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Dissertations / Theses on the topic 'Mycobacterial Proteins - Structural Analysis'

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Published: 4 June 2021

Last updated: 6 September 2023

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1

Castell, Alina. "Fighting Tuberculosis – : Structural Studies of Three Mycobacterial Proteins." Doctoral thesis, Uppsala universitet, Strukturell molekylärbiologi, 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-9348.

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This thesis presents the cloning, purification, crystallization, and structural studies of two unknown proteins from Mycobacterium tuberculosis, and of an aminotransferase from Mycobacterium smegmatis. Structural knowledge of these proteins is of highest interest for structure-based drug design, which is one of the approaches that can be used in order to fight tuberculosis (TB). The structure of the conserved hypothetical protein Rv0216 was refined to a resolution of 1.9 Å. The structure exhibits a so-called double hotdog-fold, similar to known hydratases. However, only parts of the hydratase

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2

Boonzaier, Jeremy. "Structural Analysis of Induced Mutagenesis Protein B from Mycobacterium tuberculosis Jeremy." University of the Western Cape, 2016. http://hdl.handle.net/11394/5720.

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Magister Scientiae - MSc (Biotechnology)<br>Knowing the three-dimensional structure of a protein may be useful in understanding its function. In this study, induced mutagenesis protein B (ImuB) from Mycobacterium tuberculosis was analyzed using molecular biology and molecular modelling techniques. The Rv3394c gene expressing ImuB was obtained from the group of Prof. Digby Warner at the Institute of Infectious Diseases and Molecular Medicine, University of Cape Town. Rv33974c was amplified from an expression plasmid using polymerase chain reaction (PCR) and inserted into multiple expression vec

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3

Kochva, Uzi. "Structural analysis of integral membrane proteins." E-thesis Full text (Hebrew University users only), 2007. http://shemer.mslib.huji.ac.il/dissertations/H/JSL/001449168.pdf.

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4

Siu, Wing-yan, and 蕭穎欣. "Multiple structural alignment for proteins." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2008. http://hub.hku.hk/bib/B4068748X.

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5

Anye, Valentine. "Structural analysis of induced mutagenesis A’ protein from mycobacterium tuberculosis and of a thermophillic GH9 cellulase." University of the Western Cape, 2014. http://hdl.handle.net/11394/4320.

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Masters of Science<br>The three-dimensional structures of proteins are important in understanding their function and interaction with ligands and other proteins. In this work, the structures of two proteins, ImuA’ from mycobacterium tuberculosis and GH9 C1 cellulase from a metagenomic library, were analysed using structural biological and modelling techniques. The gene encoding ImuA’ was amplified by two-step PCR, cloned, and expressed in E. coli. The recombinant ImuA’ produced was found to be largely insoluble. The insoluble protein was successfully solubilized in 8M urea but refolding the pr

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6

Berkowitz, Cheryl Anne. "Analysis of the structural proteins of rubella virus." Thesis, University of British Columbia, 1988. http://hdl.handle.net/2429/27798.

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Complications of rubella virus infection, including congenital rubella syndrome and the association of rubella virus with joint inflammation, emphasize the need for continued research on rubella virus. The finding that the association of rubella virus infection with joint manifestations is more pronounced with wild strains than with vaccine strains suggested the possibility of strain variation. Several different techniques have been employed in order to compare six rubella virus strains and identify variations in their structural proteins. Differences in biological activities were detected, i

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7

Hahn, Young Shin Lim Strauss James H. Strauss James H. "Functional analysis of viral nonstructural and structural proteins /." Diss., Pasadena, Calif. : California Institute of Technology, 1989. http://resolver.caltech.edu/CaltechETD:etd-06072007-075259.

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8

Dinis, Pedro Cleto Esteves Guerreiro. "Structural analysis of proteins from the radical SAM superfamily." Thesis, University of Southampton, 2016. https://eprints.soton.ac.uk/387225/.

