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Journal articles on the topic 'Bacterial preservation'

Author: Grafiati

Published: 4 June 2025

Last updated: 1 August 2025

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1

A.M., Jana, and Singh Pragya. "Bacterial Preservation." International Journal of Life Sciences and Technology (IJLST) ISSN:0974-5335 13, no. 1 (2020): 1–29. https://doi.org/10.5281/zenodo.4014767.

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Bacterial Preservation A.M. Jana<sup>1</sup> and Pragya Singh<sup>2</sup> 1 Director (R&D) & Department of Biotechnology, Vijayaraje Institute of Science and Management (VISM), Gwalior-474001, M.P., INDIA 2. Principal and Director VISM Group of Studies & Department of Biotechnology, Vijayaraje Institute of Science and Management (VISM), Gwalior-474001, M.P., INDIA amjana@rediffmail.com, pragyasingh797@gmail.com, vism_gwalior@rediffmail.com <strong>ABSTRACT</strong>: The present work pertains to preservation of bacterial culture using liquid nutrient media incorporated with a simple

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Martoni, Francesco, Elisse Nogarotto, Alexander M. Piper, et al. "Propylene Glycol and Non-Destructive DNA Extractions Enable Preservation and Isolation of Insect and Hosted Bacterial DNA." Agriculture 11, no. 1 (2021): 77. http://dx.doi.org/10.3390/agriculture11010077.

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Plant bio-protection and biosecurity programs worldwide use trap-based surveillance for the early detection of agricultural pests and pathogens to contain their incursions and spread. This task is reliant on effective preservation in insect traps, which is required to maintain specimen quality for extended periods under variable environmental conditions. Furthermore, with traditional morphological examinations now increasingly paired with modern molecular diagnostic techniques, insect traps are required to preserve both the specimens’ morphology and the DNA of insects and vectored bacterial pa

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Malik, Rizal Awaludin, Nilawati Nilawati, Novarina Irnaning Handayani, et al. "LOW ENERGY BACTERIA PRESERVATION OF EXTREMELY HALOPHILIC ARCHAEA HALOFERAX LUCENTENSE AND HALOFERAX CHUDINOVII IMMOBILIZED USING NATURAL ZEOLITE." Jurnal Riset Teknologi Pencegahan Pencemaran Industri 10, no. 2 (2019): 16–28. http://dx.doi.org/10.21771/jrtppi.2019.v10.no2.p16-28.

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The methods of microbial cells preservation were already known by liquid drying, freeze-drying, and freezing. Those methods could preserve bacteria cells in a long period of time but its survivability was relatively low and used relatively high energy during preservation. Immobilization was known as entrapping, attaching or encapsulating bacterial cells in a suitable matrix. This research was conducted to know the suitability of zeolite as immobilization carrier and also as preservation matrix of two halophilic archaea Haloferax chudinovii and Haloferax lucentense. Variable of this research wa

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Watve, M. G. "Preservation of bacterial colony morphology." MIRCEN Journal of Applied Microbiology and Biotechnology 4, no. 4 (1988): 495–96. http://dx.doi.org/10.1007/bf00940177.

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Miao, Xuelan, Yanhua Zhao, Lingxi Zhu, et al. "The Equilibrium of Bacterial Microecosystem: Probiotics, Pathogenic Bacteria, and Natural Antimicrobial Substances in Semen." Microorganisms 12, no. 11 (2024): 2253. http://dx.doi.org/10.3390/microorganisms12112253.

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Semen is a complex fluid that contains spermatozoa and also functions as a dynamic bacterial microecosystem, comprising probiotics, pathogenic bacteria, and natural antimicrobial substances. Probiotic bacteria, such as Lactobacillus and Bifidobacterium, along with pathogenic bacteria like Pseudomonas aeruginosa and Escherichia coli, play significant roles in semen preservation and reproductive health. Studies have explored the impact of pathogenic bacteria on sperm quality, providing insights into the bacterial populations in mammalian semen and their influence on sperm function. These reviews

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Sáenz, Johan S., Bibiana Rios-Galicia, Bianca Rehkugler, and Jana Seifert. "Dynamic Development of Viral and Bacterial Diversity during Grass Silage Preservation." Viruses 15, no. 4 (2023): 951. http://dx.doi.org/10.3390/v15040951.

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Ensilaging is one of the most common feed preservation processes using lactic acid bacteria to stabilize feed and save feed quality. The silage bacterial community is well known but the role of the virome and its relationship with the bacterial community is scarce. In the present study, metagenomics and amplicon sequencing were used to describe the composition of the bacterial and viral community during a 40-day grass silage preservation. During the first two days, we observed a rapid decrease in the pH and a shift in the bacterial and viral composition. The diversity of the dominant virus ope

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Wang, Xiuling, Shunjie Liu, Mingjie Chen, et al. "Low Temperature (15 °C) Reduces Bacterial Diversity and Prolongs the Preservation Time of Volvariella volvacea." Microorganisms 7, no. 10 (2019): 475. http://dx.doi.org/10.3390/microorganisms7100475.

