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Journal articles on the topic 'Cephalosporin C'

Author: Grafiati

Published: 4 June 2021

Last updated: 26 July 2025

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1

Hardianto, Dudi, Bima Wedana Isdiyono, and Fransiskus Xaverius Ivan. "BIOKONVERSI SEFALOSPORIN C MENJADI ASAM 7-AMINOSEFALOSPORANAT DENGAN SEFALOSPORIN ASILASE." Jurnal Bioteknologi & Biosains Indonesia (JBBI) 3, no. 2 (2016): 89. http://dx.doi.org/10.29122/jbbi.v3i2.139.

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Cephalosporins are the most widely used class of β-lactam antibiotic in the world and clinically active against gram positive and gram negative bacteria. Cephalosporin C (CPC) is naturally produced by fungus Cephalosporiun acremonium. CPC has moderate antibacterial activity with minimum inhibitory concentration values of 25-100 µg/mL and 12-25 µg/mL for gram-positive and for gram-negative bacteria, respectively. CPC can be converted into 7-aminocephalosporonic acid (7-ACA) as intermediate compound for cephalosporin derivatives by two-steps or one-step enzymatic method. Two-step enzymatic metho

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2

Xiao, X., S. Wolfe та A. L. Demain. "Purification and characterization of cephalosporin 7α-hydroxylase from Streptomyces clavuligerus". Biochemical Journal 280, № 2 (1991): 471–74. http://dx.doi.org/10.1042/bj2800471.

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Cephalosporin 7 alpha-hydroxylase, which catalyses the conversion of cephalosporins into their 7 alpha-hydroxy derivatives, was purified nearly 390-fold from Streptomyces clavuligerus through ion-exchange chromatography, (NH4)2SO4 fractionation, gel filtration and dye chromatography, with the use of h.p.l.c. to monitor enzyme activity. The nearly pure enzyme migrates as a single major band, with an Mr of 32,000 in SDS/PAGE. Its optimum pH is in the range 7.3-7.7. Under our conditions the reaction was fastest at temperatures in the range 20-30 degrees C. The Km for cephalosporin C is 0.72 mM, a

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3

Nour El-Din,, Mona, M. Saleh, M. Rasmy, and G. Ibrahim. "ANTIMICROBIAL EFFECT OF CEPHALOSPORIN-C PRODUCED BY Cephalosporium maydis." Journal of Plant Production 26, no. 11 (2001): 6813–23. http://dx.doi.org/10.21608/jpp.2001.257991.

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4

Balakrishnan, Nataraj, Sadhasivam Ganesan, Padma Rajasekaran, Lingeshwaran Rajendran, Sivaprasad Teddu, and Micheal Durairaaj. "Modified Deacetylcephalosporin C Synthase for the Biotransformation of Semisynthetic Cephalosporins." Applied and Environmental Microbiology 82, no. 13 (2016): 3711–20. http://dx.doi.org/10.1128/aem.00174-16.

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ABSTRACTDeacetylcephalosporin C synthase (DACS), a 2-oxoglutarate-dependent oxygenase synthesized byStreptomyces clavuligerus, transforms an inert methyl group of deacetoxycephalosporin C (DAOC) into an active hydroxyl group of deacetylcephalosporin C (DAC) during the biosynthesis of cephalosporin. It is a step which is chemically difficult to accomplish, but its development by use of an enzymatic method with DACS can facilitate a cost-effective technology for the manufacture of semisynthetic cephalosporin intermediates such as 7-amino-cephalosporanic acid (7ACA) and hydroxymethyl-7-amino-ceph

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5

Braña, Alfredo F., Saul Wolfe, and Arnold L. Demain. "Ammonium repression of cephalosporin production by Streptomyces clavuligerus." Canadian Journal of Microbiology 31, no. 8 (1985): 736–43. http://dx.doi.org/10.1139/m85-138.

