Relevant bibliographies by topics / Usp gene

Contents

  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers
  6. Reports

Academic literature on the topic 'Usp gene'

Author: Grafiati
Published: 3 June 2025
Last updated: 31 July 2025

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Journal articles on the topic "Usp gene"

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Alieh, Lotfi Nejad Mansour Amin Shekoofeh Sadat Etemadzadeh Mohammad Hossein Rezaeian. "DISTRIBUTION OF THE USP GENE IN UROPATHOGENIC ESCHERICHIA COLI FROM PATIENTS WITH URINARY TRACT INFECTION AND INVESTIGATION OF ANTIBIOTIC RESISTANCE." Indo American Journal of Pharmaceutical Sciences 04, no. 10 (2017): 3451–57. https://doi.org/10.5281/zenodo.1001732.

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Urinary tract infection (UTI) is one of the most frequent infectious diseases around the world that caused by uropathogenic Escherichia coli (UPEC). Uropathogenic-specific protein (usp) is homologous to the Vibrio cholerae zonula occludens toxin gene wich is found in all common serogroups of UPEC. this study was performed to detect the distribution of usp and antibiotic resistance properties in E. coli strains isolated from patients with UTIs. For this purpose, samples were collected from patients and the strains which were biochemically confirmed as E. coli- positive used for detection of usp
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Lai, Yun Mei, Myo Thura Zaw, Shamsul Bahari Shamsudin, and Zaw Lin. "Evaluation of PAIusp subtyping to characterize uropathogenic E. coli isolates." Journal of Infection in Developing Countries 10, no. 10 (2016): 1053–58. http://dx.doi.org/10.3855/jidc.6944.

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Introduction: Uropathogenic virulence factors have been identified by comparing the prevalence of these among urinary tract isolates and environmental strains. The uropathogenic-specific protein (USP) gene is present on the pathogenicity island (PAI) of uropathogenic Escherichia coli (UPEC) and, depending on its two diverse gene types and the sequential patterns of three open reading frame units (orfUs) following it, there is a method to characterize UPEC epidemiologically called PAIusp subtyping. Methodology: A total of 162 UPEC isolates from Sabah, Malaysia, were tested for the presence of t
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Kato, Yasuhiko, Kaoru Kobayashi, Shigeto Oda, Norihisa Tatarazako, Hajime Watanabe, and Taisen Iguchi. "Cloning and characterization of the ecdysone receptor and ultraspiracle protein from the water flea Daphnia magna." Journal of Endocrinology 193, no. 1 (2007): 183–94. http://dx.doi.org/10.1677/joe-06-0228.

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cDNAs encoding the ecdysone receptor (EcR) and ultra spiracle (USP) protein were cloned from the water flea Daphnia magna (Crustacea: Cladocera). The deduced EcR and USP amino acid sequences showed a high degree of homology to those of other crustaceans as well as insects. We isolated three isoforms of EcR that differ in the A/B domain. Quantitative PCR analysis indicated differing temporal expression patterns of the EcR isoforms during the molting period and demonstrated that the expression of one subtype correlated well with the timing of molt. Using cDNAs encoding EcR and USP, we constructe
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Isokpehi, Raphael D., Shaneka S. Simmons, Angela U. Makolo, et al. "Insights into Functions of Universal Stress Proteins Encoded by Genomes of Gastric Cancer Pathogen Helicobacter pylori and Related Bacteria." Pathogens 14, no. 3 (2025): 275. https://doi.org/10.3390/pathogens14030275.

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The genes that encode the universal stress protein (USP) family domain (pfam00582) aid the survival of bacteria in specific host or habitat-induced stress conditions. Genome sequencing revealed that the genome of Helicobacter pylori, a gastric cancer pathogen, typically contains one USP gene, while related helicobacters have one or two distinct USP genes. However, insights into the functions of Helicobacteraceae (Helicobacter and Wolinella) USP genes are still limited to inferences from large-scale genome sequencing. Thus, we have combined bioinformatics and visual analytics approaches to cond
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BERGMAN, Thomas, Vincent C. HENRICH, Uwe SCHLATTNER, and Markus LEZZI. "Ligand control of interaction in vivo between ecdysteroid receptor and ultraspiracle ligand-binding domain." Biochemical Journal 378, no. 3 (2004): 779–84. http://dx.doi.org/10.1042/bj20031302.

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Ecdysteroids (Ecs) enhance the formation of Ec receptor–ultraspiracle protein (EcR–USP) heterodimers which regulate gene transcription. To study EcR–USP heterodimerization, fusion proteins were constructed from the LBDs (ligand-binding domains) of Drosophila EcR or USP and the activation or DNA-binding region of GAL4 respectively. Reporter gene (lacZ) activation was fully dependent on the co-expression of the two fusion proteins and thus constitutes a reliable measure for the interaction in vivo between the two LBDs in the yeast cell. To identify structures involved in heterodimerization, a to
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Lan, Que, Kiyoshi Hiruma, Xiao Hu, Marek Jindra, and Lynn M. Riddiford. "Activation of a Delayed-Early Gene Encoding MHR3 by the Ecdysone Receptor Heterodimer EcR-B1–USP-1 but Not by EcR-B1–USP-2." Molecular and Cellular Biology 19, no. 7 (1999): 4897–906. http://dx.doi.org/10.1128/mcb.19.7.4897.

