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Статті в журналах з теми "Psychosocial Enzyme-linked immunosorbent assay (ELISA)":
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Monteleone, P., P. Scognamiglio, B. Canestrelli, I. Serino, A. M. Monteleone та M. Maj. "Asymmetry of salivary cortisol and α-amylase responses to psychosocial stress in anorexia nervosa but not in bulimia nervosa". Psychological Medicine 41, № 9 (2 лютого 2011): 1963–69. http://dx.doi.org/10.1017/s0033291711000092.
BackgroundThe stress response involves the activation of the hypothalamic–pituitary–adrenal (HPA) axis and the sympathetic nervous system (SNS). As a role for stress in determining of the onset and the natural course of eating disorders (EDs) has been proposed, the study of the psychobiology of the stress response in patients with anorexia nervosa (AN) and bulimia nervosa (BN) should be helpful in understanding the pathophysiology of these disorders. The two neurobiological components of the stress response can be easily explored in humans by the measurement of salivary cortisol and α-amylase response to a stressor. Therefore, we assessed salivary cortisol and α-amylase responses to the Trier Social Stress Test (TSST) in symptomatic patients with AN and BN compared to healthy controls.MethodSeven AN women, eight BN women and eight age-matched healthy females underwent the TSST between 1530 and 1700 h. Salivary cortisol and α-amylase levels were measured by an enzyme-linked immunosorbent assay (ELISA).ResultsCompared to healthy women, AN patients showed a normal cortisol response to the TSST, although this occurred at significantly increased hormone levels, and an almost complete absence of response of α-amylase. BN women, however, exhibited enhanced pre-stress levels of salivary α-amylase but a normal response of the enzyme and cortisol to the TSST.ConclusionsThese findings demonstrate, for the first time, the occurrence of an asymmetry between the HPA axis and SNS components of the stress response in the acute phase of AN but not in BN. The pathophysiological significance of this asymmetry remains to be determined.
Abstract This brief note addresses the historical background of the invention of the enzyme immunoassay (EIA) and enzyme-linked immunosorbent assay (ELISA). These assays were developed independently and simultaneously by the research group of Peter Perlmann and Eva Engvall at Stockholm University in Sweden and by the research group of Anton Schuurs and Bauke van Weemen in The Netherlands. Today, fully automated instruments in medical laboratories around the world use the immunoassay principle with an enzyme as the reporter label for routine measurements of innumerable analytes in patient samples. The impact of EIA/ELISA is reflected in the overwhelmingly large number of times it has appeared as a keyword in the literature since the 1970s. Clinicians and their patients, medical laboratories, in vitro diagnostics manufacturers, and worldwide healthcare systems owe much to these four inventors.
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Shah, Karishma, and Panagiotis Maghsoudlou. "Enzyme-linked immunosorbent assay (ELISA): the basics." British Journal of Hospital Medicine 77, no. 7 (July 2, 2016): C98—C101. http://dx.doi.org/10.12968/hmed.2016.77.7.c98.
Kohl, Thomas O., and Carl A. Ascoli. "Direct Competitive Enzyme-Linked Immunosorbent Assay (ELISA)." Cold Spring Harbor Protocols 2017, no. 7 (July 2017): pdb.prot093740. http://dx.doi.org/10.1101/pdb.prot093740.
Kohl, Thomas O., and Carl A. Ascoli. "Indirect Competitive Enzyme-Linked Immunosorbent Assay (ELISA)." Cold Spring Harbor Protocols 2017, no. 7 (July 2017): pdb.prot093757. http://dx.doi.org/10.1101/pdb.prot093757.
A B S T R A C TELISA (Enzyme-linked immunosorbent assay) is a technique used to assessthe quantification of peptide, protein, antibody and hormone levels, basedon the principle of antigen-antibody binding. In the ELISA technique, antigenimmobilization will be carried out on a solid surface, then bound withantibodies to form an antigen-antibody bond complex, where the antigen-antibody complex is bound to the enzyme. The detection signal in the formof a color change will be formed due to the reaction between the enzyme andthe substrate.