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Production of β-lactam antibiotics took place during growth of Streptomyces clavuligerus in chemically defined medium. The specific activities of isopenicillin N synthetase ("cyclase"), isopenicillin N epimerase, and deacetoxycephalosporin C synthetase ("expandase") increased during the exponential phase of growth. Specific cephalosporin productivity during fermentation followed a similar pattern, reaching a maximum near the end of the growth phase and decaying rapidly in the stationary phase. Ammonium chloride depressed cephalosporin production, presumably as a result of repression of cyclase

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6

Botnarciuc, Mihaela, Irina Stan, and Sorina Ispas. "Cephalosporin resistant bacterial strains isolated from respiratory infections." ARS Medica Tomitana 21, no. 1 (2015): 7–11. http://dx.doi.org/10.1515/arsm-2015-0012.

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Abstract Objectives: The objective of the study is the evaluation of the actual resistance to second, third, and fourth generation cephalosporins over bacterial strains isolated from respiratory infections. The main causes for cephalosporin resistance of pathogenic and conditioned pathogen bacteria are: widespread usage, and impair immune response. Materials and methods: The analyzed specimens were throat swabs and sputum, from adult patients. The tests were performed using disk diffusion technique. We tested the following cephalosporin: From second generation: cefuroxime axetil; from third ge

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7

Mustika, Indria Puti, and Ahmad Wibisana. "PERAN MUTASI GEN ACY II TERHADAP PRODUKSI ANTIBIOTIK SEFALOSPORIN." Jurnal Bioteknologi & Biosains Indonesia (JBBI) 4, no. 2 (2017): 96. http://dx.doi.org/10.29122/jbbi.v4i2.2272.

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The Roles of AcyII Gene Mutations for Production of Antibiotics Derived From CephalosporinSemisynthetic antibiotics cephalosporins are widely used to treat infectious diseases, especially those caused by gram-negative bacteria. Various types of semisynthetic antibiotics could be synthesized using 7-aminocephalosporanic acid (7-ACA) as the main raw material. 7-ACA is obtained by conversion of cephalosporin C, either chemically or enzymatically. Converting cephalosporin C to 7-ACA enzymatically in one step involves the cephalosporin acylase enzyme. Currently, all of cefalosporin acylase enzymes

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8

Baldwin, Jack E., Robert M. Adlington, Nicholas P. Crouch, and Christopher J. Schofield. "The enzymatic conversion of exomethylene cephalosporin c into deacetyl cephalosporin c and the role of molecular oxygen in cephalosporin c biosynthesis." Tetrahedron 44, no. 2 (1988): 643–50. http://dx.doi.org/10.1016/s0040-4020(01)85852-x.

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9

Hano, Tadashi, Michiaki Matsumoto, Takaaki Ohtake, and Fumiaki Hori. "Reactive extraction of cephalosporin C." JOURNAL OF CHEMICAL ENGINEERING OF JAPAN 25, no. 3 (1992): 293–97. http://dx.doi.org/10.1252/jcej.25.293.

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10

Atroshenko, Denis L., Mikhail D. Shelomov, Sophia A. Zarubina, et al. "Multipoint TvDAAO Mutants for Cephalosporin C Bioconversion." International Journal of Molecular Sciences 20, no. 18 (2019): 4412. http://dx.doi.org/10.3390/ijms20184412.

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d-amino acid oxidase (DAAO, EC 1.4.3.3) is used in many biotechnological processes. The main industrial application of DAAO is biocatalytic production of 7-aminocephalosporanic acid from cephalosporin C with a two enzymes system. DAAO from the yeast Trigonopsis variabilis (TvDAAO) shows the best catalytic parameters with cephalosporin C among all known DAAOs. We prepared and characterized multipoint TvDAAO mutants to improve their activity towards cephalosporin C and increase stability. All TvDAAO mutants showed better properties in comparison with the wild-type enzyme. The best mutant was TvD

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11

Usher, John J., MaryAnn Lewis, Doris W. Hughes, and Bruce J. Compton. "Development of the cephalosporin C fermentation taking into account the instability of cephalosporin C." Biotechnology Letters 10, no. 8 (1988): 543–48. http://dx.doi.org/10.1007/bf01027126.

