http://ndltd.ncl.edu.tw/handle/21810891370752940076.

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碩士<br>輔英科技大學<br>醫學檢驗生物技術系碩士班<br>101<br>Vibrio vulnificus is a halophilic gram-negative bacterial species. In nature, V. vulnificus is present in marine environment and causes opportunistic infections in humans including septicemia and wound infection. The capsular polysaccharide is one of potential virulence factors and associated with formation of biofilm. To understand the effect of capsular polysaccharide on the formation of biofilm, this study uses the acapsular strain JF046 (ΔORFVV0364) with a mutation in capsular polysaccharide surface factor. The characteristics of bacterial growth and

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14

Odenbach, Tina [Verfasser]. "Charakterisierung der Hybridsensorkinase LuxN und des Antwortregulators LuxO des Quorum-sensing-Systems in Vibrio harveyi / vorgelegt von Tina Odenbach." 2008. http://d-nb.info/994429231/34.

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15

Huang, Yan-Wei, and 黃彥瑋. "Cloning and Application of lux Genes from Vibrio orientalis." Thesis, 2011. http://ndltd.ncl.edu.tw/handle/89604666031997168514.

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碩士<br>國立屏東科技大學<br>生物科技研究所<br>100<br>Communication among bacteria with quorum-sensing system is accomplished through the exchange of signaling molecules called autoinducers. When the density of autoinducers accumulated to a certain concentration, bacteria responded by specialized gene expression such as genes involved in biofilm formation, bioluminescence, virulence and sporulation. Currently it still remains unclear about the light-emitting and regulatory mechanism of the marine luminous bacteria V. orientalis. It is speculated that the expression of bioluminescence genes in V. orientalis is a

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16

Ariestantia, Tiara Eka, and 堤心亞. "Study on Regulation of lux Genes by HapR from Vibrio orientalis." Thesis, 2017. http://ndltd.ncl.edu.tw/handle/5872mk.

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碩士<br>國立屏東科技大學<br>生物科技系所<br>106<br>The process of communication from one cell to another in bacteria called Quorum Sensing (QS). This process can regulate the genes that must be perform in a group of bacteria. In V. orientalis, the luminous bacteria from Yellow coast of China there are six genes that linked with QS. That genes were: luxP (VIA_001151), luxQ (VIA_001152), luxS (VIA_003963), luxO (VIA_003573) and (VIA_003054), luxU (VIA_003574) and hapR (VIA_000273). This research focused on hapR gene since it was LuxR homologues and previous report showed that LuxR is an activator of the lux op

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17

Lin, Chen-Hsing, and 林貞杏. "Role of LuxO, a quorum-sensing regulator, in Vibrio vulnificus virulence in mice." Thesis, 2004. http://ndltd.ncl.edu.tw/handle/95848071150459619155.

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碩士<br>國立成功大學<br>微生物及免疫學研究所<br>92<br>Vibrio vulnificus, a halophilic gram-negative marine bacterium, is an opportunistic pathogen causing septicemia and wound infection in humans. A few virulence factors, such as the capsule, a few extracellular products, and iron-acquisition ability, have been proposed; however, the pathogenesis mechanism of this organism remains unclear. It has been shown previously that the quorum-sensing system can regulate the virulence of pathogens, such as V. cholerae, Pseudomonas aeruginosa, and Staphylococcus aureus. In the whole genome sequence of V. vulnificus comple

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18

Chen, Li-Ming, and 陳立明. "Functional analysis of the sub-regulation in the lux regulon of Vibrio fishcheri." Thesis, 1997. http://ndltd.ncl.edu.tw/handle/45391069413987290789.

