Academic literature on the topic 'Cell culture'

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Journal articles on the topic "Cell culture"

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Ylostalo, Joni H. "3D Stem Cell Culture." Cells 9, no. 10 (2020): 2178. http://dx.doi.org/10.3390/cells9102178.

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Much interest has been directed towards stem cells, both in basic and translational research, to understand basic stem cell biology and to develop new therapies for many disorders. In general, stem cells can be cultured with relative ease, however, most common culture methods for stem cells employ 2D techniques using plastic. These cultures do not well represent the stem cell niches in the body, which are delicate microenvironments composed of not only stem cells, but also supporting stromal cells, extracellular matrix, and growth factors. Therefore, researchers and clinicians have been seekin
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Lindee, M. S. "CELL BIOLOGY: The Culture of Cell Culture." Science 316, no. 5831 (2007): 1568–69. http://dx.doi.org/10.1126/science.1142574.

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Bauer, Magdalena, Magdalena Metzger, Marvin Corea, Barbara Schädl, Johannes Grillari, and Peter Dungel. "Novel 3D-Printed Cell Culture Inserts for Air–Liquid Interface Cell Culture." Life 12, no. 8 (2022): 1216. http://dx.doi.org/10.3390/life12081216.

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In skin research, widely used in vitro 2D monolayer models do not sufficiently mimic physiological properties. To replace, reduce, and refine animal experimentation in the spirit of ‘3Rs’, new approaches such as 3D skin equivalents (SE) are needed to close the in vitro/in vivo gap. Cell culture inserts to culture SE are commercially available, however, these inserts are expensive and of limited versatility regarding experimental settings. This study aimed to design novel cell culture inserts fabricated on commercially available 3D printers for the generation of full-thickness SE. A computer-ai
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First, NL, MM Sims, SP Park, and MJ Kent-First. "Systems for production of calves from cultured bovine embryonic cells." Reproduction, Fertility and Development 6, no. 5 (1994): 553. http://dx.doi.org/10.1071/rd9940553.

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The development of totipotent bovine embryonic cell cultures has great value in cattle breeding. They provide: (1) a mechanism for making large numbers of clonal offspring by nuclear transfer; (2) an efficient gene transfer system through the use of selectable markers to select transgenic cells; and (3) a mechanism for site-specific gene transfer or deletion by homologous DNA sequence recombination. Bovine embryonic cell cultures have been established from blastocyst inner cell mass (ICM) cells, morulae and the precompaction 16-20-cell stage. All have exhibited similar morphology to mouse embr
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Finoli, Anthony, Eva Schmelzer, Patrick Over, Ian Nettleship, and Joerg C. Gerlach. "Open-Porous Hydroxyapatite Scaffolds for Three-Dimensional Culture of Human Adult Liver Cells." BioMed Research International 2016 (2016): 1–7. http://dx.doi.org/10.1155/2016/6040146.

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Liver cell culture within three-dimensional structures provides an improved culture system for various applications in basic research, pharmacological screening, and implantable or extracorporeal liver support. Biodegradable calcium-based scaffolds in such systems could enhance liver cell functionality by providing endothelial and hepatic cell support through locally elevated calcium levels, increased surface area for cell attachment, and allowing three-dimensional tissue restructuring. Open-porous hydroxyapatite scaffolds were fabricated and seeded with primary adult human liver cells, which
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Levine, J. F., and F. E. Stockdale. "Cell-cell interactions promote mammary epithelial cell differentiation." Journal of Cell Biology 100, no. 5 (1985): 1415–22. http://dx.doi.org/10.1083/jcb.100.5.1415.

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Mammary epithelium differentiates in a stromal milieu of adipocytes and fibroblasts. To investigate cell-cell interactions that may influence mammary epithelial cell differentiation, we developed a co-culture system of murine mammary epithelium and adipocytes and other fibroblasts. Insofar as caseins are specific molecular markers of mammary epithelial differentiation, rat anti-mouse casein monoclonal antibodies were raised against the three major mouse casein components to study this interaction. Mammary epithelium from mid-pregnant mice was plated on confluent irradiated monolayers of 3T3-L1
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Kakigi, Akinobu. "Cell Culture." Equilibrium Research 67, no. 1 (2008): 1–5. http://dx.doi.org/10.3757/jser.67.1.

