Relevant bibliographies by topics / Fatty acyl desaturase 2

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  1. Journal articles
  2. Dissertations / Theses
  3. Book chapters
  4. Conference papers
  5. Reports

Academic literature on the topic 'Fatty acyl desaturase 2'

Author: Grafiati
Published: 4 June 2025
Last updated: 1 August 2025

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Journal articles on the topic "Fatty acyl desaturase 2"

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Chintalapati, Suresh, Jogadhenu Shyam Sunder Prakash, Pratima Gupta та ін. "A novel Δ9 acyl-lipid desaturase, DesC2, from cyanobacteria acts on fatty acids esterified to the sn−2 position of glycerolipids". Biochemical Journal 398, № 2 (2006): 207–14. http://dx.doi.org/10.1042/bj20060039.

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Acyl-lipid desaturases are enzymes that convert a C–C single bond into a C=C double bond in fatty acids that are esterified to membrane-bound glycerolipids. Four types of acyl-lipid desaturase, namely DesA, DesB, DesC, and DesD, acting at the Δ12, Δ15, Δ9, and Δ6 positions of fatty acids respectively, have been characterized in cyanobacteria. These enzymes are specific for fatty acids bound to the sn−1 position of glycerolipids. In the present study, we have cloned two putative genes for a Δ9 desaturase, designated desC1 and desC2, from Nostoc species. The desC1 gene is highly similar to the d
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Tavares, Sabina, Thomas Grotkjær, Thomas Obsen, Richard P. Haslam, Johnathan A. Napier та Nina Gunnarsson. "Metabolic Engineering ofSaccharomyces cerevisiaefor Production of Eicosapentaenoic Acid, Using a Novel Δ5-Desaturase fromParamecium tetraurelia". Applied and Environmental Microbiology 77, № 5 (2010): 1854–61. http://dx.doi.org/10.1128/aem.01935-10.

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ABSTRACTVery-long-chain polyunsaturated fatty acids, such as arachidonic acid (ARA), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA), have well-documented importance in human health and nutrition. Sustainable production in robust host organisms that do not synthesize them naturally requires the coordinated expression of several heterologous desaturases and elongases. In the present study we show production of EPA inSaccharomyces cerevisiaeusing glucose as the sole carbon source through expression of five heterologous fatty acid desaturases and an elongase. Novel Δ5-desaturases from
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Ding, Bao-Jian, Yi-Han Xia, Hong-Lei Wang, et al. "Biosynthesis of the Sex Pheromone Component (E,Z)-7,9-Dodecadienyl Acetate in the European Grapevine Moth, Lobesia botrana, Involving ∆11 Desaturation and an Elusive ∆7 Desaturase." Journal of Chemical Ecology 47, no. 3 (2021): 248–64. http://dx.doi.org/10.1007/s10886-021-01252-3.

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AbstractThe European grapevine moth, Lobesia botrana, uses (E,Z)-7,9-dodecadienyl acetate as its major sex pheromone component. Through in vivo labeling experiments we demonstrated that the doubly unsaturated pheromone component is produced by ∆11 desaturation of tetradecanoic acid, followed by chain shortening of (Z)-11-tetradecenoic acid to (Z)-9-dodecenoic acid, and subsequently introduction of the second double bond by an unknown ∆7 desaturase, before final reduction and acetylation. By sequencing and analyzing the transcriptome of female pheromone glands of L. botrana, we obtained 41 cand
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Zäuner, Simone, Wibke Jochum, Tara Bigorowski, and Christoph Benning. "A Cytochrome b 5 -Containing Plastid-Located Fatty Acid Desaturase from Chlamydomonas reinhardtii." Eukaryotic Cell 11, no. 7 (2012): 856–63. http://dx.doi.org/10.1128/ec.00079-12.

