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1
Gentry, Retha D., Lisa Ousley, and Candice N. Short. "Nickel Allergic Contact Dermatitis: A Case Report." Digital Commons @ East Tennessee State University, 2017. https://dc.etsu.edu/etsu-works/7159.
Abstract:
A 22-year-old male college student presented to primary care with a pruritic rash on his mid abdomen for the past 2 weeks. He reported mild to moderate pruritus and had been scratching the area above his belt buckle.
2
Spriggs, Sandrine. "The role of glutathione in Allergic Contact Dermatitis." Thesis, University of Liverpool, 2017. http://livrepository.liverpool.ac.uk/3012684/.
Abstract:
Allergic Contact Dermatitis is a skin condition that affects up to twenty percent of the North American and Western European population. The molecular initiating event (MIE) of this type IV delayed hypersensitivity is the formation of antigenic species by covalent modification of endogenous proteins. Cysteine residues, though not the most prevalent residues in skin proteins, are prone to react with small electrophilic molecules and thus play a crucial role in protein haptenation. The role of cysteine is also very prominent in removal of electrophiles. Glutathione (GSH) is the most prominent antioxidant in cells and contains a cysteine residue, providing a free reactive thiol for GSH conjugation of electrophiles and their subsequent removal. It is also the co-factor of an important set of enzymes involved in the metabolic clearance system, glutathione S-transferases (GST). However, it remains unclear whether the reactivity of small exogenous electrophiles with intracellular GSH could be a key factor in determining the epidermal bioavailability of sensitising chemicals. GST activity has been demonstrated both in skin and in most in vitro skin equivalents but so far studies have focussed on specific chemical clearance. The GSH cycle (synthesis, conjugation, recycling) has not been comprehensively studied in the HaCaT cell line, which is often used as a surrogate model for human skin. We showed that the model sensitisers 2,4-dinitrohalobenzenes, reacting with thiols via a SNAr mechanism, depleted intracellular GSH within the first hour of exposure in HaCaT cells, in a dose dependent manner. Synthesis of de novo GSH was investigated 24 hours after treatment with a single non-toxic dose of 10 μM of three dinitrohalobenzenes. GSH concentration was found similar or slightly higher than the level measured in control cells for 1-chloro-2,4-dinitrobenzene (DNCB) and 1-fluoro-2,4-dinitrobenzene (DNFB) but not for 1- bromo-2,4-dinitrobenzene (DNBB), which appeared to partially prevent GSH repletion. This data inferred that the repletion step was the differentiating factor between chemicals belonging to the same reactivity domain. The toxicity of the halogen released in the cells was most likely the reason for observing these differences and this might have been missed if only the clearance kinetics were investigated. Activation of the nuclear factor E2-related factor 2 (Nrf2) pathway was concomitantly observed for all compounds within two hours, and at concentrations less than 10 μM. Nrf2 activation is the precursor event for the overproduction of Phase II metabolic enzymes such as GSTs, enzymes involved in the synthesis of GSH such as Glutamyl cysteine ligase (GCL) and Glutathione synthetase (GS) as well as GSH related enzymes acting against oxidative stress such as Glutathione Reductase (GR). HaCaT cells treated with the model aldehydes benzaldehyde and phenylacetaldehyde, which oxidised cysteine residues in chemico, did not suffer a significant depletion of GSH levels at sub-toxic concentrations. In addition, these aldehydes have been shown to react with amines via a Schiff base mechanism to form stable protein adducts. Our set of data indicated that the Schiff base mechanism was most probably favoured and that an oxidation of GSH (a reduction of the GSH:GSSG ratio), was effectively and rapidly controlled by GR activity. The volatility of aldehydes was likely to limit the bioavailability of these compounds intracellularly, possibly increasing the probability of haptenation with cell membrane proteins. Skin sensitisers are often defined by their potency (ranging from extreme to non-sensitising) rather than their chemical reactivity domain. We compared the clearance of two extreme sensitisers by the GSH pathway: DNCB and diphenylcyclopropenone (DPCP). We demonstrated that two chemicals able to react with thiols in chemico via different mechanisms (SNAr for DNCB and Michael addition for DPCP) were affecting GSH levels differently in HaCaT cells. DNCB induced GSH depletion at non-toxic concentrations, while exposure to DPCP decreased cell viability at concentrations for which GSH stock was not significantly reduced. DPCP reactivity with thiols has been suggested in cell membranes. Contrary to the results obtained for DPCP, diethyl maleate (DEM), a moderate sensitiser also reacting via Michael addition, was cleared by GSH at a rate similar to the rate of de novo GSH synthesis, masking the overall depletion of GSH. Exposure to DEM induced an overproduction of GSH after 24 hours that was more pronounced than the one observed for the more potent sensitiser DNCB, showing that the sensitising potency of chemicals could not be correlated solely to the ability to be conjugated by GSH (i.e. weak sensitisers would be cleared rapidly while potent sensitisers would not). These experiments demonstrated that the defence mechanisms in the HaCaT cell line were providing a rapid response to chemical stress. To investigate the effects of chemical exposure on GSH lifecycle in reconstructed human epidermis (RHE), we attempted repeated cycles of 2 hour exposure to DNCB over a week long period. For three consecutive treatments, each exposure to DNCB led to GSH depletion. Replenishment to basal level was observed after a 22 hour recovery period. Accumulation of Nrf2 in the cytosol also occurred within the two hours of exposure to DNCB but returned to baseline during each recovery period. The amount of GSH conjugate formed (dinitrophenyl glutathione) increased after each exposure, suggesting that the metabolic capacity of skin may be enhanced in response to exposure to exogenous compounds. In conclusion, the GSH cycle is fully active in skin and participates in the clearance of some exogenous compounds with electrophilic properties. GSH skin metabolism can also potentially be enhanced after repeated exposures to small quantities of electrophiles. Future risk assessments for skin sensitisation potential of topically applied chemicals could integrate these findings to correlate more realistically to in vivo scenarios.
3
Bensefa-Colas, Lynda. "Analyse des dermatoses professionnelles au sein du réseau national de vigilance et de prévention des pathologies professionnelles (RNV3P) : distribution, tendances et situations professionnelles à risque." Thesis, Sorbonne Paris Cité, 2015. http://www.theses.fr/2015USPCB155.
Abstract:
Contexte : Les dermatoses professionnelles sont l’une des principales causes de pathologies professionnelles en Europe, et dans près de 80% des cas, elles concernent des dermatites de contact professionnelles (DCP). Les DCP comprennent les dermatites allergiques de contact (DAC), les dermatites irritatives de contact (DIC) et les urticaires de contact (UC). En France, les secteurs et nuisances à risque de DCP et les tendances chronologiques de DCP sont très peu documentés. Objectifs : 1) étudier les tendances observées au fil du temps vis-à-vis du nombre de cas de dermatoses professionnelles, des secteurs d’activité concernés et des nuisances mises en cause, 2) mettre en évidence des secteurs ou des postes à risque de dermatoses professionnelles. Méthode : Ce travail de thèse s’appuie sur le réseau français RNV3P (Réseau national de vigilance et de prévention des pathologies professionnelles) qui assure le suivi des évènements de santé liés au travail sur l’ensemble du territoire, en centralisant les informations recueillies dans ses 32 centres de consultations de pathologies professionnelles (CCPP). Les analyses ont porté sur les DAC, les DIC et les UC en lien probable ou certain avec le travail et recensées dans le RNV3P entre 2001 et 2010. L’analyse des évolutions temporelles a été réalisée à partir des rapports de cote de signalement (Reporting Odds-Ratio, ROR). Deux méthodes statistiques ont été utilisées : le coefficient de corrélation de rang de Kendall sur les ROR estimés chaque année et le modèle logistique calculant la variation annuelle des cas. Résultats : Les 5990 cas de DCP notifiés touchent plus fréquemment les femmes, dans des tranches d'âges jeunes. Les principaux secteurs mis en cause pour les trois DCP se distribuent différemment en fonction du sexe et sont, par ordre décroissant de fréquence : santé et action sociale, services personnels, construction, métallurgie et travail des métaux, administrations publiques, services fournis principalement aux entreprises, commerce et réparation automobile, hôtellerie et restauration. Les DCP associées aux cosmétiques (savons ou substances parfumantes) et les biocides augmentent significativement dans le secteur de la santé et de l’action sociale ; les produits de coiffure et les cosmétiques (savons essentiellement) augmentent dans celui des services personnels, essentiellement parmi les coiffeuses. On observe une montée des cas de DAC aux résines époxy dans la construction sur la période et les DIC liées au ciment dans ce secteur ne baissent pas. Les cas de DAC aux métaux augmentent dans le secteur des services fournis aux entreprises (personnels de nettoyage). Nos résultats ont aussi montré une hausse des DAC liées aux isothiazolinones, quel que soit le secteur (+38%) et une baisse des DIC liées à l’action de lavage des mains (utilisation itérative des savons). Par ailleurs, nos données ont objectivé l’impact de mesures réglementaires ou de politiques visant à prévenir les DCP : la mise en place de la directive européenne « Ciment » (2003/53/CE) en 2005 s’est accompagnée d’une réduction de moitié des cas de DAC et une baisse similaire a été observée à partir des données du réseau britannique EPIDERM, confortant nos conclusions ; la campagne de substitution des gants en latex dans le secteur de soins au milieu des années 2000 est suivie d’une réduction significative de la notification des cas d’UC. Conclusion : Ces résultats démontrent, avec l’exemple des dermatoses professionnelles, la capacité du RNV3P à orienter les politiques nationales en matière de prévention des risques professionnels et à en évaluer l’efficacitéBackground: In Europe, occupational skin disease is one of the major causes of work-related diseases, among them occupational contact dermatitis (OCD) accounts for 80% of all cases of OSD reported, but data about sectors and agents at risk remains sparse. Objectives: 1) to study OCD trends in terms of industrial activities and main causal agents, 2) to describe industrial sectors or occupations at risk of OCD. Method: Data were collected from the French National Network of Occupational Disease Surveillance and Prevention (RNV3P) during 2001-2010 period, based on the 32 French Occupational and Environmental Disease Consultation Centres. All allergic contact dermatitis (ACD), irritative contact dermatitis (ICD) and contact urticaria (CU) probably or certainly work-related were included in the study. Trends were examined (i) on annual crude numbers of OCD and (ii) on reported odds-ratios of OCD calculated using logistic regression models. Results: 5990 OCD cases were included and concerned more frequently young age classes among women and older age classes among men. Industrial sectors more commonly involved in OCD concerned in decreasing order: health and social work activities, personal service activities, construction, metal industry, public administrations, other business activities, retail trade and repair of motor vehicles, and hotels and restaurants. According to trend analysis of OCD cases, cosmetics (including soaps and fragrances) and biocides increased significantly in health and social work activities; hairdressing products and cosmetics (mainly soaps) in personal service activities (including primarily hairdressers). In construction sector, ACD due to epoxy resins increased during the study period and concomitantly, ICD attributed to cement did not decrease underlying the lack of prevention in this sector at risk. ACD due to metals increased in other business activities mainly occupied by cleaning staff. We showed a rise of ACD due to isothiazolinone compounds whatever the sector. Besides, we have shown the impact of regulatory measures or prevention campaign on OCD: (i) a significant reduction by almost half in the occurrence of ACD attributed to chromate in cement which coincides with the implementation of the European Cement directive and we obtained similar results to a study based on the UK EPIDERM network; (ii) the effectiveness of latex exposure prevention measures for health care workers since half of 2000s and (iii) the wide use of disinfection with an alcohol-based solution in health and social work activity followed by a significant reduction of ICD due to iterative handwashing with soap. Conclusion: RNV3P data allowed to highlight causal agents involved in sectors most at risk of OCD. These results might help intervention policy in health and safety at work and allergy prevention. They also showed the effectiveness of preventative intervention on OCD, suggesting that RNV3P reporting scheme is useful in France to evaluate intervention policy in health and safety at work
4
Reis, Felipe Rovere Diniz 1979. "Dermatite alergica de contato ocupacional = perfil clinico-epidemiologico dos pacientes atendidos no ambulatorio de medicina do trabalho da Unicamp." [s.n.], 2010. http://repositorio.unicamp.br/jspui/handle/REPOSIP/310058.