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12

Wren, Callyn Mariah, Jill Cowper, Nickie Greer, Laurel Goldin, and Alicia Perry. "Effect of Reduced Fluoroquinolone Use on Cephalosporin Use, Susceptibilities and Clostridioides difficile Infections." Antibiotics 11, no. 10 (2022): 1312. http://dx.doi.org/10.3390/antibiotics11101312.

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Background: Overuse of fluoroquinolones has led to concerning rates of resistance, particularly among Gram-negative organisms. They are also highly implicated as a risk factor for Clostridioides difficile infection, and reports of other serious adverse events led to recommendations to restrict their use. Our health system began targeting the reduction in unnecessary fluoroquinolone prescribing in 2018, aiming to promote their safe and effective use. Broad-spectrum cephalosporins are often used as an alternative to fluoroquinolones. We sought to evaluate whether decreased fluoroquinolone use wa

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13

Ullán, Ricardo V., Fernando Teijeira, Susana M. Guerra, Inmaculada Vaca, and Juan F. Martín. "Characterization of a novel peroxisome membrane protein essential for conversion of isopenicillin N into cephalosporin C." Biochemical Journal 432, no. 2 (2010): 227–36. http://dx.doi.org/10.1042/bj20100827.

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The mechanisms of compartmentalization of intermediates and secretion of penicillins and cephalosporins in β-lactam antibiotic-producing fungi are of great interest. In Acremonium chrysogenum, there is a compartmentalization of the central steps of the CPC (cephalosporin C) biosynthetic pathway. In the present study, we found in the ‘early’ CPC cluster a new gene named cefP encoding a putative transmembrane protein containing 11 transmembrane spanner. Targeted inactivation of cefP by gene replacement showed that it is essential for CPC biosynthesis. The disrupted mutant is unable to synthesize

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14

Tong, Shuangming, Linlin Zhu, Xiaona Wang, Xi LI, Yanhong Chang, and Hui Luo. "Optimization of Cephalosporin C Acylase Immobilization." E3S Web of Conferences 78 (2019): 02003. http://dx.doi.org/10.1051/e3sconf/20197802003.

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Cephalosporin C Acylase (CCA), the key biocatalyst in one-step enzymatic production of 7- amino cephalosporanic acid (7-ACA), was immobilized by amino-activated carrier (LX-1000HA) and epoxy-activated carriers (ES-103B, LX-1000EPC), and the activity was assayed. ES-103B carriers showed an advantage than the others. The amount of free enzyme and salt concentration were tested, and the optimum conditions were 1400U/g and 0.9M. In addition, two different methods (by shaker and packed column) were used in CCA immobilization, and the results demonstrated that the former obtained a higher immobilize

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15

Babu, Setty Mallikarjuna, and Subramania Ranganathan. "The total synthesis of Cephalosporin C." Resonance 19, no. 7 (2014): 649–53. http://dx.doi.org/10.1007/s12045-014-0067-1.

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16

Hicketier, M., and K. Buchholz. "Fluidized bed adsorption of Cephalosporin C." Journal of Biotechnology 93, no. 3 (2002): 253–68. http://dx.doi.org/10.1016/s0168-1656(01)00408-4.

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17

Despande, Bhagwant S., Sudha S. Ambedkar, and Jaiprakash G. Shewale. "Monitoring of cephalosporin C during bioconversion." Applied Biochemistry and Biotechnology 60, no. 3 (1996): 245–50. http://dx.doi.org/10.1007/bf02783587.

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18

Sohn, Young-Sun, Keun-Cheol Lee, Young-Hwan Koh, and Gwang-Hoon Gil. "Changes in Cellular Fatty Acid Composition of Cephalosporium acremonium during Cephalosporin C Production." Applied and Environmental Microbiology 60, no. 3 (1994): 947–52. http://dx.doi.org/10.1128/aem.60.3.947-952.1994.

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19

Skatrud, Paul L., Anthony J. Tietz, Thomas D. Ingolia, et al. "Use of Recombinant DNA to Improve Production of Cephalosporin C By Cephalosporium acremonium." Nature Biotechnology 7, no. 5 (1989): 477–85. http://dx.doi.org/10.1038/nbt0589-477.