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ABSTRACT Monogalactosyldiacylglycerol (MGDG) in Chlamydomonas reinhardtii and other green algae contains hexadeca-4,7,10,13-tetraenoic acid (16:4) in the glycerol sn- 2 position. While many genes necessary for the introduction of acyl chain double bonds have been functionally characterized, the Δ4-desaturase remained unknown. Using a phylogenetic comparison, a candidate gene encoding the MGDG-specific Δ4-desaturase from Chlamydomonas (CrΔ4FAD) was identified. CrΔ4FAD shows all characteristic features of a membrane-bound desaturase, including three histidine boxes and a transit peptide for chlo
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NAKASHIMA, Shigeru, Yutong ZHAO та Yoshinori NOZAWA. "Molecular cloning of Δ9 fatty acid desaturase from the protozoan Tetrahymena thermophila and its mRNA expression during thermal membrane adaptation". Biochemical Journal 317, № 1 (1996): 29–34. http://dx.doi.org/10.1042/bj3170029.

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In response to a decrease in its growth temperature, the protozoan Tetrahymena is known to increase the level of unsaturated fatty acids in its membrane phospholipids so as to maintain the correct physical state (fluidity) of the membranes. In this organism, synthesis of unsaturated fatty acids is initiated by Δ9 acyl-CoA desaturase. Our previous studies have shown that, during cold adaptation, the activity of microsomal palmitoyl- and stearoyl-CoA desaturase increases, reaching a maximal level at 2 h after a temperature down-shift to 15 °C. Two hypotheses have been proposed to explain this in
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Griffiths, G., A. K. Stobart та S. Stymne. "Δ 6- and Δ12-desaturase activities and phosphatidic acid formation in microsomal preparations from the developing cotyledons of common borage (Borago officinalis)". Biochemical Journal 252, № 3 (1988): 641–47. http://dx.doi.org/10.1042/bj2520641.

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Microsomal membrane preparations from the maturing cotyledons of common borage (Borago officinalis) exhibit delta 12- and delta 6-desaturase activities, which resulted in the synthesis of linoleate and gamma-linolenate respectively. The desaturase enzymes utilized the complex lipid substrate phosphatidylcholine. The activity of these enzymes was sufficiently high to allow the monitoring of the mass changes in the endogenous oleate, linoleate and gamma-linolenate in the microsomal phosphatidylcholine in the presence of NADH (i.e. under desaturating conditions). The results illustrate that the d
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Chen, Xiao, Yi-Can Zhang, Si-Yu Zhang, Yi-Qian Zhou, and Na Cui. "Prediction of Omega-3 Fatty Acid Desaturase in Tomato." Biotechnology Journal International 29, no. 4 (2025): 14–24. https://doi.org/10.9734/bji/2025/v29i4782.

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Aims: The jasmonic acid (JA) pathway plays a core role in the plant defense response, and the omega-3 fatty acid desaturase (FAD7 protein) is closely related to the synthesis and signal transduction of JA by regulating fatty acid metabolism. However, the regulation differences of FAD7 in different plants and the correlation between the regulation of fatty acid metabolism and the dynamic balance of JA signaling remain unclear. Therefore, it has great significance to clarify the properties, structure and protein interaction network of the FAD7 protein for the study of the plant defense response
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Schmidt, H., and E. Heinz. "Direct desaturation of intact galactolipids by a desaturase solubilized from spinach (Spinacia oleracea) chloroplast envelopes." Biochemical Journal 289, no. 3 (1993): 777–82. http://dx.doi.org/10.1042/bj2890777.

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In plants, polyenoic fatty acids are synthesized by desaturase enzymes which use acyl groups of membrane lipids as substrates. To provide direct ‘in vitro’ evidence for this reaction, we solubilized envelope membranes from spinach (Spinacia oleracea) chloroplasts with Triton X-100 to release a membrane-bound n-6 desaturase. In the presence of oxygen and reduced ferredoxin, the solubilized enzyme desaturated a variety of substrates, such as free oleic acid, free erucic acid, 1-oleoyl-sn-glycerol 3-phosphate and the three galactolipids 1-oleoyl-2-(7′-cis-hexadecenoyl)-3-beta-D-galactopyranosyl-s
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Tian, Jieyun, Lu Tian, Ming Chen, Yabing Chen, and Anzhi Wei. "Low Temperature Affects Fatty Acids Profiling and Key Synthesis Genes Expression Patterns in Zanthoxylum bungeanum Maxim." International Journal of Molecular Sciences 23, no. 4 (2022): 2319. http://dx.doi.org/10.3390/ijms23042319.