Abstract:
Orientador: Jose Inacio de OliveiraDissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Ciencias Medicas
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Resumo: Diversos estudos descrevem o perfil clínico-epidemiológico e os resultados dos testes de contato das populações atendidas com suspeitas de dermatite alérgica de contato. Alguns evidenciam a provável relação causal com o trabalho; outros alertam para a necessidade de repetição do teste quando ocorrem múltiplas reações, principalmente nas demandas trabalhistas. O objetivo foi analisar o perfil clínico-epidemiológico e os resultados dos testes de contato dos pacientes com suspeita de dermatite alérgica de contato ocupacional atendidos no Ambulatório de Medicina do Trabalho da Universidade Estadual de Campinas. Foi realizado um estudo descritivo transversal retrospectivo com pacientes atendidos entre 1 de janeiro de 1999 e 31 de dezembro de 2008, envolvendo as seguintes variáveis: idade, cor, sexo, ocupação, tempo na ocupação, agente referido como sensibilizante, local inicial do eczema, origem do encaminhamento, resultados dos testes de contato e nexo causal. Foram analisados 180 prontuários e os resultados mostraram que o perfil clínicoepidemiológico foi semelhante àqueles referidos na literatura, mas os índices desensibilização foram proporcionalmente maiores: 85% dos pacientes tiveram pelo menos uma reação positiva no primeiro teste de contato, a média de reações positivas por teste foi 3,38 e 68% dos testes positivos apresentaram mais de duas reações positivas. Os elevados índices de sensibilização e a existência simultânea de múltiplas reações na maioria dos pacientes podem ser explicados por condições predisponentes para polissensibilização - dermatite nas mãos cronificadas por repetidas exposições ocupacionais - e/ou pela interpretação de reações irritativas como reações positivas. Foi sugerida, então, uma Ficha de Coleta de Dados, a ser preenchida durante as avaliações clínicas e a repetição dos testes de contato quando ocorrerem mais de 2 reações positivas relevantes a substâncias não correlacionada
Abstract: Several studies describe the clinical and epidemiological profile and the results of patch tests of the targeted population with suspected allergic contact dermatitis. Some evidence the probable causal relationship to work; others warn of the need to repeat tests when multiple reactions occur, especially in the lawsuits. The objetive was to analyze the clinical and epidemiological profile and the results of patch tests of patients with suspected allergic contact dermatitis treated at the Campinas State University's Occupational Medicine Clinic. We conducted a retrospective cross-sectional study with patients treated between January 1, 1999 and December 31, 2008 involving the following variables: age, race, sex, occupation, time in occupation, sensitizing agent, eczema locus, referral source, results of patch tests and causal relationship. We analyzed 180 medical records and the results show that clinical and epidemiological profile was similar to those reported in the literature, but the rates of awareness were proportionally larger: 85% of patients had at least one positive reaction in the first patch test, the average of positive reactions per test was 3.38 and 68% dos positive tests had more than two positive reactions. High levels of sensitization and the simultaneous existence of multiple reactions in most patients can be explained by predisposing factors to polysensization - cronic dermatitis on the hands caused by repeated occupational exposure - and/or by the interpretation of irritant reactions as positive reactions. It has been suggested then a sheet data collection to use in the clinic evaluation that includes the repetition of tests when more than 2 relevant positive reactions occurred to substances not correlated
Mestrado
Epidemiologia
Mestre em Saude Coletiva
5
Färm, Gunilla. "Contact allergy to colophony : clinical and experimental studies with emphasis on clinical relevance /." Stockholm, 1997. http://www.kibic.ki.se/ki/diss/971107farm.html.
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Memon, Aamir Aziz. "Investigation of cellular and clinical aspects of allergic contact dermatitis." Thesis, University of Liverpool, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.309918.
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Beliauskienė, Aistė. "Dermatitu sergančių pacientų įsijautrinimo kontaktiniams alergenams ir su juo susijusių veiksnių tyrimas." Doctoral thesis, Lithuanian Academic Libraries Network (LABT), 2012. http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2012~D_20120214_092202-76017.
Abstract:
Darbo tikslas – įvertinti dermatitu sergančių pacientų įsijautrinimą kontaktiniams alergenams ir su juo susijusius veiksnius.
Darbo uždaviniai:
1. Nustatyti įsijautrinimo kontaktiniams alergenams dažnumą tarp dermatitu sergančių pacientų.
2. Įvertinti įsijautrinimo kontaktiniams alergenams dažnumo sąsajas su tiriamųjų amžiumi, lytimi ir profesija.
3. Nustatyti įsijautrinimo kontaktiniams alergenams klinikos ypatumus.
4. Įvertinti įsijautrinimo kontaktiniams alergenams sąsajas su atopiniu dermatitu, lėtine (-ėmis) kojos (-ų) opa (-omis) ir kitais veiksniais.
Išvados:
1. Kontaktiniams alergenams įsijautrinę pusė dermatitu sergančių pacientų, dažniausiai – metalui nikeliui (17,1 proc.). Iš aromatinių medžiagų dažniausias yra įsijautrinimas Peru balzamui (8 proc.). Į pagrindinį alergenų rinkinį neįtrauktini retai (0,1 proc.) nustatyti alergenai: gumų medžiagos merkaptobenzotiazolas ir N-zopropil-N’-fenil-parafenilendiaminas.
2. Įsijautrinimas kontaktiniams alergenams jaunesniems nei 40 metų dermatitu sergantiems pacientams pasireiškia du kartus dažniau nei vyresniems, o moterims – du kartus dažniau nei vyrams. Žemės ūkio ir miškininkystės darbuotojai kontaktiniams alergenams įsijautrinę tris kartus dažniau nei kiti dirbantieji. Vertinant atskirus alergenus, jaunesni nei 40 metų pacientai dažniau nei vyresni yra įsijautrinę nikeliui, kobaltui ir kvaterniumui-15, o 40 metų ir vyresni – Peru balzamui, kanifolijai, aromatų mišiniui I, lanolinui, seskviterpenlaktonams ir... [toliau žr. visą tekstą]The aim of the study: To evaluate contact sensitization and its associated factors in patients with dermatitis. The objectives of the study: 1. To determine the frequency of sensitization to contact allergens in patients with dermatitis. 2. To evaluate associations between the frequency of sensitization to contact allergens and age, sex and occupation in patients with dermatitis. 3. To determine clinical features of contact sensitization. 4. To evaluate associations between sensitization to contact allergens and atopic dermatitis, chronic leg ulcers and other factors. CONCLUSIONS 1.Half of the patients with dermatitis were sensitized to contact allergens, most frequently to metal nickel (17.1%). From the fragrances, bal¬sam of Peru was the most frequent contact allergen (8%). Rare allergens that should not be included in the baseline series are the substances present in the composition of rubber – mercaptobenzothiazole and N-isopropyl-N'-phenyl-p-phenylene diamine (IPPD). 2.Patients under 40 years of age are two times more frequently sensitized than the older ones. In women contact allergy is twice as more frequent as in men. Agricultural and forestry workers are sensitized to contact allergens three times as more often as other employees. What regards individual allergens, among patients under 40 years of age sensitization to nickel, cobalt and quaternium-15 is more prevalent than in the older ones. Among patients who are 40 years of age and over contact sensitization to... [to full text]
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Bondesson, Lundeberg Lena. "Interaction between the nervous and immune systems in allergic contact dermatitis : a clinical and experimental study with emphasis on the role of VIP and serotonin /." Stockholm, 1998. http://diss.kib.ki.se/search/diss.se.cfm?19980611bond.
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Kuzmina, Natalia. "Biophysical aspects of contact dermatitis and its prevention /." Stockholm, 2004. http://diss.kib.ki.se/2004/91-7349-767-3/.
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El-Nour, Husameldin. "Study of serotonin, innervation and sensory neuropeptides in allergic contact dermatitis /." Stockholm, 2005. http://diss.kib.ki.se/2005/91-7140-464-3/.
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McFadden, John. "Clinical and experimental aspects of allergic contact dermatitis to para-phenylenediamine." Thesis, University of Southampton, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.536290.