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The Radical SAM superfamily is a large group of enzymes which use an iron‐sulfur cluster to catalyse the reductive cleavage of S‐adenosylmethionine (SAM), resulting in the formation of a highly reactive intermediate. This potent oxidant is used to functionalise relatively inert substrates to catalyse an extensive role of reactions: cofactor biosynthesis; anaerobic metabolism; methylation and post‐translational modifications. Most members of this family share some structural similarities, most notably a [4Fe‐4S] cluster, coordinated by a cysteine triad motif; some conserved motifs for the bindi

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9

Stahl, Morgan A. "The Perilipin Family of Proteins: Structural and Bioinformatic Analysis." Otterbein University Honors Theses / OhioLINK, 2005. http://rave.ohiolink.edu/etdc/view?acc_num=otbnhonors1620460421392971.

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10

Yarbrough, Daniel Kenneth. "Structural and mutational analysis of chromophore maturation in long wavelength fluorescent proteins /." view abstract or download file of text, 2004. http://wwwlib.umi.com/cr/uoregon/fullcit?p3120630.

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Thesis (Ph. D.)--University of Oregon, 2004.<br>Typescript. Includes vita and abstract. Includes bibliographical references (leaves 142-152). Also available for download via the World Wide Web; free to University of Oregon users.

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11

Madden, Peter William. "Structural and Kinetic Characterization of Myoglobins from Eurythermal and Stenothermal Fish Species." Fogler Library, University of Maine, 2003. http://www.library.umaine.edu/theses/pdf/MaddenPW2003.pdf.

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12

Rossmann, Maxim [Verfasser]. "Structural analysis of proteins of human sphingolipid metabolism / Maxim Rossmann." Berlin : Freie Universität Berlin, 2009. http://d-nb.info/1023263297/34.

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13

Martin, Claire Patricia. "Structural and functional analysis of NPA and FAR nematode proteins." Thesis, University of Glasgow, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.411838.

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14

Clark, Elizabeth Amber. "Structural and functional analysis of Staphylococcus aureus immune evasion proteins." Thesis, University of Bath, 2009. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.526317.

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Staphylococcus aureus is a common commensal organism and a clinically important pathogen. S. aureus is able to establish infection in a range of tissues owing to its production of a wide variety of proteins which aid its invasion, attachment and immune evasion. Characterisation of S. aureus proteins is required to deepen understanding of infection and immunity and for continued development of antibacterial therapy. Extracellular fibrinogen binding protein, Efb, binds both fibrinogen and complement components. The C-terminal complement modulating domain of Efb, Efb-C, is well characterised. NMR

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15

Sahul, Hamid Nuraini. "Structural analysis of interactions between kinesin motor proteins and microtubules." Thesis, University of Cambridge, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.611895.

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16

Hitchings, Matthew D. "Biochemical and structural analysis of the Streptomyces coelicolor Dps proteins." Thesis, Swansea University, 2013. https://cronfa.swan.ac.uk/Record/cronfa42992.

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The three DNA protection proteins from starved cells (Dps) of Streptomyces coelicolor are members of the min-ferritin super family. Considered to be of major importance to stress response systems in microorganisms. Dps proteins can aid microbial survival in extreme conditions. The S. coelicolor Dps proteins are not only induced in response to stress in a stimulus-dependent manner, but dual regulation allows these proteins to play a role in bacterial cell division; influencing condensation of nucleoids during spore formation. This study investigates the structural and functional properties of t

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17

Kojic, Igor. "An NMR analysis of the structure and ligand binding characteristics of proteins from Mycobacterium tuberculosis implicated in dormancy." Thesis, University College London (University of London), 2008. http://discovery.ucl.ac.uk/1444286/.