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Ponec, Maria, and Esther Boelsma. "Cell Culture." American Journal of Contact Dermatitis 8, no. 2 (1997): 100–102. http://dx.doi.org/10.1097/01634989-199706000-00021.

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Ponec, Maria, and Esther Boelsma. "Cell Culture." Dermatitis 8, no. 2 (1997): 100–102. http://dx.doi.org/10.1097/01206501-199706000-00021.

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DeGaspari, John. "Cell Culture." Mechanical Engineering 123, no. 03 (2001): 56–59. http://dx.doi.org/10.1115/1.2001-mar-1.

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This article focuses on Tribon Bearing Co. plant in Brook Park, OH, a manufacturer of discrete carbon composite parts and shapes that had been plagued by problems that threatened its existence. The old Tribon plant was a traditional manufacturing setup, in which operations were highly compartmentalized. Equipment was arranged according to purpose and job functions were narrowly defined. The plant’s production control manager, there was plenty of distrust and bad feelings between front-line management and the plant floor workforce. Workcell leaders work with manufacturing engineers to develop a
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Dissertations / Theses on the topic "Cell culture"

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Liu, Mengfei, and 刘梦菲. "Epithelial morphogenesis in three-dimensional cell culture system." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2014. http://hdl.handle.net/10722/208611.

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In human body, the most common structures formed by epithelial cells are hollow cysts or tubules. The key feature of the cysts and tubules is the central lumen, which is lined by epithelial cell sheets. The central lumen allows material exchange, thus it is indispensable for the proper function of the epithelial tissue. In order to understand the way that the epithelial cells form highly specialized structure, an in vitro three-dimensional (3D) culture system was established. The Caco-2 cells were embedded in reconstituted basement membrane termed matrigel, whose biochemical constitution and
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Theil, Ian. "Anchorage-dependent mammalian cell culture." Thesis, McGill University, 1992. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=56768.

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Genetically engineered anchorage-dependent human embryonic kidney (293) cells were cultured at 37$ sp circ$C on 1 mm thick sheets of a fibrous polymeric matrix having an average fibre diameter of 10.2 $ mu$m and a void fraction of 0.81 using Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 2.5 mM glutamine. Immobilization efficiencies above 70% were observed when cells were added to 100 mL spinner flasks (operating at 60 rpm) containing 70 mL of medium and two 1 x 1 cm squares of matrix (total gross area of 2 cm$ sp2$) fastened to the base of the sti
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Machado, Roque Ana Isabel. "Protein scaffolds for cell culture." Thesis, University of Newcastle Upon Tyne, 2013. http://hdl.handle.net/10443/1843.

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We report here the design, purification and structural characterization of a new protein scaffold for cell culture. Prior studies in our group revealed the structure of the bacterial protein Caf1 to be flexible protein nanofibres, up to 1.5 μm. The existing Cafl expression system was cumbersome and difficult to mutate, we have now produced a system containing the caf operon which allows for the incorporation of specific peptide motifs. The small peptide, RGDS from fibronectin was inserted into 5 different surface loops of Caf1. The Caf1 mutants were expressed and purified and a structural char
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Lee, Kevin Shao-Kwan. "Microscale controlled continuous cell culture." Thesis, Massachusetts Institute of Technology, 2011. http://hdl.handle.net/1721.1/64579.

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Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 2011.<br>Cataloged from PDF version of thesis.<br>Includes bibliographical references (p. 489-500).<br>Measurements of metabolic and cellular activity through substrate and product interactions are highly dependent on environmental conditions and cellular metabolic state. For such experiments to be feasible, continuous cultures are utilized to ensure consistent conditions. However, since medium must be replenished every cell doubling time, costs can be prohibitive in large reactors. An
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Schley, Jeremiah P. "Single Cell Culture Wells (SiCCWells)." The Ohio State University, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=osu1406292709.