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Everness, Katharine Mary. "Allergic contact dermatitis to nickel : a study of antigen presentation in vitro." Thesis, University of Edinburgh, 1988. http://hdl.handle.net/1842/18869.
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Nyrén, Miruna. "Skin sensitivity testing : a biophysical approach /." Stockholm, 2002. http://diss.kib.ki.se/2002/91-7349-173-X.
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Silvestre, Marilene Chaves. "Avaliação da dermatite de contato alérgica ao níquel através da técnica de imuno-histoquímica." Universidade de São Paulo, 2017. http://www.teses.usp.br/teses/disponiveis/5/5133/tde-09082017-103915/.
Abstract:
A dermatite de contato alérgica (DCA) ao íon níquel (Ni2+) é uma dermatose inflamatória frequente nos países industrializados. Envolve a ativação de células T específicas ao Ni2+, seguida da proliferação e indução de um perfil misto de citocinas, tanto pró-inflamatórias quanto reguladoras, sugerindo que vários subtipos de células T (helper - Th e citotóxica - Tc) estão envolvidos na resposta imune. Este estudo teve como objetivo a análise das citocinas TNF-alfa, INF-y, IL-2, IL-4, IL-10, IL-13, IL-17 e IL-23 pela técnica de imuno-histoquímica, para tentar identificar a prevalência de um ou mais subtipos de células T (Th/Tc), nos eczemas crônico e agudo de pacientes com DCA ao Ni2+. Avaliamos 20 pacientes (17 mulheres e 3 homens, com idade mediana de 46 anos) apresentando eczema crônico, pelo contato cotidiano do paciente com o Ni2+. Foram coletadas duas biópsias de pele em cada um dos 20 pacientes, a primeira no local do eczema crônico ao Ni2+, antes da aplicação do teste de contato (TC); e a segunda no local do eczema agudo, provocado pelo TC com o sulfato de níquel, 48 horas após sua fixação, nas leituras positivo forte (++) ou positivo muito forte (+++). Foram 160 amostras de eczema agudo e 160 de eczema crônico, perfazendo um total de 320 amostras. Apenas três amostras foram excluídas devido a algum tipo de falha técnica, como, por exemplo, o descolamento dos cortes de pele da lâmina. Para a análise dos dados utilizou-se o software estatístico STATA versão 13. As amostras coradas revelaram resultados positivos para as oito citocinas estudadas, e estas apresentaram valores heterogêneos. Esta heterogeneidade foi medida pelo coeficiente de variação, indicando a variabilidade do conjunto dos dados obtidos. O TNF-alfa, IFN-y, IL-4, IL-13 e IL-17 tiveram prevalência maior no eczema crônico do que no eczema agudo, a IL-2 e IL-23 apresentaram maior prevalência no eczema agudo, em comparação com o eczema crônico e a IL-10 apresentou prevalência similar tanto no eczema agudo quanto no crônico, porém, estas prevalências foram muito baixas, em ambos os eczemas. O TNF-alfa foi a citocina que mais prevaleceu no eczema crônico e a IL-2 foi a mais prevalente no eczema agudo. Porém, estas prevalências foram estatisticamente significantes apenas para a IL-4 e IL-13. Verificamos, nos eczemas crônico e agudo, a presença de um perfil misto de citocinas dos subtipos de células T (Th/Tc), sugerindo que as respostas imunes são expressas ao mesmo tempo. Entretanto, são necessários mais estudos para uma compreensão mais ampla sobre o perfil das citocinas na DCA ao Ni2+, o que poderia levar a novas abordagens terapêuticasAllergic contact dermatitis (ACD) to nickel (Ni+2) is a inflammatory dermatosis, common in industrialized countries. It involves the activation of nickel-specific T cells, followed by the proliferation and induction of a mixed profile of both proinflammatory and regulatory cytokines, suggesting that several T cell subtypes (helper - Th and cytotoxic - Tc) are involved in the immune response. This study aimed to analyze the cytokines TNF-alfa, INF-y, IL-2, IL-4, IL-10, IL-13, IL-17 and IL-23 using the immunohistochemistry technique in order to try to identify the prevalence of one or more T cell subtypes (Th/Tc) in the chronic and acute eczema of patients with ACD to Ni+2. We evaluated 20 patients (17 women and 3 men, median age of 46 years) with chronic eczema, by the patient\'s daily contact with Ni+2. Two skin biopsies were collected in each of the 20 patients, the first at the site of the chronic eczema to Ni+2, prior to the application of the contact test (CT); and the second at the site of acute eczema caused by CT with nickel sulphate, 48 hours after its fixation in the strong positive (++) or very strong positive (+++) readings. There were 160 samples of acute eczema and 160 of chronic eczema, a total of 320 samples. Only three samples were excluded due to some kind of technical failure, such as detachment of the skin cuts from the microscope slide. Statistical software STATA version 13 was used to analyze the data. The stained samples showed positive results for the eight cytokines studied, and these presented heterogeneous values. This heterogeneity was measured by the coefficient of variation, indicating the variability of the data set obtained. TNF-alfa, IFN-y, IL-4, IL-13 and IL-17 had a higher prevalence in chronic eczema than in acute eczema, IL-2 and IL-23 were more prevalent in acute eczema compared to chronic eczema and IL-10 presented similar prevalence in both acute and chronic eczema, however, a very low prevalence in both eczema. TNF-alfa was the most prevalent cytokine in chronic eczema and IL-2 was the most prevalent in acute eczema. However, these prevalences were statistically significant only for IL-4 and IL-13. In chronic and acute eczema, we observed the presence of a mixed cytokine profile of the T cell subtypes (Th/Tc), suggesting that immune responses are expressed at the same time. However, further studies are needed for a broader understanding of the cytokine profile in ACD to Ni+2, which could lead to new therapeutic approaches
15
Little, Matthew Clifford. "The role of the keratinocyte in nickel- and chromium- induced allergic contact dermatitis." Thesis, University of Sheffield, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.267124.
16
Cheung, Connie Tsui-Ping. "Expression and localisation of cutaneous alcohol and aldehyde metabolising enzymes." Thesis, Imperial College London, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.342325.
17
Sjögren, Florence. "Dermal cell trafficking : from microscopy to microdialysis /." Linköping : Univ, 2005. http://www.bibl.liu.se/liupubl/disp/disp2005/med883s.pdf.
18
Bråred, Christensson Johanna. "Clinical and experimental studies on oxidized fragrance terpenes as contact allergens /." Göteborg : Department of Dermatology and Venereology, Sahlgrenska University Hospital, Institute of Clinical Sciences at Sahlgrenska Academy, University of Gothenburg, 2009. http://hdl.handle.net/2077/19052.
19
Mesquita, Ísis Fiorello de Oliveira. "Teste alérgico de contato nos portadores de úlceras crônicas de membros inferiores." Botucatu, 2019. http://hdl.handle.net/11449/191155.
Abstract:
Orientador: Luciana Patrícia Fernandes AbbadeResumo: Introdução: Úlceras crônicas de membros inferiores são muito prevalentes. O tratamento local é feito através do uso de curativos específicos e outros produtos tópicos. A utilização destes podem causar dermatite de contato alérgica, aumentando a morbidade relacionada à doença e retardo da cicatrização. Objetivos: Identificar a frequência e principais substâncias alergênicas relacionadas à dermatite de contato nos pacientes portadores de úlceras crônicas nos membros inferiores. Métodos: Estudo observacional, com desenho transversal, realizado em 64 pacientes com diagnóstico de úlcera crônica de membros inferiores ativa ou cicatrizada independente da etiologia. Em todos os participantes foi realizado o teste de contato com a bateria padrão e com os curativos e produtos frequentemente utilizados para tratamento destas úlceras. Foram avaliadas variáveis clínicas e as relacionadas ao teste de contato. Resultados: Eczema peri-úlcera estava presente em 50 pacientes (78,1%). O teste de contato padrão foi positivo em 28 participantes (43,8%), sendo o paraben mix, sulfato de níquel e lanolina as substâncias do teste padrão que mais positivaram. O teste de contato com os curativos e produtos foi positivo em 17 participantes (25,6%) e os principais alérgenos foram, em ordem decrescente: colagenase com cloranfenicol (16%), sulfadiazina de prata (13%) e bota de Unna (6%). Eczema peri-úlcera relacionou-se positivamente com o uso de colagenase e hidratantes. Conclusão: A positividade ao teste... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: Introduction: Chronic leg ulcers are very prevalent and the local treatment is done through the use of specific dressings and other topical products. The use of these can cause allergic contact dermatitis, increasing disease-related morbidity and delayed healing. Objective: To identify the frequency and the main allergenic substances related to contact dermatitis in patients with chronic leg ulcers. Methodology: Observational study, with transversal design, performed with 64 patients diagnosed with active or healed chronic leg ulcers regardless of etiology were included. Patch tests were performed with all the patients, including the standard battery and the dressings and products frequently used on the treatment. Clinical variables and those related to the patch test were evaluated. Results: Peri-wound eczema was present in 50 patients (78.1%). The standard patch test was positive in 28 (43.8%) participants, with paraben mix, nickel sulfate and lanolin being the most positive substances. The patch test with dressings and products was positive in 17 (25.6%) participants and the main allergens were, in decreasing order: collagenase with chloramphenicol (16%), silver sulfadiazine (13%) and Unna's boot (6%). Peri-wound eczema was positively related to the use of collagenase and moisturizers. Conclusion: Positive patch test in patients with chronic leg ulcers is still high and peri-wound eczema, an important comorbidity that delays healing, is at increased risk in patients using ... (Complete abstract click electronic access below)
Mestre
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Minang, Jacob. "Cytokine responses in metal-induced allergic contact dermatitis : Relationship to in vivo responses and implication for in vitro diagnosis." Doctoral thesis, Stockholm : Dept. of Immunology, Wenner-Gren Institute, Stockholm University, 2005. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-717.
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Hardenberg, Jan-Hendrik Bernhard [Verfasser]. "The αE(CD103)β7 integrin and its role on regulatory T-cells in allergic contact dermatitis / Jan-Hendrik Bernhard Hardenberg." Göttingen : Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2020. http://d-nb.info/1221367781/34.