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Cleary, J. D., S. W. Chapman, J. Deng, and C. J. Lobb. "Amphotericin B enzyme-linked immunosorbent assay." Antimicrobial Agents and Chemotherapy 40, no. 3 (March 1996): 637–41. http://dx.doi.org/10.1128/aac.40.3.637.
Our purpose was to develop and characterize an enzyme-linked immunosorbent assay (ELISA) which could measure the concentration of amphotericin B in serum. Amphotericin B was assayed by competition ELISA. Multiwell ELISA plates coated with amphotericin B (1.0 micrograms/ml) conjugated to bovine serum albumin were used to test replicates of serum samples spiked with amphotericin B. Purified rabbit polyclonal antibody against amphotericin B (1.4 micrograms/ml) was added subsequent to the instillation of samples spiked with unknown amounts of amphotericin B. Experiments were performed to test the sensitivity, specificity, precision, and accuracy of the assay. The ability to measure lipid-associated amphotericin B was also evaluated in preliminary studies. Analysis of reference samples containing amphotericin B yielded a traditional sigmoidal curve. The limits of detection were 0.15 to 156 micrograms/ml. The sensitivity of the assay was affected by light and temperature exposure. Assay specificity was altered only by the presence of nystatin, a polyene antifungal agent similar to amphotericin B. Intrarun (coefficient of variation = 3.0%) and interrun (coefficient of variation = 12.8%) coefficients of variation were calculated and were comparable to those in similar assays. The assay's correlation coefficient (r = 0.907) demonstrated a statistically significant correlation between the optical density of the sample and the concentration of drug in the sample. The amphotericin B ELISA's ease, precision, and overall accuracy suggest that this assay could be used for assessments of serum amphotericin B concentrations. Multiple research questions concerning the role of serum amphotericin B concentrations in toxicity and efficacy have gone unanswered because of the labor-intensive nature of the assays which have been available to date. The ability to easily and rapidly measure 40 duplicate samples containing amphotericin B should also prove to be a distinct advantage for clinical research or reference laboratories in addressing these questions.
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Spinner, Sabine, Georg Stöffler, and Ernst Fink. "Quantitative enzyme-linked immunosorbent assay (ELISA) for hirudin." Journal of Immunological Methods 87, no. 1 (February 1986): 79–83. http://dx.doi.org/10.1016/0022-1759(86)90346-7.
Dillen, L., J. De Block, L. Van Lear, and W. De Potter. "Enzyme-linked immunosorbent assay for chromogranin A." Clinical Chemistry 35, no. 9 (September 1, 1989): 1934–38. http://dx.doi.org/10.1093/clinchem/35.9.1934.
Abstract This is an enzyme-linked immunosorbent assay (ELISA) for determining chromogranin A (CGA) with use of a monoclonal antibody. CGA was isolated from bovine chromaffin granules. The analytical ELISA procedure for bovine CGA was developed and optimized. Typical standard curves ranged from 500 pg to 500 ng of CGA. We then studied human plasma CGA-immunoreactivity as measured by this assay. The curve for dilutions of human plasma paralleled the standard curve for bovine CGA. The intra-assay coefficient of variation for determination of human plasma CGA was 4.56%, indicating that reliable determinations can be performed for human plasma. However, further study revealed the presence of two CGA-immunoreactive substances in human plasma, one of which corresponds to the native CGA. The nature of the second immunoreactive substance still remains unknown. Nevertheless the measured CGA concentrations (ranging from 0.19 to 0.35 mg/L) in plasma are comparable with previously reported values.
Дисертації з теми "Psychosocial Enzyme-linked immunosorbent assay (ELISA)":
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Nicholas, Lionel John. "The development of a university-based sex counselling programme in the age of AIDS." University of the Western Cape, 1993. http://hdl.handle.net/11394/8447.
