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Relevant bibliographies by topics / 2-dimensional electrophoresis

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Academic literature on the topic '2-dimensional electrophoresis'

Author: Grafiati

Published: 2 June 2025

Last updated: 31 July 2025

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Journal articles on the topic "2-dimensional electrophoresis"

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Lehr, Stefan, and Reiner Westermeier. "2-dimensional gel electrophoresis reloaded." Archives of Physiology and Biochemistry 119, no. 3 (2013): 93. http://dx.doi.org/10.3109/13813455.2013.812122.

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Akhremko, Anastasiya, Ekaterina Romanovna Vasilevskaya, and Liliya Fedulova. "Adaptation of two-dimensional electrophoresis for muscle tissue analysis." Potravinarstvo Slovak Journal of Food Sciences 14 (August 28, 2020): 595–601. http://dx.doi.org/10.5219/1380.

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It is important to understand the molecular mechanisms that take place in muscle tissues and to predict meat quality characteristics. One of the most popular methods is two-dimensional electrophoresis, which allows us to visualize, share and identify different molecules, including meat proteins. However, the standard conditions of this method are not universal for all types of raw material, so the authors suggest a new variation of two-dimensional electrophoresis for muscle tissue analysis. Samples were tested by the classical version of isoelectric focusing (cathode buffer in the top and anod

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Kusari, J., and P. Roy. "Molecular and genetic comparisons of two serotypes of epizootic hemorrhagic disease of deer virus." American Journal of Veterinary Research 47, no. 8 (1986): 1713–17. https://doi.org/10.2460/ajvr.1986.47.08.1713.

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SUMMARY The virus-specific double-stranded genome RNA of 2 serotypes of epizootic hemorrhagic disease of deer virus (ehdv) was evaluated by use of coelectrophoresis in polyacrylamide and agarose gel systems. The molecular weights of virion RNA segments were 0.32 to 2.57 × 106 for ehdv-1 and 0.33 to 2.54 × 106 for ehdv-2. Seven of 10 double-stranded RNA segments of the 2 serotypes had different electrophoretic mobilities in the polyacrylamide-gel electrophoresis system. Although the individual RNA segments of each serotype contained unique RNA sequences determined on the basis of 2-dimensional

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Parent, Jean-Guy, Richard Hogue, and Alain Asselin. "Glycoproteins, enzymatic activities, and b proteins in intercellular fluid extracts from hypersensitive Nicotiana species infected with tobacco mosaic virus." Canadian Journal of Botany 63, no. 5 (1985): 928–31. http://dx.doi.org/10.1139/b85-123.

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Intercellular fluid b proteins from hypersensitive Nicotiana tabacum L. cv. Xanthi-nc and N. sylvestris Speg. and Comes infected with tobacco mosaic virus were compared by two-dimensional (2-D) polyacrylamide gel electrophoresis. Except for missing bands b2, b6a, b6b, and b7b, the overall 2-D electrophoretic pattern of N. sylvestris intercellular fluid proteins was similar to the one observed with 'Xanthi-nc' tobacco. Intercellular proteins were also studied by chromatography on con-canavalin A. Glycoproteins corresponding to b6a and b7a proteins of N. tabacum and the [Formula: see text] analo

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Tsai, Shuo-Wen, Michael Loughran, and Isao Karube. "Development of a microchip for 2-dimensional capillary electrophoresis." Journal of Micromechanics and Microengineering 14, no. 12 (2004): 1693–99. http://dx.doi.org/10.1088/0960-1317/14/12/014.

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Jeng, Robert S., Shiyuan Yu, and Morris Wayman. "Isoenzyme and protein patterns of pentose-fermenting yeasts." Canadian Journal of Microbiology 33, no. 11 (1987): 1017–23. http://dx.doi.org/10.1139/m87-179.

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Soluble proteins were extracted from the vegetative cells of four pentose-fermenting yeasts, Candida shehatae, Pichia stipitis, R-1, and R-2, the R strains being of uncertain taxonomy, while the other two are culture collection yeasts. Isoenzyme patterns, protein patterns, and two-dimensional polypeptide mapping of these four strains were compared by polyacrylamide gel electrophoresis. The two R strains showed great similarity in two-dimensional polypeptide mapping, the pattern of sodium dodecyl sulfate – polyacrylamide gel electrophoresis, isoelectrofocusing, and isoenzymes, and may be one sp

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Tokur, Bahar, and Koray Korkmaz. "Electrophoretic Methods for Identifying the Species of Seafood and Its Derivatives." Food Bulletin 2, no. 2 (2023): 61–70. http://dx.doi.org/10.61326/foodb.v2i2.121.