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Two proteins putatively involved in the persistence of Mycobacterium tuberculosis infection have been investigated by heteronuclear nuclear magnetic resonance (NMR) spectroscopy and other methods. The major aspect of work describes the characterization by NMR of the Mtb thiol peroxidase TPx. TPx (165 residues) is a 36 kD symmetrical homodimer that catalyzes the reduction of a range of reactive oxygen species in vitro. Multidimensional heteronuclear NMR spectroscopy was applied to recombinant wild-type (Wt) and Cys60→Ser (C60S) TPx, both of which are clearly folded but display slightly differen

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18

Leon, Ronald P. "Structural and functional analysis of MCM helicases in eukaryotic DNA replication /." Connect to full text via ProQuest. Limited to UCD Anschutz Medical Campus, 2007.

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Thesis (Ph.D. in Biophysics & Genetics, Program in Molecular Biology) -- University of Colorado Denver, 2007.<br>Typescript. Includes bibliographical references (leaves 90-98). Free to UCD affiliates. Online version available via ProQuest Digital Dissertations;

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19

Taylor, Charles Dariush. "Structural characterisation and analysis of human cripto-1." Thesis, University of Oxford, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.670042.

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20

Yau, Peter Mo-Ping. "Structural analysis of the nucleosome and the effects of histone acetylation." Thesis, Liverpool John Moores University, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.261657.

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21

Enders, Oliver. "Structural analysis of biological membranes and proteins by atomic force microscopy." [S.l. : s.n.], 2004. http://deposit.ddb.de/cgi-bin/dokserv?idn=972570497.

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22

Najmudin, Shabir. "A structural analysis of eye lens proteins : refinement of yB crystallin." Thesis, Birkbeck (University of London), 1993. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.740998.

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23

Greving, Imke. "Structural analysis of silk proteins using x–ray and neutron scattering." Thesis, University of Oxford, 2012. http://ora.ox.ac.uk/objects/uuid:914b23c5-4f95-4f8f-90c6-d04c3a3e2a6c.

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The silk fibres spun by insects and spiders have intrigued scientists for many years. Their mechanical performance is remarkable when one considers that the fibres are spun under ambient conditions from aqueous protein solutions without requiring many of the harsh processing conditions used in the production of man-made fibres. Yet, despite this interest, very little is known about the initial structure of the precursor proteins prior to spinning. One reason for this lies in the difficulty of handling the native proteins without accidental aggregation. Therefore in this thesis a novel sample p

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24

Cotton, Victoria Emma. "A structural and functional analysis of mismatch repair proteins in meiosis." Thesis, University of Leicester, 2007. http://hdl.handle.net/2381/30372.

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A number of the mismatch repair proteins facilitate recombination during meiosis. Among these are the MutS homologues, Msh4p and Msh5p, the MutL homologues M1h1p and M1h3p, and the exonuclease Exo1p. Msh4p and Msh5p are meiosis specific proteins, which function to promote crossing over, although the mechanism is unknown. M1h1p and M1h3p are involved in the same pathway, but are thought to act later during meiosis as the meiotic phenotypes of m1h1Delta and m1h3Delta are less severe than those of msh4Delta or msh5Delta. Structure and function studies of M1h1p have begun to elucidate its roles in

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25

Sargaeva, Nadezda P. "Deamidation and related problems in structural analysis of peptides and proteins." Thesis, Boston University, 2012. https://hdl.handle.net/2144/12612.

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Thesis (Ph.D.)--Boston University PLEASE NOTE: Boston University Libraries did not receive an Authorization To Manage form for this thesis or dissertation. It is therefore not openly accessible, though it may be available by request. If you are the author or principal advisor of this work and would like to request open access for it, please contact us at open-help@bu.edu. Thank you.<br>Electron capture dissociation (ECD) and electron transfer dissociation (ETD) can generate unique fragments and preserve post-translational modifications (PTMs), enabling their detection in biological samples. T

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26

Sjekloca, Ljiljana. "Structural analysis of human striated muscle proteins: FATZ and γ-filamin". Doctoral thesis, SISSA, 2005. http://hdl.handle.net/20.500.11767/4670.