Philosophiae Doctor - PhD The sexual behaviours, attitudes, beliefs and communication of 1896 black first-year university students were examined by means
of a structured questionnaire for their contribution to the development of a university-based sex counselling programme. The areas of sexuality investigated included intra-familial communication about contraception and sexuality, belief in sex myths, knowledge of and myths about AIDS and the manner of acquisition of sex knowledge. The results of this study are consistent in reflecting much greater deficits in the knowledge of respondents about sexuality than encountered in the literature. Statistically significant gender differences were found for
intra-familial communication about contraception, prejudice towards AIDS victims, knowledge of the modes of HIV infection, prejudice towards homosexuals, belief in myths about sexuality, age at which sex information was acquired, the preferred source of information about sexuality, attitude towards pre-marital intercourse, experience of pre-marital intercourse, belief about the acceptability of abortion, experience of pre-marital intercourse and worry about masturbation. No gender differences were found for belief in myths about high-risk AIDS infection, exposure to sex information within educational institutions and approval of sex education. The statistically significant gender differences which were found for most of the questionnaire items reflect the different sexual socialization experiences of respondents. Male and female students may therefore require counselling interventions geared to their respective needs Concern about AIDS has become central to university student sexual behaviour as well as protection against rape and sexual harassment and male responsibility for contraception. All campus counsellors will eventually experience the impact of AIDS and other sexually·transmitted diseases in their sessions with clients. Sexual harassment, rape, contraceptive failure and abortion will also increasingly impact on counselling sessions and require the university-based counsellor's involvement in broader university-wide prevention programmes as well as group based interventions. The development of a university-based sex counselling programme requires comprehensive interventions ranging from individual
counselling to human sexuality courses. An awareness of the high profile sexuality problems as perceived by students, is essential for the development of preventive programmes at the group and academic class level as well as at the level of inf luencing uni versi ty policy. Knowledge of the merits of different theoretical positions and interventions for particular sexual problems is crucial for counselling intervention or referral. A systemic model of intervention for sexuality problems is proposed. The task of university-based sex counselling programmes is made more onerous by the paucity and ineffectiveness of sex information students are exposed to, the lack of sex education
in the schools and the inadequate quality and degree of intrafamilial communication about sexuality. A significant proportion of respondents engage in pre-marital sexual intercourse without the benefit of adequate sex knowledge. The results of this study emphasize the need for research on the sexuality of, black South Africans, the particular vulnerabilities of first-year university students to sexuality problems and the
dire need for structured sex education programmes at school as well as university.
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Kim, In Soo. "A quantitative enzyme linked immunosorbent assay for polychlorinated biphenyls in transformer oil." Thesis, Cranfield University, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.323838.
Leung, Sau-man Sally. "Serodiagnostic utility of an ELISA assay based on a recombinant antigen MP1 of penicillium marneffei." Hong Kong : University of Hong Kong, 2001. http://sunzi.lib.hku.hk/hkuto/record.jsp?B2337309X.
Lupica, Samuel J. "Nitrate Toxicity to Common Carp Measured Noninvasively by Novel Enzyme-linked Immunosorbent Assay for Cortisol." University of Toledo Health Science Campus / OhioLINK, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=mco1226956326.
梁秀雯 and Sau-man Sally Leung. "Serodiagnostic utility of an ELISA assay based on a recombinant antigen MP1 of penicillium marneffei." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2001. http://hub.hku.hk/bib/B31970096.
Sheehan, C. P. "The application of the enzyme-linked immunosorbent assay (ELISA) to ABH grouping in forensic science." Thesis, University of Surrey, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.381661.
Cheow, Lih Feng. "Development of an Enzyme-Linked ImmunoSorbent Assay (ELISA) with Enhanced Sensitivity in a Nanofluidic System." Thesis, Massachusetts Institute of Technology, 2009. http://hdl.handle.net/1721.1/55148.
Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 2009. Cataloged from PDF version of thesis. Includes bibliographical references (p. 66-68). Experimental studies were performed to evaluate the kinetics and equilibrium binding constants of biomolecules in nanofluidic channels. Binding events in the nanochannel were detected using electrical and fluorescence methods. We concluded that antibody-antigen binding constants in nanochannels were similar to experiments performed in microtiter plates at low antigen concentrations; however the bound fraction in nanochannels at high antigen concentration decreased due to steric hindrance. Binding kinetics in nanochannels was limited by convective transport of analytes, instead of diffusion or reaction. We also found that enzymatic reactions in nanochannels were very effective due to short diffusion length and high surface area to volume ratio. A bead based ELISA was developed to exploit the rapid binding reactions in the bulk and efficient enzymatic conversion in the nanochannels. Additionally, electrokinetic concentrators were integrated with multiplexed bead based ELISA to further improve the detection sensitivity of a sandwich immunoassay. by Lih Feng Cheow. S.M.
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Johnson, Raymond Camille Joseph. "Detection of nepoviruses by ELISA in tissue-cultured and field-grown grapevines." Thesis, University of British Columbia, 1988. http://hdl.handle.net/2429/27968.
The detection by serology of nematode-transmitted polyhedral viruses (nepoviruses) in grapevines is often unreliable. Nepoviruses were detected by enzyme-linked immunosorbent assay (ELISA) in tissue-cultured and field-grown grapevines. Nepovirus detection in in vitro plants (plantlets) was affected by virus distribution and growth room temperature. The reliability of virus detection in field-grown grapevines was improved when modified grinding buffers were used.
Arabis mosaic virus (AMV) was detected by ELISA, for the first time, in in vitro grapevines initiated from field-and screenhouse-grown plants throughout the summer. The virus was not reliably and repeatedly detected in in vitro plantlets grown at 25°C.
AMV and grapevine fanleaf virus (GFLV) distribution was not uniform throughout the plantlets. This distribution was affected by the culture room temperature. The best plant parts to sample for virus detection came from the zones of rapidly proliferating shoots. The viruses were sometimes not detected in samples taken from other tissues.
Growth room temperature had an important effect on virus detection in plantlets. The highest virus titres were found in plants growing at 15°C. Temperature increases in 5°C steps to 30°C reduced virus titre. AMV became undetectable in nearly all plantlets growing at 30°C for as little as 30 days. Growth at 30°C reduced ELISA absorbance values by 76% after 8 days and after 21 days the values were at 4% of pre-treatment levels. The virus titre dropped below detectable levels in most plantlets. AMV could not be detected in plantlets or rooted explants after being placed in a 30°C treatment for 2 months.
Tomato ringspot virus was detected by ELISA, for the first time, in in vitro grapevine plants. The virus was repeatedly detected in in vitro plants growing at 20°C.
Under the typical summer conditions experienced at Sidney, B.C., modifying the standard ELISA grinding buffer (0.01 M phosphate buffered saline, pH 7.4, 0.05% Tween-20, 0.2% ovalbumin, 2% polyvinylpyrrolidone) was essential for reliable detection of AMV or GFLV. AMV was reliably detected by ELISA in foliar samples from field or screenhouse plants throughout the summer when the grinding buffer was modified by increasing the pH to at least 8.2 and adding 1% nicotine or 0.15 M phosphate buffered saline. The most reliable results with GFLV were obtained with the nicotine enhanced buffers.
In comparison, because of the increased workload associated with growing plants in vitro and the unreliable detection of viruses in these plants, it remained preferable to detect nepoviruses in field plants by ELISA. Land and Food Systems, Faculty of Graduate
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Östling, Jeanette. "An Approach to Improve the Detection System of a Diagnostic Enzyme-Linked Immunosorbent Assay." Thesis, KTH, Skolan för bioteknologi (BIO), 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-214620.