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Ostrowski, Anja [Verfasser]. "Silica Nanoparticles in Mouse Models of Skin Diseases : Local Penetration, Systemic Distribution and Effect on Allergic Contact Dermatitis / Anja Ostrowski." Berlin : Freie Universität Berlin, 2015. http://d-nb.info/1064869769/34.
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Ljuba, Vujanović. "Kontaktna senzibilizacija kod obolelih od hronične venske insuficijencije." Phd thesis, Univerzitet u Novom Sadu, Medicinski fakultet u Novom Sadu, 2014. https://www.cris.uns.ac.rs/record.jsf?recordId=87660&source=NDLTD&language=en.
Abstract:
Pojava alergijskog kontatnog dermatitisa koji nastaje kao komplikacija tokom lečenja hronične venske insuficijencije je dobro poznata. Cilj istraživanja je bio da se kod obolelih od hronične venske insuficijencije utvrdi učestalost kontaktne senzibilizacije ekcemskog tipa, potom da li postoji rizik za nastanak kontaktne senzibilizacije kao i postojanje korelacije između dužine trajanja bolesti i kontaktne senzibilizacije. Hipoteza rada je da osobe sa hroničnom venskom insuficijencijom značajno češće razvijaju kontaktnu senzibilizaciju nego osobe bez hronične venske insuficijencije, da imaju veći rizik za nastanak kontaktne senzibilizacije kao i da je učestalost kontaktne senzibilizacije u pozitivnoj korelaciji sa dužinom trajanja bolesti. Istraživanje je sprovedeno nad 266 ispitanika. Formirane su dve grupe. Eksperimentalnu grupu su činili oboleli od hronične venske insuficijencije upućeni na alergološko testiranje pod sumnjom na postojanje kontaktnog dermatitisa, a kontrolnu grupu su činili bolesnici bez hronične venske insuficijencije epikutano testirani pod sumnjom na postojanje kontaktnog dermatitisa. Obolelima od hronične venske insuficijencije je procenjena težina bolesti na osnovu CEAP klasifikacije. Potom je svaki ispitanik podvrgnut alergološkom epikutanom testiranju pač testom. Istraživanje je pokazalo da je učestalost kontaktne senzibilizacije među obolelima od hronične venske insuficijencije iznosila 49,3%. Učestalost kontaktne senzibilizacije kod osoba sa hroničnom venskom insuficijencijom na alergene: iz sastava Evropske standardne baterije je iznosila 31,55%; baterije specifične za hroničnu vensku insuficijenciju je iznosila 28,45%; komercijalno dostupne baterije alergena ubikvitarnih korovskih biljaka iz porodice Compositae je iznosila 6,69%; originalno spravljene ekstrakte ubikvitarnih korovskih biljaka Vojvodine je iznosila 6,11%. Ne postoji statistički značajna razlika u odnosu na pol. Prevalencija kontaktne senzibilizacije kod osoba sa hroničnom venskom insuficijencijom se nije statistički značajno razlikovala od osoba koje nisu imale hroničnu vensku insuficijenciju. Pozitivnost epikutanog alergološkog testa kojim se potvrđuje postojanje kontaktne senzibilizacije je bila statistički značajno viša kod osoba sa hroničnom venskom insuficijencijom. Osobe sa hroničnom venskom insuficijencijom su imale 2,45 puta viši rizik za nastajanje kontaktne senzibilizacije na dva i više alergena, a 3,69 puta viši rizik za nastajanje kontaktne senzibilizacije na pet i više alergena u odnosu na one bez hronične venske insuficijencije. Učestalost kontaktne senzibilizacije je u pozitivnoj korelaciji sa dužinom trajanja bolesti.Development of allergic contact dermatitis as a complication of treatment of chronic venous insufficiency is well known. The aim of this study was to determine the incidence of eczematous contact sensitization in patients with chronic venous insufficiency, possible risks for the development of contact sensitization, and the correlation between disease duration and contact sensitization. The working hypothesis was that the incidence of contact sensitivity is significantly higher in individuals with chronic venous insufficiency than in those without chronic venous insufficiency, that they are at greater risk of developing contact sensitization, and that there is a positive correlation between the incidence of contact sensitization and the disease duration. The study included 266 patients. They were divided into two groups: the study group included patients with chronic venous insufficiency referred for allergy testing due to suspected contact dermatitis, and the control group included patients without chronic venous insufficiency patch tested for suspected contact dermatitis. The severity of chronic venous insufficiency was assessed by CEAP classification. Thereafter, each patient underwent patch testing. The research has shown that the incidence of contact sensitization among patients with chronic venous insufficiency was 49.3%. In these patients, the incidence of contact sensitization to the European standard battery of allergens was 31.55%; to the battery specific for chronic venous insufficiency it was 28.45%; to commercially available batteries of allergens of ubiquitous plants from the Compositae family it was 6.69%; and to originally prepared extracts of ubiquitous plants found in Vojvodina it was 6.11%. There was no statistically significant difference in relation to sex. The prevalence of contact sensitization in patients with chronic venous insufficiency was not significantly different from those without chronic venous insufficiency. Positive patch test reaction rates, confirming the existence of contact sensitization, were significantly higher in patients with chronic venous insufficiency. Patients with chronic venous insufficiency had a 2.45-fold higher risk for developing contact sensitization to two or more allergens, and a 3.69-fold higher risk for developing contact sensitization to five or more allergens compared to those without chronic venous insufficiency. There was a positive correlation between the incidence of contact sensitization and the duration of the disease.
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Cortial, Angèle. "Nouvelles applications des nanoparticules organiques : de la vectorisation d'un mélange d'actifs à travers la peau jusqu'au développement d'un test diagnostique in vitro de l'allergie aux parfums." Thesis, Lyon 1, 2015. http://www.theses.fr/2015LYO10018/document.
Abstract:
Les nanoparticules (NPs) organiques représentent un outil majeur d'innovation en dermatologie. L'objectif de cette thèse a été de développer et d'optimiser des procédés d'encapsulation d'un mélange de molécules odorantes appelé fragrance mix I (FMI) dans des nanoparticules (NPs) de différentes natures: NPs polymères (poly-ε-caprolactone, PCL), ou NPs lipidiques solides (SLNs) (à base de vaseline, beurre de karité, cire de candelilla, triglycérides C10-18, ou palmitate de cétyle). Ces nouveaux systèmes ont alors été évalués pour la vectorisation de ce mélange à travers un explant de peau de porc, afin de modéliser la distribution des molécules composant le FMI dans les différentes assises cutanées. En parallèle, elles ont également été appliquées en tant que promoteurs de solubilisation du FMI pour le développement d'un nouveau test de diagnostic in vitro de l'allergie aux parfums. Nos résultats montrent que: (i) les NPs polymères, principalement anioniques, sont les plus adaptées pour promouvoir la pénétration transépidermique du FMI. Au contraire, les SLNs s'agglomèrent dans le stratum corneum, conduisant à une accumulation du FMI dans cette assise ; (ii) qu'au-delà du type de vecteur utilisé, la pénétration des molécules du FMI dans les couches les plus profondes de la peau dépend de leur coefficient de partage intrinsèque ; (iii) que les nanoparticules de PCL augmentent significativement la solubilisation du FMI dans les milieux de culture conventionnels et permettent ainsi une réactivation robuste des lymphocytes T spécifiques circulant chez des patients présentant une allergie au parfums. L'ensemble de ces résultats confirme donc tout le potentiel des NPs organiques pour le développement de futures stratégies de délivrance ciblée de plusieurs actifs dans les différents compartiments cutanés. Ces nouveaux vecteurs offrent en outre une alternative prometteuse pour améliorer le diagnostic de l'eczéma de contact induit par les parfums et plus généralement par des allergènes hydrophobesThe aim of this work was to develop and optimize methods for fragrance mix I (FMI) encapsulation into nanoparticles (NPs) of two types of nanoparticles (NPs) : polymeric NPs (poly-ε-caprolactone, PCL) and solid lipid NPs (SLNs) (prepared with petrolatum, shea butter, candelilla wax, C10-18 triglycerides, or cetyl palmitate). Then, these new NPss were evaluated as vectors through a pig skin to analyze the distribution of the FMI molecules in the different skin layers. In parallel, NPs have also been applied as solubilizers for the development of a new in vitro test for the diagnosis of fragrance allergy. Our results show that (i) NPs polymers, mainly anionic NPs, are the most suitable vectors to promote trans-epidermal penetration of fragrance. On the contrary, SLNs were found in the stratum corneum, leading to an accumulation of fragrance in this layer; (ii) whatever the type of NPs, the penetration of the FMI molecules in the deeper layers of the skin depends on their intrinsic partition coefficient; (iii) PCL-NPs significantly increase the FMI solubilization in conventional culture media and, allowing a robust reactivation of circulating specific T cells in patients with allergy to fragrances. All of these results confirm the potential of organic NPs for the development of future strategies (for the skin delivery of several actives in the different skin layers). These new vectors further offer a promising alternative to improve the diagnosis of contact dermatitis induced by fragrances and more generally by hydrophobic allergens
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Del, Bufalo Aurelia. "Effets des sensibilisants sur la synthèse de la prostaglandine E2 : Mécanismes et intérêt dans la prédiction de l’allergie de contact." Thesis, Paris, AgroParisTech, 2012. http://www.theses.fr/2012AGPT0003/document.