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ABSTRACT MHR3, a homolog of the retinoid orphan receptor (ROR), is a transcription factor in the nuclear hormone receptor family that is induced by 20-hydroxyecdysone (20E) in the epidermis of the tobacco hornworm, Manduca sexta. Its 2.7-kb 5′ flanking region was found to contain four putative ecdysone receptor response elements (EcREs) and a monomeric (GGGTCA) nuclear receptor binding site. Activation of this promoter fused to a chloramphenicol acetyltransferase (CAT) reporter by 2 μg of 20E per ml inManduca GV1 cells was similar to that of endogenous MHR3, with detectable CAT by 3 h. When th
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Galant, Christine, Joel Marchandise, Maria S. Stoenoiu та ін. "Overexpression of ubiquitin-specific peptidase 15 in systemic sclerosis fibroblasts increases response to transforming growth factor β". Rheumatology 58, № 4 (2019): 708–18. http://dx.doi.org/10.1093/rheumatology/key401.

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Abstract Objective Ubiquitination of proteins leads to their degradation by the proteasome, and is regulated by ubiquitin ligases and substrate-specific ubiquitin-specific peptidases (USPs). The ubiquitination process also plays important roles in the regulation of cell metabolism and cell cycle. Here, we found that the expression of several USPs is increased in SSc tenosynovial and skin biopsies, and we demonstrated that USP inhibition decreases TGF-β signalling in primary fibroblast cell lines. Methods High-density transcriptomic studies were performed using total RNA obtained from SSc tenos
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Rihtar, Erik, Darja Žgur Bertok, and Zdravko Podlesek. "The Uropathogenic Specific Protein Gene usp from Escherichia coli and Salmonella bongori is a Novel Member of the TyrR and H-NS Regulons." Microorganisms 8, no. 3 (2020): 330. http://dx.doi.org/10.3390/microorganisms8030330.

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The Escherichia coli PAIusp is a small pathogenicity island encoding usp, for the uropathogenic specific protein (Usp), a genotoxin and three associated downstream imu1-3 genes that protect the producer against its own toxin. Bioinformatic analysis revealed the presence of the PAIusp also in publically available Salmonella bongori and Salmonella enterica subps. salamae genome sequences. PAIusp is in all examined sequences integrated within the aroP-pdhR chromosomal intergenic region. The focus of this work was identification of the usp promoter and regulatory elements controlling its activity.
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Hall, B. L., and C. S. Thummel. "The RXR homolog ultraspiracle is an essential component of the Drosophila ecdysone receptor." Development 125, no. 23 (1998): 4709–17. http://dx.doi.org/10.1242/dev.125.23.4709.

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Pulses of the steroid hormone ecdysone function as key temporal signals during insect development, coordinating the major postembryonic developmental transitions, including molting and metamorphosis. In vitro studies have demonstrated that the EcR ecdysone receptor requires an RXR heterodimer partner for its activity, encoded by the ultraspiracle (usp) locus. We show here that usp exerts no apparent function in mid-third instar larvae, when a regulatory hierarchy prepares the animal for the onset of metamorphosis. Rather, usp is required in late third instar larvae for appropriate developmenta
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Brodu, Véronique, Bruno Mugat, Jean-Yves Roignant, Jean-Antoine Lepesant, and Christophe Antoniewski. "Dual Requirement for the EcR/USP Nuclear Receptor and the dGATAb Factor in an Ecdysone Response inDrosophila melanogaster." Molecular and Cellular Biology 19, no. 8 (1999): 5732–42. http://dx.doi.org/10.1128/mcb.19.8.5732.

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ABSTRACT The EcR/USP nuclear receptor controls Drosophilametamorphosis by activating complex cascades of gene transcription in response to pulses of the steroid hormone ecdysone at the end of larval development. Ecdysone release provides a ubiquitous signal for the activation of the receptor, but a number of its target genes are induced in a tissue- and stage-specific manner. Little is known about the molecular mechanisms involved in this developmental modulation of the EcR/USP-mediated pathway. Fbp1 is a good model of primary ecdysone response gene expressed in the fat body for addressing thi
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Dissertations / Theses on the topic "Usp gene"

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ZELUS, DOMINIQUE. "Evolution des systemes de communication intercellulaire : l'interleukine-2 et le recepteur nucleaire usp (doctorat : parasitologie)." Lille 2, 1999. http://www.theses.fr/1999LIL2T008.