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Zanthoxylum bungeanum is one of the most important medicinal and edible homologous plants because of its potential health benefits and unique flavors. The chemical components in compositions and contents vary with plant genotype variations and various environmental stress conditions. Fatty acids participate in various important metabolic pathways in organisms to resist biotic and abiotic stresses. To determine the variations in metabolic profiling and genotypes, the fatty acid profiling and key differential genes under low temperature stress in two Z. bungeanum varieties, cold-tolerant (FG) an
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Hu, Wei, Junyi Ma, Hongjie Zhang, Xin Miu, Xin Miao, and Yu Deng. "Integrated lipidomic and transcriptomic analysis reveals diacylglycerol accumulation in olive of Longnan (China)." PeerJ 11 (August 11, 2023): e15724. http://dx.doi.org/10.7717/peerj.15724.

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Background Olive (Olea europaea L.) oil accumulate more diacylglycerols (DAG) than mostly vegetable oils. Unsaturated fatty acids-enriched DAG consumption enhanced wellness in subjects. However, the mechanism of DAG accumulation is not yet fully understood. Methods In this study, gene network of DAG accumulation and fatty acid composition in the two olive mesocarps (“Chenggu 32” (CG) and “Koroneiki” (QJ)) were investigated by integrating lipidome and transcriptome techniques. Results A total of 1,408 lipid molecules were identified by lipidomic analysis in olive mesocarp, of which DAG (DAG36:3
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Dissertations / Theses on the topic "Fatty acyl desaturase 2"

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Zhang, Daiyuan. "Expression analysis of the fatty acid desaturase 2-4 and 2-3 genes from Gossypium hirsutum in transformed yeast cells and transgenic Arabidopsis plants." Thesis, University of North Texas, 2008. https://digital.library.unt.edu/ark:/67531/metadc9002/.

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Fatty acid desaturase 2 (FAD2) enzymes are phosphatidylcholine desaturases occurring as integral membrane proteins in the endoplasmic reticulum membrane and convert monounsaturated oleic acid into polyunsaturated linoleic acid. The major objective of this research was to study the expression and function of two cotton FAD2 genes (the FAD2-3 and FAD2-4 genes) and their possible role in plant sensitivity to environmental stress, since plants may increase the polyunsaturated phospholipids in membranes under environmental stress events, such as low temperature and osmotic stress. Two FAD2 cDNA
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Zhang, Daiyuan Pirtle Robert M. "Expression analysis of the fatty acid desaturase 2-4 and 2-3 genes from Gossypium hirsutum in transformed yeast cells and transgenic Arabidopsis plants." [Denton, Tex.] : University of North Texas, 2008. http://digital.library.unt.edu/permalink/meta-dc-9002.

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Huo, Tianyao. "Impact of fatty acyl composition and quantity of triglycerides on bioaccessibility of dietary carotenoids." The Ohio State University, 2007. http://rave.ohiolink.edu/etdc/view?acc_num=osu1195514528.

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Xu, Yi. "Development of a Novel Omega-6 Fatty Acid Based Treatment Strategy for Colon Cancer by Knocking Down Delta-5-Desaturase and Exploiting High COX-2 Levels in Cancer CellsTumors." Diss., North Dakota State University, 2017. http://hdl.handle.net/10365/25947.