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Balagopal, Tulika C., Xiao Lucy Cheng, Jessica Gamboa, John Harper, Sheridan McPheeters, and Bryce Notheis. "A Dynamic Cell Culture System." Thesis, The University of Arizona, 2010. http://hdl.handle.net/10150/146852.

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The following includes information regarding the design, construction, and initial testing of A Dynamic Cell Culture System. This project has been undertaken in order to provide the Wu Lab with a tool that will allow a method to more accurately simulate human vasculature for research purposes by applying pulsatile shear, tensile, and normal pressures. This is accomplished by utilizing upper and lower Chambers between which reside tissue scaffolds (composed of silk and elastin) which are seeded with cells. A high flow-rate pump is used to pass cell culture media over the scaffolds in order to c
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Reiland, Joanne Elizabeth Donovan Maureen D. "Analysis of cell culture models of mammary drug transport." Iowa City : University of Iowa, 2009. http://ir.uiowa.edu/etd/316.

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Ekström, Jens-Ola. "Ljungan Virus Replication in Cell Culture." Doctoral thesis, Högskolan i Kalmar, Naturvetenskapliga institutionen, 2007. http://urn.kb.se/resolve?urn=urn:nbn:se:hik:diva-10.

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Ljungan virus (LV) is a recently identified picornavirus of the genus Parechovirus. LV has been isolated from voles trapped in Sweden and also in the United States. LV infected small rodents may suffer from diabetes type 1 and type 2 like symptoms, myocarditis and encephalitis. LV has been proposed as a human pathogen, with indications of causing diabetes type 1, myocarditis and intrauterine fetal deaths. In this thesis, cell culture adapted LV strains were utilised for development and adaptation of several basic methodological protocols to study the LV biology, e.g. real time PCR, highly spec
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Ekström, Jens-Ola. "Ljungan virus replication in cell culture /." Kalmar : University of Kalmar, 2007. http://urn.kb.se/resolve?urn=urn:nbn:se:hik:diva-10.

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Jayawarna, Vineetha. "Fmoc-peptide gels for cell culture." Thesis, University of Manchester, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.489519.

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Spontaneous formation of macroscopic hydrogels from small molecule building blocks via self-assembly is a powerful tool for the preparation of novel materials with well defined properties. Peptides are particularly interesting as building blocks for these materials. Self-assembled nanowires, fibres, sheets and tubes from peptide systems have all been described.
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Books on the topic "Cell culture"

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Michael, Conn P., ed. Cell culture. Academic Press, 1990.

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Aschner, Michael. Cell culture techniques. Humana Press, 2011.

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M, Loyola-Vargas Victor, and Vázquez-Flota Felipe, eds. Plant cell culture protocols. 2nd ed. Humana Press, 2006.

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1931-, Pinson Arié, ed. The Heart cell in culture. CRC Press, 1987.

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W, Pollard Jeffrey, and Walker John M. 1948-, eds. Animal cell culture. Humana Press, 1990.

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Joanna, Picot, ed. Human cell culture protocols. 2nd ed. Humana Press, 2005.

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J, Jones Christopher, ed. Epithelia: Advances in cell physiology and cell culture. Kluwer Academic Publishers, 1990.

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Pollard, Jeffrey W., and John M. Walker. Animal Cell Culture. Humana Press, 1990. http://dx.doi.org/10.1385/0896031500.

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Langdon, Simon P. Cancer Cell Culture. Humana Press, 2003. http://dx.doi.org/10.1385/1592594069.

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Amini, Shohreh, and Martyn K. White, eds. Neuronal Cell Culture. Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1437-2.

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Book chapters on the topic "Cell culture"

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Buxbaum, Engelbert. "Cell Culture." In Biophysical Chemistry of Proteins. Springer US, 2010. http://dx.doi.org/10.1007/978-1-4419-7251-4_17.