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Straw mushroom (Volvariella volvacea) is the most commonly cultivated edible fungus in the world, but the challenges associated with the preservation have limited its marketability. Microbiology, especially bacteria, play a key role in the deterioration of food, this study aimed to reveal the succession of the bacterial community on the surfaces of V. volvacea fruit bodies under different temperature conditions. We amplified 16S rRNA genes of V4 regions, obtained the bacterial species information by using high-throughput sequencing technology, and analyzed the effects of environmental temperat

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Aldrich, H. C., L. McDowell, A. Choate, and P. Bono. "Preservation and microscopy of bacterial biofilms causing corrosion on steel surfaces." Proceedings, annual meeting, Electron Microscopy Society of America 48, no. 3 (1990): 842–43. http://dx.doi.org/10.1017/s0424820100161771.

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Steel methane transmission lines are sometimes attacked by anaerobic bacteria which cause deep corrosion pits and represent a serious economic problem. We have developed a model laboratory system to follow the process of bacterial attachment and colonization of test specimens of pipeline steel. Test coupons of X and B grades of steel approximately 5mm x 10mm x 20mm were exposed to combinations of the bacteria Enterobacter aerogenes, Desulfovibrio desulfuricans, and Clostridium acetobutylicum in an aerobic culture for periods ranging from 2 hr. to 30 days.A method is described for preserving ba

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Ashikhmina, Mariia S., Vladislav S. Filozop, Mikhail O. Volodarskiy, Sviatlana A. Ulasevich, and Ekaterina V. Skorb. "OPTIMIZATION OF BACTERIAL LYOPHILIZATION PROCESS: INFLUENCE OF CULTIVATION AND LYOPHILIZATION PARAMETERS ON PROBIOTIC SURVIVAL RATE." Siberian Journal of Life Sciences and Agriculture 16, no. 6 (2024): 515–34. https://doi.org/10.12731/2658-6649-2024-16-6-1008.

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Background. The preservation of bacterial cultures plays an important role in scientific research and industrial production, both food and medical. The study of the influence of lyophilization process on bacterial survival is important for the development of effective methods of probiotic application. Based on the analysis of more than three hundred publications by domestic and foreign research groups, about thirty key factors influencing the survival of bacteria during the lyophilization process have been identified. However, for the equation describing the survival rate of bacteria during ly

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Sariasih, Santi, Fitri Widiantini, and Wiwin Widiawati. "Metode Penyimpanan Bakteri Xanthomonas Oryzae pv. oryzae Penyebab Penyakit Hawar Daun Bakteri Pada Tanaman Padi Menggunakan Glycerol." Jurnal Pengelolaan Laboratorium Pendidikan 2, no. 1 (2020): 1–7. http://dx.doi.org/10.14710/jplp.2.1.1-7.

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The existence of Xanthomonas oryzae pv. oryzae, the bacteria which causes Bacterial Leaf Blight of rice, is really necessary for the laboratory to support the research activities of the phytopathology experts. Long-term preservation by using glycerol under -20°C can be conducted to preserve the culture’s availability to stay viable in the laboratory. This research aimed to determine the glycerol concentration that is able to preserve the viability and pathogenicity of Xanthomonas oryzae pv. oryzae bacteria after being preserved for 3 and 6 months. This experiments were performed using Complete

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Ďuračka, Michal, Filip Benko, Milan Chňapek, and Eva Tvrdá. "Strategies for Bacterial Eradication from Human and Animal Semen Samples: Current Options and Future Alternatives." Sensors 23, no. 15 (2023): 6978. http://dx.doi.org/10.3390/s23156978.

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The primary role of semen processing and preservation is to maintain a high proportion of structurally and functionally competent and mature spermatozoa, that may be used for the purposes of artificial reproduction when needed, whilst minimizing any potential causes of sperm deterioration during ex vivo semen handling. Out of a multitude of variables determining the success of sperm preservation, bacterial contamination has been acknowledged with an increased interest because of its often unpredictable and complex effects on semen quality. Whilst antibiotics are usually the most straight-forwa

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Shen, Hongyi. "Bacillus Species’ Gaseous Metabolites Prolong the Shelf Life of Apples and Grapes." International Journal of Natural Resources and Environmental Studies 3, no. 2 (2024): 75–81. http://dx.doi.org/10.62051/ijnres.v3n2.11.