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The aim of the study we present in this PhD thesis is to gain a deeper insight into structure of Z-disc proteins FATZ 1 and y-filamin. Z-discs are multi-protein complexes which are the primary conduits of the force generated by striated muscle contraction. The protein composition of Z-disc is not well defined and new proteins are continuously being reported. FATZ 1 is expressed early during myofibrilogenesis and it is presumed to have an important role in Z-disc assembly and functioning. At the moment, it is the only Z-disc protein for which a direct connection with the signal transducer pro

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27

Vit, Katharina Allegra [Verfasser], and Wulf [Akademischer Betreuer] Blankenfeldt. "Structural and Functional Studies on beta-alpha-beta-beta-beta-Module Resistance Proteins from Pseudomonas aeruginosa PAO1 and Structural Insights into Mycobacterial Ergothioneine Biosynthesis / Katharina Allegra Vit ; Betreuer: Wulf Blankenfeldt." Braunschweig : Technische Universität Braunschweig, 2015. http://d-nb.info/1175818577/34.

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28

Masciangioli, Tina Marie. "Structural and dynamic studies of bacteriorhodopsin and its variants." Diss., Georgia Institute of Technology, 1999. http://hdl.handle.net/1853/30551.

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29

Rennell, Dale. "A Genetic and Structural Analysis of P22 Lysozyme: A Thesis." eScholarship@UMMS, 1988. https://escholarship.umassmed.edu/gsbs_diss/238.

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P22 lysozyme, encoded by gene 19, is an essential phage protein responsible for hydrolyzing the bacterial cell wall during lytic infection. P22 lysozyme is related to T4 lysozymein its mode of action, substrate specificities, and in its structure. Gene 19 was located on the phage genome, subcloned, and then sequenced. lysozyme was produced in large quantities and purified for biochemical characterization and for crystallograpic studies. Gene 19consists of 146 codons, and encodes a protein with a molecular weight of 16,117. Amber mutations were created in gene 19 by in vitro primer-directed mut

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30

Smith, Corey Lewis. "Functional and Structural Analysis of the Yeast SWI/SNF Complex: a Dissertation." eScholarship@UMMS, 2004. https://escholarship.umassmed.edu/gsbs_diss/13.

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Modulating chromatin structure is an important step in maintaining control over the eukaryotic genome. SWI/SNF, one of the complexes belonging to the growing family of ATP-dependent chromatin remodeling enzymes, is involved in controlling the expression of a number of inducible genes whose proper regulation is vital for metabolism and progression through mitosis. The mechanism by which SWI/SNF modulates chromatin structure at the nucleosome level is an important aspect of this regulation. The work in this dissertation focuses on how the Saccharomyces cerevisiae SWI/SNF complex uses the energy

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31

Cavignac, Yolaine. "Proteomic analysis of cytomegalovirus structural proteins and of HCMV-infected endothelial cells." Göttingen Sierke, 2008. http://d-nb.info/987521543/04.

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32

Gendoo, Deena. "Bioinformatic sequence and structural analysis for Amyloidogenicity in Prions and other proteins." Thesis, McGill University, 2012. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=110518.

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Detection of amyloidogenic peptides or domains in proteins is of paramount importance towards understanding their role in amyloidosis in conformational diseases. This thesis explores different methods towards detection and prediction of amyloidogenic peptides using a variety of bioinformatic analytical methods. Bioinformatic analysis of secondary structural changes is employed to determine whether classes of structurally ambivalent peptides, mainly discordant and chameleon sequences, are efficient predictors of amyloidogenic segments. This analysis elucidates statistical relationships between

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33

Wang, Yang [Verfasser], and Kirsten [Akademischer Betreuer] Jung. "Evolutionary and structural analysis of KdpD proteins / Yang Wang ; Betreuer: Kirsten Jung." München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2017. http://d-nb.info/1188200798/34.

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34

Kimura, Aiko. "Analysis of Structural Factors Contributing to Physicochemical Properties of Seed Storage Proteins." Kyoto University, 2010. http://hdl.handle.net/2433/120460.