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Burtscher, Ingo Markus. "Characterisation of USP-25, a chromosome 21 gene potentially contributing to specific features of Down syndrome, and the effects of its overexpression on cellular structure, function and behaviour." Thesis, Queen Mary, University of London, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.415908.

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Santos, Valdir Azevedo dos. "Caracterização molecular de isolados de Giardia spp. provinientes de amostras fecais de origem humana do Hospital Universitário - USP - São Paulo, pela análise de fragmentos do gene codificador da beta-giardina (bg)." Universidade de São Paulo, 2011. http://www.teses.usp.br/teses/disponiveis/42/42135/tde-21032012-154629/.

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A giardíase é uma doença entérica de alta prevalência particularmente nos países em desenvolvimento. Diferentes espécies foram descritas em função de seus hospedeiros. A G. duodenalis é a espécie que parasito não só o homem, mas também animais domésticos e silvestres. Recentemente, com a aplicação de técnicas moleculares, foi possível identificar sete diferentes agrupamentos sendo que cada um deles tem predileção por determinadas espécies de hospedeiro. Essa discriminação não é possível por meio da microscopia. A identificação do(s) agrupamento(s) presente(s) na população de determinada região
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Blacha, Anna Maria. "Investigating the role of regulatory genes in heterosis for superior growth and biomass production in Arabidopsis thaliana." Phd thesis, Universität Potsdam, 2009. http://opus.kobv.de/ubp/volltexte/2010/4614/.

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‘Heterosis’ is a term used in genetics and breeding referring to hybrid vigour or the superiority of hybrids over their parents in terms of traits such as size, growth rate, biomass, fertility, yield, nutrient content, disease resistance or tolerance to abiotic and abiotic stress. Parental plants which are two different inbred (pure) lines that have desired traits are crossed to obtain hybrids. Maximum heterosis is observed in the first generation (F1) of crosses. Heterosis has been utilised in plant and animal breeding programs for at least 90 years: by the end of the 21st century, 65% of wo
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Arana-Ceballos, Fernando Alberto. "Biochemical and physiological studies of Arabidopsis thaliana Diacylglycerol Kinase 7 (AtDGK7)." Phd thesis, kostenfrei, 2006. http://opus.kobv.de/ubp/volltexte/2007/1372/.

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Hsia, Kan-Tai. "Use of gene targeting to study the mouse ERCC1 gene." Thesis, University of Edinburgh, 2000. http://hdl.handle.net/1842/14118.

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The main aims of this work to set up two new mouse models, by conventional and conditional gene targeting, to investigate the functions of ERCC1, which has not, thus far, been associated with any known human disorders. To facilitate gene targeting studies, a series of overlapping ERCC1 genomic clones were retrieved from a bacteriophage library. Analysis of the mouse ERCC1 5'-flanking region revealed a novel transcription initiation site. Furthermore, several potential liver- and brain-specific transcription factor binding sites were present. A novel ERCC1-deficient mouse line, designated ET#20
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Frömmel, Ulrike. "Vergleichende geno- und phänotypische Charakterisierung von Escherichia coli aus Menschen, Hausschweinen und Wildtieren." Phd thesis, Universität Potsdam, 2013. http://opus.kobv.de/ubp/volltexte/2014/6914/.

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Escherichia (E.) coli ist als kommensales Bakterium ein wichtiger Bestandteil des Mikrobioms von Säugern, jedoch zudem der häufigste Infektionserreger des Menschen. Entsprechend des Infektionsortes werden intestinal (InPEC) und extraintestinal pathogene E. coli (ExPEC) unterschieden. Die Pathogenese von E. coli-Infektionen ist durch Virulenzfaktoren determiniert, welche von jeweils spezifischen virulenzassoziierten Genen (inVAGs und exVAGs) kodiert werden. Häufig werden exVAGs auch in E. coli-Isolaten aus dem Darm gesunder Wirte nachgewiesen. Dies führte zu der Vermutung, dass exVAGs die intes
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Schütte, Moritz. "Evolutionary fingerprints in genome-scale networks." Phd thesis, Universität Potsdam, 2011. http://opus.kobv.de/ubp/volltexte/2012/5748/.

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Mathematical modeling of biological phenomena has experienced increasing interest since new high-throughput technologies give access to growing amounts of molecular data. These modeling approaches are especially able to test hypotheses which are not yet experimentally accessible or guide an experimental setup. One particular attempt investigates the evolutionary dynamics responsible for today's composition of organisms. Computer simulations either propose an evolutionary mechanism and thus reproduce a recent finding or rebuild an evolutionary process in order to learn about its mechanism. The
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George, Florence. "Johnson's system of distributions and microarray data analysis." [Tampa, Fla.] : University of South Florida, 2007. http://purl.fcla.edu/usf/dc/et/SFE0002040.