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Nahler, Gerhard. "cell culture." In Dictionary of Pharmaceutical Medicine. Springer Vienna, 2009. http://dx.doi.org/10.1007/978-3-211-89836-9_172.

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Messina, Antonietta, and Loredana De Bartolo. "Cell Culture." In Encyclopedia of Membranes. Springer Berlin Heidelberg, 2016. http://dx.doi.org/10.1007/978-3-662-44324-8_1199.

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Wilson, Anne, and John Graham. "Cell Culture." In Cell Biology Protocols. John Wiley & Sons, Ltd, 2006. http://dx.doi.org/10.1002/0470033487.ch3.

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Gabrys, Beata, John L. Capinera, Jesusa C. Legaspi, et al. "Cell Culture." In Encyclopedia of Entomology. Springer Netherlands, 2008. http://dx.doi.org/10.1007/978-1-4020-6359-6_554.

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Ito, Masaki, and Kiyohiro Houkin. "Cell Culture." In Cell Therapy Against Cerebral Stroke. Springer Japan, 2017. http://dx.doi.org/10.1007/978-4-431-56059-3_5.

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Messina, Antonietta, and Loredana De Bartolo. "Cell Culture." In Encyclopedia of Membranes. Springer Berlin Heidelberg, 2015. http://dx.doi.org/10.1007/978-3-642-40872-4_1199-1.

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Peters, J. H., E. Debus, H. Baumgarten, R. Würzner, M. Schulze, and Helga Gerlach. "Cell Culture." In Monoclonal Antibodies. Springer Berlin Heidelberg, 1992. http://dx.doi.org/10.1007/978-3-642-74532-4_5.

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Gooch, Jan W. "Cell Culture." In Encyclopedic Dictionary of Polymers. Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4419-6247-8_13337.

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Yoon, Jeong-Yeol. "Cell Culture." In Tissue Engineering. Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-83696-2_2.

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Conference papers on the topic "Cell culture"

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Qiu, Q., P. Ducheyne, H. Gao, and P. Ayyaswamy. "Experimental and Numerical Study of Bone Marrow Stromal Cell Culture on Microcarriers in a Rotating-Wall Vessel." In ASME 1997 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 1997. http://dx.doi.org/10.1115/imece1997-1310.

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Abstract The morphology of 3-D rat marrow stromal cell cultures and the expression of bone-related markers were studied in a simulated microgravity environment. In addition, the differences in shear stress on the surface of microcarriers of different densities were calculated. In the experiments, secondary rat marrow stromal cells were cultured on two types of microcarriers, Cytodex-3 beads and modified bioactive glass particles, in a high aspect rotating-wall vessel (HARV). Evaluation of cellular morphology by scanning electron microscopy revealed the presence of 3-D multicellular aggregates
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Ziemczonok, Michal, Sylvia Desissaire, Tigrane Cantat-Moltrecht, and Malgorzata Kujawinska. "Cell and Cell Culture Phantoms for Benchmarking Quantitative Phase Microscopes." In Digital Holography and Three-Dimensional Imaging. Optica Publishing Group, 2024. http://dx.doi.org/10.1364/dh.2024.w4a.39.

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We demonstrate how to design and fabricate artificial cells and cell cultures based on phase images of real specimens. Such cell phantoms enable benchmarking and validation of most quantitative phase imaging instruments in real-world applications.
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Chen, I. Jan, Yu-Yang Lee, and Jay Wang. "Programmable multimodality microscope for automated cell culture monitoring in cell therapy." In Multimodal Biomedical Imaging XX, edited by Xavier Intes, Marien Ochoa, and Mohammad A. Yaseen. SPIE, 2025. https://doi.org/10.1117/12.3043518.

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Bhatia, Sangeeta N., Martin L. Yarmush, and Mehmet Toner. "Engineered Substrates for Controlling Cell-Cell Interactions." In ASME 1997 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 1997. http://dx.doi.org/10.1115/imece1997-1319.