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The identification of the species of seafood and their products, whether they are fresh or cooked, is one of the key concerns of food regulations in many countries that have a significant intake of seafood. In point of fact, a commercial fraud happens when a species that is less value is intentionally substituted for a species that is more valuable, and a sanitary fraud takes place when a product that is potentially harmful is introduced into the market. A primary responsibility of veterinary inspection of seafood products is the detection of harmful species with the aim of removing them from

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Shelton, G. Diane, Everett Bandman, and George H. Cardinet. "Electrophoretic comparison of myosins from masticatory muscles and selected limb muscles in the dog." American Journal of Veterinary Research 46, no. 2 (1985): 493–98. https://doi.org/10.2460/ajvr.1985.46.02.493.

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SUMMARY Myosins from canine limb muscles (triceps brachii [medial and long heads], anconeus, and extensor carpi radialis) were compared biochemically with myosins from canine masticatory muscles (temporalis and masseter). Compared with the limb muscles, the temporalis and masseter muscles had: a unique myosin isoform pattern as determined by nondenaturing pyrophosphate gel electrophoresis; unique light chains as determined by 1-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis, 2-dimensional gel electrophoresis, and peptide mapping; and a unique heavy chain as determined by

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Guo, X., C. Zhao, F. Wang, et al. "Investigation of Human Testis Protein Heterogeneity Using 2-Dimensional Electrophoresis." Journal of Andrology 31, no. 4 (2010): 419–29. http://dx.doi.org/10.2164/jandrol.109.007534.

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Hochstrasser, D., V. Augsburger, T. Pun, D. Weber, C. Pellegrini, and A. F. Muller. ""High-resolution" mini-two-dimensional gel electrophoresis automatically run and stained in less than 6 h with small, ready-to-use slab gels." Clinical Chemistry 34, no. 1 (1988): 166–70. http://dx.doi.org/10.1093/clinchem/34.1.166.

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Abstract Although two-dimensional (2-D) gel electrophoresis is one of the most powerful techniques for analyzing protein mixtures, its application in routine clinical laboratories is currently limited, because it is time-consuming, complex, and relatively expensive. Here we describe a method for automatically running and staining "high-resolution" mini 2-D electrophoresis gels in less than 6 h, by using "ready-to-use" slab gels and a PhastSystem electrophoresis apparatus. We present 2-D gel electrophoretograms of 25 nL of plasma, as well as their automatic computer analysis. For comparison, a

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Dissertations / Theses on the topic "2-dimensional electrophoresis"

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Yao, Mingyi. "Proteomic studies of Pseudomonas putida KT2440 in carcinogenicity screening via 2-dimensional gel electrophoresis /." Online version of thesis, 2005. https://ritdml.rit.edu/dspace/handle/1850/1118.

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Misztal, David Richard Biotechnology &amp Biomolecular Sciences Faculty of Science UNSW. "Identification of cellular changes associated with increased production of human follicle stimulating hormone in a recombinant Chinese hamster ovary cell line." Publisher:University of New South Wales. Biotechnology & Biomolecular Sciences, 2008. http://handle.unsw.edu.au/1959.4/41326.

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A proteomics approach was used to identify proteins potentially implicated in the cellular response concurrent with elevated production levels of human follicle stimulating hormone in a recombinant Chinese hamster ovary cell line (Darren cells), using zinc and sodium butyrate in the production media to increase expression. To this end, 2-dimensional gel electrophoresis (2-DGE) was utilized. Firstly, several aspects of 2-DGE were developed for this investigation. Gel drying conditions were optimized, and a glycine-free blotting method is described which achieved greater efficiency in rapid tran

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Trein, Cristina Rodrigues. "Eletroforese bidimensional em gel de poliocrilamida do plasma seminal equino e a sua relação com a congelabilidade do sêmen." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2012. http://hdl.handle.net/10183/39292.