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Colon cancer is the third most commonly diagnosed cancer in the world. Research showed that arachidonic acid, a downstream ?-6 fatty acid (?-6), plays a role in colon cancer development by producing deleterious metabolites from its COX-2 catalyzed peroxidation. On the other hand, dihomo-?-linolenic acid (DGLA), the immediate precursor of arachidonic acid, may represent an exceptional ?-6 associated with anti-cancer activities. However, the mechanism of DGLA?s anti-cancer effect still remains unclear, and the rapid conversion of DGLA to arachidonic acid in human body by delta-5 desaturase (D5D)
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Nampaisansuk, Mongkol. "Molecular cloning and analysis of the genes for cotton palmitoyl-acyl carrier protein thioesterase (PATE) and Δ-12 fatty acid desaturase (FAD2-3) and construction of sense and anti-sense PATE plasmid vectors for altering oilseed composition of transgenic cotton plants". Thesis, University of North Texas, 2002. https://digital.library.unt.edu/ark:/67531/metadc3123/.

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A cotton PATE cDNA clone has a 1.7-kb insert with an coding region for 410 amino acids, lacking codons for the three N-terminal amino acids. The predicted amino acid sequence of the PATE preprotein has a characteristic stromal-targeting domain and a 63% identity to the Arabidopsis FatB1 thioesterase sequence. A cotton genomic clone containing a 17.4-kb DNA segment was found to encompass a palmitoyl-ACP thioesterase (FatB1) gene. The gene spans 3.6 kb with six exons and five introns. The six exons are identical in nucleotide sequence to the open reading frame of the corresponding cDNA, and
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Revell, Kevin David. "Mode of action and structure-activity studies of N-alkylthio beta-lactams and N-alkylthio-2-oxazolidinones, and synthesis of second-generation disulfide Inhibitors of beta-Ketoacyl-Acyl Carrer Protein Synthase III (FabH) as potent antibacterial agents." [Tampa, Fla] : University of South Florida, 2006. http://purl.fcla.edu/usf/dc/et/SFE0001453.

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Jenkins, Benjamin John. "The role of alpha oxidation in lipid metabolism." Thesis, University of Cambridge, 2018. https://www.repository.cam.ac.uk/handle/1810/278025.

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Recent findings have shown an inverse association between the circulating levels of pentadecanoic acid (C15:0) and heptadecanoic acid (C17:0) with the risk of pathological development in type 2 diabetes, cardio vascular disease and neurological disorders. From previously published research, it has been said that both these odd chain fatty acids are biomarkers of their dietary intake and are significantly correlated to dietary ruminant fat intake. However, there are profound studies that show the contrary where they do not display this biomarker correlation. Additionally, several astute studies
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Gianngregorio, Alfredo. "The influence of fatty acids in vitro on mammalian cells from species differing in their fatty acyl desaturase capabilities. Volume. 2." Thesis, 1991. https://hdl.handle.net/10539/24356.

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A Thesis Submitted to the Faculty of Medicine, in Fulfilment of the Requirements for the Degree of • Doctor of Philosophy in the Department of Chemical Pathology, School of Pathology and the South African Institute for Medical Research, University of the Witwatersrand Medical School, Johannesburg, South Africa. December 1991.<br>Numerous studies have assessed the effects of single fatty acids on various aspects of lipid metabolism, particularly cancer. Established cell lines have largely been used for this purpose. The choice of control cells, however, has often been inappropriate. There i
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Lyle, Karen S. "Mechanism and function of soluble acyl-acyl carrier protein desaturase systems in fatty acid metabolism." 2003. http://www.library.wisc.edu/databases/connect/dissertations.html.

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Hsiao-Chun and 吳曉君. "Cloning of a Zebrafish Fatty Acid Desaturase 2." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/67215759595012103857.

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碩士<br>中山醫學大學<br>營養學研究所<br>95<br>Based on the data mining against NCBI（National Center for Biotechnology Information） data band. The analysis found a fatty acid desaturase-2 (FADS2) of human（NP_004256/NM_00426）and zebrafish（NP_571720.2/NM_131645）. The FADS2 cDNA of zebrafish were successfully amplified with a continuous open reading frame of 1335 bp encoding a protein of 444 amino acid. But the FADS2 cDNA of human were not successfully amplified with a continuous open reading frame of 1335 bp .The FADS2 of human and zebrafish were with a calculated molecular mass of 52.27 and 52.14 kDa . Zebra
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Book chapters on the topic "Fatty acyl desaturase 2"

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Frazier, Dianne. "Glutaric Acidemia Type I And 2-Ketoadipic Acidemia." In Pediatric Nutrition In Chronic Diseases And Developmental Disorders. Oxford University PressNew York, NY, 2005. http://dx.doi.org/10.1093/oso/9780195165647.003.0044.