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Abstract Biomaterials have been previously engineered to serve a variety of different functions: precise degradation in vivo (Kimura, 1993), modulation of cell physiology via binding to specific ligands (Hubbell et al, 1992), and selective permeability of certain solutes (Lysaght et al, 1994). However, in complex tissues, where cell-cell interactions strongly influence tissue function, biomaterials which modulate this fundamental parameter have not been available. In this study, we describe a technique which allows control over cell-cell interactions by using semiconductor-based microfabricati
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Isu, Giuseppe, Diana Massai, Giulia Cerino, et al. "A Novel Perfusion Bioreactor for 3D Cell Culture in Microgravity Conditions." In ASME 2013 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2013. http://dx.doi.org/10.1115/sbc2013-14502.

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Cell suspension culture methods based on the generation of microgravity environment are widely used in regenerative medicine for (1) the production of native-like three-dimensional (3D) cell aggregates and engineered tissues [1,2,3], for (2) low cost scalable cell expansion and long-term cell viability maintenance [4,5], and for (3) guiding differentiation of stem cells (SCs) [6]. The generation of a microgravity environment for 3D cell cultures, mimicking the native environment, promotes spatial freedom, cell growth, cell-cell interaction and improves mass transfer and cell exposure to nutrie
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Berthier, R., A. Duperray, O. Valiron, M. Prenant, I. Newton, and A. Schweitzer. "MEGAKARYOCYTIC DEVELOPMENT IN LIQUID CULTURES OF CRYOPRESERVED LEUKOCYTE STEM CELL CONCENTRATES FROM CHRONIC MYELOGENOUS LEUKEMIA PATIENTS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644622.

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The proliferation and differentiation of human megakaryocytes in liquid culture has been obtained using cryopreserved light density blood cell concentrates from chronic myelogenous leukemia (CML) patients. These cryopreserved leukocytes concentrates contain a large number of viable granulo-monocytic, erythroid and megakaryocytic committed stem cells. A high number of spontaneous megakaryocytic colonies was observed in semisolid cultures plated with the CML leukocytes concentrates. A liquid culture system using RPMI 1640 supplemented with 20% human plasma (HP) has been defined where maturing me
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Suganuma, Lisa, Hiromichi Fujie, Hiroki Sudama, et al. "Nanostructure Processed on Culture Plate Improves Cell Adhesion." In ASME 2011 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2011. http://dx.doi.org/10.1115/sbc2011-53753.

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Ligaments and tendons have superior functions, but their healing capacities are limited. We have been developing a novel tissue-engineering technique for the repair of ligaments and tendons which involve stem cell-based self-assembled tissues (scSAT) derived from synovium[1]. For biological reconstruction of soft tissues, it is required for the scSAT to have high tensile strength. Our previous study indicted that, when the scSAT was cultured under high cell density condition, the tensile strength of the scSAT become higher than that cultured under low density condition[2]. However, the scSAT h
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Hunter, N. R., I. R. MacGregor, J. Dawes, and D. S. Pepper. "MICROCARRIER CULTURE OF HUMAN ENDOTHELIAL CELL TYPES - A SOURCE OF METABOLITES." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643348.

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The production of human endothelial cell secretory products in amounts sufficient for biochemical studies is largely restricted by the culture growth area. Conventional flat bed systems yield at best 20-30 x 106 cells per 180cm2 culture flask. To overcome this problem, cells may be grown on Cytodex 3 microcarriers allowing large numbers of cells to be grown and conditioned in small culture volumes. A typical microcarrier unit will contain 200-300 x 106 cells and may be expanded in excess of 1000 x 106 cells at confluence. High viability (95%) and recovery (70-80%) in sub-culturing of microcarr
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Mignone, Lindsay F., Shirley Masand, Jeffrey D. Zahn, and David I. Shreiber. "A Simple, Cost-Effective Method to Improve Cell Viability in Microniche Culture Systems." In ASME 2010 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2010. http://dx.doi.org/10.1115/sbc2010-19189.