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Hedlund, Isa. "The ANKK1 Gene and its Possible Influence on Alcohol Use: : The Role of Victimization and Parent-Child Relationship." Thesis, Örebro universitet, Institutionen för juridik, psykologi och socialt arbete, 2020. http://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-86951.

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Risky alcohol use increases the risk of certain crimes such as drunk driving, spousal abuse and fighting. Around 60% of an individual’s alcohol use is attributable to genetic influences, however little is known regarding the specific genes that are involved in increasing the risk of risky alcohol use. Recent theories posit that some genes are so called susceptibility genes, meaning that carriers of certain genes or alleles are more susceptible both to positive and negative environments. The aim of the present study was to examine main and interaction effects of a possible susceptibility gene (
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Books on the topic "Usp gene"

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Dr, Cooper David N., and Lemoine Nicholas R, eds. Gene therapy. Bios Scientific Publishers, 1996.

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Arslanoglu, Alper. Studies of the adenovirus 5 L1 gene aimed at developing L1 gene deficiencies for use in gene therapy vectors. typescript, 1999.

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Miller, Davin Philip. Characterisation of the entomopoxvirus spheroidin gene promoter and its use in heterologous gene expression. Oxford Brookes University, 1995.

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1959-, Rolland Alain, and Sullivan Sean M, eds. Pharmaceutical gene delivery systems. M. Decker, 2003.

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A, Lidbury Brett, and Mahalingam Suresh, eds. Gene profiling in drug design. Taylor & Francis, 2008.

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I, Mahato Ram, and Kim Sung Wan, eds. Pharmaceutical perspectives of nucleic acid-based therapeutics. Taylor & Francis, 2002.

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Great Britain. Gene Therapy Advisory Committee. Report on the potential use of gene therapy in utero. Department of Health, 1998.

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Rho, Jong M. Epilepsy: Mechanisms, models, and translational perspectives. CRC Press, 2010.

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R, Bertolotti, and Ozawa Keiya 1953-, eds. Autologous and cancer stem cell gene therapy. World Scientific, 2008.

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J, Ferretti Joseph, American Society for Microbiology, International Association of Biological Standardization., and International ASM Conference on Streptococcal Genetics (4th : 1994 : Santa Fe, N.M.), eds. Genetics of streptococci, enterococci, and lactococci: Santa Fee [i.e. Fe], NM, USA, May 15-18, 1994. Karger, 1995.

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Book chapters on the topic "Usp gene"

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Ray, Sujay, and Arundhati Banerjee. "Exploring the Human USP Gene Family and Its Association with Cancer: An In Silico Study." In Advances in Data Science and Computing Technologies. Springer Nature Singapore, 2023. http://dx.doi.org/10.1007/978-981-99-3656-4_70.

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Akpolat, Veysi. "Gene Therapy Techniques;Physical and Chemical Methods." In Gene Therapy. Nobel Tip Kitabevleri, 2024. http://dx.doi.org/10.69860/nobel.9786053358824.2.

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Gene therapy is a technique that changes a person’s genes to treat or cure disease. To insert new genes directly into cells, scientists use a tool called a “vector.” Vectors are genetically engineered to deliver the genes needed to treat the disease. Various approaches can be used to deliver DNA into the cell with different gene transfer techniques. It is divided into two categories: 1. Biological vectors 2. Physical and Chemical methods. With gene transfers, the chance of treatment for diseases caused by defective genes increases.
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Gondro, Cedric. "Gene Expression Analysis." In Use R! Springer International Publishing, 2015. http://dx.doi.org/10.1007/978-3-319-14475-7_5.

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Running, Katherine L. D., and Justin D. Faris. "Rapid Cloning of Disease Resistance Genes in Wheat." In Compendium of Plant Genomes. Springer International Publishing, 2023. http://dx.doi.org/10.1007/978-3-031-38294-9_10.

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AbstractWheat is challenged by rapidly evolving pathogen populations, resulting in yield losses. Plants use innate immune systems involving the recognition of pathogen effectors and subsequent activation of defense responses to respond to pathogen infections. Understanding the genes, genetic networks, and mechanisms governing plant-pathogen interactions is key to the development of varieties with robust resistance whether through conventional breeding techniques coupled with marker selection, gene editing, or other novel strategies. With regards to plant-pathogen interactions, the most useful
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Smulders, Marinus J. M., Luud J. W. J. Gilissen, Martina Juranić, Jan G. Schaart, and Clemens C. M. van de Wiel. "Gene Editing of Wheat to Reduce Coeliac Disease Epitopes in Gluten." In A Roadmap for Plant Genome Editing. Springer Nature Switzerland, 2023. http://dx.doi.org/10.1007/978-3-031-46150-7_13.