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In view of the threat to the environment posed by the widely used plant and fruit preservation chemicals in current agricultural practices, and the indispensability of fruit and plant preservatives in ensuring the yield of agricultural products, it is urgent to explore environmentally friendly and efficient preservative alternatives. Against this backdrop, bacterial preservatives stand out as a potential green solution. In this study, 12 strains of Bacillus derived from different ecological niches were carefully screened to evaluate the inhibitory efficacy of their gaseous metabolites against

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Qiu, Jiying, Junhua Wang, Shuangzhi Zhao, et al. "Preservation Study for Raw Conditioned Mutton During Refrigerated Storage by Food Preservatives." Foods 14, no. 9 (2025): 1579. https://doi.org/10.3390/foods14091579.

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The quality modification of chilled, raw conditioned mutton (RCM) after storage significantly impacts consumer preferences, making shelf-life extension and quality preservation crucial. This study evaluated the effects of sodium diacetate (SDA), sodium dehydroacetate (DHA-S), ε-polylysine hydrochloride (PLH), and nisin on RCM quality and bacterial community at concentrations of 3.0, 0.50, 0.30, and 0.50 g/kg, respectively. Major spoilage bacteria were isolated, and the inhibitory effects of these preservatives were studied, leading to the development of compound preservatives. TVC increased si

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WESCHE, ALISSA M., JOSHUA B. GURTLER, BRADLEY P. MARKS, and ELLIOT T. RYSER. "Stress, Sublethal Injury, Resuscitation, and Virulence of Bacterial Foodborne Pathogens†." Journal of Food Protection 72, no. 5 (2009): 1121–38. http://dx.doi.org/10.4315/0362-028x-72.5.1121.

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Environmental stress and food preservation methods (e.g., heating, chilling, acidity, and alkalinity) are known to induce adaptive responses within the bacterial cell. Microorganisms that survive a given stress often gain resistance to that stress or other stresses via cross-protection. The physiological state of a bacterium is an important consideration when studying its response to food preservation techniques. This article reviews the various definitions of injury and stress, sublethal injury of bacteria, stresses that cause this injury, stress adaptation, cellular repair and response mecha

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Rotz, C. A., R. J. Davis, D. R. Buckmaster, and J. W. Thomas. "Bacterial Inoculants for Preservation of Alfalfa Hay." Journal of Production Agriculture 1, no. 4 (1988): 362–67. http://dx.doi.org/10.2134/jpa1988.0362.

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Brundin, Malin, David Figdor, Anders Johansson, and Ulf Sjögren. "Preservation of Bacterial DNA by Human Dentin." Journal of Endodontics 40, no. 2 (2014): 241–45. http://dx.doi.org/10.1016/j.joen.2013.08.025.

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Daman, Preet Kour* Neeraj Gupta Seerat Gupta and Jesreen Chauhan. "Bacteriocins and its application in food preservation." Science World a monthly e magazine 3, no. 10 (2023): 2511–14. https://doi.org/10.5281/zenodo.8418022.

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Biopreservation is a method of food preservation that employs natural antimicrobials (biopreservatives) and microbiota to extend food storage life. Consumers are increasingly seeking chemical-free foods due to their reported negative effects. Bacteriocins are bactericidal proteins or peptides that can inhibit the development of food- spoiling bacteria. These substances are made by various gram-positive and gram-negative microorganisms, each of which has its own molecular weight, biochemical makeup, and mode of operation. The majority of bacteriocins target an individual bacterial strain or spe

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Ziklo, Noa, Inbal Tzafrir, Regina Shulkin, and Paul Salama. "Salicylate UV-Filters in Sunscreen Formulations Compromise the Preservative System Efficacy against Pseudomonas aeruginosa and Burkholderia cepacia." Cosmetics 7, no. 3 (2020): 63. http://dx.doi.org/10.3390/cosmetics7030063.

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Contamination of personal-care products are a serious health concern and therefore, preservative solutions are necessary for the costumers’ safety. High sun protection factor (SPF) sunscreen formulations are known to be difficult to preserve, due to their high ratio of organic phase containing the UV-filters. Salicylate esters such as octyl salicylate (OS) and homosalate (HS) are among the most common UV-filters currently used in the market, and can undergo hydrolysis by esterase molecules produced by contaminant microorganisms. The hydrolysis product, salicylic acid (SA) can be assimilated by

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Yadav, Ajay, Abhijeet Singh, Adarsh Singh, Anupam Yadav, and Sashank Singh. "Segmentation of Microscopy images using Multi-Scale Retinex with Chromacity Preservation and Otsu Thresholding." March 2023 5, no. 1 (2023): 12–23. http://dx.doi.org/10.36548/jaicn.2023.1.002.