Orchard, Robert Graham. "A flow-through enzyme-linked immunoassay for progesterone." The University of Waikato, 2007. http://hdl.handle.net/10289/2277.
Bovine reproductive performance is one of the most important factors influencing dairy farm profitability. Present-day techniques for oestrus- and pregnancy-detection are unreliable and labour-intensive. Although measuring milk-progesterone at regular intervals allows the fertility status of a cow to be determined reliably, the labour cost of collecting and analysing samples is prohibitive. This project aimed to develop a progesterone sensing system that could be automated and integrated with the milking unit, thus minimising labour costs. The proposed system involved mixing the milk sample with an enzyme-antibody conjugate and then passing the sample through a column containing immobilised progesterone. Any progesterone in the milk would inhibit conjugate binding to the column. An enzyme substrate would then flow through the column and bound conjugate would be detected as a colour change at the column's outlet. Periodate-coupling was used to attach horseradish peroxidase enzyme to anti-progesterone antibody, and progesterone-3-carboxymethyloxime was immobilised on the polystyrene bead surface using amine-coupling. Both techniques are widely used. Initial experiments attempted to verify the success of these two reactions simultaneously, whereas later experiments focused on the bead-coating. Beads were suspended in a specially-constructed syringe and the antibody activity of the eluted solution was measured by SPR. However, a combination of non-specific binding and antibody stability and activity issues meant neither reaction was conclusively verified. Many trials were done to investigate how to overcome the problems encountered but a suitable, workable procedure was not developed. Despite poor progress, the problems encountered did not undermine the project's potential. There remains optimism of developing an on-line method if research were to continue.
Sánchez-Vizcaíno, J. M. Enzyme immunoassay techniques, ELISA, in animal and plant diseases. 2nd ed. Paris, France: Office international des épizooties, 1987.
Darcel, Colin. A collection of essays on comparative medicine, and a bibliography of references to the ELISA test. Lethbridge, Alberta: Palliser Animal Health Laboratories, 1996.
Darcel, Colin. A collection of essays on comparative medicine: And a bibliography of references to the ELISA test. Lethbridge, Alberta, Canada: Palliser Animal Health Laboratories, Ltd., 1996.
O'Connor, Glenda. Use of ELISA for monitoring bacterial kidney disease in naturally spawning chinook salmon. Salem, Or: Oregon Dept. of Fish and Wildlife, 2006.
Konstantinou, George N. "Enzyme-Linked Immunosorbent Assay (ELISA)." In Methods in Molecular Biology, 79–94. New York, NY: Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-6925-8_7.
Gooch, Jan W. "Enzyme-Linked Immunosorbent Assay (ELISA)." In Encyclopedic Dictionary of Polymers, 891. New York, NY: Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4419-6247-8_13676.
Crowther, John R. "Enzyme- Linked Immunosorbent Assay (ELISA)." In Springer Protocols Handbooks, 595–617. Totowa, NJ: Humana Press, 1998. http://dx.doi.org/10.1007/978-1-59259-642-3_46.
Walker, John M. "The Enzyme Linked Immunosorbent Assay (ELISA)." In Techniques in Molecular Biology, 82–97. Boston, MA: Springer US, 1987. http://dx.doi.org/10.1007/978-1-4615-9799-5_4.
Garvey, Justine S., Dafydd G. Thomas, and Harry J. Linton. "Enzyme-Linked Immunosorbent Assay (ELISA) for Metallothionein." In Experientia Supplementum, 335–42. Basel: Birkhäuser Basel, 1987. http://dx.doi.org/10.1007/978-3-0348-6784-9_31.
Butler, J. E., J. H. Peterman, and T. E. Koertge. "The Amplified Enzyme-Linked Immunosorbent Assay (a-ELISA)." In Enzyme-Mediated Immunoassay, 241–76. Boston, MA: Springer US, 1985. http://dx.doi.org/10.1007/978-1-4684-5012-5_14.