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AbstractBy using gene editing technologies such as CRISPR/Cas, precise modifications can be made in the genome. CRISPR/Cas is especially valuable for targeted mutagenesis in polyploids, as it can induce mutations of multiple alleles simultaneously, to obtain regenerants that are homozygous for the desired mutation. A range of gene-edited traits have been developed in hexaploid bread wheat, including various nutrition and health-related traits, plant architecture, pest and disease resistance, tolerance to abiotic stress, and traits that enable more efficient breeding. Wheat is also known as a c
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Housman, David E., and David L. Nelson. "Use of Metaphase-Chromosome Transfer for Mammalian Gene Mapping." In Gene Transfer. Springer US, 1986. http://dx.doi.org/10.1007/978-1-4684-5167-2_4.

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Danos, Olivier. "Adeno-Associated Viral Vectors: Principles and in vivo Use." In Gene Therapy. Springer Berlin Heidelberg, 1998. http://dx.doi.org/10.1007/978-3-642-72160-1_11.

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Ledley, Fred D. "Pharmacokinetic Considerations in the Use of Genes as Pharmaceuticals." In Gene Therapeutics. Birkhäuser Boston, 1994. http://dx.doi.org/10.1007/978-1-4684-6822-9_13.

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Kokošar, Jaka, Martin Špendl, and Blaž Zupan. "Gene Interactions in Survival Data Analysis: A Data-Driven Approach Using Restricted Mean Survival Time and Literature Mining." In Discovery Science. Springer Nature Switzerland, 2023. http://dx.doi.org/10.1007/978-3-031-45275-8_20.

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AbstractUnveiling gene interactions is crucial for comprehending biological processes, particularly their combined impact on phenotypes. Computational methodologies for gene interaction discovery have been extensively studied, but their application to censored data has yet to be thoroughly explored. Our work introduces a data-driven approach to identifying gene interactions that profoundly influence survival rates through the use of survival analysis. Our approach calculates the restricted mean survival time (RMST) for gene pairs and compares it against their individual expressions. If the int
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Silan, Coskun, and Buket GüNgor. "Licensing Processes for Gene Therapy Products: Approved and Pending Clinical Trials in the World and Turkey." In Gene Therapy. Nobel Tip Kitabevleri, 2024. http://dx.doi.org/10.69860/nobel.9786053358824.10.

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Gene therapy is a medical technology that aims to treat diseases by alteration, insertion or correction of genes. Gene therapy offers potential in treating many genetic diseases such as cystic fibrosis, blood cancers and neurological disorders. Preclinical processes include laboratory research, vector selection, gene editing studies and animal experiments. Clinical research phases consist of four phases: Phase I, safety and tolerability; Phase II, clinical efficacy and best dosages; Phase III, efficacy, safety and availability; Phase IV evaluates long-term effects and rare side effects. Gene t
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Conference papers on the topic "Usp gene"

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Biktasheva, Liliya, Nikita Ezhkin, Zlata Osmorskaya, Alexander Gordeev, and Polina Kuryntseva. "APPLICATION OF CULTURE SUPERNATANTS CONTAINING SURFACTANTS TO ENHANCE OIL RECOVERY." In 24th SGEM International Multidisciplinary Scientific GeoConference 24. STEF92 Technology, 2024. https://doi.org/10.5593/sgem2024/1.1/s06.67.

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Currently, a big problem is increasing the production of residual oil, for which so-called tertiary recovery methods are used. One common tertiary recovery method is the use of surfactants. The use of biological surfactants instead of synthetic ones can solve the problem of increasing oil production and reducing the toxic impact on the environment. To obtain biosurfactants, two strains of Bacillus mojavensis and Bacillus amyloliquefaciens were used. These strains are characterized by the ability to produce biosurfactants. Biosurfactants produced by these strains belonged to the group of lipope
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Hubel, Allison, and Jeffrey McCullough. "Cryopreservation of Cultured Blood Cells for Use in Gene Therapy and Immunotherapy." In ASME 1997 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 1997. http://dx.doi.org/10.1115/imece1997-1318.

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Abstract The freezing characteristics of genetically manipulated lymphocytes and hematopoietic stem cells were studied. The water transport characteristics of stem cells which had been cultured and transduced with a therapeutic gene were different from that of a freshly isolated cells. Changes in the freezing characteristics for genetically transformed lymphocytes were also observed. Specifically, cryopreservation protocols developed for the fresh peripheral blood lymphocytes do not produce comparable survival rates for lymphocytes which have been cultured and transduced. These results indicat
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Temple, Mark D. "DNA Sonification Using 8-Channel Audio for Data Analyses and Music Composition." In ICAD 2024: The 29th International Conference on Auditory Display. International Community for Auditory Display, 2024. http://dx.doi.org/10.21785/icad2024.016.