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Bacteria play a significant role in our environment by being helpful or harmful; hence, it is crucial to identify the various bacterial species. The microscopic image captured by camera with microscope is not reliable due to the poor quality of image, making bacterial counting a difficult and time-consuming task. This paper proposes improved and enhanced Multi-Scale Retinex with Chromacity Preservation and Otsu Thresholding techniques for increasing the quality of images of bacterial cells for segmentation and contrast enhancement. A combinative procedure of image enhancement and segmentation

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Yadav, Ajay, Abhijeet Singh, Adarsh Singh, Anupam Yadav, and Sashank Singh. "Segmentation of Microscopy images using Multi-Scale Retinex with Chromacity Preservation and Otsu Thresholding." March 2023 5, no. 1 (2023): 12–23. http://dx.doi.org/10.36548//jaicn.2023.1.002.

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Bacteria play a significant role in our environment by being helpful or harmful; hence, it is crucial to identify the various bacterial species. The microscopic image captured by camera with microscope is not reliable due to the poor quality of image, making bacterial counting a difficult and time-consuming task. This paper proposes improved and enhanced Multi-Scale Retinex with Chromacity Preservation and Otsu Thresholding techniques for increasing the quality of images of bacterial cells for segmentation and contrast enhancement. A combinative procedure of image enhancement and segmentation

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Qiu, Qinghua, Tanghui Long, Kehan Ouyang, et al. "Effect of Preservation Temperature and Time on Fermentation Characteristics, Bacterial Diversity and Community Composition of Rumen Fluid Collected from High-Grain Feeding Sheep." Fermentation 9, no. 5 (2023): 466. http://dx.doi.org/10.3390/fermentation9050466.

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This study aimed to explore the dynamic variations in fermentation characteristics, bacterial diversity and community composition at two preservation temperatures as preservation time extended. Six rumen fluid samples collected from high-grain feeding sheep were stored at −20 °C or −80 °C for 0 day, 7 days, 14 days, 30 days, 60 days, 120 days, and 240 days. The results showed that the current preservation temperature did not alter the fermentation characteristics, bacterial diversity and community composition (p > 0.05). The concentrations of ammonia, microbial crude protein, acetate, propi

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Zuo, Hong, Bo Wang, Jiamin Zhang, Zhengguo Zhong, and Zhonghua Tang. "Research Progress on Bacteria-Reducing Pretreatment Technology of Meat." Foods 13, no. 15 (2024): 2361. http://dx.doi.org/10.3390/foods13152361.

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Reducing the initial bacteria number from meat and extending its shelf life are crucial factors for ensuring product safety and enhancing economic benefits for enterprises. Currently, controlling enzyme activity and the microbial survival environment is a common approach to reducing the rate of deterioration in raw meat materials, thereby achieving the goal of bacteria reduction during storage and preservation. This review summarizes the commonly used technologies for reducing bacteria in meat, including slightly acidic electrolyzed water (SAEW), organic acids, ozone (O3), ultrasound, irradiat

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Aman Ullah, Aman Ullah. "Causes of Meat Deterioration and Preservation Methods." Indian Journal of Pure & Applied Biosciences 11, no. 1 (2023): 14–22. http://dx.doi.org/10.18782/2582-2845.8970.

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Humans have been practising the skill of meat preservation since the beginning of time, and this practice lives on in all of the foods we consume today. Primarily, meat preservation postpones the development of microorganisms like bacteria and fungi, so meat and its byproducts may sit on store shelves for longer without losing quality or flavour. Dysbiosis is the process by which any food, when exposed to organisms (bacterial and fungal), that degrade the products, changes from being edible to being inedible via denaturation of texture, color, and odor. Spoilage and a lack of storage skills du

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Montero Castillo, Piedad M., Antonio Díaz Caballero, and Marlene Durán Lengua. "Antagonistic action of Lactobacillus spp. against Staphylococcus aureus in cheese from Mompox - Colombia." Revista Facultad Nacional de Agronomía Medellín 68, no. 2 (2015): 7721–27. http://dx.doi.org/10.15446/rfnam.v68n2.50991.

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In the food industry, food preservation techniques that do not use chemical products are becoming more common. Therefore, the aim of this research was to evaluate the antagonistic activity (antibiosis) of lactic-acid bacterial strains against pathogenic microorganisms. Lactic-acid bacterial strains were isolated from layered cheese and a commercial product (yogurt); and the same was done with pathogenic bacteria solely from layered cheese. The lactic-acid bacterial strains were identified as species from the Lactobacilli family, while the pathogenic bacteria from layered cheese were identified

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Schultz, SG. "Volume Preservation: Then and Now." Physiology 4, no. 5 (1989): 169–72. http://dx.doi.org/10.1152/physiologyonline.1989.4.5.169.

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Volume regulatory mechanisms, which evolved in Precambrian life and served to prevent cell lysis when our bacterial and invertebrate ancestors were confronted with hypotonic extracellular milieus, have been preserved in vertebrate cells but with redefined and highly sophisticated physiological functions.