Alhabbab, Rowa Yousef. "Enzyme Immunoassay (EIAs) and Enzyme-Linked Immunosorbent Assay (ELISA)." In Techniques in Life Science and Biomedicine for the Non-Expert, 83–95. Cham: Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-77694-1_12.
Тези доповідей конференцій з теми "Psychosocial Enzyme-linked immunosorbent assay (ELISA)":
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Sugihara, T., J. Takamatsu, T. Kamiya, H. Saito, K. Kimata, and K. Kato. "A SENSITIVE ENZYME-LINKED IMMUNOSORBENT ASSAY(ELISA) FOR SERUM LAMININ." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643554.
Laminin, a large glycoprotein, is a major and specific component of basement membrane. There were little or no circulating laminin in normal persons, although some recent reports have showed increased values in various diseases(ex. diabetes mellitus and liver disease) by a radioimmunoassay(RIA) using laminin fragment. The minimum detectable sensitivity of RIA was reported to be 20 ng/ml of serum sample. We describe here a more sensitive immunoassay system, and also the concentrations of laminin in sera from healthy subjects and patients. A sandwich ELISA method for measurement of laminin was established by use of purified antibodies to mouse laminin. The assay system consisted of poly-stylene balls with immobilized antibody F(ab’)2 fragments and the same antibody Fab1 fragments labeled with β-D-galactosidase from E.Coli. The assay was highly sensitive and can detect as small as 0.5 ng/ml of serum laminin.Coefficients of variation in within-run and between-run precision studies for serum laminin were good. Serum laminin levels in healthy subjects of various ages ranged from 1.5 to 3.9 ng/ml(n=60). Fifty eight patient sera (collagen disease(n=18), hepatic disease(n=20), and renal disease (n=20)) were examined. Significant differences between the normal sera and 3 diseases sera were observed as shown belowIt is concluded that circulating laminin apparently exists in normal persons and there are higher laminin level in some diseases which appears to involve basement membrane-rich organ
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Lee, Wen-Wai, Kuan-Ju Tseng, and Ju-Nan Kuo. "PDMS microlens fabricated for compact disk enzyme-linked immunosorbent assay (CD-ELISA) applications." In 2010 5th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS 2010). IEEE, 2010. http://dx.doi.org/10.1109/nems.2010.5592184.
MacGregor, I. R., N. A. Booth, N. R. Hunter, and B. Bennet. "QUANTIFICATION OF ENDOTHELIAL PLASMINOGEN ACTIVATOR INHIBITOR (PAI-1) ANTIGEN BY AN ENZYME LINKED IMMUNOSORBENT ASSAY (ELISA)." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644450.
An ELISA to measure PAI-1 antigen has been developed using PAI-1 purified from human endothelial cell conditioned medium and a monospecific antiserum raised against it in the rabbit. Test and standard samples diluted in assay buffer containing non-immune rabbit serum were incubated in microplate wells coated with anti-PAI-1 IgG. Then biotinylated anti-PAI-1 IgG was added to the wells followed by a streptavidin-biotinylated horseradish peroxidase complex. Tetramethylbenzidine was used as substrate and the optimised ELISA had a detection limit of 0.2 ng PAI-1 ml-1 sample, using purified PAI-1 of known concentration as a standard for the assay.PAI-1 antigen was readily detectable in human plasmas and higher concentrations were invariably detected in the corresponding whole blood sera.α2antiplasmin and antithrombin III which have been shown to have high degrees of sequence homology with PAI-J were undetectable in the ELISA at concentrations of 10 ug ml-1 . Sera from baboon and Rhesus and Cynomologus monkeys exhibited partial cross reactivity in the ELISA while no crossreactivities were observed with a wide range of non-primate sera that included dog, goat, cow, horse, pig and rat.A variety of human cell cultures were assayed for PAI-1 antigen. Endothelial cells from umbilical cord veins and arteries and from adult sapgenous veins secreted, typically,1-2 ug PAI-1 24-1 hr per 10 cells. This represented approximately 2-4% of the total secreted protein. Detectable but considerably lower levels of PAI-1 were secreted by keratinocytes and fibroblasts as well as a lung carcinoma cell line A549.No PAI-1 was detected in media conditioned by two melanoma cell lines, a breast carcinoma cell line MCF7 or a monocytic leukaemia JIII. The ELISA proved suitable for monitoring altered cellular PAI-1 metabolism, and in conjunction with functional assays, for investigating changes in PAI-1 specific activity.