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DNA sequences contain vast amounts of biological data and computer algorithms play an important role in processing these data for human inspection. Here we describe an updated computer-generated auditory display tool to be used as stand-alone audio or as a complement to a visual display for DNA sequence inspection. The auditory display uses musical notes to represent the data in relation to the process of gene expression or DNA replication. Given the use of musical notes in the auditory display raises the possibility these may be considered algorithmic music. To pursue this notion further the
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Le Borgne, S., J. M. Romero, H. A. Videla, J. M. Gonzalez, and C. Saiz-Jiménez. "Practical Cases of the Use of Molecular Techniques to Characterize Microbial Deterioration of Metallic Structures in Industry." In CORROSION 2007. NACE International, 2007. https://doi.org/10.5006/c2007-07523.

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Abstract Two specific cases of applying molecular techniques for deciphering the role of microorganisms in industrial processes are presented: an offshore seawater injection system and a wastewater treatment plant. In the first case, deoxyribonucleic acid (DNA) was extracted from a water sample taken from an offshore seawater injection system and from enrichment cultures from the same sample. The V3 hypervariable region of the 16S rDNA gene was amplified by the polymerase chain reaction (PCR) and bacterial diversity was studied using denaturing gel gradient electrophoresis (DGGE). DGGE monitor
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Lopes, Camila Galvão, Gabriele Campos, Jaqueline Wang, Ana Cristina Girardi, and Maria Rita Passos Bueno. "Characterization of de novo variants in exomes of individuals with autism spectrum disorder." In XIV Congresso Paulista de Neurologia. Zeppelini Editorial e Comunicação, 2023. http://dx.doi.org/10.5327/1516-3180.141s1.564.

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The main objective of this essay was to contribute to the characterization of the genetic architecture of autism spectrum disorder (ASD) based on an analysis of a Brazilian series, which is still little studied. To achieve this goal, we verified the proportion of cases of ASD caused by de novo variants in neurodevelopment genes (genes from the SFARI bank and those associated with neurodevelopment described in the DECIPHER bank). Sixty-three trios were evaluated, composed of parents and probands diagnosed with ASD treated at the Human Genome and Stem Cell Studies Center (CEGH-CEL, USP). Genealo
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Assato, MA, DM Lopes, MFD Silva, et al. "ANÁLISE COMPARATIVA ENTRE TRÊS DIFERENTES PROTOCOLOS: CDC DA CHINA, NIHS DO JAPÃO E CDC DOS EUA, POR QRTPCR (PADRÃO-OURO) NO DIAGNÓSTICO DE COVID-19, EM DUAS PLATAFORMAS DISTINTAS, BD MAX E QIAQUANT 5 PLEX, ENTRE OS PERÍODOS DE 2020 A 2023." In Resumos do 55º Congresso Brasileiro de Patologia Clínica/Medicina Laboratorial. Zeppelini Editorial e Comunicação, 2023. http://dx.doi.org/10.5327/1516-3180.141s2.7762.

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Objetivo: O padrão-ouro de diagnóstico para Covid-19 é a metodologia de amplificação de ácidos nucleicos do vírus Sars-CoV-2, conhecida como qRT-PCR. O objetivo deste trabalho foi comparar duas plataformas de qRT-PCR, utilizando três protocolos distintos, recomendados pela Organização Mundial da Saúde (OMS) para o diagnóstico da doença, o CDC da China e dos EUA e o NIHS do Japão. Método: Neste trabalho, os protocolos foram padronizados, avaliados e analisados em uma plataforma automatizada de extração, amplificação e detecção de ácidos nucleicos, o BD Max. O melhor resultado foi submetido à pl
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Rivera, Grecia C. G., Juan G. Colonna, and Marcelo Ruiz. "Discovery of Conditionally Independent Networks Among Gene Expressions in Breast Cancer Using Fast Step Graph." In Simpósio Brasileiro de Computação Aplicada à Saúde. Sociedade Brasileira de Computação - SBC, 2025. https://doi.org/10.5753/sbcas.2025.7499.

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The heterogeneity of the causes of breast cancer and these complex gene interactions that characterize this neoplasm present significant challenges to understanding and treating the disease. This study is motivated by the need to identify interconnected networks of breast cancer genes, specifically those that represent conditional independence relationships. To construct these networks, we propose the use of the Fast Step Graph algorithm, which belongs to the family of sparse, high-dimensional Gaussian Graphical Models, applied to the PAM50 gene expression dataset. This dataset was stratified
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Surjikova, E,, D. Evlagyna та O. Onyshenko. "Полиморфизм генов GHR и IGF-1 у ремонтного молодняка казахской белоголовой породы". У Scientific and practical conference with international participation: "Management of the genetic fund of animals – problems, solutions, outlooks". Scientific Practical Institute of Biotechnologies in Animal Husbandry and Veterinary Medicine, 2023. http://dx.doi.org/10.61562/mgfa2023.47.