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20

Park, Hong-Je, and Yong-Ho Khang. "Production of cephalosporin C by immobilized Cephalosporium acremonium in polyethyleneimine-modified barium alginate." Enzyme and Microbial Technology 17, no. 5 (1995): 408–12. http://dx.doi.org/10.1016/0141-0229(94)00076-4.

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21

Teijeira, Fernando, Ricardo V. Ullán, Susana M. Guerra, Carlos García-Estrada, Inmaculada Vaca, and Juan F. Martín. "The transporter CefM involved in translocation of biosynthetic intermediates is essential for cephalosporin production." Biochemical Journal 418, no. 1 (2009): 113–24. http://dx.doi.org/10.1042/bj20081180.

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The cluster of early cephalosporin biosynthesis genes (pcbAB, pcbC, cefD1, cefD2 and cefT of Acremonium chrysogenum) contains all of the genes required for the biosynthesis of the cephalosporin biosynthetic pathway intermediate penicillin N. Downstream of the cefD1 gene, there is an unassigned open reading frame named cefM encoding a protein of the MFS (major facilitator superfamily) with 12 transmembrane domains, different from the previously reported cefT. Targeted inactivation of cefM by gene replacement showed that it is essential for cephalosporin biosynthesis. The disrupted mutant accumu

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22

Preston, Sandra L., and Laurie L. Briceland. "Intrathecal Administration of Amikacin for Treatment of Meningitis Secondary to Cephalosporin-Resistant Escherichia Coli." Annals of Pharmacotherapy 27, no. 7-8 (1993): 870–73. http://dx.doi.org/10.1177/106002809302700709.

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OBJECTIVE: To report a case of gram-negative bacillary meningitis (GNBM) secondary to cephalosporin-resistant Escherichia coli that was treated with intrathecal and intravenous amikacin and intravenous imipenem/cilastatin (I/C). CASE SUMMARY: A patient who had undergone two recent neurosurgical procedures developed GNBM and bacteremia. He was treated empirically with ceftazidime. Both bloodstream and cerebrospinal fluid isolates were identified as E. coli, resistant to third-generation cephalosporins, penicillins, tobramycin, and gentamicin. The patient was subsequently treated with intravenou

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23

Cummings, Maxwell D., David P. Czajkowski, John Brunstein та ін. "2α-Alkoxymethyl Cephalosporins: Reactions of exo-2-Methylene Cephalosporin Sulfones with Alcohols". Collection of Czechoslovak Chemical Communications 59, № 10 (1994): 2282–92. http://dx.doi.org/10.1135/cccc19942282.

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A series of 2α-alkoxymethyl cephem sulfones were prepared by nucleophilic addition of a variety of alcohols to exo-2-methylene cephem sulfones. The 2α-alkoxymethyl group was introduced with the aim of improving the inhibitory activity against human leukocyte elastase (HLE) over the unsubstituted compounds. However, against HLE the in vitro activity was still inferior to that shown by the C-2 unsubstituted cephem sulfones.

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24

Zhang, Jinyou, Saul Wolfe та Arnold L. Demain. "Ammonium ions repress δ-(L-α-aminoadipyl)-L-cysteinyl-D-valine synthetase in Streptomyces clavuligerus NRRL 3585". Canadian Journal of Microbiology 35, № 3 (1989): 399–402. http://dx.doi.org/10.1139/m89-061.

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Production of cephems (predominantly cephamycin C) by Streptomyces clavuligerus grown in chemically defined medium supplemented with 120 mM NH4Cl was sharply reduced. This concentration of ammonium ions in the medium repressed δ-(L-α-aminoadipyl)-L-cysteinyl-D-valine (ACV) synthetase formation by about 75%. Of the other cephalosporin synthases, cyclase was repressed by 70%, expandase by 50%, and epimerase only to a very small extent. Inhibition of the action of ACV synthetase was only slight in the presence of 100 mM NH4Cl. Repression of ACV synthetase, cyclase, and expandase appears to be the

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25

Mazzella, L. J., та R. F. Pratt. "Effect of the 3′-leaving group on turnover of cephem antibiotics by a class C β-lactamase". Biochemical Journal 259, № 1 (1989): 255–60. http://dx.doi.org/10.1042/bj2590255.