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Grøndahl-HANSEN, J., N. Agerlin, L. S. Nielsen, and K. Danø. "SENSITIVE AND SPECIFIC ENZYME-LINKED IMMUNOSORBENT ASSAY FOR UROKINASE-TYPE PLASMINOGEN ACTIVATOR IN HUMAN PLASMA." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644425.
An enzyme-linked immunosorbent assay (ELISA) was developed for the measurement of human urokinase-type plasminogen activator (u-PA) in plasma and serum. Microtiter plates were coated with a monoclonal antibody and incubated with standard or sample. Bound u-PA was quantitated with polyclonal antibodies conjugated with biotin, followed by avidin-peroxidase. The assay was 10-fold as sensitive as other previously reported ELISAs, the detection limit being approximately 1 pg of u-PA in a volume of 100 μl with a linear dose-response up to 15 pg of u-PA. The assay detected active u-PA and its inactive proenzyme form equally well and the recovery of both forms was higher than 90% in plasma. A variety of structurally related proteins, including t-PA, were tested, but no reaction with proteins other than u-PA and its amino-terminal degradation product were observed. The intra-assay and inter-assay coefficients of variation for determination of u-PA in plasma were 7.6% and 8.4%, respectively. The assay was equally applicable to serum. The values obtained with plasma and serum were similar, and the results were not affected by small variations in the preparation of the samples. The ELISA was used to measure the concentration of u-PA in plasma from 34 healthy donors. The mean values for u-PA in plasma from healthy donors was 1.1 ng/ml ± 0.3 ng/ml (SD) (range 0.6 - 1.5 ng/ml). No significant differences were found between men and women and no correlation between u-PA concentration and age could be demonstrated.The mean u-PA concentration in plasma from healthy donors obtained in this study is substantially lower than that reported by others. This might be due to different methods of determination of the protein content of the standard preparations or to differences in the specificity of the assays.
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Metwali, Nervana, and Peter S. Thorne. "Development Of An Enzyme-Linked Immunosorbent Assay (ELISA) For Bacterial Peptidoglycan In Air And Dust Samples." In American Thoracic Society 2010 International Conference, May 14-19, 2010 • New Orleans. American Thoracic Society, 2010. http://dx.doi.org/10.1164/ajrccm-conference.2010.181.1_meetingabstracts.a4643.
Takehara, H., K. Miyazawa, T. Noda, K. Sasagawa, T. Tokuda, S. H. Kim, R. Iino, H. Noji, and J. Ohta. "A CMOS Image Sensor Having Stacked Photodiodes for Lensless Observation System of Digital Enzyme-linked Immunosorbent Assay (ELISA)." In 2013 International Conference on Solid State Devices and Materials. The Japan Society of Applied Physics, 2013. http://dx.doi.org/10.7567/ssdm.2013.g-4-4.
Yamamura, Yuki, Tomoaki Iwai, and Yutaka Shokaku. "ELISA Analysis of Latex Allergens on Wear Particles of Natural Rubber During Rolling-Sliding Contact." In ASME/STLE 2009 International Joint Tribology Conference. ASMEDC, 2009. http://dx.doi.org/10.1115/ijtc2009-15237.