Abstract:
Les sensibilisants de contact sont des molécules réactives électrophiles qui ont la capacité de modifier des protéines de la peau pour former un antigène. Au delà de ce mécanisme d'hapténisation, le signal de danger induit par les sensibilisants conduisant à l'activation des cellules dendritiques (DC) est un élément déterminant dans l'induction de cellules T spécifiques de l'haptène. Dans le contexte du 7ième amendement à la directive cosmétique européenne, la mise en place d'une batterie de tests in vitro permettant de prédire le potentiel sensibilisant de molécules est indispensable pour l'industrie cosmétique. Tandis que la plupart des études in vitro étudient les signaux de danger induits par les sensibilisants dans des modèles homéostasiques, nous nous sommes intéressés à l'effet des sensibilisants sur la mise en place d'une réponse inflammatoire. Lorsque la lignée U937 est différenciée avec du PMA et stimulée avec du LPS, les facteurs de transcription NF-κB et Nrf2 sont activés et l'acide arachidonique (AA) est métabolisé au travers de la cascade cPLA2 / COX-2. L'ensemble de ces voies activées conduit à la production par les U937 d'un grand nombre de médiateurs inflammatoires (IL-1β, TNF-α, IL-6, IL-10, IL-8, PGE2, PGD2, TxB2). Dans ce modèle, nous avons analysé l'effet de 6 sensibilisants de potentiels variés (DNCB, PPD, HQ, PG, CIN, EUG) et montré que de façon inattendue, tous les sensibilisants étudiés diminuent significativement et de façon spécifique la production de tous les prostanoïdes et en particulier de PGE2 induite par PMA/LPS. Nous avons de plus démontré que selon les sensibilisants, les cibles de cette inhibition au sein de la cascade métabolique de l'AA diffèrent, même si elles se focalisent la plupart du temps (sauf pour le DNCB) sur l'enzyme COX-2 (inhibition de son expression et/ou de son activité). Pour le DNCB, le mécanisme d'inhibition semble plutôt impliquer sa capacité à réagir fortement avec les groupements résidus thiols, ce qui se traduit en particulier par la déplétion du GSH intracellulaire et engendrerait l'inhibition des synthases dépendantes du GSH pour leurs activités. En parallèle de cette étude mécanistique, nous avons appréhendé la problématique du point de vue statistique et vérifié sur un set plus important et diversifié de molécules (160 molécules) que le paramètre « inhibition de PGE2 » pouvait être un bon test de prédiction de l'HSRC. L'étude statistique a permis de déterminer le modèle prédictif du test PGE2 et de mettre en évidence de bonnes performances (78%) par rapport aux prédictions du LLNA. Au-delà, une certaine complémentarité du test PGE2 avec d'autres tests in vitro (MUSST, Nrf2-HTS) a pu être mise en évidence. En conclusion, au travers de cette étude, nous avons pu mettre en évidence de nouvelles propriétés biochimiques des sensibilisants. Même si la signification biologique de la diminution de PGE2 par les sensibilisants de contact demeure complexe d'interprétation, ce paramètre a permis le développement d'un test qui prédit avec de bonnes performances le caractère sensibilisant de molécules et dont la position au sein d'une batterie prédictive d'évaluation de l'allergie de contact reste à être préciséeContact sensitizers are defined as reactive molecules (electrophilic) which have the ability to modify skin proteins to form an antigen (hapten). In addition to the haptenation mechanism, danger signals, leading to the activation of dendritic cells, are described to be crucial for the effective induction of an hapten-specific T cell immune response. In the context of the 7th amendment to the Cosmetic Directive, the cosmetic industry is concerned by the challenge of finding non-animal approaches to assess the sensitizing potential of chemicals. While danger signals induced by sensitizers in steady-state conditions have already been analyzed, we chose to investigate the impact of sensitizers on the course of an inflammatory response. For this purpose we used the U937 cell line differentiated with PMA and activated with LPS. In these conditions, cells produce a large amount of inflammatory mediators (IL-β, TNF-α, IL-6, IL-10, IL-8, PGE2, PGD2, TxB2) through the activation of pathways leading to the activation of the transcription factors NF-κB and Nrf2 and through AA metabolism by the cPLA2/COX-2 cascade. Interestingly, we showed that 6 contact sensitizers with various potential (DNCB, PPD, HQ, PG, CIN, EUG) significally and specifically decrease the production of prostanoïds and in particular of PGE2 induced by PMA/LPS. We further demonstrated that there is no unique inhibition profile of the sensitizers even if the majority (except for DNCB) of the effects applies on COX-2 (i.e. inhibition of the expression and/or activity). For DNCB, inhibition mechanism appears to be dependant of its capacity to react with thiols residues and in particular to deplete intracellular glutathione possibly leading to the inactivation of the PG-synthases. In parallel, we assess a statistical analysis on 160 molecules that allow us to define the test parameters (a molecule is a sensitizer if the PGE2 inhibition at 24h is more than 60%) and to calculate the test performance toward LLNA (78%). Moreover we demonstrated that the PGE2 test could be complementary to other already existing in vitro tests like MUSST or Nrf2-HTS. In summary, we add here a new insight into the multiple biochemical effects described so far for sensitizers. Even if the underlying biological relevance remains unclear, the parameter “PGE2 inhibition” is good test for skin sensitization evaluation. Further studies will precise how this parameter could be implemented into an alternative testing strategy for the evaluation of skin sensitization
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Krakovková, Lenka. "Stanovení alergenních a potenciálně alergenních kovů v kosmetických přípravcích." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2010. http://www.nusl.cz/ntk/nusl-216609.
Abstract:
The aim of the diploma thesis was to provide an overview of the prevalence of allergenic and potentially allergenic metals in eye shadows. The diploma thesis gives an overview of the legislation on cosmetics and description of the types of allergenic reactions caused by allergenic and potential allergenic metals in eye shadows. Listed below are the preparation methods of the samples for analysis and the list of the methods by which can the selected metals be analyzed. The experimental part of the diploma thesis deals with an analysis of selected allergenic and potentially allergenic metals in eye shadows. In the experimental part of diploma thesis method of sample preparation for analysis of eye shadows and a method of analysis of sample of eye shadows by ICP-MS have been optimized. Monitored analytes were selected metals. Altogether 6 samples of eye shadows from different manufacturers were chosen. Measured results have been statistically processed, confronted with the applicable legislation and assessed in terms of possible allergic reactions.
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Kuresepi, Salen. "Alternative mechanisms in skin allergy processes : contribution of radical reactions from the molecule to the tissue." Thesis, Strasbourg, 2018. http://www.theses.fr/2018STRAF010/document.
Abstract:
L’allergie de contact est une pathologie touchant de 15 à 20 % de la population occidentale. A l’heure actuelle il n’existe aucun traitement, la seule façon efficace de prévention étant l’éviction totale des allergènes. Les tests de sensibilisation de nouvelles molécules avant leur mise sur le marché ont été réalisés sur l’animal jusqu’à l’interdiction dans le 7ème amendement à la directive Européenne concernant l’industrie cosmétique. Dans ce contexte il est primordial de développer des méthodes alternatives. Ce travail de thèse propose d’analyser la problématique de l’allergie de contact en allant de la molécule au tissu pour les allergènes réagissant par voie radicalaire :In chemico : étude de la réactivité des hydroperoxydes allyliques vis-à-vis des acides aminés par la RMNIn situ : études de radicaux issus de ces composés sur des épidermes humains reconstitués par RPEIn cellulo : étude du stress oxydant sur les cellules dendritiques et la voie de signalisation Keap1/Nrf2/AREAllergic contact dermatitis is a pathology affecting 15 to 20% of the Western population. Until now no treatment exists, the prevention is the eviction of allergens. In the past, tests concerning new molecules for the market were tested on animals until the prohibition in the 7th amendment of the European directive concerning the cosmetics industry. In this context it is essential to develop alternative methods to assess the allergenic potential of chemicals.This manuscript proposes to analyze the problem of the allergic contact dermatitis from the molecule to the tissue for allergens reacting through radical mechanisms:In chemico: study of the reactivity profile of allylic hydroperoxides toward amino acids by NMRIn situ: radical intermediates formation on reconstructed human epidermis from allylic hydroperoxides by EPR In cellulo: study of the oxidative stress from allylic hydroperoxides on dendritic cells trough the Keap1/Nrf2/ARE sensor pathway
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Gamradt, Pia. "Tissue-resident memory T cells in eczema : contribution and protective regulatory mechanisms." Thesis, Lyon, 2017. http://www.theses.fr/2017LYSE1306/document.
Abstract:
Les eczémas [eczéma allergique de contact (EAC) et l'eczéma atopique (EA)] sont des dermatoses inflammatoires fréquentes des pays industrialisés. Elles sont induites suite au recrutement et à l'activation dans la peau de lymphocytes T spécifiques d'allergènes, qui sont présents dans notre environnement, et qui sont habituellement très bien tolérés par la majoritédes individus exposés. Ce travail de thèse porte sur un aspect novateur de la physiopathologie des eczémas, à savoir : la contribution des lymphocytes T mémoires résidants (LTrm) dans la peau à la chronicité et à la sévérité de ces maladies.Capitalisant sur des modèles précliniques pertinents ainsi que sur des échantillons cliniques prélevés chez les patients, ce travail a permis d'acquérir de nouvelles connaissances : (i) de nombreux LTrm CD8+ spécifiques colonisent les lésions d'eczéma (ii) ils s'accumulent avecla persistance de l'allergène dans la peau, (iii) ils jouent un rôle majeur dans les récidives de la maladie, mais (iv) ils expriment à leur surface divers récepteurs inhibiteurs, tels que PD-1 ou TIM-3, qui empêchent la survenue de réponses allergiques excessives.Ces travaux apportent donc des informations majeures sur la nature unique des LTrm CD8+ spécifiques d'allergènes et des mécanismes qui contrôlent leur réactivation, afin de préserver l'intégrité de la peau et la survenue de réactions chroniques sévères. Le développement des nouvelles stratégies thérapeutiques ciblant la réactivation des LTrm via leurs récepteursinhibiteurs pourrait permettre de restaurer la tolérance chez les individus allergiquesAllergic contact dermatitis (ACD) and atopic dermatitis (AD), also referred to contact or atopic eczema, are frequent skin inflammatory diseases with increasing prevalence and high socioeconomic impact in Western countries. Eczemas are the prototype of skin delayed-type hypersensitivity reactions. Skin lesions are induced by the recruitment and activation in the skin of effector/memory T cells specific for environmental antigens that are innocuous to healthy non-allergic individuals.The aim of this work was to better understand the pathophysiology of eczemas by a comprehensive analysis of the contribution of skin resident memory T cells (Trm) to the chronicity and severity of these diseases.Capitalizing on relevant preclinical eczema models and on clinical samples collected from allergic patients, this work showed that: (i) numerous allergen-specific CD8+Trm colonize the eczema lesion, (ii) they accumulate in the epidermis in response to the long-term persistence of the allergen in the skin, (iii) they are instrumental for the recurrence of eczema, but (iv) theyexpress several inhibitory check point receptors (ICRs, such as PD-1, TIM-3) at their surface, which keep them in check to prevent the development of severe immunopathology.Thus, our work provides important information for considering the unique nature of hapteninduced CD8+ Trm and the mechanisms that prevent their unwanted reactivation and subsequent development of chronic or severe skin allergy. The development of therapeutic strategies targeting the reactivation of skin Trm in situ via their ICRs should open new avenues to restore tolerance in allergic individuals
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Plantamura, Emilie. "Rôle de la voie des hélicases de type RIG dans la régulation de l'homéostasie du microbiote intestinal et des réponses inflammatoires « stériles »." Thesis, Lyon 1, 2014. http://www.theses.fr/2014LYO10251.