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It has been previously demonstrated for class A beta-lactamases and the DD-peptidase of Streptomyces R61 that the presence of a leaving group at the 3′-position of a cephalosporin can lead to the generation of more-inert acyl-enzyme intermediates than from cephalosporins lacking such a leaving group, and thus to beta-lactamase inhibitors and potentially better antibiotics. In the present work we extend this result to a class C beta-lactamase, that of Enterobacter cloacae P99. The effect is not seen with first-generation cephalosporins, since here deacylation generally seems faster than elimina

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26

Hardianto, Dudi, Juwartina Royani, and Anna Safarrida. "Cephalosporin C Acylase from Microbes for One-step Enzymatic Transformation of Cephalosporin C to 7-Aminocephalosporanic Acid." Journal of Pure and Applied Microbiology 10, no. 4 (2016): 2495–99. http://dx.doi.org/10.22207/jpam.10.4.03.

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27

Ren, Yu, Yulin Lei, and Yushan Zhu. "Site-Directed Mutagenesis of Cephalosporin C Acylase and Enzymatic Conversion of Cephalosporin C to 7-Aminocephalosporanic Acid." Turkish Journal of Biochemistry 39, no. 1 (2014): 51–56. http://dx.doi.org/10.5505/tjb.2014.48569.

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28

Chin, J. F. Y., and J. Nandong. "Modelling, optimization and control of continuous two-stage Cephalosporin C production." IOP Conference Series: Materials Science and Engineering 1195, no. 1 (2021): 012037. http://dx.doi.org/10.1088/1757-899x/1195/1/012037.

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Abstract Cephalosporin is one of the most consumed antibiotics for its effectiveness against a wide variety of infections. Most cephalosporin products are the semi-derivatives of Cephalosporin C (CPC), a metabolite of the fungus Acremonium chrysogenum. Since naturally the desired metabolite is not produced in a large amount by the fungus, an innovative operational strategy is required to increase its yield for the production of the antibiotic to be economically feasible. One way to increase the cephalosporin productivity is by increasing the concentration of thin hyphae cell in the bioreactor,

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29

Felici, A., M. Perilli, N. Franceschini, et al. "Sensitivity of Aeromonas hydrophila carbapenemase to delta3-cephems: comparative study with other metallo-beta-lactamases." Antimicrobial Agents and Chemotherapy 41, no. 4 (1997): 866–68. http://dx.doi.org/10.1128/aac.41.4.866.

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Ceftriaxone and ceftriaxone S-oxide behaved as inactivators against the metallo-beta-lactamase of Aeromonas hydrophila AE036 and as substrates for the zinc beta-lactamase produced by Bacillus cereus (569/H/9) and Stenotrophomonas maltophilia ULA 511. Moreover, RO 09-1428, a catechol-cephalosporin, was not recognized by the A. hydrophila enzyme. Panipenem, cephalosporin C, cephalosporin C-gamma-lactone, and loracarbef were substrates for the three studied beta-lactamases.

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30

Prabandari, Erwahyuni, Dyah Noor Hidayati, Diana Dewi, Eni Dwi Islamiati, and Khaswar Syamsu. "PENINGKATAN PRODUKSI SEFALOSPORIN C DARI Acremonium chrysogenum CB2/11/1.10.6 DENGAN OPTIMASI MEDIA MENGGUNAKAN METODE RESPON PERMUKAAN." Jurnal Bioteknologi & Biosains Indonesia (JBBI) 4, no. 1 (2017): 10. http://dx.doi.org/10.29122/jbbi.v4i1.1808.