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O objetivo deste estudo foi avaliar o perfil proteico do plasma seminal equino utilizando eletroforese bidimensional de gel de acrilamida (2D-PAGE) e determinar se algumas das proteínas presentes estavam relacionadas com a congelabilidade do sêmen. O plasma seminal foi coletado de dez garanhões, de alta e baixa congelabilidade de sêmen, provenientes do Haras Estatal da Baixa Saxônia, na cidade de Celle, na Alemanha e rotineiramente utilizados em programas de inseminação artificial. Vinte e cinco bandas proteicas foram encontradas nos géis bidimensionais (12%) e sete delas foram identificadas p

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Apraiz, Larrucea Itxaso. "Development and application of a proteomic approach to the assessment of pollution in the marine environment." Doctoral thesis, Stockholms universitet, Institutionen för biokemi och biofysik, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-26150.

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Today, assessment of the health of coastal waters is recognized as being important for both the conservation of nature and well-being of humans. Anthropogenic pollution has been the focus of extensive research for some time and a variety of programs for the monitoring and assessment of environmental pollution have been developed. Determination of the levels of pollution in sensitive ‘sentinels’ such as mussels, allows monitoring of these levels in a given area over a prolonged period of time. Furthermore, the biological effects of pollution are reflected in a series of biomarkers, none of whic

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Coaker, Gitta Laurel. "Genetic and biochemical characterization of resistance to bacterial canker of tomato caused by Clavibacter michiganensis subsp. michiganensis." The Ohio State University, 2003. http://rave.ohiolink.edu/etdc/view?acc_num=osu1069188955.

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Amelina, Hanna. "Proteomics in biomarker research : Insights into the effects of aging and environment on biological systems." Doctoral thesis, Stockholms universitet, Institutionen för biokemi och biofysik, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-56483.

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Proteomics is the global analysis of proteins that covers a broad range of technologies aimed at determining the identity and quantity of proteins expressed in the cell, their three-dimensional structure and interaction partners. In contrast to genome, proteome reflects more accurately on the dynamic state of the cell, tissue, or an organism. Therefore much is expected from proteomics to yield better disease markers for early diagnosis and therapy monitoring, as well as biomarkers that would indicate environmental exposure or provide prediction of biological age. In this thesis, I have develop

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Qiu, Linghua. "Differentially Expressed Proteins in the Pancreas of Diabetic Mice." Ohio University / OhioLINK, 2005. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1125866599.

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ARBA, MORENA. "Caratterizzazione proteomica di fluidi e tessuti in diverse condizioni fisio-patologiche." Doctoral thesis, Università degli Studi di Cagliari, 2015. http://hdl.handle.net/11584/266808.

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This thesis has been focused on the proteomic characterization of human saliva from donors of different ages, starting from birth up to adult age, and pediatric brain tumor tissues. The first study has been performed in order to compare the acid-insoluble fraction of saliva from preterm with at-term newborns and adults and establish if differences exist. In the second study medulloblastoma and pilocytic astrocytoma pediatric brain tumor extracts have been compared. In both studies 2- DE analysis was coupled with high resolution tandem mass spectrometry (MS/MS). The proteomic characteriz

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Kierul, Kinga. "Comprehensive proteomic study of Bacillus amyloliquefaciens strain FZB42 and its response to plant root exudates." Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, 2013. http://dx.doi.org/10.18452/16805.

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Bacillus amyloliquefaciens FZB42 ist ein frei lebendes Bakterium, das Pflanzenwurzeln besiedelt und das Pflanzenwachstum durch viele verschiedene Wirkmechanismen anregt. In dieser Arbeit wurden die molekularen Grundlagen dieser positiven Wirkungen, die dieses „Pflanzenwachstum fördernde Rhizobakterium“ (PGPR) auf seine Wirte ausübt, untersucht. Um den gegenseitigen Austausch von B. amyloliquefaciens und seinen Wirtspflanzen zu entschlüsseln, wurden umfangreiche Proteomstudien durchgeführt. Es wurden Referenzkarten der extrazellulären und zytosolischen Proteinfraktionen erstellt. Die größte A

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Rajagopal, Meena Uma. "METHODS DEVELOPMENT AND APPLICATION OF TWO-DIMENSIONAL GEL ELECTROPHORESIS AND MASS SPECTROMETRY IN PROTEOMICS." UKnowledge, 2006. http://uknowledge.uky.edu/gradschool_diss/292.