Abstract:
La voie des RLR (RIG-I like Receptors) joue un rôle essentiel dans la détection des virus à ARN, déclenchant une réponse immunitaire antivirale grâce au recrutement de la protéine adaptatrice mitochondriale MAVS (Mitochondrial AntiViral Signaling protein). Nous avons mis en évidence que les souris déficientes pour la protéine MAVS (MAVS KO) présentaient un phénotype proallergénique dans un modèle d'inflammation stérile d'hypersensibilité retardée de contact (HSRC) qui reproduit la dermatite allergique de contact (DAC) chez l'homme. Nous avons caractérisé le système immunitaire des souris MAVS KO en condition d'équilibre et durant la réponse d'HSRC. Nous avons identifié un rôle du microbiote intestinal des souris MAVS KO dans l'exacerbation de réponse d'HSRC et mis en évidence une dysbiose du microbiote bactérien. Nous avons démontré que la dysbiose était responsable du phénotype inflammatoire observé, phénotype transmissible à des souris sauvages par des expériences de cohébergement et de transplantation fécale. Cette dysbiose induit une augmentation de la perméabilité intestinale chez les souris MAVS KO lors de la réponse d'HSRC, aboutissant à une translocation bactérienne dans les organes lymphoïdes et à la modulation des réponses immunitaires à l'origine de l'exacerbation de réponse d'hypersensibilité. La 2ème partie de ma thèse vise à étudier les conséquences de la déficience en MAVS sur le métabolisme glucidique. Nos expériences ont démontré que les souris MAVS KO développaient une surcharge pondérale et une insulino-résistance sous régime riche en lipides et sucrose, dépendants de la dysbiose intestinale. Au niveau cellulaire, une altération des interactions aux points de contact entre la mitochondrie et le réticulum endoplasmique a été observée. Nos résultats permettent d'envisager le développement de nouvelles approches thérapeutiques des pathologies allergiques et métaboliques humaines par la modulation du microbiote intestinalRIG-I like receptors (RLRs) play a major role in response to cytosolic viral RNAs by initiating an antiviral immune response through the recruitment of the mitochondrial adaptor protein MAVS (Mitochondrial AntiViral Signaling protein). We showed that MAVS-deficient mice developed an exacerbated response in a sterile inflammatory model of Contact Hypersensitivity (CHS), that reproduces the pathophysiology of allergic contact dermatitis (ACD) in human. We characterized the immune system of MAVS KO mice at steady state and during CHS response. We found that MAVS deficiency leads to changes in the gut bacterial composition suggesting an unexpected role of the RLR pathway in the regulation of intestinal homeostasis. We demonstrated that intestinal dysbiosis is responsible for the increased CHS response, and showed that the inflammatory phenotype of MAVS KO mice can be transferred to WT mice by cohousing and fecal transplantation. We demonstrated that the dysbiotic gut microbiota exerts its effect due to an increased intestinal permeability during DTH sensitization. The ensuing bacterial translocation within lymphoid organs enhances characteristic cytokines production that increases CHS response. The 2nd part of my thesis aimed to study the consequences of MAVS deficiency on glucose metabolism. Our experiments showed that MAVS KO mice exhibit disorders of glucose homeostasis during high fat diet (HFD) associated with the development of overweight and insulin resistance. We also observed alterations of MAM (Mitochondria-associated endoplasmic reticulum membranes), contact poins between mitochondria and endoplasmic reticulum. Recent preliminary data suggested that the metabolic disorders associated with MAVS deficiency are due to intestinal dysbiosis. Our results highlight a new role for the RLR pathway and allow to consider the development of new therapeutic approaches to human allergic and metabolic diseases by modulation of the intestinal microbiota
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Aleksandra, Petrović. "Uticaj pola, težine i dužine trajanja oboljenja na kontaktnu senzibilizaciju kod obolelih od vulgarne psorijaze." Phd thesis, Univerzitet u Novom Sadu, Medicinski fakultet u Novom Sadu, 2015. https://www.cris.uns.ac.rs/record.jsf?recordId=90404&source=NDLTD&language=en.
Abstract:
Kontaktna senzibilizacija, kao stanje specifične reaktivnosti kože, može biti egzogeni pokretač psorijaze. Rezultat je interakcije endogenih i egzogenih činioca. Jedan od najznačajnijih endogenih faktora kome se pripisuje uloga faktora rizika jeste pol. Cilj istraživanja bio je da se kod obolelih od psorijaze utvrdi učestalost i distribucija kontaktne senzibilizacije u odnosu na pol, razlike u distribuciji kontaktne senzibilizacije po polu između osoba obolelih od psorijaze i osoba pod sumnjom na postojanje kontaktnog alergijskog dermatitisa, povezanost kontaktne senzibilizacije i težine kliničkog nalaza i povezanost kontaktne senzibilizacije i dužine trajanja oboljenja. Hipoteza istraživanja polazila je od pretpostavke da je kontaktni alergijski dermatitis redak kod obolelih od psorijaze, jer hronična inflamacija u koži smanjuje njenu sposobnost specifične senzibilizacije a da je veća učestalost kontaktne senzibilizacije kod osoba ženskog pola rezultat egzogenog faktora tj.ekspozicije, kao i da je učestalost kontaktne senzibilizacije u pozitivnoj korelaciji sa težinom i dužinom trajanja oboljenja. Istaživanje je sprovedeno kod 176 ispitanika koji su bili podeljeni u dve grupe. Eksperimentalnu grupu su činili oboleli od psorijaze, a kontrolnu grupu ispitanici upućeni na alergološko testiranje pod sumnjom na postojanje kontaktnog alergijskog dermatitisa. Obolelima od psorijaze je ocenjivana težine oboljenja PASI skorom, a svi ispitanici bili su alergološki testirani epikutanim -pač testom. Pozitivni rezultati alergološkog testiranja su analizirani, u cilju utvrđivanja kliničke relevantnosti istih. Ispitanicima u grupi obolelih od psorijaze je testom skarifikacije određivano prisustvo ili odsustvo Koebnerovog izomorfnog podražajnog fenomena. Istraživanjem je utvrđeno, da se kontaktno reagovanje kod obolelih od psorijaze na najmanje jedan standardni alergen nije statistički značajno razlikovalo od reagovanja osoba kod kojih je postavljena sumnja na postojanje kontaktnog alergijskog dermatitisa, ali je senzitivnost izražena kroz prosečan broj pozitivnih testova po jednom ispitaniku bila statistički značajno niža kod obolelih od psorijaze. Poređenjem kontaktnog reagovanja muškaraca i žena nisu utvrđene značajna razlike u reagovanju u odnosu na pol. Težina oboljenja nije uticala na učestalost kontaktne senzibilizacije kod obolelih od psorijaze, ali je učestalost kontaktne senzibilizacije bila u pozitivnoj korelaciji sa dužinom trajanja bolesti. Niža stopa kontaktnog reagovanja utvrđena je kod osoba obolelih od psorijaze koji su imali pozitivan Koebnerov fenomen u trenutku ispitivanja.Contact sensitization as a state of specific skin reactivity may provoke psoriasis resulting from an interaction between extrinsic and intrinsic factors. One of the most significant factors characterized, as a risk factor, is the sex. The aim of this study was to determinate the frequency and distribution of contact sensitization in patients with psoriasis with respect to their sex, as well as the differences in the distribution of contact sensitization in both sexes, namely with patients with psoriasis and patients suspected to allergic contact dermatitis. Consequently, appropriate attention was paid to the correlation between contact sensitization and disease severity, and between contact sensitization and disease duration. Hypothesis were based on the assumption that allergic contact dermatitis is rare in patients with psoriasis, as a chronic inflammation of the skin reduces its ability specific sensitization, as well as that the higher frequency of contact sensitization in females represent a result of exogenous factors, i.e. exposition, and finally that there is a positive correlation between the incidence of contact sensitization and the disease severity, and contact sensitization and the disease duration. The study included 176 patients. They were divided into two groups: the study group included patients with psoriasis, while the control group included patients referred for allergy testing, since they were suspected to allergic contact dermatitis. The severity of psoriasis was evaluated by PASI score. Thereafter, each patient underwent patch testing. The positive results of patch tests were evaluated with the aim to define their clinical relevancy. Subjects from the group of psoriatic patients passed scarification test carried out to indicate the presence or absence of Köebner isomorphic phenomenon. This research led us to the conclusion that the positive reaction of psoriatic patients to at least one standard allergen did not indicate a statistically significant different reaction when compared to the reaction of patients suspected to allergic contact dermatitis. From the other side, the sensitivity expressed through the average number of positive tests per one tested patients was significantly lower in patients with psoriasis. Comparison of the contact response of men and women showed no significant differences in response with respect to their sex. The disease severity did not influence the frequency of contact sensitization in patients with psoriasis. At the same time, the frequency of contact sensitization stood in a positive correlation with the duration of disease. The lower rate of contact sensitization was found in patients with psoriasis who have had a positive Köebner phenomenon at the time of testing.
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Gomez, de Agüero Tamargo Mercedes. "Rôles des cellules de Langerhans épidermiques dans l'induction et la rupture de la tolérance immunitaire aux allergènes cutanés." Thesis, Lyon 1, 2011. http://www.theses.fr/2011LYO10312.