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碩士<br>國立中興大學<br>分子生物研究所<br>85<br>V. fishcheri lux regulon luxR gene 下游 3,565 bp 之 DNA 經定序分析，得知此 DNA 片段，具有 RNA-like gene cluster、ufo1 gene、 origin-like sequence 與 cnf1 gene，與 lux regulon 之排列序列為 ----cnf1-ori-like sequence-ufo1-RNA-like gene cluster-R&R'-luxR- R&R-luxI-luxC-luxD-luxA-luxB-luxE----。其中 cnf1 為主導 cytotoxic necrotizing factor 1 基因；ufo1 為一未鑑定之基因；而 RNA-like gene cluster 包括一 rRNA-like gene 與數個 tRNA-like genes；另存在一 origin-like sequence。此等基因顯然受 lux regulon 主調節區域 R&R (reg

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19

Chen, Yun-Ju, and 陳韻如. "Influence of quorum sensing component LuxS on physiological changes and gene expression in Vibrio parahaemolyticus." Thesis, 2011. http://ndltd.ncl.edu.tw/handle/12991232463169780756.

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碩士<br>慈濟大學<br>微生物學免疫學暨生物化學研究所<br>99<br>Quorum sensing system, a two-component system in bacteria, is a cell-cell communication process. Bacteria use the production and detection of the extracellular chemical signals called autoinducers (AI) to monitor cell density and coordinate gene expression. The LuxS enzyme (S-ribosylhomocysteinlyase), which is conserved in a wide variety of bacteria, is responsible for the production of autoinduer-2 (AI-2). It has been reported that LuxS/AI-2 system has multiple roles in interfering with physiological and virulence functions in many bacteria. To gain insi

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20

Timmen, Melanie. "in vitro-Rekonstruktion der Quorum sensing-Signaltransduktionskaskade zur Charakterisierung der Hybridsensorkinase LuxN aus Vibrio harveyi." Doctoral thesis, 2005. https://repositorium.ub.uni-osnabrueck.de/handle/urn:nbn:de:gbv:700-2005061330.

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Mittels Quorum sensing können Bakterienzellen die Expression von Genen Zelldichte-abhängig steuern. Dies spielt eine besondere Rolle bei der Expression von Virulenzfaktoren oder Antibiotikaproduktion, der Biofilmentwicklung oder Phänomenen wie genetischer Kompetenz, Sporulation oder Biolumineszenz. Vibrio harveyi, ein Gram-negativer, mariner, frei lebender Organismus, reguliert die Expression von Biolumineszenz-Genen Zelldichte-abhängig nach dem Prinzip des Quorum sensing und sollte im Rahmen dieser Arbeit als Modell für die Mechanismen der Signaltransduktion untersucht werden. Dazu wurden die

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21

Timmen, Melanie [Verfasser]. "In-vitro-Rekonstruktion der Quorum-sensing-Signaltransduktionskaskade zur Charakterisierung der Hybridsensorkinase LuxN aus Vibrio harveyi / von Melanie Timmen." 2005. http://d-nb.info/975746219/34.

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22

Man-Ling, Tu, and 塗曼鈴. "Gene Expression and Regulation of Bioluminescence:Functional Analysis of the Regulatory Region of the lux Regulon from Vibrio fischeri." Thesis, 1993. http://ndltd.ncl.edu.tw/handle/33811004831398274375.

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碩士<br>國立中興大學<br>分子生物研究所<br>81<br>We use the gene order rearrangement to analyze the regulatory region (R&R) of the lux regulon from Vibrio fischeri reveals that the expression of the L-promoter is much stronger than that of the R-promoter. The results of glucose repression experiment illustrate that the expression of the L-operon is enhanced by cAMP-CRP. In trans complementation tests elucidat that the LuxR- AI complex could enhance the expression of the two promoters by binding on the mo

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23

Chen, Hui-Yi, and 陳惠. "Gene Expression and Regulation of Bioluminescence: Functional Analysis of the Regulatory Gene of the lux Regulon form Vibrio fischeri." Thesis, 1993. http://ndltd.ncl.edu.tw/handle/54920807387796787431.

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碩士<br>國立中興大學<br>分子生物研究所<br>81<br>For the purpose of simplicity, only the regulatory genes, luxR and luxI, were used for studying in this work. A series of gene order rearrangement constructions and in trans comple- mentary tests were designed to investigate the requirement of the sequences of the luxR and luxI genes, and functional analy- sis of the LuxR protein and autoinducer (AI). The luciferase genes, luxA-luxB, from V. harveyi were used as reporter gene to monitor the expression of the

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