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Cephalosporin is a β-lactam antibiotic produced by Acremonium chrysogenum using submerged fermentation. Carbon and nitrogen are the most influential medium ingredients for cephalosporin formation. The purpose of this study was to obtain the best composition of media for cephalosporin C production. Response surface methodology was used for production optimization. The results showed that molasses of 70 g/Lwas the best carbon source, while the best nitrogen source was the combination of corn steep liquor, urea and ammonium sulphate. DL-methionine, carbon, and nitrogen source significantly affect

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31

Li, Ruichao, Dachuan Lin, Kaichao Chen, Marcus Ho Yin Wong та Sheng Chen. "First Detection of AmpC β-LactamaseblaCMY-2on a Conjugative IncA/C Plasmid in a Vibrio parahaemolyticus Isolate of Food Origin". Antimicrobial Agents and Chemotherapy 59, № 7 (2015): 4106–11. http://dx.doi.org/10.1128/aac.05008-14.

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ABSTRACTVibrio parahaemolyticusis an important causative agent of gastroenteritis, with the consumption of contaminated seafood being the major transmission route. Resistance to penicillin is common amongV. parahaemolyticusstrains, whereas cephalosporin resistance remains rare. In an attempt to assess the current prevalence and characteristics of antibiotic resistance of this pathogen in common food samples, a total of 54 (17% of the total samples)V. parahaemolyticusstrains were isolated from 318 meat and seafood samples purchased from supermarkets and wet markets in Shenzhen, China, in 2013.

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32

Lin, Xuemei, Jan Lambertz, Tim A. Dahlmann, Marc M. Nowaczyk, Burghard König, and Ulrich Kück. "A Straightforward Approach to Synthesize 7-Aminocephalosporanic Acid In Vivo in the Cephalosporin C Producer Acremonium chrysogenum." Journal of Fungi 8, no. 5 (2022): 450. http://dx.doi.org/10.3390/jof8050450.

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The pharmaceutical industry has developed various highly effective semi-synthetic cephalosporins, which are generated by modifying the side chains of the core molecule 7-aminocephalosporanic acid (7-ACA). In industrial productions, the 7-ACA nucleus is obtained in vitro from cephalosporin C (CPC) by chemical or enzymatic processes, which are waste intensive and associated with high production costs. Here, we used a transgenic in vivo approach to express bacterial genes for cephalosporin C acylase (CCA) in the CPC producer Acremonium chrysogenum. Western blot and mass spectrometry analyses veri

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33

Lin, Xuemei, Jan Lambertz, Tim A. Dahlmann, Marc M. Nowaczyk, Burghard König, and Ulrich Kück. "A Straightforward Approach to Synthesize 7-Aminocephalosporanic Acid In Vivo in the Cephalosporin C Producer Acremonium chrysogenum." Journal of Fungi 8, no. 5 (2022): 450. http://dx.doi.org/10.3390/jof8050450.

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The pharmaceutical industry has developed various highly effective semi-synthetic cephalosporins, which are generated by modifying the side chains of the core molecule 7-aminocephalosporanic acid (7-ACA). In industrial productions, the 7-ACA nucleus is obtained in vitro from cephalosporin C (CPC) by chemical or enzymatic processes, which are waste intensive and associated with high production costs. Here, we used a transgenic in vivo approach to express bacterial genes for cephalosporin C acylase (CCA) in the CPC producer Acremonium chrysogenum. Western blot and mass spectrometry analyses veri

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34

Zafira, Indira Zahra, and Jobrun Nandong. "Optimal feeding strategy of Cephalosporin C fermentation." IOP Conference Series: Materials Science and Engineering 495 (June 7, 2019): 012107. http://dx.doi.org/10.1088/1757-899x/495/1/012107.

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35

Pollegioni, Loredano, Elena Rosini, and Gianluca Molla. "Cephalosporin C acylase: dream and(/or) reality." Applied Microbiology and Biotechnology 97, no. 6 (2013): 2341–55. http://dx.doi.org/10.1007/s00253-013-4741-0.