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Fedak, N., S. Chumachenko, L. Darmohray, B. Gutyj, and M. H. Perederiy. "The effectiveness of the use of probiotics for wet maize grain preserving." Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies 20, no. 89 (2018): 85–88. http://dx.doi.org/10.32718/nvlvet8916.

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The process of processing and storage of high moisture grain is one of the important problems of forage production, especially in the Carpathian region due to its excessive moisture, therefore, due to the intensification of the livestock industry, the conservation of feed is becoming increasingly important. The purpose of the research was to determine the effect of different doses of bacterial preparation KT-L 18/1 (produced by Chernihiv ISMAV NAAS) on the preservation of nutrients and microbiological parameters of maize grain of high humidity in dynamics. The investigation of influence differ

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Mahmmoud, Ebtehal N. "Comparison of Preservation Methods of Staphylococcus aureus and Escherichia coli Bacteria." Journal of Pure and Applied Microbiology 14, no. 3 (2020): 2173–80. http://dx.doi.org/10.22207/jpam.14.3.58.

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One of the most important problems faced by microbiologists is to preserve bacterial isolates in the best state to study and further diagnosis. The current study aims to provide a summary of experimental results to maintain two species of bacteria alive after being stored by using some additives. This study found that the best temperature to preserve Staphylococcus aureus was -20°C for a year, while for Escherichia coli it was the same temperature except in using Glycerol (G) 100% and Food oil (FO) methods. The optimum method to preserve S. aureus was by using Normal Saline (NS), while Distill

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Horng, Katti R., Holly H. Ganz, Jonathan A. Eisen, and Stanley L. Marks. "Effects of preservation method on canine (Canis lupus familiaris) fecal microbiota." PeerJ 6 (May 23, 2018): e4827. http://dx.doi.org/10.7717/peerj.4827.

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Studies involving gut microbiome analysis play an increasing role in the evaluation of health and disease in humans and animals alike. Fecal sampling methods for DNA preservation in laboratory, clinical, and field settings can greatly influence inferences of microbial composition and diversity, but are often inconsistent and under-investigated between studies. Many laboratories have utilized either temperature control or preservation buffers for optimization of DNA preservation, but few studies have evaluated the effects of combining both methods to preserve fecal microbiota. To determine the

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Bahuguna, Shivohum, Magda Atilano, Marcus Glittenberg, et al. "Bacterial recognition by PGRP-SA and downstream signalling by Toll/DIF sustain commensal gut bacteria in Drosophila." PLOS Genetics 18, no. 1 (2022): e1009992. http://dx.doi.org/10.1371/journal.pgen.1009992.

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The gut sets the immune and metabolic parameters for the survival of commensal bacteria. We report that in Drosophila, deficiency in bacterial recognition upstream of Toll/NF-κB signalling resulted in reduced density and diversity of gut bacteria. Translational regulation factor 4E-BP, a transcriptional target of Toll/NF-κB, mediated this host-bacteriome interaction. In healthy flies, Toll activated 4E-BP, which enabled fat catabolism, which resulted in sustaining of the bacteriome. The presence of gut bacteria kept Toll signalling activity thus ensuring the feedback loop of their own preserva

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A. Shaltout, Fahim. "Impacts of meat spoilage on economy and public health." Nutrition and Food Processing 7, no. 6 (2024): 01–06. http://dx.doi.org/10.31579/2637-8914/217.

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The meat spoilage cause shortening of shelf life of the meat, So it has negative effect on economy on economy. The meat spoilage occurred due to microbial growth so the meat spoilage has public health hazard. The meat preservation started with mankind from day one of living and is still carried in every type of meat such as beef, mutton fish, poultry and their products. The basic concept of the meat preservation is to increase the shelf life of the meat and meat product without deteriorating the quality and texture of the meat products by delaying the growth of different types of bacterial and

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DU, HECHAO, XIUXIU LI, ZHAOXIN LU, et al. "Effect of Plantaricin 163 in Combination with Thymol and Surfactin on Crucian Carp (Carassius auratus)." Journal of Food Protection 82, no. 8 (2019): 1283–91. http://dx.doi.org/10.4315/0362-028x.jfp-18-439.

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ABSTRACT The use of natural preservatives has attracted considerable attention owing to their generally safe and environmentally friendly properties. In this study, we investigated the effects of the preservative A1, composed of plantaricin 163, thymol, and surfactin, on bacterial communities and storage quality of refrigerated crucian carp. A total of 522 operational taxonomic units belonging to 20 phyla and 272 genera were identified by high-throughput sequencing, showing a comprehensive coverage of bacterial composition of crucian carp. In untreated samples after spoilage, Brochothrix was t

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Mukhida, Sahjid, Shazia Chavan, Vishal Pandya, and Jaydev Pandya. "Preparation and validation of skim milk-based method for preservation of bacterial strain at resource-limited setup." Journal of Family Medicine and Primary Care 14, no. 5 (2025): 1833–42. https://doi.org/10.4103/jfmpc.jfmpc_1626_24.