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The development of a highly sensitive ruthenium-based fluorescent staining solution isdescribed in this dissertation. The in-house synthesized ruthenium complex (RuMS)containing both sulfonated and non-sulfonated ligand has detection limit of 1 ng ofprotein that is better than colloidal coomassie, silver and ruthenium complex containingall sulfonated ligands (RuBPS). RuMS stain has 100-fold dynamic range and does notinterfere with subsequent mass spectral identification of proteins. The capability of inhousesynthesis of the staining solution makes it a viable cost-effective alternative to thee

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Books on the topic "2-dimensional electrophoresis"

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1991), International Meeting on Two-Dimensional Electrophoresis (London. 2-D PAGE '91: Proceedings of the International Meeting on Two-Dimensional Electrophoresis, London, 16th-18th July, 1991. Department of Cardiothoracic Surgery, National Heart and Lung Institute, 1991.

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Endler, A. T. 2 Dimensional Electrophoresis: Proceedings of the International 2 Dimensional Electrophoresis Conference, Vienna, November 1988. Vch Pub, 1989.

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1936-, Dunn Michael J., and National Heart and Lung Institute. Department of Cardiothoracic Surgery., eds. 2-D Page '91: Proceedings of the International Meeting on Two-Dimensional Electrophoresis, London, 16th-18th July, 1991. Department of Cardiothoracic Surgery, National Heart and Lung Institute, 1991.

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Posch, Anton. 2D PAGE : Sample Preparation and Fractionation: Volume 2. Humana Press, 2010.

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Posch, Anton. 2D PAGE: Sample Preparation and Fractionation: Volume 2 (Methods in Molecular Biology). Humana Press, 2008.

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Marengo, Emilio, and Elisa Robotti. 2-D PAGE Map Analysis: Methods and Protocols. Springer New York, 2016.

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Marengo, Emilio, and Elisa Robotti. 2-D page map analysis: Methods and protocols. 2016.

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Book chapters on the topic "2-dimensional electrophoresis"

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Bizios, Nick. "Radiolabeling of Eukaryotic Cells and Subsequent Preparation for 2-Dimensional Electrophoresis." In Springer Protocols Handbooks. Humana Press, 2009. http://dx.doi.org/10.1007/978-1-59745-198-7_31.

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Zhang, Daohai, and Evelyn Siew-Chuan Koay. "Analysis of Laser Capture Microdissected Cells by 2-Dimensional Gel Electrophoresis." In Methods in Molecular Biology™. Humana Press, 2008. http://dx.doi.org/10.1007/978-1-59745-117-8_5.

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Berkelman, Tom. "Removal of Interfering Substances in Samples Prepared for Two-Dimensional (2-D) Electrophoresis." In Methods in Molecular Biology™. Humana Press, 2008. http://dx.doi.org/10.1007/978-1-60327-064-9_5.

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Pascali, V. L., E. d’Aloja, and M. Dobosz. "Classification of alphal antitrypsin phenotypes by high-resolution two-dimensional electrophoresis (2-D PAGE)." In Advances in Forensic Haemogenetics. Springer Berlin Heidelberg, 1988. http://dx.doi.org/10.1007/978-3-642-73330-7_36.

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Mettrick, Karla A., Georgia M. Weaver, and Ian Grainge. "Neutral–Neutral 2-Dimensional Agarose Gel Electrophoresis for Visualization of E. coli DNA Replication Structures." In Methods in Molecular Biology. Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-0323-9_5.

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Talorete, Terence P. N., Hiroko Isoda, and Kouji Nakamura. "Two-Dimensional Electrophoresis of Proteins from Human Intestinal Caco-2 Cells Exposed to the Known Xenoestrogen Nonylphenol." In Animal Cell Technology: Basic & Applied Aspects. Springer Netherlands, 2003. http://dx.doi.org/10.1007/978-94-017-0726-8_65.

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Baudin, Bruno. "Two-Dimensional Gel Electrophoresis (2-DE)." In Gel Electrophoresis - Principles and Basics. InTech, 2012. http://dx.doi.org/10.5772/38312.