The wear particles generated from a tire model made of vulcanized natural rubber were analyzed using ELISA (Enzyme-Linked Immunosorbent Assay). Antibodies to 4 different allergens were used to analyze the extracted proteins from the wear particles. Latex allergens were detected when the slip ratio was large. However, no clear relationship between the latex allergen content extracted from the wear debris and either the average size or the number of wear particles was observed.
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Czerchaujski, L., V. Hornsey, C. Prowse, and H. Bessos. "CROSS-REACTIV7E Fl/III :C IN THE RABBIT: A POTENTIAL ANIMAL MODEL FOR FUIII:C STUDIES." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644036.
A one step Enzyme-linked immunosorbent assay (ELISA) incorporating two anti-human FVIII :Ag monoclonal antibodies (MAbs) was used to determine FUIIIrAg in rabbits. Initial examinations showed the presence of cross-reactive FUIII:C in rabbit serum, plasma, and homogenates of normal rabbit liver, lung and spleen. Detailed investigation of normal rabbit plasma, and plasma depleted with Sephacryl S1000-anti FVIII :C MAbs and irrelevant MAbs (as control) using the ELISA, a chromogenic Fl/III:C activity assay, an immunoradiometric assay (IRMA) using human antibody, and fast protein liquid chromatography (FPLC) showed conclusively that the ELISA was specific for FVIII:C. The cross reactive FVIII:Ag was found to be most prominent in rabbit plasma, followed by serum, liver, lung and spleen. The ELISA should enable potential use of the rabbit as an animal model for FVIII:C studies, such as the enhancement of homologous FVIII:C with drugs or following tissue transplantation.
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Dutta, Debashis, and Naoki Yanagisawa. "Microfluidic Devices for Enhancing the Sensitivity of ELISA Methods." In ASME 2011 9th International Conference on Nanochannels, Microchannels, and Minichannels. ASMEDC, 2011. http://dx.doi.org/10.1115/icnmm2011-58284.
Enzyme-linked immunosorbent assays (ELISA) are critically important tools in biological research, allowing the presence and concentrations of a wide variety of key biochemical intermediates to be determined. While the signal amplification that is the core advantage of ELISA methods is impressive, it is nevertheless the case that it is insufficient for some particularly demanding challenges in terms of sensitivity, assay time, or sample size. In this paper, we discuss three different approaches developed in our laboratory that can improve the sensitivity of ELISA methods by 2–3 orders of magnitude. Two of these approaches have been shown to reduce the minimum detectable concentration of the target analyte in the system through trapping of the analyte species and the enzyme reaction product around a semi-permeable membrane. The third approach, on the other hand, focuses on reducing the sample volume requirement in these assays by implementing multiplex ELISA methods in a single microfluidic channel using the same enzyme label. This multiplex technique relies on the slow diffusion of the enzyme reaction product across adjacent assay segments for accurate quantitation and has been demonstrated to have a limit of detection substantially better than that of commercial microtiter plates. We believe the combination of these approaches could significantly extend the applicability of the ELISA technique to more challenging assays than is currently possible.
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Takehara, Hironari, Kazuya Miyazawa, Toshihiko Noda, Kiyotaka Sasagawa, Takashi Tokuda, Soo Hyeon Kim, Ryota Iino, Hiroyuki Noji, and Jun Ohta. "A CMOS image sensor with low fixed pattern noise suitable for lensless observation system of digital enzyme-linked immunosorbent assay (ELISA)." In 2013 IEEE International Meeting for Future of Electron Devices, Kansai (IMFEDK). IEEE, 2013. http://dx.doi.org/10.1109/imfedk.2013.6602227.
Звіти організацій з теми "Psychosocial Enzyme-linked immunosorbent assay (ELISA)":
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Occurrence and Distribution of Pesticides in the St. Lucie River Watershed, South-Central Florida, 2000-01, Based on Enzyme-Linked Immunosorbent Assay (ELISA) Screening. US Geological Survey, 2003. http://dx.doi.org/10.3133/wri024304.