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ABSTRACT Introduction and Objectives: To ensure quality control, microbiology laboratories should maintain a variety of bacterial and fungal strains. Once cultivated, these organisms can survive on the media as long as nutrients are available. Skim milk, a common freeze-drying protectant, can be used as a freezing solution for bacteria at low temperatures. The aim of this study was to prepare and validate a skim milk solution for bacterial strain storage. Methods: A 10% skim milk solution was prepared and stored into 2–3 ml aliquots in sterile plastic vials. Subcultured organisms were inoculat

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Shuster, Jeremiah, Maria Rea, Barbara Etschmann, Joël Brugger, and Frank Reith. "Terraced Iron Formations: Biogeochemical Processes Contributing to Microbial Biomineralization and Microfossil Preservation." Geosciences 8, no. 12 (2018): 480. http://dx.doi.org/10.3390/geosciences8120480.

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Terraced iron formations (TIFs) are laminated structures that cover square meter-size areas on the surface of weathered bench faces and tailings piles at the Mount Morgan mine, which is a non-operational open pit mine located in Queensland, Australia. Sampled TIFs were analyzed using molecular and microanalytical techniques to assess the bacterial communities that likely contributed to the development of these structures. The bacterial community from the TIFs was more diverse compared to the tailings on which the TIFs had formed. The detection of both chemolithotrophic iron-oxidizing bacteria,

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Marushkina, O. I. "Therapy of bacterial vaginosis." Medical Council, no. 7 (April 5, 2019): 104–9. http://dx.doi.org/10.21518/2079-701x-2019-7-104-109.

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Aim:evaluation of efficacy of Multi-Gyn® Actigel application in the complex therapy of bacterial vaginosis (BV) and prevention of its recurrence in pregnant women.Patients and methods:87 patients of reproductive age were examined and treated with the diagnosis of «bacterial vaginosis, chronic recurrent course». 41 of them from the main group were given Multi-Gyn® Actigel at the 1st stage of treatment, while 46 women of the comparison group were given 2% clindamycin cream In order to restore vaginal microbiocenosis, a probiotic containing Lactobacillus casei rhamnosus spp. LCR35 was used in bot

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Pylak, Michał, Karolina Oszust, and Magdalena Frąc. "Optimization of Growing Medium and Preservation Methods for Plant Beneficial Bacteria, and Formulating a Microbial Biopreparation for Raspberry Naturalization." Agronomy 11, no. 12 (2021): 2521. http://dx.doi.org/10.3390/agronomy11122521.

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The current study focuses on the optimization of bacterial growing medium composition, including the carbon and nitrogen source in different concentrations, the pH value of the medium and the temperature. Optimization was performed for four environmental bacterial isolates belonging to the genera Arthrobacter, Pseudomonas and Rhodococcus, which were previously obtained from wild raspberries. These bacteria proved to be potent antagonists against certain fungal and fungal-like plant pathogens. Furthermore, three preservation methods and three sample preparation techniques were evaluated. In add

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HAU, LUNG-BIN, MING-HSIN LIEW, and LEA-TE YEH. "Preservation of Grass Prawns by Ionizing Radiation." Journal of Food Protection 55, no. 3 (1992): 198–202. http://dx.doi.org/10.4315/0362-028x-55.3.198.

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The purpose of this study was to evaluate the feasibility of preserving grass prawns (Penaeus monodon) by ionizing radiation. Frozen prawns were irradiated with different doses at −10 ± 2°C. The threshold dose for detecting irradiation flavor was 4.5 kGy. The D10 values for Vibrio cholera, Staphylococcus aureus, Escherichia coli, and Salmonella enteritidis were 0.11, 0.29, 0.39, and 0.48 kGy, respectively. Irradiation with doses of 2.5, 5.0, and 7.5 kGy reduced the total bacterial counts from 105 CFU/g to 4.0 × 103, 1.6 × 103, and 3.2 × 102 CFU/g, respectively. The bacterial counts of the irra

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Webster, P., S. Wu, S. Webster, K. A. Rich, and K. McDonald. "Ultrastructural preservation of biofilms formed by non-typeable Hemophilus influenzae." Biofilms 1, no. 3 (2004): 165–82. http://dx.doi.org/10.1017/s1479050504001425.