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Abstract Glutaric acidemia type I (GAI), first described in 1975 by Goodman et al., is one of several rare autosomal recessive organic acidemias caused by a defect in the pathways of amino acid catabolism. It was designated “type I” to distinguish it from glutaric acidemia type II, or multiple acyl-coenzyme A (CoA) dehydrogenase deficiency, which is a defect of the mitochondrial respiratory chain affecting fatty acid oxidation as well as amino acid catabolism. A less well described disorder, 2-ketoadipic acidemia (KAA), results from an enzyme defect one step before the defect causing GAI. It t
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Masterson, Wayne J., and Anthony I. Magee. "Lipid modifications involved in protein targeting." In Protein Targeting. Oxford University PressOxford, 1992. http://dx.doi.org/10.1093/oso/9780199632060.003.0010.

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Abstract Several contributions in this volume describe the role of protein sequence motifs in acting as determinants of targeting specificity. In the last 12 years or so a group of post-translational modifications of proteins with various lipid molecules has been identified in eukaryotic cells (1). In many cases these modifications have been shown to be essential for the subcellular localization of the proteins carrying them. Many intracellular proteins are now known to carry fatty acid modifications which serve such a purpose. The 14 carbon saturated fatty acid myristate (C14:0) is amide-link
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Conference papers on the topic "Fatty acyl desaturase 2"

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"FATTY ACID ENZYME ACTIVITIES AND RISK OF DIABETES MELLITUS." In RAD Conference. RAD Centre, Niš, Serbia, 2024. https://doi.org/10.21175/radproc.2024.14.

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The prevalence of diabetes mellitus, especially type 2 diabetes (T2D), is high worldwide and there is an ongoing challenge to find potential biomarkers that could predict the onset of diabetes and provide insight into possible mechanisms for its development. Previously published data have shown that the composition and concentrations of free fatty acids (FFA) in plasma/serum are associated with the occurrence and risk of developing diabetes. Recent evidence suggests that the composition and content of free fatty acids are influenced by many factors, such as age, sex, ethnicity, dietary habits
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Ding, Tao, Li Pang, Jiang Liu, et al. "IDDF2022-ABS-0261 Fatty acid desaturase 1 mediating metabolic reprogramming of polyunsaturated fatty acids promotes HCC progression by impairing CD8+ T cell anti-tumor immunity." In Abstracts of the International Digestive Disease Forum (IDDF), Hong Kong, 2–4 September 2022. BMJ Publishing Group Ltd and British Society of Gastroenterology, 2022. http://dx.doi.org/10.1136/gutjnl-2022-iddf.29.

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Al-Qeraiwi, Maha, Manar Al-Rashid, Nasser Rizk, Abdelrahman El Gamal, and Amena Fadl. "Hepatic Gene Expression Profile of Lipid Metabolism of Obese Mice after treatment with Anti-obesity Drug." In Qatar University Annual Research Forum & Exhibition. Qatar University Press, 2020. http://dx.doi.org/10.29117/quarfe.2020.0214.

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Obesity is a global disorder with multifactorial causes. The liver plays a vital role in fat metabolism. Disorder of hepatic fat metabolism is associated with obesity and causes fatty liver. High fat diet intake (HFD) to mice causes the development of dietinduced obesity (DIO). The study aimed to detect the effects of anti-obesity drugs (sulforaphane; SFN and leptin) on hepatic gene expression of fat metabolism in mice that were fed HFD during an early time of DIO. Twenty wild types (WT) CD1 male mice aged ten weeks were fed a high fat diet. The mice were treated with vehicle; Veh (control gro
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Kerr, Matthew, Sara Rohling, Maria Sousa Fialho, Colleen Lopez, Damian Tyler, and Lisa C. Heather. "P32 Mitochondria must choose between resistance to fatty acyl coa regulation or rapid respiration in the type 2 diabetic heart." In British Society for Cardiovascular Research, Autumn Meeting 2017 ‘Cardiac Metabolic Disorders and Mitochondrial Dysfunction’, 11–12 September 2017, University of Oxford. BMJ Publishing Group Ltd and British Cardiovascular Society, 2018. http://dx.doi.org/10.1136/heartjnl-2018-bscr.37.