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It is known that meat productivity and meat quality in cattle are determined not only by paratypic, but also by genetic factors, therefore, the use of genetic markers is relevant and promising. The aim of the study was to determine gene polymorphism of growth hormone receptor (GHR) and insulin-like growth factor (IGF-1) and to carry out genetic and statistical analysis in a sample of young cattle (steers) of beef cattle of Kazakh white-headed breed. The allelic spectrum of the genes under study was determined by PCR-PDRF method. Research showed that in this sample of animals, the following gen
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Prates, Pedro Emílio Gomes. "AVALIAÇÃO DA TERAPIA GÊNICA DO SUICÍDIO COM USO DE GENES SUICIDAS PARA O COMBATE AO CÂNCER: REVISÃO INTEGRATIVA." In II Congresso Brasileiro de Biologia Molecular On-line. Revista Multidisciplinar em Saúde, 2021. http://dx.doi.org/10.51161/rems/2334.

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Introdução: Durante décadas os tratamentos quimioterápicos convencionais foram empregados no câncer devido à eficácia em matar as células tumorais. Diante disso, a terapia gênica com uso de genes suicidas surge como uma das abordagens mais inovadoras para o desenvolvimento de agentes antineoplásicos com maior seletividade tumoral. Assim, entre os genes suicidas disponíveis, a timidina quinase é investigada em vários modelos de tumor. Contudo, o uso dessa enzima apresenta limitações devido ao sistema de imunogenicidade. Recentemente, pesquisadores passaram a utilizar o sistema de gene suicida d
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Stacey, Minviluz. "Utility of CRISPR/Cas in accelerating gene discovery in soybean." In 2022 AOCS Annual Meeting & Expo. American Oil Chemists' Society (AOCS), 2022. http://dx.doi.org/10.21748/rzne1660.

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The use of CRISPR/Cas9 has been successfully applied in various plant species to induce targeted genome editing, including soybean. Soybean is recalcitrant to transformation and thus, plants with stable CRISPR gene edits are costly and take a long time to produce. Moreover, soybean is allotetraploid and editing paralogous genes are often necessary to obtain observable phenotype(s). For each gene target, we designed two gRNAs to increase editing efficiency and allow rapid genotyping by PCR. We also tested the CRISPR reagents in transient hairy root transformation to determine if the Cas9 and gR
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Reports on the topic "Usp gene"

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Reisch, Bruce, Avichai Perl, Julie Kikkert, Ruth Ben-Arie, and Rachel Gollop. Use of Anti-Fungal Gene Synergisms for Improved Foliar and Fruit Disease Tolerance in Transgenic Grapes. United States Department of Agriculture, 2002. http://dx.doi.org/10.32747/2002.7575292.bard.

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Original objectives . 1. Test anti-fungal gene products for activity against Uncinula necator, Aspergillus niger, Rhizopus stolonifer and Botrytis cinerea. 2. For Agrobacterium transformation, design appropriate vectors with gene combinations. 3. Use biolistic bombardment and Agrobacterium for transformation of important cultivars. 4. Characterize gene expression in transformants, as well as level of powdery mildew and Botrytis resistance in foliage of transformed plants. Background The production of new grape cultivars by conventional breeding is a complex and time-consuming process. Transfer
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Meir, Shimon, Michael Reid, Cai-Zhong Jiang, Amnon Lers, and Sonia Philosoph-Hadas. Molecular Studies of Postharvest Leaf and Flower Abscission. United States Department of Agriculture, 2005. http://dx.doi.org/10.32747/2005.7696523.bard.

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Original objectives: Understanding the regulation of abscission competence by exploring the nature and function of auxin-related gene expression changes in the leaf and pedicelAZs of tomato (as a model system), was the main goal of the previously submitted proposal. We proposed to achieve this goal by using microarray GeneChip analysis, to identify potential target genes for functional analysis by virus-induced gene silencing (VIGS). To increase the potential of accomplishing the objectives of the previously submitted proposal, we were asked by BARD to show feasibility for the use of these two
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Harman, Gary E., and Ilan Chet. Discovery and Use of Genes and Gene Combinations Coding for Proteins Useful in Biological Control. United States Department of Agriculture, 1994. http://dx.doi.org/10.32747/1994.7568787.bard.

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The objectives of the research in this proposal were to (A) identify synergy among proteins that provide enhanced activity over single proteins for control of plant pathogenic fungi, (B) clone and characterize genetic sequences coding for proteins with ability to control pathogenic fungi, (C) produce transgenic organisms with enhanced biocontrol ability using genes and gene combinations and determine their efficiency in protecting plants against plant pathogenic fungi. A related objective was to produce disease-resistant plants. Fungal cell wall degrading enzymes from any source are strongly s
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Sengupta-Gopalan, Champa, Shmuel Galili, and Rachel Amir. Improving Methionine Content in Transgenic Forage Legumes. United States Department of Agriculture, 2001. http://dx.doi.org/10.32747/2001.7580671.bard.