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36

YAMADA, HISASHI, YOSHINORI ISHII, YUJI NOGUCHI, TOSHIKO MIURA, THORU MORI, and YOSHIMASA SAITO. "Protein Engineering of a Cephalosporin C Acylase." Annals of the New York Academy of Sciences 799, no. 1 Enzyme Engine (1996): 74–81. http://dx.doi.org/10.1111/j.1749-6632.1996.tb33181.x.

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37

Yang, Wu-Yung, Chun-Der Lin, I.-Ming Chu, and Chau-Jen Lee. "Extraction of cephalosporin C from whole broth and separation of desacetyl cephalosporin C by aqueous two-phase partition." Biotechnology and Bioengineering 43, no. 6 (1994): 439–45. http://dx.doi.org/10.1002/bit.260430602.

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38

Bent, Zachary W., та Glenn M. Young. "Contribution of BlaA and BlaB β-Lactamases to Antibiotic Susceptibility of Yersinia enterocolitica Biovar 1B". Antimicrobial Agents and Chemotherapy 54, № 9 (2010): 4000–4002. http://dx.doi.org/10.1128/aac.01754-09.

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ABSTRACT Highly pathogenic Yersinia enterocolitica biovar 1B produces two distinct β-lactamases, BlaA and BlaB. Mutants of a representative biovar 1B isolate were constructed and evaluated to determine the extent of limitation of susceptibility to broad-spectrum β-lactam antibiotics by BlaA and BlaB. The results demonstrated that BlaA, a class A enzyme, plays a significant role in limiting susceptibility to penicillins and cephalosporins. The contribution of BlaB, a class C enzyme, was less profound and was limited primarily to cephalosporin susceptibility.

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39

Tarek, A. El-bashiti. "Production of Cephalosporin C from Acremonium chrysogenum, and its Antimicrobial Activity against Some Pathogenic Bacteria." Pharmaceutical and Chemical Journal 4, no. 2 (2017): 19–23. https://doi.org/10.5281/zenodo.13762620.

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The Production of cephalosporin C by <em>Acremonium chrysogenum</em>in flask contain fermentation medium, after that its extraction, purification and estimation was performed by Spectrophotometric Method. The antimicrobial activities of both the crude and purified cephalosporin C were performed against the test organisms by Agar Well Diffusion Method. Agar well diffusion method and the inhibition zones were recorded and evaluated the effectiveness of the antibiotic against gram positive bacteria (<em>Staphylococcus aereus</em> and <em>Bacillus subtilis</em>) and no effectiveness against gram n

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40

Ma, Xiaoqiang, Senwen Deng, Erzheng Su, and Dongzhi Wei. "One-pot enzymatic production of deacetyl-7-aminocephalosporanic acid from cephalosporin C via immobilized cephalosporin C acylase and deacetylase." Biochemical Engineering Journal 95 (March 2015): 1–8. http://dx.doi.org/10.1016/j.bej.2014.11.015.

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41

Lin, Po-Chun, and I.-Ming Chu. "Separation of cephalosporin C and desacetyl cephalosporin C by high speed counter-current chromatography in aqueous two-phase systems." Biotechnology Techniques 9, no. 8 (1995): 549–52. http://dx.doi.org/10.1007/bf00152441.

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42

Dreyer, Jacqueline, Heiko Eichhorn, Ernst Friedlin, Hubert Kürnsteiner, and Ulrich Kück. "A Homologue of the Aspergillus velvet Gene Regulates both Cephalosporin C Biosynthesis and Hyphal Fragmentation in Acremonium chrysogenum." Applied and Environmental Microbiology 73, no. 10 (2007): 3412–22. http://dx.doi.org/10.1128/aem.00129-07.

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ABSTRACT The Aspergillus nidulans velvet (veA) gene encodes a global regulator of gene expression controlling sexual development as well as secondary metabolism. We have identified the veA homologue AcveA from Acremonium chrysogenum, the major producer of the β-lactam antibiotic cephalosporin C. Two different disruption strains as well as the corresponding complements were generated as a prelude to detailed functional analysis. Northern hybridization and quantitative real-time PCR clearly indicate that the nucleus-localized AcVEA polypeptide controls the transcriptional expression of six cepha

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Hermas, Abou-Elhagag A., Abobakr Mohamed Elnady, and Reham M. Ali. "Corrosion inhibition of stainless steel in sulfuric acid solution containing sulfide ions." Anti-Corrosion Methods and Materials 66, no. 3 (2019): 360–68. http://dx.doi.org/10.1108/acmm-10-2018-2016.