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Heegaard, Niels H. H. "Capillary electrophoresis." In Protein-Ligand Interactions: hydrodynamics and calorimetry. Oxford University PressOxford, 2000. http://dx.doi.org/10.1093/oso/9780199637492.003.0007.

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Abstract Electrophoresis is the transport of charged molecules in solution by the action of an electrical field (1). Electrophoretic techniques are widely used to separate and characterize biomolecular mixtures and very high resolution may be achieved by the use of denaturing one- or two-dimensional gel methods. The separation efficiency in these techniques is normally achieved at the expense of analyte functions such as enzyme and ligand binding activities that are adversely affected by the denaturing conditions required. With the advent of capillary electro phoresis (CE) (2), however, it has

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McGregor, Emma, and Michael J. Dunn. "Two-dimensional gel electrophoresis (2-DE)." In Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics. John Wiley & Sons, Ltd, 2005. http://dx.doi.org/10.1002/047001153x.g302406.

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Ferrara, Pascual. "Sequencing of proteins isolated by one-or two-dimensional gel electrophoresis." In MHC. Oxford University PressOxford, 1998. http://dx.doi.org/10.1093/oso/9780199635559.003.0002.

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Abstract One-dimensional (1D), and especially two-dimensional (2D) polyacrylamide gel electrophoresis (PAGE), are generally considered as the highest resolving techniques for the analysis of complex protein mixtures. Both techniques have been extensively used for the study of cell protein changes in biological processes such as proliferation and differentiation (1). In 2D PAGE the power of electrophoresis analysis is enhanced when individual spots on the gel are identified. However, the identification of the spots is not a trivial task; several direct and indirect methods have been developed (

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Conference papers on the topic "2-dimensional electrophoresis"

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Cheung, K. H., H. Y. Lai, and Ji-Dong Gu. "Reduction of Chromate by Marine Bacillus Megaterium TKW3." In CORROSION 2006. NACE International, 2006. https://doi.org/10.5006/c2006-06521.

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abstract Hexavalent chromium (Cr6+) is an important corrosion inhibitor and is also highly toxic pollutant, which can be detoxified and precipitated through reduction to Cr3+. Bacillus megaterium TKW3 isolated from contaminated sediments was capable of reducing Cr6+ in concomitance with metalloids (Se4+, Se6+ and As5+). Notwithstanding the approximately 50% inhibition, it is firstly reported that bacteria reduced Cr6+ and Se4+ [to elemental Se] simultaneously. No significant difference was observed among electron donors (glucose, maltose and mannitol) on Cr6+ reduction of B. megaterium TKW3. T

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Rogers, M., J. Graham, and R. P. Tong. "2 Dimensional Electrophoresis Gel Registration Using Point Matching and Local Image-Based Refinement." In British Machine Vision Conference 2004. British Machine Vision Association, 2004. http://dx.doi.org/10.5244/c.18.59.

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Meyer, M., I. Schellenberg, B. Hofmann, and G. Vogel. "A GENETIC VARIANT OP PLATELET MEMBRANE GLYCOPROTEIN lb ASSOCIATED WITH A MILD BLEEDING DISORDER." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643510.

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Mild bleeding symptoms in a female patient were shown to be related to defective platelet function: Aggregation induced by ADP and PAP was decreased and platelet spreading was disturbed. Platelet membrane glycoproteins were analyzed by various electrophoretic procedures (SDS electrophoresis, nonreduced-reduced 2-dimensional and high resolution 2-dimensional electrophoresis). These studies revealed a decreased concentration of normal glycoprotein lb (GP Ib) and the appearance of an additional glycopeptide with an apparent Mr of 160.000 under reducing conditions. This component was strongly labe

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Fan, C., Z. Fu, Q. Su, J. Van Eyk, and RA Johns. "2-Dimensional Electrophoresis To Identify Downstream Molecules in HIMF Induced Signaling Pathway in Human Pulmonary Artery Smooth Muscle Cells." In American Thoracic Society 2009 International Conference, May 15-20, 2009 • San Diego, California. American Thoracic Society, 2009. http://dx.doi.org/10.1164/ajrccm-conference.2009.179.1_meetingabstracts.a1899.