Abstract:
La tolérance périphérique vis-à-vis de molécules potentiellement allergéniques en contact avec la peau joue un rôle essentiel pour prévenir le développement de l’eczéma allergique de contact (EAC). Au cours de ma thèse, j'ai contribué à l'identification des mécanismes et des acteurs responsables de l'induction de la tolérance par voie cutanée et à préciser le rôle respectif des sous-populations de cellules dendritiques (DC) cutanées dans la rupture de la tolérance et l'induction de lymphocytes T (LT) CD8+ initiant l'EAC. A l'aide d'un modèle murin d'induction de tolérance aux haptènes, j’ai pu montrer que les cellules de Langerhans (LC) épidermiques sont les cellules clés pour induire la tolérance cutanée et empêcher le développement d'un EAC médié par les LT CD8+. En effet, suite à l’application épicutanée d’un allergène/haptène faible, le DNTB, les LC migrent de la peau aux ganglions lymphatiques pour présenter l’antigène aux LT CD8+. Des expériences de déplétion in vivo et de transfert adoptif montrent que les LC sont responsables de la suppression de l’EAC en prévenant la différentiation des LT CD8+ spécifiques de l'allergène en cellules T cytotoxiques via deux mécanismes complémentaires: i) l’anergie/délétion des LT CD8+ et ii) l'activation de LT régulateurs Foxp3+ exprimant ICOS. Après avoir identifié des conditions d'immunisation conduisant au développement d'un EAC au DNTB, j'ai montré que la rupture de tolérance à ce type d'allergène est associée à i) à des modifications phénotypiques des LC épidermiques, ii) au recrutement rapide de monocytes inflammatoires Gr1+ dans la peau et iii) à une capacité équivalente des LC et des DC dermiques Langerin- à présenter l'allergène aux LT CD8+ dans les ganglions. Dans cette situation, les LC jouent un rôle pro-inflammatoire puisque leur déplétion réduit de manière dramatique l'induction de LT CD8+ effecteurs et l'EAC. Ces résultats indiquent que les LC jouent un rôle essentiel à la fois dans la prévention et dans l’induction de l’EAC, et que leur fonction tolérogène ou stimulatrice est vraisemblablement conditionnée par le microenvironnement cutané lors de la pénétration de l’allergèneInduction of peripheral tolerance to potentially allergenic molecules in contact with the skin is essential to prevent the development of allergic contact dermatitis (ACD). During my PhD, I contributed to the identification of the mechanisms and actors responsible for the induction of skin tolerance and clarified the respective roles of dendritic cell (DC) subsets in the breakdown of skin tolerance leading to the priming of cytotoxic CD8+ T cells and developpement of ACD. Using a mouse model of cutaneous tolerance to a model weak allergen, we show that epidermal Langerhans cells (LC) are essential to induce CD8+ T cell tolerance and prevent the development of ACD. Indeed, following the epicutaneous delivery of the weak allergen/hapten DNTB, LC were found to migrate from skin to draining lymph nodes to present the allergen to CD8+ T cells. Depletion and adoptive transfer experiments revealed that LC protect from development of ACD by preventing the priming of allergenspecific cytotoxic CD8+ T cells via two complementary mechanisms: i) anergy/deletion of allergen-specific CD8+ T cells and ii) activation of highly suppressive Foxp3+ regulatory T cells expressing ICOS. We identified DNTB skin delivery conditions that allow for CD8+ T cell priming and initiation of ACD. Breakdown of tolerance to this weak allergen was associated with i) phenotypic modifications of epidermal LC, ii) recruitment of inflammatory monocytes to the skin and iii) allergen presentation to CD8+ T cells by both LC and dermal Langerin- DC. In addition, LC are involved in tolerance breakdown as their depletion prior to skin immunization abrogated induction of CD8+ effector cells and ACD. These results demonstrate that LC are essential for both the induction of skin tolerance to weak skin allergens and for the induction of ACD, and suggest that their tolerogenic versus immuno-stimulatory function is likely dictated by signals from the skin microenvironment after penetration of the allergen
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Bourayne, Marie de. "Rôle de la CK2 dans l’activation de la réponse immunitaire induite par les molécules allergisantes et son lien avec Nrf2." Thesis, Paris 11, 2015. http://www.theses.fr/2015PA114823/document.
Abstract:
L’eczéma allergique de contact (EAC) est une réaction inflammatoire aiguë médiée par les lymphocytes T (LT), survenant suite à l’exposition répétée de la peau avec une molécule allergisante présente dans l’environnement quotidien ou professionnel. Les molécules allergisantes sont des composés de faible poids moléculaire, appelés haptènes, qui activent les cellules dendritiques (DC). Les DC jouent un rôle essentiel dans la mise en place d’un EAC : elles acquièrent un phénotype mature, contrôlé par la voie des MAPK et la voie NF-B, leur permettant de présenter l’haptène aux LT afin d’initier ainsi une réponse immunitaire spécifique.Nous avons identifié au sein de la DC une nouvelle kinase, la protéine kinase CK2, indispensable à l’acquisition d’un phénotype mature et à la sécrétion de cytokines pro-inflammatoires clés dans l’orientation d’une réponse immunitaire. L’activité de la CK2 dans la DC est nécessaire à la génération d’une réponse Th1 en contrôlant la sécrétion d’IFN par les LT, et maintient une réponse Th17 préexistante. De plus, la CK2 permet à la DC de contrôler l’induction d’une réponse Th2 spontanée. Par ailleurs, la CK2 contrôle l’expression des gènes cibles de Nrf2, un facteur de transcription majeur dans la lutte contre le stress chimique induit par les haptènes. L’activation de Nrf2 met en jeu de nombreuses voies de signalisation, et nous avons mis en évidence c-Jun, facteur de transcription activé par les molécules allergisantes, comme un potentiel partenaire transcriptionnel de Nrf2Allergic contact dermatitis represents a severe health problem with increasing worldwide prevalence. It is a T cell-mediated inflammatory skin disease caused by chemicals present in daily or professional environment. Contact sensitizers are low molecular weight compounds termed haptens. These molecules are known to induce an up-regulation of phenotypic markers and cytokine secretion in dendritic cells (DCs), professional antigen-presenting cells, leading to the generation of effector T lymphocytes (LT).We identified a new kinase, termed CK2 (formerly casein kinase 2), as a key kinase in DCs in the acquisition of a mature phenotype and in the production of pro-inflammatory cytokines, involved in T cell polarization in response to contact sensitizers. CK2 activity in DC is necessary to induce a Th1 polarization by controlling the secretion of IFN- by LT, and maintains a pre-existing Th17 response. Moreover, CK2 in DC negatively controls a spontaneous Th2 response.Finally, CK2 controls the expression of Nrf2 target genes mRNA. Nrf2 is a protective transcription factor playing a major role in detoxification, oxidative stress and allergic inflammation generated by contact sensitizers. Nrf2 activation involves different kinases and we highlight that c-Jun could be bound to Nrf2 to generate an active transcriptional complex in response to chemicals
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Brecheisen, Mariken, and Julia Persson. "Oral Lichenoid Lesions: Differences in expression of TLR4 and TLR9 in Oral Lichen Planus and amalgam induced Oral Lichenoid Lesions." Thesis, Malmö högskola, Odontologiska fakulteten (OD), 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:mau:diva-19936.
Abstract:
Oral lichen planus (OLP) är en idiopatisk kronisk inflammatorisk sjukdom som drabbar munslemhinnan hos ca 2 % av den svenska befolkningen. Amalgamfyllningar kan framkalla lichenoida kontaktlesioner (cOLL), som kliniskt kan vara svåra att särskilja från OLP. Det är dessutom inte möjligt att skilja mellan OLP och cOLL histologiskt. Det är viktigt att kunna särskilja OLP och cOLL då behandlingen av dem skiljer sig.Toll-like receptorer (TLR) finns på flera av kroppens celler. De är en del av det medfödda immunförsvaret men de har också kopplats till autoimmuna sjuksomar. En ökad förekomst av TLR i skivepitel har påvisats vid OLP.Syftet med denna studie är att undersöka variationer i uttrycket av TLR4 och TLR9 i OLP och cOLL. Vår hypotes är att en histologisk skillnad i OLP och cOLL ska kunna observeras p.g.a. skillnader i patogenesen mellan OLP och cOLL.Metod: Vävnadsprov med histologiskt verifierad lichenoid reaktion valdes från Biobanken, Oral Patologi, Malmö från patienter med de kliniskt ställda diagnoserna OLP (10) och cOLL (12). TLR4 och TLR9 identifierades med hjälp av immunhistokemisk färgning varefter reaktionens lokalisation och intensitet jämfördes mellan de två grupperna.Resultat: En signifikant skillnad observerades i infärgningen av TLR4 hos fibroblaster, lymfocyter och makrofager, där TLR4 var mer positiv i cOLL. Uttrycket av TLR9 hos lymfocyter var starkare vid OLP än cOLL.Slutsats: Våra resultat visade att det finns en skillnad i uttrycket av TLR4 och TLR9 i cOLL och OLP. Resultaten bekräftar att OLP och cOLL har olika patogenes, men ytterligare studier behövs för att klargöra hur.Oral lichen planus (OLP) is an idiopathic chronic inflammatory disease that affects the oral mucosa in approximately 2% of the Swedish population. Amalgam fillings may induce contact oral lichenoid lesions (cOLL) that can be difficult to clinically distinguish from OLP. It is not possible to histologically distinguish between OLP and cOLL. As their treatments differ, the correct diagnosis is vital.Toll-like receptors (TLR) are expressed by most of the body's cells and are part of the innate immune system, however they have also been linked to certain autoimmune diseases. OLP exhibits an increased amount of TLR in the epithelium.The purpose of this study is to investigate the variations in the expression of TLR4 and TLR9 in OLP and cOLL. Our hypothesis is that a histological difference in OLP and cOLL can be observed due to TLRs different roles in maintaining the immune response.Method: Tissue samples with histologically confirmed lichenoid reactions were chosen from Biobank, Oral Pathology, Malmö, from patients with the clinical diagnosis OLP (10 subjects) and cOLL (12 subjects). TLR4 and TLR9 were identified by immunohistochemical staining and compared between the two groups.Results: A significant difference was observed in TLR4 staining of fibroblasts, lymphocytes and macrophages where the antibody was less expressive in OLP. In TLR9 staining lymphocytes were stronger expressed in OLP compared to cOLL.Conclusion: Our results showed that there was a difference in the expression of TLR4 and TLR9 in cOLL and OLP, this could be a result of OLP being an autoimmune disorder. Further studies on this subject are recommended on this subject.