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Kyoto University (京都大学)<br>0048<br>新制・課程博士<br>博士(農学)<br>甲第15416号<br>農博第1801号<br>新制||農||978(附属図書館)<br>学位論文||H22||N4515(農学部図書室)<br>27894<br>京都大学大学院農学研究科農学専攻<br>(主査)准教授丸山伸之, 教授松村康生, 教授谷坂隆俊<br>学位規則第4条第1項該当

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35

Pandya, Ashka Y. "Structural and functional analysis of KLF4." Thesis, Birmingham, Ala. : University of Alabama at Birmingham, 2007. https://www.mhsl.uab.edu/dt/2009r/pandya.pdf.

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36

Yates, Luke Alexander. "Structural studies in cell adhesion and division." Thesis, University of Oxford, 2012. http://ora.ox.ac.uk/objects/uuid:d66f5602-7e49-4042-8ebf-9457e61d56c3.

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Cell adhesion is a critical process that allows the organisation and functioning of tissues in three-dimensions. However, the replenishing of cells, via cell division, within tissues is equally important for functioning complex life. Both cell adhesion and division are tightly controlled processes and rely on a complex network of signals that, as yet, are not wholly understood. This Thesis presents a structural analysis of several proteins involved in these processes. In the case of cell adhesion, we have made use of high-throughput (HTP) cloning and expression screening technologies in the Ox

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37

Mark, Charlotta. "Three Subfamilies of KRAB Zinc Finger Proteins : A Structural, Functional and Evolutionary Analysis." Doctoral thesis, Uppsala University, Department of Cell and Molecular Biology, 2003. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-3512.

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<p>Krüppel-related zinc finger proteins constitute the largest single class of transcription factors within the human genome. Members of this protein family have the ability to either activate or repress transcription depending on the presence of specific activator or repressor domains within the protein. Approximately one third of the Krüppel-related zinc finger proteins contain an evolutionarily well-conserved repressor domain termed the KRAB domain. This domain acts as a potent repressor of transcription by interacting with the co-repressor protein, TIF1β. TIF1β then, in turn, recruits HP1

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38

Schiller, Christian Bernd. "Structural and functional analysis of the eukaryotic DNA repair proteins Mre11 and Nbs1." Diss., lmu, 2011. http://nbn-resolving.de/urn:nbn:de:bvb:19-134006.

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39

Bush, Martin. "Pilot studies on the production of proteins and protein complexes for structural analysis." Thesis, University of Sheffield, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.521908.

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40

Self, Annette Jane. "Structural and functional analysis of Ras and Ruo-related small GTP-binding proteins." Thesis, Institute of Cancer Research (University Of London), 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.266353.

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41

Diekmann, Dagmar. "Structural and functional analysis of the small GTP-binding proteins rho and rac." Thesis, Institute of Cancer Research (University Of London), 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.283195.

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42

Carrara, Guia. "Structural and functional analysis of human and viral Golgi anti-apoptotic proteins (GAAP)." Thesis, University of Cambridge, 2014. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.708153.

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43

Masood, Rehana [UNESP]. "Production, characterization and structural analysis of proteins from Corynebacterium pseudotuberculosis and snake venoms." Universidade Estadual Paulista (UNESP), 2015. http://hdl.handle.net/11449/138506.

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Made available in DSpace on 2016-05-17T16:51:40Z (GMT). No. of bitstreams: 0 Previous issue date: 2015-10-19. Added 1 bitstream(s) on 2016-05-17T16:55:27Z : No. of bitstreams: 1 000864535_20161230.pdf: 356823 bytes, checksum: b1429fb38bc530053d5caf023608ebf0 (MD5) Bitstreams deleted on 2017-01-02T15:03:58Z: 000864535_20161230.pdf,. Added 1 bitstream(s) on 2017-01-02T15:05:11Z : No. of bitstreams: 1 000864535.pdf: 3933353 bytes, checksum: 8bf44adf010cf6c18e755c884adcb9da (MD5)<br>Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)<br>Corynebacterium pseudotuberculosis (C. pse

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Masood, Rehana. "Production, characterization and structural analysis of proteins from Corynebacterium pseudotuberculosis and snake venoms /." São José do Rio Preto, 2015. http://hdl.handle.net/11449/138506.