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Microfluidic networks are increasingly used to generate custom microenvironmental niches for cell culture and assays of cellular behavior. Perfusion systems are typically required to overcome diffusive limitations associated with culturing cells longer than a few hours when nutrient delivery, oxygen delivery and metabolic waste removal are required to maintain cell viability. In addition to the added complexity of experimental methods, perfusion systems can result in nonuniform nutrient delivery and subject cells to shear stresses, which may alter cell behavior and possibly cause cell death. I
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Shimomura, Yuya, Shoichiro Kanno, Kenta Shimba, Yoshitaka Miyamoto, and Tohru Yagi. "Position-controllable cell culture substrate for adherent cells." In 2023 15th Biomedical Engineering International Conference (BMEiCON). IEEE, 2023. http://dx.doi.org/10.1109/bmeicon60347.2023.10321824.

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Reports on the topic "Cell culture"

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Oldberg, Nick. A Calculator for Cell Culture Plating. ResearchHub Technologies, Inc., 2024. http://dx.doi.org/10.55277/researchhub.mg3bsfrd.

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Leung, Diana. A Call for Context (in Cell Culture). New Science, 2022. http://dx.doi.org/10.56416/021uwn.

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Brudos, Emma, Miranda Nelson, and David Estrada. Optimizing Three-Dimensional Bioprinting for Cell Culture Scaffolds. Peeref, 2022. http://dx.doi.org/10.54985/peeref.2207p5266153.

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Techakumphu, Mongkol, Pranee Numchaisrika, Ratajuk Rungsiwiwat, Somchai Suwajanakorn, Kamtorn Pruksananonda, and Pramuan Virutamasen. Development of somatic cell nuclear transfer techique for biomedical and agricultural research : report. Chulalongkorn University, 2004. https://doi.org/10.58837/chula.res.2004.85.

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To develop somatic cell nuclear transfer by using the rabbit as a mode in biomedical and agricultural research. The experiments are described in nine chapters: Chapter 1: Introduction, Chapter 2: Superovulation and oocyte recipient production in rabbits, Chapter 3: A technique for adult and foetal fibroblast cell culture, for donor cell preparation in somatic nuclear transfer, Chapter 4: Preliminary studies on somatic cell nuclear transfer in rabbits, Chapter 5: In vitro development of rabbit cloned embryos using adult fibroblast cells, after post-activation treatment with cyelohexamide and 6-
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Malik, Abir, D. Lam, H. A. Enright, S. K. G. Peters, B. Petkus, and N. O. Fischer. Characterizing the Phenotypes of Brain Cells in a 3D Hydrogel Cell Culture Model. Office of Scientific and Technical Information (OSTI), 2018. http://dx.doi.org/10.2172/1466140.

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Scott, C. D., and D. K. Dougall. Plant cell tissue culture: A potential source of chemicals. Office of Scientific and Technical Information (OSTI), 1987. http://dx.doi.org/10.2172/5938126.

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Ostry, M. E., and K. T. Ward. Bibliography of Populus cell and tissue culture. U.S. Department of Agriculture, Forest Service, North Central Forest Experiment Station, 1991. http://dx.doi.org/10.2737/nc-gtr-146.

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Novaro, Virginia, and Mina BIssell. A Cell Culture Model for Understanding Estrogen Receptor Regulation in Normal and Malignant Cells. Defense Technical Information Center, 1998. http://dx.doi.org/10.21236/ada373393.

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Mort, A. (The structure of pectins from cotton suspension culture cell walls). Office of Scientific and Technical Information (OSTI), 1990. http://dx.doi.org/10.2172/7003410.

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Evangelatov, Alexander, Diana Naidenova, George Georgiev, Albena Momchilova, and Roumen Pankov. Effects of Hyperglycemia on Wound Healing in Threedimensional Cell Culture. "Prof. Marin Drinov" Publishing House of Bulgarian Academy of Sciences, 2021. http://dx.doi.org/10.7546/crabs.2021.06.08.

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