34
Sahli, Fatma. "Les allergènes cutanés réagissant au travers de processus radicalaires : de la molécule au tissu." Thesis, Strasbourg, 2019. http://www.theses.fr/2019STRAF023.
Abstract:
L’allergie cutanée représente un problème majeur de santé publique. La compréhension de tous les mécanismes conduisant à des réactions allergiques est capitale afin d’évaluer le potentiel de sensibilisation des molécules à risques. Les hydroperoxydes dérivés de l’autoxydation de terpènes utilisés dans les produits cosmétiques sont à l’origine de sensibilisations cutanées. L’objectif a été d’étudier l’intervention de mécanismes radicalaires dans la formation d’entités antigéniques de ces composés. Une approche intégrée de trois actions a été proposée : - "in chemico" : études de réactivité vis-à-vis des acides aminés afin d’établir un profil de réactivité complet suscitant des réactions radicalaires; - "in situ" : étude de la formation et comportement des intermédiaires radicalaires formés dans un modèle 3D d’épiderme humain reconstitué ; - "in cellulo" : étude de leur capacité à activer le système immunitaire inné, à savoir les cellules dendritiques dans leur contexte naturel kératinocytaireSkin allergy is a major public health problem. It is the most important immunotoxic reaction in humans. Understanding all mechanisms leading to allergic-inflammatory reactions is essential in order to evaluate the sensitization potential of molecules for risk assessment purposes. Hydroperoxides derived from autoxidation of odorant terpenes used in cosmetics cause skin sensitization. The objective was to study the involvement of radical mechanisms in the formation of the antigenic entities of these compounds. An integrated approach of three actions was proposed: - "in chemico" : reactivity studies with amino acids to establish a complete reactivity profile via radical reactions ; - "in situ" : formation and behavior of radical intermediates in a reconstructed human epidermis 3D model ; - "in cellulo" : ability to activate the innate immune system, namely dendritic cells in their natural keratinocyte context
35
Abrams, Stephanie B. "Evaluation of Veterinary Allergen Extract Content and Resultant Canine Intradermal Threshold Concentrations." The Ohio State University, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=osu1524066093298819.
36
Debeuckelaere, Camille. "Synthèse d'allergènes marqués au carbone 13 et études par RMN HRMAS de leurs interactions avec des épidermes reconstruits." Thesis, Strasbourg, 2012. http://www.theses.fr/2012STRAF050.
Abstract:
L'allergie de contact est une pathologie en constante augmentation et particulièrement répandue dans les pays industrialisés. Aucune thérapie n'existe actuellement et seule l'éviction totale de l'allergène permet d'éviter toute nouvelle réaction d'allergie. La base de l'allergie de contact est la formation d'une liaison entre l'allergène et les protéines épidermiques. C'est cette étape chimique clé qu' il est important de comprendre afin de développer de nouvelles méthodes dites« alternatives» dans le cadre de l'actuelle législation sur les cosmétiques. Le but de ce travail de thèse a été d'étudier la réactivité de différents allergènes connus face aux acides aminés et protéines d'épidermes reconstruits de type SkinEthic® par la technique RMN HRMAS dérivée de la RMN du solide. Six allergènes ont ainsi été étudiés et leur réactivité a été comparée a celle observée en solution face à une protéine modèleContact dermatitis is one of the most common health problem and highly prevalent in industrialized countries. No therapy currently exists and only the total eviction of the allergen can prevent further allergie reaction.The key molecular event in skin sensitization is the formation of a bond between the allergen and the epidermal proteins. Due to the recent legislation on cosmetic and to help avoid the inappropriate use of new allergens, the understanding of this key step has to be expanded in order to develop new alternative metbods.The aim of this PhD work is to study the reactivity of some allergens towards amino acids and proteins presents in reconstructed human epidermis like SkinEthic® using the HRMAS NMR technique. Six allergens have been studied and their reactivity was compared to that observed ln solution with a model protein
37
Laporte, Dominique. "Les allergies cosmétiques." Toulouse 3, 1995. http://www.theses.fr/1995TOU32073.
38
Nciri, Ilona. "Sulfites et dermatites de contact professionnelles." Bordeaux 2, 1999. http://www.theses.fr/1999BOR23067.
39
Al-Amri, Mohammad Abdulla. "Atopic dermatitis : the role of contact allergy in disease and disease exacerbations." Thesis, King's College London (University of London), 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.274922.
40
Eilstein, Joan. "Etude de la réactivité de dérivés d'oxydation de la 2,5-diméthyl-para-phénylènediamine vis-à-vis d'acides aminés nucléophiles." Université Louis Pasteur (Strasbourg) (1971-2008), 2006. http://www.theses.fr/2006STR13071.
41
Graffin, Florence. "L'allergie aux épices : étude effectuée sur 18 épices." Paris 5, 1996. http://www.theses.fr/1996PA05P071.
42
Rouquet, Olivier. "Allergie au latex : enquête épidémiologique en milieu hospitalier." Montpellier 1, 1997. http://www.theses.fr/1997MON11035.
43
Jossec, Florence Milpied-Homsi Brigitte. "Allergie à la lanoline et à ses dérivés: actualité à propos d'une analyse de trois ans dans un service de dermatologie /." [S.l.] : [s.n.], 2004. http://theses.univ-nantes.fr/thesemed/PHjossec.pdf.
44
Pailler, Christine. "Allergie cutanée aux constituants des emballages plastiques alimentaires et pharmaceutiques." Montpellier 1, 1993. http://www.theses.fr/1993MON11136.
45
Abenhaim, Cécile. "Allergie aux gants en latex. Dosage des protéines extractibles contenues dans les gants en latex." Paris 5, 1998. http://www.theses.fr/1998PA05P140.
46
Le, Ngoc Michel. "Les effets allergiques et toxiques du nickel." Paris 5, 1997. http://www.theses.fr/1997PA05P094.
47
Lang, Matthieu. "Évaluation du pouvoir allergisant de mélanges complexes : étude de la réactivité de molécules sensibilisantes en mélanges envers des peptides nucléophiles." Phd thesis, Université de Strasbourg, 2012. http://tel.archives-ouvertes.fr/tel-00780099.
Abstract:
L'allergie de contact est une pathologie très répandue. Il n'existe cependant aucun traitement permettant de la soigner. L'éviction de l'allergène reste à ce jour reste le seul moyen de la prévenir. De nombreux tests visant à déterminer le pouvoir allergisant des molécules ont été développés. L'un de ces tests est le Direct Peptide Reactivity Assay (DPRA), une méthode chimique visant à mimer une étape clé du mécanisme immunitaire menant à l'allergie de contact : la formation d'un complexe antigénique par réaction entre l'allergène et une protéine cutanée. Dans le cadre de la législation actuelle sur les cosmétiques, il pourrait être intéressant d'adapter ce test aux mélanges complexes. Le but de ce travail de thèse a été d'étudier la réactivité de différents mélanges d'allergènes connus vis-à-vis des trois peptides utilisés dans le DPRA et de la comparer à celle de chaque composant seul.Trois types de mélanges ont étés étudiés. Un mélange d'isothiazolinones utilisées comme conservateurs, trois mélanges binaires de trois aldéhydes utilisés comme parfums et enfin un mélange des deux responsables majeurs de l'allergie à l'absolu de mousse de chêne, un extrait utilisé en parfumerie.
48
Faltot, Dominique. "Ulcères de jambe : sensibilisation aux allergènes de contact, étude prospective." Bordeaux 2, 1990. http://www.theses.fr/1990BOR23095.
49
Plumenail, Christelle. "Oxatomide : un nouveau traitement de l'allergie cutanée." Bordeaux 2, 1992. http://www.theses.fr/1992BOR2P015.
50
Moss, Éric. "Etude in situ par RMN HRMAS sur des épidermes reconstruits du métabolisme et de la réactivité de xénobiotiques allergisants." Thesis, Strasbourg, 2015. http://www.theses.fr/2015STRAF003/document.
Abstract:
L’allergie de contact est une pathologie de la peau particulièrement répandue dans les pays industrialisés. Aucune thérapie ne permet actuellement de la soigner et seule l’éviction de l’allergène permet de la prévenir. Historiquement, l’évaluation du potentiel sensibilisant des molécules mises sur le marché a toujours été réalisée au moyen de tests sur l’animal. Cependant, le champ d’action de ces tests est aujourd’hui limité en raison de la nouvelle législation européenne sur les cosmétiques. Dans ce contexte, le développement de méthodes alternatives ne reposant pas sur l’utilisation d’animaux devient capital. L’allergie de contact repose sur une étape chimique clé : la formation d’un complexe antigénique allergène-protéine capable d’activer le système immunitaire cutané. Le but de ce travail de thèse a été d’étudier le comportement in situ d’allergènes au sein d’épidermes reconstruits de type SkinEthic®. A l’aide d’une technique d’analyse non invasive, la spectroscopie RMN HRMAS, il a été possible de suivre le devenir de différents allergènes, de leur éventuelle activation par voie métabolique, jusqu’à leur fixation sur les protéines épidermiquesContact dermatitis is a skin pathology particularly prevalent in industrialized countries. No therapy currently exists and only complete avoidance of the particular allergen can prevent an allergic reaction. Historically, the assessment of skin sensitisation potential of molecules placed on the market was always carried out by animal testing. However, the scope of this testing method is now limited by the new European cosmetics legislation. In this way, the development of alternative methods, not based on animal experimentation, become an important issue. Contact dermatitis results of a chemical key step: the formation of an antigenic complex allergen-protein complexe able to activate the cutaneous immune system. The aim of this PhD work was to study the in situ behaviour of allergens in reconstructed human epidermis (SkinEthic® model). By using an appropriate non-invasive analysis technique, HR-MAS NMR spectroscopy, it has been possible to study the mode of action of different allergens, from their possible activation through the metabolic pathway to the binding with epidermal proteins