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There is growing evidence to suggest that non-typeable Hemophilus influenzae (NTHi), an important cause of otitis media in children, is able to grow as a biofilm in the middle ear. This observation may help to explain bacterial persistence in chronic infections. We evaluate the usefulness of rapid freezing and freeze substitution as a means of preparing biofilms for ultrastructural examination by comparing the morphology of cryofixed specimens with the morphology produced using more conventional chemical fixation and dehydration methods. Chemical fixation and dehydration methods produced subst

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Adebowale, Modupe, Michael Adewumi, and B. C. Makanjuola. "Analysis of Thermophilic and Halophilic Bacteria in Traditonally Processed Locust Beans Sold in Ekiti and Kwara State, Nigeria." International Journal of Research and Scientific Innovation XI, no. IV (2024): 956–67. http://dx.doi.org/10.51244/ijrsi.2024.1104068.

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Locust beans is one of the most essential condiment and ingredient of any soup in Nigeria. Many household and eateries make use of this condiment on daily basis, considering its taste and addition to every soup, coupled with its health benefit. Being a natural ingredient, some medical practitioner had successfully convince their patient to abstain from many other artificial soup ingredient and stick to locust beans for their food. The Process and preservation of this locust beans involves cooking, drying and salting, which ordinarily should have killed every bacterial in the process, though, t

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Barnes, Ian, and Mark G. Thomas. "Evaluating bacterial pathogen DNA preservation in museum osteological collections." Proceedings of the Royal Society B: Biological Sciences 273, no. 1587 (2005): 645–53. http://dx.doi.org/10.1098/rspb.2005.3339.

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Harry, Myriam, Béatrice Gambier, and Evelyne Garnier-Sillam. "Soil conservation for DNA preservation for bacterial molecular studies." European Journal of Soil Biology 36, no. 1 (2000): 51–55. http://dx.doi.org/10.1016/s1164-5563(00)00044-3.

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Vandevivere, P., and P. Baveye. "Improved preservation of bacterial exopolymers for scanning electron microscopy." Journal of Microscopy 167, no. 3 (1992): 323–30. http://dx.doi.org/10.1111/j.1365-2818.1992.tb03242.x.

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Hassan, Farazia, Sehar Anwar, Mukesh Mukesh, et al. "The synergistic effect of non-thermal techniques and modified atmosphere packaging in food preservation." International Journal of Environment, Agriculture and Biotechnology 8, no. 6 (2023): 246–59. http://dx.doi.org/10.22161/ijeab.86.20.

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Consumer’s demand for food products that retain the natural properties and microbiologically safer food has promoted the use of non-thermal techniques for the reduction of microbial load and inactivation of enzymatic activity. However, the bacterial spores and some enzymes show high resistance against non-thermal techniques. Therefore, the application of non-thermal techniques with modified atmosphere packaging (MAP) represents an emerging method to increase the shelf life of food products. These combined preservation techniques reduced the microbial load, increase the shelf life without effec

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Souza, Luana Virgínia, Evandro Martins, Isabella Maria Fernandes Botelho Moreira, and Antônio Fernandes de Carvalho. "Strategies for the Development of Bioprotective Cultures in Food Preservation." International Journal of Microbiology 2022 (December 14, 2022): 1–16. http://dx.doi.org/10.1155/2022/6264170.

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Consumers worldwide are increasingly demanding food with fewer ingredients, preferably without chemical additives. The trend called “Clean Label” has stimulated the development and commercialization of new types of bioprotective bacterial cultures. These bacteria are not considered new, and several cultures have been available on the market. Additionally, new bioprotective bacteria are being identified to service the clean label trend, extend the shelf life, and, mainly, improve the food safety of food. In this context, the lactic acid bacteria (LAB) have been extensively prospected as a biopr

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Gao, Feng, Qiyu Liu, Peipei Wang, and Yongge Liu. "Reinforcement of Oracle Bones Using a Novel Silicone Coupling Reagent for Preservation." Coatings 14, no. 11 (2024): 1430. http://dx.doi.org/10.3390/coatings14111430.

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Oracle bones are artifacts of great significance and value in the study of Chinese history and culture. Because of soil and bacterial erosion, bones become fragile, and the inscriptions on the surface become blurred, resulting in the loss of historical information on the bones. In early times, scholars often used adhesives for bonding and reinforcement, whereas in modern times, organic and inorganic materials have been used as reinforcement for oracle bones. In this study, the surface of oracle bone was protected and reinforced by a new silicone coupling reagent that could self-polymerize in t

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Dorjnamjaa, Dorj, Gundsambuu Altanshagai, and Batkhuyag Enkhbaatar. "New scientific direction of the bacterial paleontology in Mongolia." Mongolian Geoscientist, no. 49 (October 13, 2019): 41–49. http://dx.doi.org/10.5564/mgs.v0i49.1226.