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Leguminous forage crops are high in proteins but deficient in S- amino acids. It has been shown that both wool quality and milk production can be limited by the post-ruminal supply of sulfur-containing amino acids. Efforts to use conventional plant breeding and cell selection techniques to increase the S-amino acid content of alfalfa have met with little success. With the objective to increase the S-amino acid content of forage legumes, the goal of this project was to co- express the methionine rich zein genes from corn along with a gene for a key enzyme in methionine biosynthesis, aspartate k
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Fromm, A., Avihai Danon, and Jian-Kang Zhu. Genes Controlling Calcium-Enhanced Tolerance to Salinity in Plants. United States Department of Agriculture, 2003. http://dx.doi.org/10.32747/2003.7585201.bard.

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The specific objectives of the proposed research were to identify, clone and characterize downstream cellular target(s) of SOS3 in Arabidopsis thaliana, to analyze the Ca2+-binding characteristics of SOS3 and the sos3-1 mutant and their interactions with SOS3 cellular targets to analyze the SOS3 cell-specific expression patterns, and its subcellular localization, and to assess the in vivo role of SOS3 target protein(s) in plant tolerance to salinity stress. In the course of the study, in view of recent opportunities in identifying Ca2+ - responsive genes using microarrays, the group at Weizman
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Eshed, Y., and Z. B. Lippman. Fine tuning the shoot and inflorescence architectures for improved tomato yield. United States-Israel Binational Agricultural Research and Development Fund, 2022. http://dx.doi.org/10.32747/2022.8134148.bard.

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In this project, we are determining the contribution of different types of variations, in gene function and in gene regulation, to altered shoot architecture first, and to field performance in the next stage. We are using tomato as a target, but also as a model for many other crops. Our focus is on two different components of yield associated traits - shoot architecture and organization of the inflorescence. Our focus was on two types of regulators; 1) genes involved in florigen - antiflogen balance and the way they impact the shoot, and 2) genes involved in inflorescence branching and it this
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Dubcovsky, Jorge, Tzion Fahima, Ann Blechl, and Phillip San Miguel. Validation of a candidate gene for increased grain protein content in wheat. United States Department of Agriculture, 2007. http://dx.doi.org/10.32747/2007.7695857.bard.

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High Grain Protein Content (GPC) of wheat is important for improved nutritional value and industrial quality. However, selection for this trait is limited by our poor understanding of the genes involved in the accumulation of protein in the grain. A gene with a large effect on GPC was detected on the short arm of chromosome 6B in a Triticum turgidum ssp. dicoccoides accession from Israel (DIC, hereafter). During the previous BARD project we constructed a half-million clones Bacterial Artificial Chromosome (BAC) library of tetraploid wheat including the high GPC allele from DIC and mapped the G
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Levin, Ilan, Avtar K. Handa, Avraham Lalazar, and Autar K. Mattoo. Modulating phytonutrient content in tomatoes combining engineered polyamine metabolism with photomorphogenic mutants. United States Department of Agriculture, 2006. http://dx.doi.org/10.32747/2006.7587724.bard.

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Fruit constitutes a major component of our diet, providing fiber, vitamins, minerals, and many other phytonutrients that promote good health. Fleshy fruits, such as tomatoes, already contain high levels of several of these ingredients. Nevertheless, efforts have been invested in increasing and diversifying the content of phytonutrients, such as carotenoids and flavonoids, in tomato fruits. Increasing levels of phytonutrients, such as lycopene, is highly justified from the perspective of the lycopene extraction industry due to cost effectiveness reasons. Diversifying phytonutrients, in particul
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Palukaitis, Peter, Amit Gal-On, Milton Zaitlin, and Victor Gaba. Virus Synergy in Transgenic Plants. United States Department of Agriculture, 2000. http://dx.doi.org/10.32747/2000.7573074.bard.

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Transgenic plants expressing viral genes offer novel means of engendering resistance to those viruses. However, some viruses interact synergistically with other viruses and it is now known that transgenic plants expressing particular genes of one virus may also mediate synergy with a second virus. Thus, our specific objectives were to (1) determine if transgenic plants resistant to one virus showed synergy with another virus; (2) determine what viral sequences were essential for synergy; and (3) determine whether one of more mechanisms were involved i synergy. This project would also enable an
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Paran, Ilan, and Molly Jahn. Analysis of Quantitative Traits in Pepper Using Molecular Markers. United States Department of Agriculture, 2000. http://dx.doi.org/10.32747/2000.7570562.bard.

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Original objectives: The overall goal of the proposal was to determine the genetic and molecular control of pathways leading to the production of secondary metabolites determining major fruit quality traits in pepper. The specific objectives were to: (1) Generate a molecular map of pepper based on simple sequence repeat (SSR) markers. (2) Map QTL for capsaicinoids content (3) Determine possible association between capsaicinoids and carotenoid content and structural genes for capsaicinoid and carotenoid biosynthesis. (4) Map QTL for quantitative traits controlling additional fruit traits. (5) M
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