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Satsangi, Neera, Arpan Satsangi, Joo L. Ong, and Rajiv V. Satsangi. "Optimization of Phosphatidylserine-Modified Titanium Surfaces for Enhanced Osteoblast Response." In ASME 2006 International Manufacturing Science and Engineering Conference. ASMEDC, 2006. http://dx.doi.org/10.1115/msec2006-21053.

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This report is part of a continued effort to evaluate the in vitro osteoblast responses on different phospholipid coatings on Titanium (Ti) implant materials. It has been established that, among analogous phopholipids, the Ti surfaces coated with calcium phosphate (CaP) complex of phosphatidylserine induce the best calcium deposition and osteoblast growth and metabolism. This communication describes an effort to optimize the chemical structure of phosphatidylserine at its position−1 and −2, as Ti surface coating relative to enhancement in osteoblast differentiation and growth in culture. Four
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Llano, Sandra, Sonia Medina, Ana Muñoz Jimenez, Claudio Jiménez-Cartagena, and Julián Londoño-Londoño. "Untargeted metabolomics reveals specific withanolides and fatty acyl glycoside as tentative metabolites to differentiate organic and conventional Physalis peruviana fruits." In The 2nd International Electronic Conference on Metabolomics. MDPI, 2017. http://dx.doi.org/10.3390/iecm-2-04989.

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Purdon, A. D., and J. B. Smith. "RELEASE AND TRANSACYLATION OF ARACHIDONATE FROM A COMMON POOL OF 1-ACYL-2-ARACHIDONOYL GLYCEROPHOSPHOCHOLINE IN HUMAN PLATELETS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643391.

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We have previously shown that the main source of arachidonate in thrombin-stimulated human platelets is 1-acyl-2-arachidonoyl (AA) glycerophosphocholine (GPC) and release of 3H-AA from this phospholipid also was correlated with increased 3H-AA in ether phospholipid. This ATP independent transfer of 3H-AA from 1,2 diacyl GPC to ether phospholipid (transacylation) also occurs in resting cells. Human platelets in 1/10 volume of plasma (ACD anticoagulant, pH 6.5) were radiolabelled with 3H-AA for 60 min at 37°C and then exogenous 3H-AA was removed by gel filtration into Tyrode's buffer, pH 7.4, 0.
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Arsić, Aleksandra, Milica Kojadinović, Snjezana Petrović, Danijela Ristić Medić, Milena Žuža Praštalo, and Vesna Vučić. "FFECTS OF POMEGRANATE JUICE ON LIPID METABOLISM IN WOMEN WITH DYSLIPIDEMIA AND METABOLIC SYNDROME." In 2nd International Symposium on Biotechnology. Faculty of Agronomy in Čačak, University of Kragujevac, 2024. http://dx.doi.org/10.46793/sbt29.79aa.

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The objective of this research was to evaluate the effects of the consumption of Pomegranate juice (PJ) on blood pressure and lipid metabolism in subjects with dyslipidemia and metabolic syndrome. Twelve females with established dyslipidemia and 12 females with metabolic syndrome consumed 300 mL of PJ daily for 2 and 6 weeks respectively. PJ consumption resulted in a significant decrease in diastolic blood pressure, and LDL-cholesterol, an increase in the estimated activity of stearoyl-CoA desaturase in the short study, and a significant decrease in the percentage of arachidonic acid and an in
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Holcapek, Michal. "Comprehensive analysis of lipids: From plant oil analysis towards lipidomic cancer screening." In 2022 AOCS Annual Meeting & Expo. American Oil Chemists' Society (AOCS), 2022. http://dx.doi.org/10.21748/wqnw2781.