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Panzer, S., I. Pabinger, R. Dudczak, et al. "PROTEIN S AND ANTICARDIOLIPIN ANTIBODIES IN PATIENTS WITH LUPUS ANTICOAGULANT WITH OR WITHOUT THROMBOSIS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644236.

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Arterial and venous thrombosis frequently occur in patients with lupus anticoagulant. We investigated in patients with lupus anticoagulant the possible association between protein S:Ag deficiency and/or anticardiolipin antibodies and a history of thrombosis. In 27 patients (8 with and 19 without a history of thromboembolic disease) free protein S:Ag was determined with immunoelectrophoresis in PEG 8000 precipitated plasma. Anticardiolipin antibodies were measured in 22 patients (10 with thromboembolism) by means of a solid phase radioimmunoassay.Free plasma protein S:Ag was normal or elevated

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Jackson, C. W., N. K. Hutson, S. A. Steward, and H. H. Edwards. "THE WISTAR-FURTH RAT: AN ANIMAL MODEL OF HEREDITARY MACRO THROMBOCYTOPENIA." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643925.

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The mechanisms which determine and regulate platelet size are unknown. By phase microscopy, we serendipitously observed that Wistar/Furth (W/F) rats had macrothrombocytopenia. In this study, we have characterized and compared platelets and megakaryocytes (MK) of W/F rats to those of Wistar (W), Long-Evans hooded (LE) and Sprague-Dawley (SD) rats. In addition, we have examined the mode of inheritance of this W/F rat platelet abnormality. Average platelet count of W/F rats was only 312 ± 57 x 103/mm3 compared to 1086 ± 68, 868 ± 91 and 926 ± 82 x 103/mm3 respectively for W, LE and SD rats. Mean

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Bahukudumbi, P., Michael A. Bevan, and Ali Beskok. "Self and Directed Colloidal Assembly on Patterned Electrodes." In ASME 2005 International Mechanical Engineering Congress and Exposition. ASMEDC, 2005. http://dx.doi.org/10.1115/imece2005-80628.

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Clustering of colloidal particles near an electrode surface during and after electrophoretic deposition has been reported in the literature [1, 2, 3, 4]. The aggregation of colloidal particles has made the precise assembly of two and three dimensional colloidal crystals possible. In this paper, we demonstrate the use of external electric fields to sensitively tune the interactions between colloidal particles to form ordered structures. The directed assembly of colloidal particles on patterned electrode surfaces is also investigated as a means of building three-dimensional nanostructures. Final

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Moroi, M., S. M. Jung, N. Yoshida, N. Aoki, and K. Tanoue. "THROMBASTHENIA WITH AN ABNORMAL PLATELET MEMBRANE GPIIb OF DIFFERENT MOLECULAR WEIGHT." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644741.

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We describe several individuals with abnormal platelet glycoprotein lib (GPIIb) of different molecular weight (MW), a novel defect in that previously reported thrombasthenics have not shown GPIIb molecules of abnormal MW. Patient 1 shows 2 bands of GPIIb (one with normal MW and one with abnormal MW) in SDS-PAGE (lectin and antibody staining). This patient has a mild bleeding tendency, and her platelets did not aggregate in response to ADP (2-10 μM) and had severely depressed collagen induced aggregation ,but responded normally to ristocetin. The MW of the abnormal GPIIb band was calculated to

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Reports on the topic "2-dimensional electrophoresis"

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Thongtan, Thananya, Poonlarp Cheepsunthorn, and Kiat Ruxrungtham. An analysis and studies expression of receptor molecule on microglia cells to inhibits infection of the cells from Japanese encephalitis virus : Research report (Year 2009). Chulalongkorn University, 2009. https://doi.org/10.58837/chula.res.2009.14.

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Japanese encephalitis virus (JEV), a mosquito-borne flavivirus, is a major cause of viral encephalitis in Asia. Even though the principle target cells for JEV in the central nervous system are neurons, the microglia is activated in response to JEV infection. This research aimed to investigate the relationship between JEV and microglial cells. The percentage of JEV infectivity in mouse microglial (BV-2) cell line at 8, 15 and 24 hr post infection was determined by flow cytometry. It was found that the percentage of infected cells were approximately 53.5, 71.3 and 83.6 respectively. The JEV bind

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