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Orientador: Raghuvir Krishnaswamy Arni<br>Banca: Patrick Jack Spencer<br>Banca: Marcos Roberto de Mattos Fontes<br>Banca: João Ruggiero Neto<br>Banca: Gustavo Orlando Bonilla Rodriguez<br>Resumo: Corynebacterium pseudotuberculosis (C. pseudotuberculosis) é o agente etiológico da linfadenite caseosa (CLA), uma doença que afeta diferentes grupos de animais em todo o mundo, especificamente áreas de produção de ovinos e caprinos, que causam perdas econômicas significativas. Estas bactérias podem infectar humanos, e 25 casos de infecções em humanos foram relatados na literatura. Atualmente, não há

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Xu, Qifang. "Statistical Analysis of Biological Interactions from Homologous Proteins." Diss., Temple University Libraries, 2008. http://cdm16002.contentdm.oclc.org/cdm/ref/collection/p245801coll10/id/25686.

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Computer and Information Science<br>Ph.D.<br>Information fusion aims to develop intelligent approaches of integrating information from complementary sources, such that a more comprehensive basis is obtained for data analysis and knowledge discovery. Our Protein Biological Unit (ProtBuD) database is the first database that integrated the biological unit information from the Protein Data Bank (PDB), Protein Quaternary Server (PQS) and Protein Interfaces, Surfaces and Assemblies (PISA) server, and compared the three biological units side-by-side. The statistical analyses show that the inconsisten

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46

Saikrishnan, K. "Structural Studies On Mycobacterial Proteins." Thesis, 2005. https://etd.iisc.ac.in/handle/2005/1496.

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Saikrishnan, K. "Structural Studies On Mycobacterial Proteins." Thesis, 2005. http://etd.iisc.ernet.in/handle/2005/1496.

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48

Saraswathi, Ramachandran. "Starvation Response In Mycobacterium Smegmatis : A Tale Of Two Proteins." Thesis, 2009. https://etd.iisc.ac.in/handle/2005/918.

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The Dps (DNA-Binding Protein from Starved Cells) proteins are a class of stress-specific proteins with a major role in protecting DNA during the stationary phase of bacterial growth, through direct physical binding as well as ferroxidation. These proteins are characteristically dodecameric in nature. Mycobacterium smegmatis, which is the model organism used in this study has two Dps homologues- MsDps1 and MsDps2. MsDps1, that has previously been studied, is exceptional in having trimeric as well as dodecameric states in vitro. This work focuses on the functional domains of MsDps1, with resp

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Saraswathi, Ramachandran. "Starvation Response In Mycobacterium Smegmatis : A Tale Of Two Proteins." Thesis, 2009. http://hdl.handle.net/2005/918.

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The Dps (DNA-Binding Protein from Starved Cells) proteins are a class of stress-specific proteins with a major role in protecting DNA during the stationary phase of bacterial growth, through direct physical binding as well as ferroxidation. These proteins are characteristically dodecameric in nature. Mycobacterium smegmatis, which is the model organism used in this study has two Dps homologues- MsDps1 and MsDps2. MsDps1, that has previously been studied, is exceptional in having trimeric as well as dodecameric states in vitro. This work focuses on the functional domains of MsDps1, with resp

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50

Bhagavat, Raghu B. R. "Structural Bioinformatics of Ligand Recognition in Proteins : A large-scale Analysis and Applications in Drug Discovery." Thesis, 2017. http://etd.iisc.ac.in/handle/2005/4249.

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Biological processes are governed by highly specific macromolecular interactions. Understanding the precise mechanism of ligand recognition in proteins is essential for deriving features responsible for such recognition capabilities. Although protein sequences give first-hand information about their function, their three-dimensional structures, which are the evolutionary units, convey the function better. Three-dimensional structures of many proteins determined through X-ray crystallography and/or NMR are available in the Protein Data Bank, a public repository. This resource has stimulated the

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