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The analysis of lipids as biomolecules involved in numerous metabolic pathways has attracted my attention since the beginning of my scientific carrier. My first article was published in 1999 on the monitoring of biodiesel production by LC/MS [1] and then continued with further work on the detailed characterization of triacylglycerols in plant oils and animal fats. We developed various LC/MS methods for the analysis of all existing types of triacylglycerol isomers, including regioisomers, double bond (DB) positional isomers [2], DB geometrical isomers [3] up to enantiomers [4]. Later, we moved
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Holcapek, Michal. "Potential of lipid class separation- mass spectrometry approaches for high-throughput lipidomic quantitation." In 2022 AOCS Annual Meeting & Expo. American Oil Chemists' Society (AOCS), 2022. http://dx.doi.org/10.21748/djkv2622.

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The coupling of liquid-phase separation techniques and mass spectrometry (MS) is the prevailing analytical strategy in lipidomic analysis [1]. Two basic chromatographic approaches are available, lipid class separation and lipid species separation. The lipid class separation is represented by hydrophilic liquid chromatography (HILIC) and ultrahigh-performance supercritical fluid chromatography (UHPSFC) and offers advantages for high-throughput quantitation due to the coelution of lipid class internal standards and analytes from the same class [2]. Multiple lipid classes from phospholipid, sphin
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Reports on the topic "Fatty acyl desaturase 2"

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Rafaeli, Ada, Wendell Roelofs, and Anat Zada Byers. Identification and gene regulation of the desaturase enzymes involved in sex-pheromone biosynthesis of pest moths infesting grain. United States Department of Agriculture, 2008. http://dx.doi.org/10.32747/2008.7613880.bard.

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The original objectives of the approved proposal included: 1. Establishment of the biosynthetic pathways for pheromone production using labeled precursors and GC-MS. 2. The elucidation of a circadian regulation of key enzymes in the biosynthetic pathway. 3. The identification, characterization and confirmation of functional expression of the delta-desaturases. 4. The identification of gene regulatory processes involved in the expression of the key enzymes in the biosynthetic pathway. Background to the topic: Moths constitute one of the major groups of pest insects in agriculture and their repr
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Sooksai, Sarintip, and Kobkul Laoteng. Genetic study of fatty acid and lipid biosynthesis in Hansenula polymorpha. Chulalongkorn University, 2011. https://doi.org/10.58837/chula.res.2011.121.

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Two groups of Hansenula polymorpha mutants were screened by their fatty acid requirement, one requires saturated fatty acids for growth (sfa- mutamts) and the other requires unsaturated fatty acids (mfa- and pfa- mutants). Two of the sfa- mutants, S7 and S16, showed significantly difference in the fatty acid composition. S7 clearly defected in the production of C18:2∆9,12 and C18:3∆9,12,15, while S16 significantly accumulated medium-chain saturated fatty acids, C12:0 and C14:0. By tetrad analysis, the results showed that S7 had double mutation which composed of fatty acid synthesis mutation (H
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Barg, Rivka, Kendal D. Hirschi, Avner Silber, Gozal Ben-Hayyim, Yechiam Salts, and Marla Binzel. Combining Elevated Levels of Membrane Fatty Acid Desaturation and Vacuolar H+ -pyrophosphatase Activity for Improved Drought Tolerance. United States Department of Agriculture, 2012. http://dx.doi.org/10.32747/2012.7613877.bard.

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Background to the topic: In previous works we have shown that Arabidopsis and tomato over-expressing H+-pyrophosphatase show increased tolerance to drought imposed by withholding irrigation of young plants in pots (Park et al. 2005). In addition, young tobacco plants over-expressing fatty acid desaturase 3 (OEX-FAD3) also showed increasing tolerance to drought stress (Zhang et al 2005), and similarly OEX-FAD3 young tomato plants (unpublished data from ARO), hence raising the possibility that pyramiding the two could further improve drought tolerance in tomato. Based on these findings the speci
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