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We review the initial development of Bacterial Paleontology in Mongolia and present some electron microscopic images of fossil bacteria in different stages of preservation in sedimentary rocks. Indeed bacterial paleontology is one the youngest branches of paleontology. It has began in the end of 20th century and has developed rapidly in recent years. The main tasks of bacterial paleontology are detailed investigation of fossil microorganisms, in particular their morphology and sizes, conditions of burial and products of habitation that are reflected in lithological and geochemical features of

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Aditya, Dwi Martha Nur, Adhimas Setyo Wicaksono, Acik Afandi, Lady Theresa Adeodata, and Lisa Thalia Mulyanata. "NEUTRALIZE BACTERIAL ACTIVITY WITH ANATOMICAL EMBALMING SOLUTIONS." Jurnal Riset Kesehatan 11, no. 2 (2022): 123–30. http://dx.doi.org/10.31983/jrk.v11i2.9234.

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Formaldehyde had some degree of toxicity in the human body as a cadaver preservation solution. Recent studies showed that moderate formaldehyde levels in cadavers could neutralize SARS-CoV-2. However, other effects of formaldehyde levels in the air are not yet known on bacteria. This study aims to determine the optimal level of formaldehyde that can be used to neutralize bacteria and is safe for humans. This study used a post-test control group design with formaldehyde level as the independent variable and bacterial colonization as the dependent variable. The results showed that the mean level

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Rojas-Tapias, Daniel, Oriana Ortega Sierra, Diego Rivera Botía, and Ruth Bonilla. "Preservation of Azotobacter chroococcum vegetative cells in dry polymers." Universitas Scientiarum 20, no. 2 (2014): 201. http://dx.doi.org/10.11144/javeriana.sc20-2.pacv.

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We studied the preservation of Azotobacter chroococcum C26 using three dry polymers: carrageenin, sodium alginate, and HPMC, using a method of accelerated degradation. Bacterial viability, as response variable, was measured at three temperatures in four different times, which was followed by calculation of bacterial degradation rates. Results showed that temperature, time of storage, and protective agent influenced both viability and degradation rates (P;lt;0.05). We observed, using the Arrhenius thermodynamic model, that the use of polymers increased the activation energy of bacterial degrada

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Manpoong, Nang Kanna, Kutubuddin Ahmed, Dilip Kumar Bhattacharya, Dipak Kumar Sarma, Nekibuddin Ahmed, and Arunoday Das. "Efficacy of potassium permanganate and turmeric as antimicrobial agents on the bacterial load and quality of boar semen during preservation at 15 °C." Veterinarski arhiv 90, no. 5 (2020): 443–51. http://dx.doi.org/10.24099/vet.arhiv.0715.

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The present study was conducted to evaluate the efficacy of different natural antimicrobials agents (KMnO4 and Turmeric) in comparison with conventional antibiotics against the bacterial load and in relation to the quality of boar semen in Modena extender for up to 120 hours of preservation at 15 °C. A total of 56 ejaculates, 14 from each of four Hampshire crossbred boars maintained within the ICAR-AICRP on Pigs, in Guwahati, Assam, India, were utilized in the study. Thirty-two ejaculates, 8 from each of four boars were used to study the effect of antimicrobial agents on semen quality during p

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Bembeeva, B. O., E. L. Isaeva, V. V. Muravieva, et al. "Optimising Conditions for the Preservation of Extremely Oxygen Sensitive Obligate Anaerobes of the Gut Microbiota as Candidates for Probiotic Strains." Epidemiology and Vaccinal Prevention 23, no. 6 (2025): 54–60. https://doi.org/10.31631/2073-3046-2024-23-6-54-60.

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Obligate anaerobic difficult culture bacteria, which make up the bulk of the microbiota of the large intestine, are potential candidates for the development of highly effective new generation probiotics, since they are capable of synthesizing a variety of metabolites, including short-chain fatty acids, which have a stimulating effect on both commensal bacteria and host cells. However, for their long-term preservation, it is necessary to carefully select the preservation method and protective components. To evaluate the efficacy of using different cryoprotectants to increase the viability of ob

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Antibus, Doug E., Laura G. Leff, Brenda L. Hall, Jenny L. Baeseman, and Christopher B. Blackwood. "Molecular characterization of ancient algal mats from the McMurdo Dry Valleys, Antarctica." Antarctic Science 24, no. 2 (2011): 139–46. http://dx.doi.org/10.1017/s0954102011000770.

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AbstractThe McMurdo Dry Valleys possess a cold and dry climate which favours biomolecular preservation, and present the possibility for preservation of biological materials over long timescales. We examined patterns of bacterial DNA abundance and diversity in algal mats from 8–26 539 years of age. Bacterial DNA abundance was inferred from extractable DNA quantity and quantitative polymerase chain reaction targeting the bacterial 16S rRNA gene. Because damage to bacterial DNA could limit its availability for polymerase chain reaction, the efficacy of DNA repair by a commercially available kit w

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