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Purpose Although stainless steel (SS) has good corrosion resistance in most aqueous solutions, it suffers corrosion in some solutions which contain aggressive ions such as sulfide ions. This study aims to use some cephalosporins (cefotaxime, cephapirin and cefazolin) as corrosion inhibitors of commercial SS in 0.5 M H2SO4 solution containing sulfide ions at 30°C. Design/methodology/approach The study was carried out using weight loss method, potential-time, linear polarization, potentiodynamic polarization, electrochemical impedance measurements, scanning electron microscopy, Fourier transform

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Navrátilova, Pavlina, Ivana Borkovcova, Zora Stastkova, Ivana Bednarova, and Lenka Vorlova. "Effect of Cephalosporin Antibiotics on the Activity of Yoghurt Cultures." Foods 11, no. 18 (2022): 2751. http://dx.doi.org/10.3390/foods11182751.

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The presence of antibiotics in milk is a significant problem affecting the technological safety of dairy products. The aim of the study was to determine the sensitivity of yoghurt cultures to residual levels of selected cephalosporin antibiotics (cephalexin, cefoperazone, cefquinome, cefazolin, and ceftiofur). Five yoghurt cultures were selected containing strains of Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus. Artificially fortified milk samples (whole pasteurized milk; 85 °C; 3–5 s) with cephalosporins at a concentration of the maximum residue limit were used t

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Pollegioni, Loredano, Simona Lorenzi, Elena Rosini, et al. "Evolution of an acylase active on cephalosporin C." Protein Science 14, no. 12 (2005): 3064–76. http://dx.doi.org/10.1110/ps.051671705.

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Demain, Arnold L., and Jinyou Zhang. "Cephalosporin C Production byCephalosporium acremonium: The Methionine Story." Critical Reviews in Biotechnology 18, no. 4 (1998): 283–94. http://dx.doi.org/10.1080/0738-859891224176.

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REYES, F., M. J. MARTINEZ, and J. SOLIVERI. "Determination of cephalosporin-C amidohydrolase activity with fluorescamine." Journal of Pharmacy and Pharmacology 41, no. 2 (1989): 136–37. http://dx.doi.org/10.1111/j.2042-7158.1989.tb06412.x.

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Herold, T., T. Bayer, and K. Sch�gerl. "Cephalosporin C production in a stirred tank reactor." Applied Microbiology and Biotechnology 29, no. 2-3 (1988): 168–73. http://dx.doi.org/10.1007/bf00251697.

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Ghosh, A. C., S. Borthakur, M. K. Roy, and N. N. Dutta. "Extraction of cephalosporin C using supported liquid membrane." Separations Technology 5, no. 2 (1995): 121–26. http://dx.doi.org/10.1016/0956-9618(95)00114-l.

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Han, Kai Hua, Hui Luo, Yao Zhen Xie, et al. "Immobilization and Thermostability Characterization of Cephalosporin C Acylase." Advanced Materials Research 634-638 (January 2013): 682–88. http://dx.doi.org/10.4028/www.scientific.net/amr.634-638.682.

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Some kinds of epoxy supports, LX1000-EP(C), LX1000-EP(D), LKZ-116, LKZ-118 and LKZ-126 were utilized to covalently immobilize cephalosporin C (CPC) acylase, the key enzyme in the one-step enzymatic process of 7-aminocephalosporanic acid (7-ACA) production. After preliminary carrier screening, the immobilized CPC acylase with LKZ-118 as the support shows the highest activity (115 U/g) suggesting its potential application in industrial 7-ACA production. The conditions of CPC acylase immobilized on LKZ-118 to achieve higher activity and thermostability of the immobilized enzyme were optimized by

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